BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3458.p (324 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312848|ref|YP_003776940.1| NADPH:quinone oxidoreductase [H... 616 e-178 gi|300311093|ref|YP_003775185.1| NADPH:quinone oxidoreductase [H... 135 4e-33 gi|300310297|ref|YP_003774389.1| alcohol dehydrogenase [Herbaspi... 99 4e-22 gi|300312794|ref|YP_003776886.1| NADPH:quinone oxidoreductase [H... 92 7e-20 gi|300310813|ref|YP_003774905.1| NADPH:quinone oxidoreductase [H... 84 2e-17 >gi|300312848|ref|YP_003776940.1| NADPH:quinone oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 324 Score = 616 bits (1589), Expect = e-178, Method: Compositional matrix adjust. Identities = 300/324 (92%), Positives = 311/324 (95%) Query: 1 MAKAIRMNKTGGPEVMEYVDVEVGEPGAGQVRIRHAAIGLNFIDCYFRAGLYPQPLPAGL 60 MAKAIRM+KTGGPEVMEYVDVEVGEPGAG+VRIRHAA+GLNFIDCYFRAGLYPQPLPAGL Sbjct: 1 MAKAIRMSKTGGPEVMEYVDVEVGEPGAGEVRIRHAAVGLNFIDCYFRAGLYPQPLPAGL 60 Query: 61 GQEGAGTIEAVGPGVSDFKVGDRVAYAGRPNGAYAEERVMPADILVKLPDAISFDTAAAM 120 GQE AGTIEAVG GVS FKVGDRVAYAGRPNGAYAEERVMPADILVKLPDAISFDTAAAM Sbjct: 61 GQEAAGTIEAVGAGVSGFKVGDRVAYAGRPNGAYAEERVMPADILVKLPDAISFDTAAAM 120 Query: 121 MLQGMTVQYLLRQTYVVQPGETILFHAAAGGIGLIACQWARALGVNLIGTVSSEEKAALA 180 MLQGMTVQYLLRQTY VQPG+TILFHAAAGGIGLIACQWARALGVNLIGTVSS+EKAALA Sbjct: 121 MLQGMTVQYLLRQTYAVQPGDTILFHAAAGGIGLIACQWARALGVNLIGTVSSDEKAALA 180 Query: 181 KQHGAAHVINYKAENFVERVKEITGGKGVPVVYDSIGKDTFMGSLDCLRPRGMMVSFGNA 240 QHGAAHVINYK ENFVERVKE+TGG+GV VVYDSIGKDTF+GSLDCLRPRGMMVSFGNA Sbjct: 181 LQHGAAHVINYKTENFVERVKELTGGRGVQVVYDSIGKDTFLGSLDCLRPRGMMVSFGNA 240 Query: 241 SGPVAPFGVSELAARGSLFLTRPSLGNYIATRAELDATAGDLFHMVQSKQISIEINQRYA 300 SGPVAPFGVSELA+RGSLFLTRPSLGNY +TRAELDATAGDLF MV +ISIEINQRYA Sbjct: 241 SGPVAPFGVSELASRGSLFLTRPSLGNYTSTRAELDATAGDLFDMVLGNKISIEINQRYA 300 Query: 301 LKDVGQAHADLEGRKTTGSTILIP 324 LKDV QAHADLEGRKTTGSTILIP Sbjct: 301 LKDVAQAHADLEGRKTTGSTILIP 324 >gi|300311093|ref|YP_003775185.1| NADPH:quinone oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 325 Score = 135 bits (341), Expect = 4e-33, Method: Compositional matrix adjust. Identities = 111/331 (33%), Positives = 160/331 (48%), Gaps = 20/331 (6%) Query: 3 KAIRMNKTGGPEVMEYVDVEVGEPGAGQVRIRHAAIGLNFIDCYFRAGLYPQP-----LP 57 +AI + + GGPEV++ + V E AG+V I+ A G+N D + R G YP P LP Sbjct: 2 RAIEIKEFGGPEVLQACERPVPELKAGEVLIKVHAAGVNRPDVFQRTGNYPVPPGASDLP 61 Query: 58 AGLGQEGAGTIEAVGPGVSDFKVGDRVAYAGRPNGAYAEERVMPADILVKLPDAISFDTA 117 G E AG I A G S FK+GD V A G YAE P + +P +S A Sbjct: 62 ---GLEVAGEIVAGDLGDSGFKLGDLVC-ALVQGGGYAEFCAAPLAQCLPVPKGLSALEA 117 Query: 118 AAMMLQGMTVQYLLRQTYVVQPGETILFHAAAGGIGLIACQWARALGVNLIGTVSSEEKA 177 AA+ TV + + ET+L + GIG+ A Q ARALG + T S EK Sbjct: 118 AALPENYFTVWSNVFDRGQLSGEETLLVQGGSSGIGVTAIQIARALGHRVFATAGSAEKC 177 Query: 178 ALAKQHGAAHVINYKAENFVERVKEITGGKGVPVVYDSIGKDTFMGSLDCLRPRGMMVSF 237 ++ GA INYK E+F VKE+TGG+GV V+ D +G D + L G +V Sbjct: 178 RACEELGAERAINYKTEDFEAVVKELTGGRGVDVILDMVGGDYLPREIKALADDGRLVFI 237 Query: 238 GNASGPVAPFGVSELAAR-----GSLFLTRPSLGNYIATRAELDATAGD-LFHMVQSKQI 291 G + ++ R GS RP +A +A + A + ++ ++++ +I Sbjct: 238 AQLGGAKGQLDMGQVMRRRLTITGSTLRPRP-----VAFKAAIAAKLREHVWPLIEAGKI 292 Query: 292 SIEINQRYALKDVGQAHADLEGRKTTGSTIL 322 I+ R+ L+ QAHA +EG G +L Sbjct: 293 RPVIHHRFPLEQAAQAHAMMEGSTHIGKIML 323 >gi|300310297|ref|YP_003774389.1| alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 341 Score = 99.4 bits (246), Expect = 4e-22, Method: Compositional matrix adjust. Identities = 93/310 (30%), Positives = 144/310 (46%), Gaps = 44/310 (14%) Query: 1 MAKAIRMNKTGGPEVMEYVDVEVGEPGAGQVRIRHAAIGLNFIDCYFRAGLYP-QPLPAG 59 M KA + + G P +E V V P GQ+ ++ A G+ D + G +P +P P Sbjct: 4 MMKAAVVREFGKPLSIEQVPVPT--PAPGQILVKFEASGVCHTDLHAAHGDWPVKPTPPF 61 Query: 60 L-GQEGAGTIEAVGPGVSDFKVGDRVAYAGRP---------------------------- 90 + G EG G + AVG GV K GDRV G P Sbjct: 62 IPGHEGTGYVAAVGAGVKHVKEGDRV---GVPWLHTACGCCSPCRTGWETLCAEQQNTGY 118 Query: 91 --NGAYAEERVMPADILVKLPDAISFDTAAAMMLQGMTVQYLLRQTYVVQPGETILFHAA 148 NG++AE + + LPD + F AA ++ G+TV L++T V+PGE ++ + Sbjct: 119 SVNGSFAEYGLADPKFVGHLPDNLDFGPAAPVLCAGVTVYKGLKETE-VRPGEWVVI-SG 176 Query: 149 AGGIGLIACQWARALGVNLIGTVSSEEKAALAKQHGAAHVINYKAENFVERVKEITGGKG 208 GG+G +A Q+A+A+G++++ E+K ALAK+ GA ++ + N V V+ I GG Sbjct: 177 IGGLGHMAVQYAKAMGMHVVAADIHEDKLALAKKLGADLTVDGRNHNAVAEVQRIIGGAH 236 Query: 209 VPVVYDSIGKDTFMGSLDCLRPRGMM----VSFGNASGPVAPFGVSELAARGSLFLTRPS 264 +V ++ + LR RG M + G+ S PV + + RGS+ TR Sbjct: 237 GALV-TAVSPKAMEQAFGFLRARGTMALVGLPPGDISLPVFNTVLKRITVRGSIVGTRQD 295 Query: 265 LGNYIATRAE 274 L + AE Sbjct: 296 LEESLVFAAE 305 >gi|300312794|ref|YP_003776886.1| NADPH:quinone oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 342 Score = 91.7 bits (226), Expect = 7e-20, Method: Compositional matrix adjust. Identities = 106/340 (31%), Positives = 156/340 (45%), Gaps = 58/340 (17%) Query: 3 KAIRMNKTGGPEVMEYVDVEVGEPGAGQVRIRHAAIGLNFIDCYFRAG---LYP--QP-- 55 KAI+ + GGPEV+ Y D V E G+V +R A+GLN D Y R G L P QP Sbjct: 7 KAIQQHAFGGPEVLRYEDAPVPELQPGEVLVRVHAVGLNPPDWYLREGYKMLPPEWQPTV 66 Query: 56 -LPAGLGQEGAGTIEAVGPGVSDFKVGDRVAYAGR-PNG------AYAEERVMPADILVK 107 P LG + +G +EAV V DF VGD V R P+G AYA+ + A + Sbjct: 67 AFPLILGTDISGVVEAVADDVKDFAVGDEVYSMVRFPSGLAGGSRAYAQYVSVAASEIAL 126 Query: 108 LPDAISFDTAAAMMLQGMTV-QYLLRQTYVVQ-------------PGETILFHAAAGGIG 153 P IS AAA + +T Q+++ + +Q +T++ + AAGG+G Sbjct: 127 KPQRISHAQAAAAPMSLLTAWQFMIELGHDIQNPLQPHRHEPVPLKDKTVVVNGAAGGVG 186 Query: 154 LIACQWARALGVNLIGTVSSEEKAALAKQHGAAHVINYK---AENFVERVKEITGGKGVP 210 A Q A+ G +I S +A L + GA I+Y AE+ + V Sbjct: 187 HFALQLAKWKGARVIAIGSGGHEAFL-RDLGADEFIDYTRSAAEDVLSDVD--------- 236 Query: 211 VVYDSIGKDTFMGSLDCLRPRGMMVSFGNASGPVAPFGVS--ELAARGSLFLTRPSLGNY 268 +V D++G L L+ G A P+ P G S E AAR + + S Sbjct: 237 LVIDAVGGPASGRFLRTLK-------RGGALFPIFPLGFSGAEEAARLGVNV---STTQV 286 Query: 269 IATRAELDATAGDLFHMVQSKQISIEINQRYALKDVGQAH 308 ++ A+L AG ++ S + + I+ +AL+D G+AH Sbjct: 287 RSSGAQLQQLAG----LLDSGVVRVAIDSTFALQDAGKAH 322 >gi|300310813|ref|YP_003774905.1| NADPH:quinone oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 309 Score = 83.6 bits (205), Expect = 2e-17, Method: Compositional matrix adjust. Identities = 86/287 (29%), Positives = 130/287 (45%), Gaps = 33/287 (11%) Query: 3 KAIRMNKTGGPEVMEYVDVEVGEPGAGQVRIRHAAIGLNFIDCYFRAG----LYPQPLPA 58 +AI ++ G P V+ D++ G VR+ A G+ +D RAG L LP Sbjct: 2 RAICYHQYGPPSVLHLCDLDEQPLPPGHVRVAMQAAGVAPVDTKLRAGRLQHLVALDLPK 61 Query: 59 GLGQEGAGTIEAVGPGVSDFKVGDRV----AYAGRPNGAYAEERVMPADILVKLPDAISF 114 G++GAG I A+G GV +VG+ V + AGR G E V+P + +V+ PD +S Sbjct: 62 VPGRDGAGVITALGEGVHTLQVGEAVCVMPSMAGR--GTTLAELVLPQEQVVRKPDVLSM 119 Query: 115 DTAAAMMLQGMTVQYLLRQTYVVQPGETILFHAAAGGIGLIACQWARALGVNLIGTVSS- 173 AAA++ + L QT + G + HA +G +G + Q A LG + + S Sbjct: 120 QQAAALLQPACSAWIALMQTAPIAAGMKVFIHAGSGAVGSLMVQLAAHLGAEVTASCRSS 179 Query: 174 --EEKAALAKQHGAAHVINYKAENFVERVKEITGGKGVPVVYDSIGKDTFMGSLDCLRPR 231 E AAL GA+ V+ Y + RV++ VV D +G S LR Sbjct: 180 NVEYVAAL----GASRVLAYDRDEL-GRVRDQD------VVIDLVGGAAHDQSYRMLRRG 228 Query: 232 GMMVSFGNASGPV----APFGVSELAARGSLFLTRPSLGNYIATRAE 274 G +V + P+ FGVS R +L + S+ +A A+ Sbjct: 229 GQLVWL--VAAPIRERGEEFGVS---VRRALIVEDQSILQQVAALAQ 270 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.137 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,107,026 Number of extensions: 51761 Number of successful extensions: 196 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 189 Number of HSP's successfully gapped: 6 Length of query: 324 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 239 Effective length of database: 1,217,395 Effective search space: 290957405 Effective search space used: 290957405 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)