BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3489.p (393 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312878|ref|YP_003776970.1| aspartate/tyrosine/aromatic ami... 733 0.0 gi|300311875|ref|YP_003775967.1| aspartate/tyrosine/aromatic ami... 132 5e-32 gi|300312316|ref|YP_003776408.1| pyridoxal phosphate dependent a... 128 8e-31 gi|300311409|ref|YP_003775501.1| aspartate aminotransferase [Her... 107 2e-24 gi|300311478|ref|YP_003775570.1| succinyldiaminopimelate aminotr... 79 4e-16 >gi|300312878|ref|YP_003776970.1| aspartate/tyrosine/aromatic aminotransferase [Herbaspirillum seropedicae SmR1] Length = 393 Score = 733 bits (1893), Expect = 0.0, Method: Compositional matrix adjust. Identities = 368/393 (93%), Positives = 380/393 (96%) Query: 1 MNLNQLASRLQHIAPFHVMELSKKADILAQQGRDLIHMGIGEPDFTAPPAVVEAATRAMA 60 MNLNQLA+RLQHIAPFHVMELSKKA +L QQGR LIHMGIGEPDFTAPPAVVEAATRAM Sbjct: 1 MNLNQLAARLQHIAPFHVMELSKKAAVLEQQGRHLIHMGIGEPDFTAPPAVVEAATRAMT 60 Query: 61 EGKMQYTSATGLPALRQAISDHYRSVYGLEIAPERIVITAGASAALLLACAALVERDSEV 120 EGKMQYTSATGLP LR AISDHYRSVYGLEIAPERIV+TAGASAALLLACAALVERDSEV Sbjct: 61 EGKMQYTSATGLPQLRAAISDHYRSVYGLEIAPERIVVTAGASAALLLACAALVERDSEV 120 Query: 121 LMPDPSYPCNRHFVAAFEGRAKLIASGPEHRFQLSAQMVREHWGERTRGVLLASPSNPTG 180 LMPDPSYPCNRHFVAAFEGRAKLIASGPEHRFQLSAQMVREHWG TRGVLLASPSNPTG Sbjct: 121 LMPDPSYPCNRHFVAAFEGRAKLIASGPEHRFQLSAQMVREHWGSATRGVLLASPSNPTG 180 Query: 181 TSIAPDELRAILGEVRQRGGFTIVDEIYQGLSYEGAPFSALSLGEDVVVINSFSKYFNMT 240 TSI PDELRAI+GEVRQRGGFTIVDEIYQGLSYEGAPFSALSLG+DVVVINSFSKYFNMT Sbjct: 181 TSILPDELRAIVGEVRQRGGFTIVDEIYQGLSYEGAPFSALSLGDDVVVINSFSKYFNMT 240 Query: 241 GWRLGWLVLPPALVPQVEKLAQNLLICASSIAQHAAVACFTPETIAVYEARKAEFKRRRD 300 GWRLGWLVLPPALVPQVEKLAQNLLICASSIAQHAAVACFTP+T+A+YE RKAEFKRRRD Sbjct: 241 GWRLGWLVLPPALVPQVEKLAQNLLICASSIAQHAAVACFTPQTLALYEERKAEFKRRRD 300 Query: 301 YIVPALESLGFSVPVVPDGAFYVYADCSALCDDADQLSLDMLNEAGVVLVPGLDFGPFTA 360 YIVPALESLGF+VPV+PDGAFYVYADCSAL DDADQLSLDMLNEAGVVLVPGLDFGPFTA Sbjct: 301 YIVPALESLGFTVPVMPDGAFYVYADCSALADDADQLSLDMLNEAGVVLVPGLDFGPFTA 360 Query: 361 RRYIRLSYATSMEKLQEAVARLRVFFAERKKAA 393 RRYIRLSYATSME LQEAVARLRVFF +R+KAA Sbjct: 361 RRYIRLSYATSMENLQEAVARLRVFFEKRRKAA 393 >gi|300311875|ref|YP_003775967.1| aspartate/tyrosine/aromatic aminotransferase [Herbaspirillum seropedicae SmR1] Length = 431 Score = 132 bits (332), Expect = 5e-32, Method: Compositional matrix adjust. Identities = 107/396 (27%), Positives = 194/396 (48%), Gaps = 23/396 (5%) Query: 9 RLQHIAPFHVMELSKKADILAQQ-GRDLIHMGIGEPDFTAPPAVVE----AATRAMAEGK 63 R++ + P+ V ++ + + A++ G D+I M +G PD PP +VE A R G Sbjct: 43 RIERLPPY-VFNITAELKMAARRRGEDIIDMSMGNPDGATPPHIVEKLVEVAQRPDTHG- 100 Query: 64 MQYTSATGLPALRQAISDHYRSVYGLEIAPE-RIVITAGASAALLLACAALVERDSEVLM 122 Y+S+ G+P LR+AI+ YRS Y ++ P+ ++T G+ L A +++ VL+ Sbjct: 101 --YSSSKGIPRLRRAIAHWYRSRYEVDFDPDSEAIVTIGSKEGLAHLMLATLDKGDTVLV 158 Query: 123 PDPSYPCNRH--FVAAFEGRAKLIASGPEHRFQLSAQMVREHWGERTRGVLLASPSNPTG 180 P+PSYP + + +A R+ ++ G + F + VRE + + + ++L PSNPT Sbjct: 159 PNPSYPIHIYGAVIAGANIRSVRMSPGVDF-FDELERAVRESY-PKPKMMILGFPSNPTA 216 Query: 181 TSIAPDELRAILGEVRQRGGFTIVDEIYQGLSYEG--APFSALSLGEDVVVINSF--SKY 236 + + ++ R+ + D Y ++++G AP G V + F SK Sbjct: 217 QCVELEFFERVVKLAREHQILVVHDLAYADITFDGWKAPSIMQVPGAREVAVEFFTLSKS 276 Query: 237 FNMTGWRLGWLVLPPALVPQVEKLAQNLLICASSIAQHAAVACFTPETIAVYEARKAEFK 296 +NM GWR+G++V LV + ++ + + Q AA+A + V E R A ++ Sbjct: 277 YNMAGWRIGFMVGNARLVAALARIKSYHDYGSFTPVQVAAIAALEGDQSCVEEIR-ANYE 335 Query: 297 RRRDYIVPALESLGFSVPVVPDGAFYVYADCSALCDDADQLSLD--MLNEAGVVLVPGLD 354 RRR+ +V L G+ V VP + Y++A L +L +A V + PG+ Sbjct: 336 RRRNVLVKGLHEAGWMVD-VPKASMYIWARIPEPYRQFGSLEFARILLEQAKVCVSPGIG 394 Query: 355 FGPFTARRYIRLSYATSMEKLQEAVARLRVFFAERK 390 FG + Y+R + + ++++A+ ++ F K Sbjct: 395 FGEY-GDEYVRFALIENESRIRQAIRGIKAMFKNAK 429 >gi|300312316|ref|YP_003776408.1| pyridoxal phosphate dependent aspartate/tyrosine/aromatic aminotransferase [Herbaspirillum seropedicae SmR1] Length = 385 Score = 128 bits (322), Expect = 8e-31, Method: Compositional matrix adjust. Identities = 105/370 (28%), Positives = 176/370 (47%), Gaps = 35/370 (9%) Query: 33 RDLIHMGIGEPDFTAPPAVVEAATRAMAEGKMQYTSATGLPALRQAISDHYRSVYGLEIA 92 + +++G G PDF PA+V A T+AM EG QY G+P LR+AI++ +YG Sbjct: 28 KGAVNLGQGFPDFHCDPALVAAVTQAMQEGLNQYPPMAGVPVLREAIAEKVEKLYGHRYD 87 Query: 93 P-ERIVITAGASAALLLACAALVERDSEVLMPDPSYPCNRHFVAAFEGRAKLIASGPEH- 150 P I +TAGA+ +L + V EV++ +P Y C +V A E L P Sbjct: 88 PVSEITVTAGATQGILTSVLCAVHPGDEVIVIEPVYDC---YVPAIE----LAGGVPVFV 140 Query: 151 RFQLSAQMVREHWGE-------RTRGVLLASPSNPTGTSIAPDELRAILGEVRQRGGFTI 203 + ++ AQ W + +TR +++ +P NPTG+ + ++ A+ VR + Sbjct: 141 QMEVGAQGYSIPWDKVKAAVTPKTRMIMVNTPHNPTGSVMRAADVAALADIVRGTDILIL 200 Query: 204 VDEIYQGLSYEGAPFSALS----LGEDVVVINSFSKYFNMTGWRLGWLVLPPALVPQVEK 259 DE+Y+ + Y+G P +L+ L E + +SF K +++TGW++G++ P AL + K Sbjct: 201 SDEVYEHMVYDGQPHESLARHPELAERSFINSSFGKTYHVTGWKVGYVAAPAALTAEFRK 260 Query: 260 LAQNLLICASSIAQHAAVACFTPETIAVYEARKAEFKRRRDYIVPALESLGFSVPVVPDG 319 + Q + ++ Q+ A + A Y A ++ +RD L + F ++P Sbjct: 261 VHQFNVFTVNTPVQYGLAAYM--KDPAPYLDLPAFYQHKRDLFRTGLANTRFE--LLPSQ 316 Query: 320 AFYV----YADCSALCDDADQLSLDMLNEAGVVLVPGLDFGPFTARR---YIRLSYATSM 372 Y Y SAL + + + +E GV +P F + R +R +A Sbjct: 317 GTYFQCVKYGAISALPE--AEFCKWLTSEIGVAAIPVSAF--YNTPRESGIVRFCFAKKD 372 Query: 373 EKLQEAVARL 382 E LQ A+ RL Sbjct: 373 ETLQLALERL 382 >gi|300311409|ref|YP_003775501.1| aspartate aminotransferase [Herbaspirillum seropedicae SmR1] Length = 409 Score = 107 bits (266), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 96/382 (25%), Positives = 176/382 (46%), Gaps = 23/382 (6%) Query: 23 KKADILAQQGRDLIHMGIGEP---DFTAPPAVVEAATRAMAEGKMQYTSATGLPALRQAI 79 +KA + ++G +I + IG F AP +V+ R M YT + GL A R+++ Sbjct: 23 EKARQMEEEGHKIIKLNIGNLAAFGFDAPDEIVQDMIRNMGNAS-GYTDSKGLFAPRKSV 81 Query: 80 SDHYRSVYGLE-IAPERIVITAGASAALLLACAALVERDSEVLMPDPSYPCNRHFVAAFE 138 HY +E + + I + GAS ++++ AL+ EVL+P P YP V+ Sbjct: 82 M-HYTQQKKIEGVTIDDIYLGNGASELIVMSVNALLNTGDEVLVPSPDYPLWTAAVSLSG 140 Query: 139 GRAKLIASGPEHRFQLSAQMVREHWGERTRGVLLASPSNPTGTSIAPDELRAILGEVRQR 198 G + +Q +++ T+ +++ +P+NPTG + + L+ I+ RQ Sbjct: 141 GTPVHYVCDEQQGWQPDIADIKKKITPNTKAIVVINPNNPTGALYSVEVLKEIIELARQH 200 Query: 199 GGFTIVDEIYQGLSYEGAPFSAL-SLGEDVVVI--NSFSKYFNMTGWRLGWLVLPPA--- 252 + DEIY + Y+G ++L SL +DV+ I N SK + G+R GW+V+ Sbjct: 201 QLIILADEIYDKVLYDGNTHTSLASLADDVLFITFNGLSKNYRSCGYRAGWMVVSGEKKH 260 Query: 253 ---LVPQVEKLAQNLLICASSIAQHA-AVACFTPETIAVYEARKAEFKRRRDYIVPALES 308 + + LA ++ +CA++ Q A A ++I + ++RD L Sbjct: 261 ARDYIEGLNMLA-SMRLCANAPGQFAIQTALGGYQSINDLVGPQGRLTKQRDLAHQLLTD 319 Query: 309 LGFSVPVVPDGAFYVY----ADCSALCDDADQLSLDMLNEAGVVLVPGLDFGPFTARRYI 364 + V P A Y++ + + DD + + ++L E V++V G F T + Sbjct: 320 IPGVSCVKPKSALYMFPRLDPEIYPIKDDQ-EFAYELLAEEKVLIVQGTGFNCPTPDHF- 377 Query: 365 RLSYATSMEKLQEAVARLRVFF 386 R+ + + + L E++ R+ F Sbjct: 378 RVVFLPNTDDLTESMGRIAHFL 399 >gi|300311478|ref|YP_003775570.1| succinyldiaminopimelate aminotransferase [Herbaspirillum seropedicae SmR1] Length = 420 Score = 79.3 bits (194), Expect = 4e-16, Method: Compositional matrix adjust. Identities = 104/409 (25%), Positives = 170/409 (41%), Gaps = 51/409 (12%) Query: 10 LQHIAPFHVMELSKK-ADILAQQGRDLIHMGIGEPDFTAPPAVVEAATRAMAEGKMQYTS 68 L + P+ +L K A I I +GIGEP P + +A +A G Y + Sbjct: 22 LDQLQPYPFEKLKKLFAGITPDPAYRPISLGIGEPKHPTPAFIQQALIDNLA-GLASYPA 80 Query: 69 ATGLPALRQAISDHYRSVYGL---EIAPERIVITAGASAALLLACAALVERDSEVLMPDP 125 G ALR AI+D + Y + + A E I+ G+ AL +V DP Sbjct: 81 TLGSDALRGAIADWLQKRYDIPRPDPATE-ILPVNGSREALFSLTQTVV---------DP 130 Query: 126 SYP-----CNRHFVAAFEGRAKL-------IASGPEHRFQLSAQMVREHWGERTRGVLLA 173 + P C F +EG A L + S P+ F V + ER + + + Sbjct: 131 TRPGALVMCPNPFYQIYEGSAYLAGAQPYFVNSDPKRNFAPDFSQVPQDVWERVQLLFVC 190 Query: 174 SPSNPTGTSIAPDELRAILGEVRQRGGFTIV-DEIYQGLSY-EGAPFSALS----LGED- 226 SP NPTG + ++ + + + R GF I DE Y + + E P + LG D Sbjct: 191 SPGNPTGAVLTLEDWKELFA-LSDRYGFVIASDECYSEIYFNEDPPLGGMQAARLLGRDY 249 Query: 227 --VVVINSFSKYFNMTGWRLGWLVLPPALVPQVEKLAQNLLICASSIAQHAAVACFTPET 284 ++ +S SK N+ G R G++ A++ + S + Q A+V + ET Sbjct: 250 RRLISFSSLSKRSNVPGMRSGFVAGDAAILKKFLLYRTYHGAAMSPVIQAASVLAWQDET 309 Query: 285 IAVYEARKAEFKRRRDYIVPALESLGFSVPVVPDGAFYVYADCSALCDDAD-QLSLDMLN 343 + A++ + + P L+ + V +PD AFY++A L + +D + + + Sbjct: 310 HV--QGNIAKYVTKFSQVTPLLQEV-LEV-ALPDAAFYLWAKVDKLVNISDTEFAQRLYA 365 Query: 344 EAGVVLVPGL-------DFGPFTARRYIRLSYATSMEKLQEAVARLRVF 385 E V ++PG P R IR++ +E+ EA R+ F Sbjct: 366 EYNVTVLPGSYLAREAHGVNPGAGR--IRMALVAEVEECLEAAQRIVAF 412 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.135 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,196,304 Number of extensions: 49999 Number of successful extensions: 190 Number of sequences better than 1.0e-08: 8 Number of HSP's gapped: 181 Number of HSP's successfully gapped: 8 Length of query: 393 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 306 Effective length of database: 1,207,925 Effective search space: 369625050 Effective search space used: 369625050 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 131 (55.1 bits)