BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3504.p (589 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312632|ref|YP_003776724.1| dihydroxyacid dehydratase/phosp... 514 e-147 gi|300310373|ref|YP_003774465.1| dihydroxyacid dehydratase/phosp... 485 e-138 gi|300313780|ref|YP_003777872.1| dihydroxyacid dehydratase/phosp... 325 7e-90 gi|300309867|ref|YP_003773959.1| dihydroxyacid dehydratase/phosp... 206 5e-54 gi|300310433|ref|YP_003774525.1| 6-phosphogluconate dehydratase ... 151 1e-37 >gi|300312632|ref|YP_003776724.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 578 Score = 514 bits (1324), Expect = e-147, Method: Compositional matrix adjust. Identities = 264/575 (45%), Positives = 376/575 (65%), Gaps = 3/575 (0%) Query: 7 SEEKQKPLLRSARWFRKNDLPGFIHRSTLAADGWTREDLMEKPIVGILNTWSDINPCNMN 66 +++ +K RS WF + D GFI+RS + G + +P++GI NT+S++ PCN + Sbjct: 2 TDDTKKSTRRSQAWFGRQDRDGFIYRSWVKNRGIPHDQFDGRPVIGICNTYSELTPCNSH 61 Query: 67 LRMLAQKVREGILEAGGIPFEIPLMSISENLIKPSSFPFRNMLAMDTEECIRGYPLDAVV 126 R LA++V+ GI EAGG P E P+MS+ E L++P++ +RN+ +MD EE IR PLD VV Sbjct: 62 FRALAEQVKIGIWEAGGFPLEFPVMSLGETLLRPTAMLYRNLASMDVEESIRANPLDGVV 121 Query: 127 LMGGCDKTQPGLLMGAASANVPAIALNSGPGTPRSAIGGPLATANGVWRFVDQLRAGCLS 186 L+ GCDKT P LLMGAAS +VP I ++ GP G L + GVW+ + +RAG ++ Sbjct: 122 LLMGCDKTTPALLMGAASVDVPTIGVSGGPMLSGKYRGRELGSGTGVWQMSEDVRAGKMT 181 Query: 187 TESFENFERQTMGSPGHCSEMGTASTMSSISEALGLALPGSSLIPSSDGRRSQIAKAVGR 246 E F E S GHC MGTASTM+S+ EALG+ LP ++ IP+ D RR+ +A+ GR Sbjct: 182 QEEFFEAESCMHRSHGHCMTMGTASTMASMVEALGVGLPHNAAIPAVDARRNVLARNAGR 241 Query: 247 CSVALALSGDLAPANILDARAFDNAITLLCAVGGSTNAIIHLLALAGRVGVPLTLDRFDE 306 V + + DL + IL +AF+NAI + A+GGSTNA+IHLLA+AGR+G+ L L +D+ Sbjct: 242 RIVQM-VEEDLVLSKILTRQAFENAIRVNAAIGGSTNAVIHLLAIAGRIGIKLDLKDWDD 300 Query: 307 IARRVPLISNVQPAGEHLTERLMNAGGVPALLHVLRPLLHLDARTVDGRTIGEIADQAEV 366 I +++P + ++QP+G+ L E AGG+PA++ L ++ A T +G+T+ E A Sbjct: 301 IGQQLPCLLDLQPSGKFLMEDFYYAGGLPAVIRQLESVIDKTALTANGKTLWENCQDAPN 360 Query: 367 SDTDVIRQLDTPVKPGPSLVVVKGNLAPHGAVIKSCAASPALFKHRGAAVVFEDVQTLAD 426 + +VIR D P K + ++KGNLAP GAVIK AA+PAL KHRG AVVFE+ L Sbjct: 361 WNEEVIRSFDKPFKEAAGIAILKGNLAPDGAVIKPSAATPALLKHRGRAVVFENSDDLHK 420 Query: 427 TIDDPTMNIDANSVLILRNVGPVGA-AMPEWGMLPLPRRLMEHGVKDMLRISDARMSGTA 485 IDD +++D VL+L+N GP G M E G +PLP +++ G+ DM+R+SDARMSGTA Sbjct: 421 RIDDENLDVDETCVLVLKNCGPKGYPGMAEAGNMPLPPKILRKGITDMVRVSDARMSGTA 480 Query: 486 YGTSILHVSPEAAVGGPLSLVRTGDIVELDVSERRLELIVSEEILAQRRKETPQHAPSPH 545 YGT +LHVSPEAA GGPL++V+ GD +ELDV R+L L VS+E LA+RR + + P Sbjct: 481 YGTVVLHVSPEAAAGGPLAVVQNGDFIELDVEARKLHLDVSDEELARRRAQWQKPELPPQ 540 Query: 546 V-RGYRQLYGREVEQAHLGCDFKFLRGSNANVLPE 579 + RG+ +LY V+QA+ G D FL G + +P+ Sbjct: 541 MQRGWVKLYVDHVQQANQGADLDFLVGKSGPYVPK 575 >gi|300310373|ref|YP_003774465.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 585 Score = 485 bits (1249), Expect = e-138, Method: Compositional matrix adjust. Identities = 263/581 (45%), Positives = 366/581 (62%), Gaps = 9/581 (1%) Query: 7 SEEKQKP-LLRSARWFRKNDLPGFIHRSTLAADGWTREDLMEKPIVGILNTWSDINPCNM 65 S++K K LRSA WF D GF++RS + G + KP++GI NTWS++ PCN Sbjct: 3 SDKKDKSRTLRSAGWFGTADKNGFMYRSWMKNQGIPDHEFQGKPVIGICNTWSELTPCNA 62 Query: 66 NLRMLAQKVREGILEAGGIPFEIPLMSISENLIKPSSFPFRNMLAMDTEECIRGYPLDAV 125 + R +A+ VR GI+EAGG P E P+ S E+ ++P++ RN+ +MD EE IRG P+DAV Sbjct: 63 HFRKIAEHVRRGIIEAGGFPVEFPVFSNGESNLRPTAMLTRNLASMDVEESIRGNPIDAV 122 Query: 126 VLMGGCDKTQPGLLMGAASANVPAIALNSGPGTPRSAIGGPLATANGVWRFVDQLRAGCL 185 VL+ GCDKT P LLMGAAS +VPAI + GP G + + VW+ +Q++AG + Sbjct: 123 VLLTGCDKTTPALLMGAASCDVPAIVVTGGPMLNGKHQGRDIGSGTVVWQLSEQVKAGEI 182 Query: 186 STESFENFERQTMGSPGHCSEMGTASTMSSISEALGLALPGSSLIPSSDGRRSQIAKAVG 245 + F E S G C+ MGTASTM+ ++E+LG++LP ++ IP+ D RR +A G Sbjct: 183 TIHDFMAAEAGMSRSAGTCNTMGTASTMACMAESLGVSLPHNAAIPAVDARRYVLAHLSG 242 Query: 246 RCSVALALSGDLAPANILDARAFDNAITLLCAVGGSTNAIIHLLALAGRVGVPLTLDRFD 305 V + G L + IL +AF+NAI A+GGSTNA+IHL A+AGR+GV L L+ + Sbjct: 243 MRIVDMVWEG-LTLSKILTRKAFENAIRTNAAIGGSTNAVIHLKAIAGRIGVDLELEDWT 301 Query: 306 EIARRVPLISNVQPAGEHLTERLMNAGGVPALLHVLRP---LLHLDARTVDGRTIGEIAD 362 I R P I ++QP+G +L E AGG+PA+L L L H DA TV+G+T+ + Sbjct: 302 RIGRGTPTIVDLQPSGRYLMEEFYYAGGLPAVLRRLGEADLLPHKDALTVNGQTMWDNVK 361 Query: 363 QAEVSDTDVIRQLDTPVKPGPSLVVVKGNLAPHGAVIKSCAASPALFKHRGAAVVFEDVQ 422 A + + +V+R L P+ + +++GNLAP GAV+K AA+P L KHRG AVVFED Sbjct: 362 DAPIYNDEVVRPLAKPLIEDGGICILRGNLAPRGAVLKPSAATPELMKHRGRAVVFEDFN 421 Query: 423 TLADTIDDPTMNIDANSVLILRNVGPVGA-AMPEWGMLPLPRRLMEHGVKDMLRISDARM 481 + I+DP +++DA+ VL+++NVGP G M E G + LP +++ GVKDM+RISDARM Sbjct: 422 HYKERINDPDLDVDASCVLVMKNVGPKGYPGMAEVGNMGLPPKVLATGVKDMVRISDARM 481 Query: 482 SGTAYGTSILHVSPEAAVGGPLSLVRTGDIVELDVSERRLELIVSEEILAQR---RKETP 538 SGTAYGT ILHV+PEAA GGPL +V+ GD +ELD +L+L +SEE + +R R + Sbjct: 482 SGTAYGTVILHVAPEAAAGGPLGIVQDGDFIELDAYAGKLQLDISEEEMKRRLEARAKVL 541 Query: 539 QHAPSPHVRGYRQLYGREVEQAHLGCDFKFLRGSNANVLPE 579 V GY+ LY V QA GCDF FL G +P+ Sbjct: 542 AERKPEMVGGYQSLYVDRVLQADEGCDFDFLVGCRGAAVPK 582 >gi|300313780|ref|YP_003777872.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 594 Score = 325 bits (833), Expect = 7e-90, Method: Compositional matrix adjust. Identities = 216/573 (37%), Positives = 314/573 (54%), Gaps = 32/573 (5%) Query: 15 LRSARWFRKNDLPGFIHRSTLAAD-----GWTREDLME-KPIVGILNTWSDINPCNMNLR 68 RS WF D P I + L + G T E+L +PI+GI + SDI+PCN Sbjct: 11 FRSQDWF---DNPDHIDMTALYLERFMNYGITAEELRSGRPIIGIAQSGSDISPCNRIHL 67 Query: 69 MLAQKVREGILEAGGIPFEIPLMSISENLIKPSSFPFRNMLAMDTEECIRGYPLDAVVLM 128 LA++VR+GI +AGGIP E PL I EN +P++ RN+ + E + GYP+DAVVL Sbjct: 68 ELAKRVRDGIRDAGGIPMEFPLHPIFENCRRPTAAIDRNLAYLGLVEILHGYPIDAVVLT 127 Query: 129 GGCDKTQPGLLMGAASANVPAIALNSGPGTPRSAIGGPLATANGVWRFVDQLRAGCLSTE 188 GCDKT P +M AA+ ++PAI L+ GP G + + + +W+ L AG + E Sbjct: 128 TGCDKTTPSQIMAAATVDIPAIVLSGGPMLDGWMDGELVGSGSAIWKGRKLLSAGSIDNE 187 Query: 189 SFENFERQTMGSPGHCSEMGTASTMSSISEALGLALPGSSLIPSSDGRRSQIAKAVGRCS 248 F + S GHC+ MGTASTM++++EALG++L G S IP+ R Q+A GR Sbjct: 188 KFLEIAAASAPSSGHCNTMGTASTMNAMAEALGMSLTGCSAIPAPYRERGQMAYETGRRI 247 Query: 249 VALALSGDLAPANILDARAFDNAITLLCAVGGSTNAIIHLLALAGRVGVPLTLDRFDEIA 308 V +A DL P+ IL AF +AI + A+GGSTNA H++A+A GV L + + + Sbjct: 248 VGMAYE-DLRPSAILTRDAFLDAIVVNAAIGGSTNAQPHIMAMARHAGVELQSEDWMKYG 306 Query: 309 RRVPLISNVQPAGEHLTERLMNAGGVPALLHVLRPLLHLDAR--TVDGRTIGEIADQAEV 366 VPL+ N+QPAG++L ER AGGVPA++ L+ L A T G+T+ E Sbjct: 307 YDVPLLLNMQPAGKYLGERFHRAGGVPAIMWELQQAGKLRAERITATGKTMAENLQGRAS 366 Query: 367 SDTDVIRQLDTPVKPGPSLVVVKGNLAPHGAVIKSCAASPA-------------LFKHRG 413 +D ++I P++ +V+KGNL A++K+ S +F+ R Sbjct: 367 NDREMIYPFAAPLRERAGFLVLKGNLFDF-AIMKTSVISETFRERYLSTPGQENIFECR- 424 Query: 414 AAVVFEDVQTLADTIDDPTMNIDANSVLILRNVGPVG--AAMPEWGMLPLPRRLMEHGVK 471 AVVF+ I+DP + ID N++L +R GPVG + M P P L++ GV Sbjct: 425 -AVVFDGSDDYHARINDPALKIDENTLLAIRGAGPVGWPGSAEVVNMQP-PDALIKRGVS 482 Query: 472 DMLRISDARMSGTAYGTSILHVSPEAAVGGPLSLVRTGDIVELDVSERRLELIVSEEILA 531 + + D R SGT+ SIL+ SPE+AVGG L+ +R GD V +D++ ++VSEE LA Sbjct: 483 TLPTLGDGRQSGTSDSPSILNASPESAVGGGLAYLRDGDRVRIDLNTGECNMLVSEEELA 542 Query: 532 QRRKETPQHAPSPHVRGYRQLYGREVEQAHLGC 564 +R+ E P P ++++Y V Q G Sbjct: 543 RRKSEGIPPVP-PSQTPWQEIYRSTVGQLETGA 574 >gi|300309867|ref|YP_003773959.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 620 Score = 206 bits (524), Expect = 5e-54, Method: Compositional matrix adjust. Identities = 174/610 (28%), Positives = 275/610 (45%), Gaps = 72/610 (11%) Query: 13 PLLRSARWFRKNDLPGFIHRSTLAADGWTREDLMEKPIVGILNTWSDINPCNMNLRMLAQ 72 P RS + ++ G R+ A G D KPI+ ++N+++ P +++L+ L Q Sbjct: 2 PTYRSYTTTQGRNMAG--ARALWRATGMKDGDFT-KPIIAVVNSFTQFVPGHVHLKDLGQ 58 Query: 73 KVREGILEAGGIPFEIPLMSISENLIKPS-----SFPFRNMLAMDTEECIRGYPLDAVVL 127 V I AGG+ E +++ ++ S P R+++A E + + DA+V Sbjct: 59 LVAREIEAAGGVAKEFNTIAVDDSGTHGDARMLYSLPSRDLIADSVEYMVNAHCADAMVC 118 Query: 128 MGGCDKTQPGLLMGAASANVPAIALNSGPGTPRSAIGGPLATANGVWRFVD------QLR 181 + CDK PG+LM A N+P + ++ GP G + T N + + ++ Sbjct: 119 ISNCDKITPGMLMAAMRLNIPVVFVSGGP----MEAGKVIKTVNTDKKVIKLDLVDAMIQ 174 Query: 182 AG--CLSTESFENFERQTMGSPGHCSEMGTASTMSSISEALGLALPGSSLIPSSDGRRSQ 239 AG +S ER + G CS M TA++M+ ++E LGL+LPG+ I ++ R + Sbjct: 175 AGDKNISDADVAEVERSACPTCGSCSGMFTANSMNCLTEVLGLSLPGNGTIVATHADRKE 234 Query: 240 IAKAVGRCSVALAL------SGDLAPANILDARAFDNAITLLCAVGGSTNAIIHLLALAG 293 + GR V LA + P NI ++NA+TL ++GGSTN ++HLLA A Sbjct: 235 LFLRAGRLIVELAKRHYEQDDYSILPRNICTKETWENAMTLDVSMGGSTNTVLHLLAAAH 294 Query: 294 RVGVPLTLDRFDEIARRVPLISNVQP-AGEHLTERLMNAGGVPALLHVLRPLLHLDAR-- 350 V T+ D I+RRVP + V P ++ E + AGG+ ++L L LD Sbjct: 295 EAKVEFTMADIDRISRRVPCLCKVAPMTNKYHIEDVHRAGGIISILGELARAGLLDTSRP 354 Query: 351 TVDGRTIGEIADQ---------------------------------AEVSDTD----VIR 373 TV +T+GE ++ E +D D IR Sbjct: 355 TVHSKTLGEAIEKFDIRVSSDPAVHKLFRAAPGGVPTQVAFSQEERFEANDLDRANGCIR 414 Query: 374 QLDTPVKPGPSLVVVKGNLAPHGAVIKSCAASPALFKHRGAAVVFEDVQTLADTIDDPTM 433 + L V+ GN+A G ++K+ ++ K G A VFE + I Sbjct: 415 DREHAYSQDGGLAVLYGNIAEKGCIVKTAGVDESILKFSGTARVFESQDAAVEGILGD-- 472 Query: 434 NIDANSVLILRNVGPVGA-AMPEWGMLPLPRRLMEHGV-KDMLRISDARMSGTAYGTSIL 491 + A V+I+R GP G M E ML + G+ K +D R SG + G I Sbjct: 473 KVKAGDVVIVRYEGPKGGPGMQE--MLYPTSYIKSKGLGKACALFTDGRFSGGSSGLVIG 530 Query: 492 HVSPEAAVGGPLSLVRTGDIVELDVSERRLELIVSEEILAQRRKETPQHAPSPHVRGYRQ 551 H SPEAA GG + LV GD++++D+ R + L +S + LA+RR + G RQ Sbjct: 531 HASPEAAEGGAIGLVEEGDMIDIDIPNRTINLRISSDDLAKRRTAMEARGDAAWQPGNRQ 590 Query: 552 LYGREVEQAH 561 + QA+ Sbjct: 591 RVVSQALQAY 600 >gi|300310433|ref|YP_003774525.1| 6-phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 623 Score = 151 bits (382), Expect = 1e-37, Method: Compositional matrix adjust. Identities = 150/547 (27%), Positives = 248/547 (45%), Gaps = 51/547 (9%) Query: 42 REDLMEKPIVGILNTWSDINPCNMNLRMLAQKVREGILEAG-------GIPFEIPLMSIS 94 +ED + P + I++ ++D+ + Q +++ EAG G P ++ Sbjct: 63 KED--KAPSLAIVSAYNDMLSAHQPFERFPQIIKDAAREAGAVAQFAGGSPAMCDGVTQG 120 Query: 95 ENLIKPSSFPFRNMLAMDTEECIRGYPLDAVVLMGGCDKTQPGLLMGAAS-ANVPAIALN 153 + ++ S F R+ +AM T + DA + +G CDK PGLL+GA ++PA+ + Sbjct: 121 QAGMELSLFS-RDAIAMATAIALSHNMFDAALYLGVCDKIVPGLLIGALHFGHLPAVFVP 179 Query: 154 SGPGTPRSAIGGPLATANGVWRFVDQLR-AGCLSTESFENFERQTMGSPGHCSEMGTAST 212 +GP T +N + QL AG + E ++ G C+ GTA++ Sbjct: 180 AGPMTS--------GMSNSDKVKIRQLYTAGKIGRAELLEAESKSYHGAGTCTFYGTANS 231 Query: 213 MSSISEALGLALPGSSLI-PSSDGRRSQIAKAVGRCSVALALSGDLAP-ANILDARAFDN 270 + EA+GL LPG++ I P++ R + A A + + L P ++D ++ N Sbjct: 232 NQMLMEAMGLHLPGAAFITPNTPLRDALTAAAAKQAAKITDLGTQYTPVGRMIDEKSIVN 291 Query: 271 AITLLCAVGGSTNAIIHLLALAGRVGVPLTLDRFDEIARRVPLISNVQPAGEHLTERLMN 330 AI L A GGSTN +HL+A+A G+ + D FD+++ VPLI+ + P G Sbjct: 292 AIVALHATGGSTNHTLHLVAIAKAAGIVIDWDDFDKLSAVVPLITKIYPNGTADVNHFHA 351 Query: 331 AGGVPALLHVL--RPLLHLDARTVDGRTIGE------IADQAEVSDTDVIRQLDTPVKPG 382 AGG +L L L+H D T+ G+ + + +A + E ++ D P + G Sbjct: 352 AGGTGFVLRELIDAGLMHDDVTTILGQGLRQHCKEPMLAAEGENGRPGIVTWHDAPAQSG 411 Query: 383 PS---------------LVVVKGNLAPHGAVIKSCAASPALFKHRGAAVVFEDVQTLADT 427 L +++GNL AVIK A P AVVF + Sbjct: 412 DEGVLGTVAKPFAADGGLKLLQGNLGR--AVIKISAVKPEHRVVEAPAVVFHSQEAFMAA 469 Query: 428 IDDPTMNIDANSVLILRNVGPVGAAMPEWGMLPLPRRLMEHGVKDMLRISDARMSGTAYG 487 ++ D +V+ + GP MPE L +++ + + ++D RMSG + Sbjct: 470 FKAGQLDRDFVAVITFQ--GPRANGMPELHALTPALGILQDKGRHVALVTDGRMSGASGK 527 Query: 488 T-SILHVSPEAAVGGPLSLVRTGDIVELDVSERRLELIVSEEILAQRRKETPQHAPSPHV 546 + +HV+PE GGPL VR GDI+ LD LE V A R +++ + + H Sbjct: 528 VPAAIHVTPEVLGGGPLGRVRDGDIIRLDAEAGTLEAKVDATEWAARSQDSSDLSGNQHG 587 Query: 547 RGYRQLY 553 G R+L+ Sbjct: 588 MG-RELF 593 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.136 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,932,217 Number of extensions: 84505 Number of successful extensions: 293 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 273 Number of HSP's successfully gapped: 6 Length of query: 589 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 498 Effective length of database: 1,188,985 Effective search space: 592114530 Effective search space used: 592114530 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)