BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3484.p (227 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312873|ref|YP_003776965.1| hypothetical protein Hsero_3578... 432 e-122 gi|300310596|ref|YP_003774688.1| 2-keto-4-pentenoate hydratase/2... 118 5e-28 >gi|300312873|ref|YP_003776965.1| hypothetical protein Hsero_3578 [Herbaspirillum seropedicae SmR1] Length = 227 Score = 432 bits (1111), Expect = e-122, Method: Compositional matrix adjust. Identities = 207/227 (91%), Positives = 219/227 (96%) Query: 1 MSFAIPVPAQTTIPVVGGLPFPVRRIYCVGRNYAAHAREMGYDPDREPPFFFMKPADAIV 60 MSFAIP PAQ T+ VVGG FPVRRIYCVGRNYAAHAREMG+DPDREPPFFFMKPADA+V Sbjct: 1 MSFAIPAPAQATVAVVGGAAFPVRRIYCVGRNYAAHAREMGHDPDREPPFFFMKPADAVV 60 Query: 61 PTDSVVPYPVATADYHHEIEMVVALAKGGRDIPVEQALELVYGYAVGLDMTRRDLQAQAK 120 PTDSVVPYPVAT+DYHHEIE+VVALAKGGR+IPVEQALELV+GYAVGLDMTRRD+Q+QAK Sbjct: 61 PTDSVVPYPVATSDYHHEIELVVALAKGGRNIPVEQALELVFGYAVGLDMTRRDIQSQAK 120 Query: 121 KMGRPWDMGKGFDHSAPCAPIVPVAQAGHPDQGAVWLKVNGEVRQQGDLADLIWNVAETI 180 K+GRPWDM KGFD SAPCAPIVPVAQAGHPDQGAVWLKVNGEVRQQGDLADLIW+VAETI Sbjct: 121 KLGRPWDMAKGFDQSAPCAPIVPVAQAGHPDQGAVWLKVNGEVRQQGDLADLIWSVAETI 180 Query: 181 SYLSSLVELFPGDLIFTGTPEGVGAVVKGDQLQGHVDGLPDINITIG 227 SYLS LVELFPGDLIFTGTPEGVGAVVKGD+LQGHVDGLPDINITIG Sbjct: 181 SYLSGLVELFPGDLIFTGTPEGVGAVVKGDKLQGHVDGLPDINITIG 227 >gi|300310596|ref|YP_003774688.1| 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase [Herbaspirillum seropedicae SmR1] Length = 281 Score = 118 bits (295), Expect = 5e-28, Method: Compositional matrix adjust. Identities = 80/224 (35%), Positives = 113/224 (50%), Gaps = 19/224 (8%) Query: 11 TTIPVVGGLPF------PVRRIYCVGRNYAAHAREMGYDPDREPPFFFMKPADAIVPTDS 64 T+PVV G P + + C+G NYA HA E P P F K A+V + Sbjct: 52 NTLPVVEGNPRIGACVGNIGKFICIGLNYADHAAESNL-PIPAEPVVFNKWTSAVVGPND 110 Query: 65 VVPYPVATADYHHEIEMVVALAKGGRDIPVEQALELVYGYAVGLDMTRRDLQAQAKKMGR 124 V P + E+E+ V + KGG I + A+ V GY V D++ R+ Q + G Sbjct: 111 NVKIPRGSKKTDWEVELGVIIGKGGSYIDEKDAMSHVAGYCVVNDVSEREYQIE---RGG 167 Query: 125 PWDMGKGFDHSAPCAP-IVPVAQAGHPDQGAVWLKVNGEVRQQGDLADLIWNVAETISYL 183 WD GKG D P P +V + P + +WL+V+G+ Q G+ + +I+NVA +SYL Sbjct: 168 TWDKGKGCDTFGPIGPWLVTRDEVADPQKLGMWLEVDGKRYQNGNTSTMIFNVAHIVSYL 227 Query: 184 SSLVELFPGDLIFTGTPEGVGAVVK--------GDQLQGHVDGL 219 S + L PGD+I TGTP GVG VK G ++ +DGL Sbjct: 228 SRFMSLQPGDVISTGTPPGVGMGVKPEAVYLRAGQTIRLGIDGL 271 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.140 0.438 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 868,301 Number of extensions: 40887 Number of successful extensions: 91 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 88 Number of HSP's successfully gapped: 2 Length of query: 227 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 145 Effective length of database: 1,231,600 Effective search space: 178582000 Effective search space used: 178582000 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)