BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3503.p (322 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309629|ref|YP_003773721.1| threonine dehydratase [Herbaspi... 184 7e-48 gi|300313197|ref|YP_003777289.1| threonine dehydratase [Herbaspi... 169 3e-43 gi|300309398|ref|YP_003773490.1| threonine dehydratase [Herbaspi... 160 2e-40 gi|300311135|ref|YP_003775227.1| threonine dehydratase [Herbaspi... 107 1e-24 >gi|300309629|ref|YP_003773721.1| threonine dehydratase [Herbaspirillum seropedicae SmR1] Length = 326 Score = 184 bits (468), Expect = 7e-48, Method: Compositional matrix adjust. Identities = 117/317 (36%), Positives = 176/317 (55%), Gaps = 1/317 (0%) Query: 5 EHPTLEDIQKARERLKPHIKHTPLLRAEKIEKAVGCQLYLKPEVLQITGAFKIRGALNKA 64 E P+ D+ A ER+KP TP+LR+ + +G QL+ K E Q GAFK RGA+N Sbjct: 9 ELPSFADVLLAAERIKPWAHRTPVLRSRTADTELGAQLFFKCENFQRMGAFKFRGAMNAL 68 Query: 65 LSLSRDEIANGLIATSSGNHAQGIAYAAKMLGVKATIVVPVTTPKIKIENTQALGAEVIL 124 + +G+I SSGNHAQG A AA++LG+ A IV+P P K+ T+ GAEV+ Sbjct: 69 TQFDATQRKHGVITFSSGNHAQGTALAAQLLGIPAVIVMPQDAPAAKVAATRGYGAEVVT 128 Query: 125 FDGDTAARWEKVCDIAEENKYMAVHGFEDPMVMAGQGTIGCEILEDLEDVDTIIIPLGGG 184 +D T R +A E K + ++ P VMAGQGT E+L++ +D + + LGGG Sbjct: 129 YDRYTEDREAIGARLAAERKMTLIPPYDHPHVMAGQGTATLELLQETGPLDALFVCLGGG 188 Query: 185 GLISGIATAIKETNPSVRVIGAEPALTPKYYQSRINQERISLPLKNTIADGVRISVPGQN 244 GL+SG A A + +P+ ++ G EP QS + + + TIADG + G+ Sbjct: 189 GLLSGAALAARALSPACKIYGVEPQAGNDGQQSLRAGQVVHIDTPKTIADGAQTQHLGKY 248 Query: 245 PFPIIEKYVDEIVLVEDEHIIAGMRALAKDAKLIAEPAASIGLGALLAGSVKVRPDEKVC 304 F +I + VD+I V DE ++AGMR A+ K++ EP +G A +++ ++V Sbjct: 249 TFGVIRELVDDIHTVSDEELVAGMRFFAERMKMVVEPTGCLGFAAARKLKDQLQ-GKRVG 307 Query: 305 LVLSGGNWNLSDLADIF 321 +++SGGN +L LA Sbjct: 308 VIISGGNVDLGKLATFL 324 >gi|300313197|ref|YP_003777289.1| threonine dehydratase [Herbaspirillum seropedicae SmR1] Length = 508 Score = 169 bits (428), Expect = 3e-43, Method: Compositional matrix adjust. Identities = 101/301 (33%), Positives = 168/301 (55%), Gaps = 7/301 (2%) Query: 24 KHTPLLRAEKIEKAVGCQLYLKPEVLQITGAFKIRGALNKALSLSRDEIANGLIATSSGN 83 + TPL A + + + ++Y K E +Q +FK+RGA NK L+ ++ G+I S+GN Sbjct: 19 QETPLELANTLSQRIDNRIYFKREDMQSVFSFKLRGAYNKMAHLTPAQLKRGVICASAGN 78 Query: 84 HAQGIAYAAKMLGVKATIVVPVTTPKIKIENTQALGAEVILFDGDTAARWEKVCDIAEEN 143 HAQG+A +A LG +A IV+P TTP++KI+ +A G EV+LF +E + ++ Sbjct: 79 HAQGVALSAARLGCRAVIVMPTTTPQVKIDAVKARGGEVVLFGDSFTDAYEHALTLEKKQ 138 Query: 144 KYMAVHGFEDPMVMAGQGTIGCEILEDLED-VDTIIIPLGGGGLISGIATAIKETNPSVR 202 K VH F+DP V+AGQGT+G EIL D + I + +GGGGLI+G+A+ +K P ++ Sbjct: 139 KLTFVHPFDDPYVIAGQGTVGMEILRQHPDPIHAIFVAIGGGGLIAGVASYVKAVRPDIK 198 Query: 203 VIGAEPALTPKYYQSRINQERISLPLKNTIADGVRISVPGQNPFPIIEKYVDEIVLVEDE 262 +IG + + +S R++LP +DG + + G+ F I ++ VDE+++V+ + Sbjct: 199 IIGVQTTDSDAMARSLKAGRRVALPDVGLFSDGTAVKLVGEETFRIAKELVDEVIIVDTD 258 Query: 263 HIIAGMRALAKDAKLIAEPAASIGLGALLA------GSVKVRPDEKVCLVLSGGNWNLSD 316 + ++ + +D + I EPA ++ + A S K E + + G N N Sbjct: 259 AVCTAIKDIFQDTRSIVEPAGALAVAGAKAYVERAKASKKPIKGESLITIACGANMNFDR 318 Query: 317 L 317 L Sbjct: 319 L 319 >gi|300309398|ref|YP_003773490.1| threonine dehydratase [Herbaspirillum seropedicae SmR1] Length = 401 Score = 160 bits (404), Expect = 2e-40, Method: Compositional matrix adjust. Identities = 118/310 (38%), Positives = 185/310 (59%), Gaps = 10/310 (3%) Query: 8 TLEDIQKARERLKPHIKHTPLLRAEKIEKAVGCQLYLKPEVLQITGAFKIRGALNKALSL 67 TL DI+ A RL+ I TP + + + + VG Q++LK E LQ T +FK RGA NK + + Sbjct: 3 TLNDIEAAARRLQGQILETPCVESRTLSQIVGAQVFLKFENLQFTASFKERGACNKLVDV 62 Query: 68 SRDEI-ANGLIATSSGNHAQGIAYAAKMLGVKATIVVPVTTPKIKIENTQALGAEVILFD 126 + A G++A S+GNHAQG+AY A+ LG+ ATIV+P TP +KIE T+ GA V+L Sbjct: 63 AASGGGARGVVAMSAGNHAQGVAYHAQRLGLPATIVMPRFTPGVKIERTRGFGANVVL-H 121 Query: 127 GDT--AARWEKVCDIAEENKYMAVHGFEDPMVMAGQGTIGCEILEDLEDVDTIIIPLGGG 184 GDT AAR ++AE+ VH ++D ++AGQGT+ E+++ + D+D +++ +GGG Sbjct: 122 GDTLEAARTH-AYELAEKEGLTFVHPYDDEAIIAGQGTVALEMMKAVPDLDCLVVSIGGG 180 Query: 185 GLISGIATAIKETNPSVRVIGAEPALTPKYYQSRINQERISLPL-KNTIADGVRISVPGQ 243 GL++G+A A + P + ++G + P + E P+ +TIA+G+ + PG Sbjct: 181 GLLAGMAVAARAIKPEIEIVGVQTRRFPTMVNAVKGTEH---PVGASTIAEGIAVGTPGT 237 Query: 244 NPFPIIEKYVDEIVLVEDEHIIAGMRALAKDAKLIAEPAASIGLGALLAGSVKVRPDEKV 303 II + DE++LV++ I + L + K + E A + GL AL+ + R ++V Sbjct: 238 LTRAIIAREADELLLVDEGDIEQAVLMLLEIEKTLVEGAGAAGLAALVRYPDRFR-GKRV 296 Query: 304 CLVLSGGNWN 313 LVLSGGN + Sbjct: 297 GLVLSGGNID 306 >gi|300311135|ref|YP_003775227.1| threonine dehydratase [Herbaspirillum seropedicae SmR1] Length = 351 Score = 107 bits (267), Expect = 1e-24, Method: Compositional matrix adjust. Identities = 77/264 (29%), Positives = 128/264 (48%), Gaps = 2/264 (0%) Query: 7 PTLEDIQKARERLKPHIKHTPLLRAEKIEKAVGCQLYLKPEVLQITGAFKIRGALNKALS 66 P+L DI++A + + T L + +A+G ++++K E TGAFK+RG + Sbjct: 10 PSLTDIEQAAGIVYAGMAPTAQLCWPLLNQALGAEVWVKHENHAPTGAFKVRGGMVYLHH 69 Query: 67 LSRDEIA-NGLIATSSGNHAQGIAYAAKMLGVKATIVVPVTTPKIKIENTQALGAEVILF 125 L+R + +G+++ + GNH Q + AA+ LG+ ATIVVP + K +ALG E+I Sbjct: 70 LARQQPQLSGVVSATRGNHGQSVGLAARRLGISATIVVPQGNSREKNAAMRALGVELIEH 129 Query: 126 DGDTAARWEKVCDIAEENKYMAVHGFEDPMVMAGQGTIGCEILEDLEDVDTIIIPLGGGG 185 + E +A + + + +V AG + E+ +D +++P+G G Sbjct: 130 GSEFQESREYAQQLAAQQQLHMIPSLHRDLV-AGVASYWMELFAAQPGLDVVLVPIGQGS 188 Query: 186 LISGIATAIKETNPSVRVIGAEPALTPKYYQSRINQERISLPLKNTIADGVRISVPGQNP 245 + G A S R+IG A Y S +I P+ IADGV VP Q Sbjct: 189 GMCGAVAARNALGLSTRIIGVVSAHALAYKLSFEAGRKIESPVSTCIADGVACRVPDQAS 248 Query: 246 FPIIEKYVDEIVLVEDEHIIAGMR 269 ++ VDE+V V D+ ++ M+ Sbjct: 249 LEVLFSEVDEVVAVTDDEVMDAMK 272 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.136 0.387 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 892,087 Number of extensions: 32721 Number of successful extensions: 111 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 107 Number of HSP's successfully gapped: 4 Length of query: 322 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 237 Effective length of database: 1,217,395 Effective search space: 288522615 Effective search space used: 288522615 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 130 (54.7 bits)