BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3424.p (920 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312757|ref|YP_003776849.1| Zn-dependent peptidase [Herbasp... 1522 0.0 gi|300313431|ref|YP_003777523.1| zinc protease [Herbaspirillum s... 138 2e-33 gi|300313429|ref|YP_003777521.1| zinc protease-like signal pepti... 97 6e-21 >gi|300312757|ref|YP_003776849.1| Zn-dependent peptidase [Herbaspirillum seropedicae SmR1] Length = 929 Score = 1522 bits (3941), Expect = 0.0, Method: Compositional matrix adjust. Identities = 738/923 (79%), Positives = 818/923 (88%), Gaps = 9/923 (0%) Query: 3 ELILSLRHLRLAILSVSFGFILAAPTSQAATSTL---------ALPAGVSAGPSAEGISE 53 E LSLR RLA+L +S GF L+ P +QAAT+T +LPAGVSAGPSAEGISE Sbjct: 3 ESTLSLRCPRLALLVLSLGFFLSVPAAQAATATADAAPPAAIKSLPAGVSAGPSAEGISE 62 Query: 54 YHFANGFKLLLLPDDSKPTVTVNITYLVGSRHENYGETGMAHLLEHLMFKGSPQHPAIPQ 113 Y FANGFKLLLLPDDS+PTVTVNITYLVGSRHENYGETGMAHLLEHLMFKGSP+HP+IPQ Sbjct: 63 YRFANGFKLLLLPDDSQPTVTVNITYLVGSRHENYGETGMAHLLEHLMFKGSPRHPSIPQ 122 Query: 114 EFSKRGMNFNGTTCLDRTNYYESFQAGQDNLQWAIAMEADRMVNSKIARKDLDSEMTVVR 173 +FSKRGMNFNGTT LDRTNYYE+FQA DNL+WAIAMEADRM+NSKIARKDLDSEMTVVR Sbjct: 123 DFSKRGMNFNGTTWLDRTNYYETFQASPDNLRWAIAMEADRMLNSKIARKDLDSEMTVVR 182 Query: 174 NEYEAGETSPARVMLKRMQSVAYDWHSYGRNTIGARSDIENVRIENLQAFYRTYYQPDNA 233 NE+EAGETSP RVMLKRMQSVAYDWH+YGRNTIGARSDIENVRIENLQAFYRTYYQPDNA Sbjct: 183 NEFEAGETSPTRVMLKRMQSVAYDWHAYGRNTIGARSDIENVRIENLQAFYRTYYQPDNA 242 Query: 234 VLIIAGKFEPRQVLQWVNQSFGRLPKPKRKLPVFWTVEPTQDGERQFTIRRRGDLQLVAL 293 VL+IAGKF+ QVLQWV+QSFGRLPKPKR LP FWTVEPTQDGERQF IRRRGD QLVAL Sbjct: 243 VLLIAGKFDAAQVLQWVDQSFGRLPKPKRTLPAFWTVEPTQDGERQFVIRRRGDQQLVAL 302 Query: 294 GYKMPSALHPDATALAFASDILTDTPNGRLHKSLVQTGLATEVYAMPLAGYAPGLQLIVA 353 YKMPSALHPDATAL FA+DILTDTPNGRLHK+LV+TG+ATEVYAMPL G APGLQ++VA Sbjct: 303 AYKMPSALHPDATALGFAADILTDTPNGRLHKALVETGMATEVYAMPLEGMAPGLQMMVA 362 Query: 354 KLKAGADIEPVKQAMIATVESFSSNPPTAEEMARLRRESANNFETLQNNPQQMAVAMSNA 413 K+K G D++ V+QAMI+ VESFS+ PPT EE+ARL RE+AN+FETLQNNPQQ+AVAMSNA Sbjct: 363 KVKVGGDLDAVRQAMISAVESFSTQPPTPEEVARLHREAANSFETLQNNPQQLAVAMSNA 422 Query: 414 IARSDWRLLFIERDLEQRLSSADIATAAGKYFRRDNRTVGLYIPEDHPQRAEIPPAPSVD 473 IAR DWRLLFIERD QRLSSADIA AAG+YFRRDNRTVGLY+P+DHPQRAEIP AP++D Sbjct: 423 IARGDWRLLFIERDRMQRLSSADIAAAAGRYFRRDNRTVGLYLPDDHPQRAEIPTAPALD 482 Query: 474 ALLAQYQPRAPIAAGEAFDPAPGNIEQRTTRIAPGQPPHAALKLALLPKQSRGQTVSVSM 533 LL+QYQPR IA+GEAFDPAP NIEQRTTRIAPGQPPH AL LALLPKQSRGQTVSVS+ Sbjct: 483 TLLSQYQPRTAIASGEAFDPAPANIEQRTTRIAPGQPPHTALDLALLPKQSRGQTVSVSL 542 Query: 534 NLEFGDADSLFGQRAVAQLTAAMLMRGTSTLDRRQLADEFTRLKINGNVYRFQTTRENLN 593 +L FGDA SLFGQR VAQLT AMLMRGT LDRRQLADEF RLKING++YRFQTTRENL Sbjct: 543 HLRFGDAQSLFGQRNVAQLTEAMLMRGTDKLDRRQLADEFARLKINGSLYRFQTTRENLV 602 Query: 594 AAIALAGEVLRHPRMDPAEFAQLKNETVAALEATRKEPDARAAEALQLHFDHYPAGDWRA 653 AIAL GEV RHPRMD AEF QLKNETVA LEA RK+PD RA EAL +HFDHYPAGDWRA Sbjct: 603 PAIALVGEVFRHPRMDAAEFEQLKNETVAGLEAGRKDPDTRAGEALSVHFDHYPAGDWRA 662 Query: 654 ARTLEERIAAVQAVTLEQVQAFHRRFYGASHGEVAIVGDFDAQAARSAIDQAFAGWSSAA 713 A+TLE+RIAAV+AVTLEQV+ FHRRFYGAS GE+A+VGDFD ARSAIDQAF GW+SAA Sbjct: 663 AQTLEQRIAAVRAVTLEQVKDFHRRFYGASQGELAVVGDFDPAQARSAIDQAFGGWTSAA 722 Query: 714 PYARILRTWSDIAPKRILIDTPDKENGVYLARQNLRLRDDDPDYATLVVANYLLGASSLK 773 PYAR+L+TW+DI KR+L+DTPDKENGVY ARQN+RL D DPDY L VAN+LLG SSLK Sbjct: 723 PYARVLQTWADIPAKRLLVDTPDKENGVYFARQNIRLSDTDPDYPALAVANFLLGGSSLK 782 Query: 774 SRLGDRVRQQDGLSYGINSGLQIGAISDAGYLVIHAIAAPQNMDKVDAAVREELDRLLKD 833 SRL DRVRQQDGLSYGI SGLQ+ A++DAG+L I+AIAAPQN+DKVDAAVREE+ LKD Sbjct: 783 SRLADRVRQQDGLSYGIYSGLQVAALTDAGHLTIYAIAAPQNLDKVDAAVREEIALALKD 842 Query: 834 GFSEDELLRAKSGILQQRNQQRSSDGGMATGWTALLNLDRSFLWQQQLDLKLADLTLAQL 893 GFSE+EL RAKSGILQQRNQQR+ + +A WT L+N DR+FLWQQ++D KLA LTLAQ+ Sbjct: 843 GFSEEELQRAKSGILQQRNQQRAGNDNVAATWTLLMNQDRNFLWQQEIDRKLASLTLAQV 902 Query: 894 NAAVRKYIDPARMTVVMARDAAK 916 NAA+RKY+DPARMT V+ARD K Sbjct: 903 NAALRKYVDPARMTTVIARDETK 925 >gi|300313431|ref|YP_003777523.1| zinc protease [Herbaspirillum seropedicae SmR1] Length = 459 Score = 138 bits (347), Expect = 2e-33, Method: Compositional matrix adjust. Identities = 118/440 (26%), Positives = 205/440 (46%), Gaps = 31/440 (7%) Query: 48 AEGISEYHFANGFKLLLLPDDSKPTVTVNITYLVGSRHENYGETGMAHLLEHLMFKGSPQ 107 AE ++ NG K+++ D PT + Y VG E G TG++H LEH+MFKG+ Sbjct: 26 AEPARQFVLGNGMKVIVKEDRRAPTAVQMVWYKVGGIDEVNGLTGVSHALEHMMFKGTRN 85 Query: 108 HPAIPQEFSKR----GMNFNGTTCLDRTNYYESFQAGQDNLQWAIAMEADRMVNSKIARK 163 H EFS+ G N T D T Y++ + + +L+ +A+EADRM N + Sbjct: 86 HKV--GEFSRLVAELGGQENAFTANDFTAYFQQIE--KSHLEKVMALEADRMANLQFDAA 141 Query: 164 DLDSEMTVVRNEYE-AGETSPARVMLKRMQSVAYDWHSYGRNTIGARSDIENVRIENLQA 222 + E+ V+ E + P ++ + + + A+ H Y +G D++++ ++++ A Sbjct: 142 EFAKEIRVIMEERRWRTDDQPMGLLNEALNAAAWTAHPYHHPVVGWMDDLQHMSVQDIAA 201 Query: 223 FYRTYYQPDNAVLIIAGKFEPRQVLQWVNQSFGRLPKPKRKLPVFW-TVEPTQDGERQFT 281 +YR +Y P+NA L++AG + ++VL + FG++ P R+LP EP Q G R+ T Sbjct: 202 WYRQWYAPNNATLVVAGDVDAQRVLALARKYFGKI--PARRLPAGKPQNEPQQLGMRRVT 259 Query: 282 IRRRGDLQLVALGYKMPSALH----PDATALAFASDILTDTPNGRLHKSLVQTGLATEVY 337 ++ + V + +K P+ D AL S +L N RL SLV+TG Sbjct: 260 VKAPAENPYVVMAWKTPALRQVEQDQDVYALDVLSAVLDGYDNARLSASLVRTGGKATAV 319 Query: 338 AMPLAGYAPGLQLIVAKLKAGADIEPVKQAMI-----ATVESFSSNPPTAEEMARLRRE- 391 +G A G L + G+ E V A + VE + + +E+ R++ + Sbjct: 320 GASYSGVARGPVLFTLE---GSPAEGVTTAQLEGLLRGEVERIAREGVSEQELQRVKTQL 376 Query: 392 -SANNFETLQNNPQQMAVAMSNAIARSDWRLLFIERDLE--QRLSSADIATAAGKYFRRD 448 ++ ++ Q M + M I+R +E + +++A + A +YF+ D Sbjct: 377 IASQVYKRDSVFGQAMEIGMMETAGLGQQN---IDRIIERLKSVTAAQVQAVAQQYFQDD 433 Query: 449 NRTVGLYIPEDHPQRAEIPP 468 TV +P + PP Sbjct: 434 TLTVATLVPLPLAGKKPAPP 453 Score = 58.9 bits (141), Expect = 2e-09, Method: Compositional matrix adjust. Identities = 84/392 (21%), Positives = 165/392 (42%), Gaps = 47/392 (11%) Query: 517 LALLPKQSRGQTVSVSMN-LEFGDADSLFGQRAVAQLTAAMLMRGTSTLDRRQLADEFTR 575 + ++ K+ R +V M + G D + G V+ M+ +GT R EF+R Sbjct: 38 MKVIVKEDRRAPTAVQMVWYKVGGIDEVNGLTGVSHALEHMMFKGT----RNHKVGEFSR 93 Query: 576 L--KING----------NVYRFQTTRENLNAAIALAGEVLRHPRMDPAEFAQLKNETVAA 623 L ++ G Y Q + +L +AL + + + + D AEFA+ E Sbjct: 94 LVAELGGQENAFTANDFTAYFQQIEKSHLEKVMALEADRMANLQFDAAEFAK---EIRVI 150 Query: 624 LEATRKEPD---------ARAAEALQLHFDHYPAGDWRAARTLEERIAAVQAVTLEQVQA 674 +E R D A A A H H+P W + +Q ++++ + A Sbjct: 151 MEERRWRTDDQPMGLLNEALNAAAWTAHPYHHPVVGW---------MDDLQHMSVQDIAA 201 Query: 675 FHRRFYGASHGEVAIVGDFDAQAARSAIDQAFAGW-SSAAPYARILRTWSDIAPKRILID 733 ++R++Y ++ + + GD DAQ + + F + P + + +R+ + Sbjct: 202 WYRQWYAPNNATLVVAGDVDAQRVLALARKYFGKIPARRLPAGKPQNEPQQLGMRRVTVK 261 Query: 734 TPDKENGVYLARQNLRLRDDDPD---YATLVVANYLLGASSLKSRLGDRVRQQDGLSYGI 790 P + V +A + LR + D YA V++ L G + +RL + + G + + Sbjct: 262 APAENPYVVMAWKTPALRQVEQDQDVYALDVLSAVLDGYDN--ARLSASLVRTGGKATAV 319 Query: 791 NSGLQIGAISDAGYLVIHAIAAPQNMDKVDAAVREELDRLLKDGFSEDELLRAKSGILQQ 850 + A + + + A +++ +R E++R+ ++G SE EL R K+ ++ Sbjct: 320 GASYSGVARGPVLFTLEGSPAEGVTTAQLEGLLRGEVERIAREGVSEQELQRVKTQLIAS 379 Query: 851 RNQQRSSDGGMATGWTALLNLDRSFLWQQQLD 882 + +R S G A + ++ + L QQ +D Sbjct: 380 QVYKRDSVFGQA---MEIGMMETAGLGQQNID 408 >gi|300313429|ref|YP_003777521.1| zinc protease-like signal peptide protein [Herbaspirillum seropedicae SmR1] Length = 438 Score = 97.1 bits (240), Expect = 6e-21, Method: Compositional matrix adjust. Identities = 103/415 (24%), Positives = 175/415 (42%), Gaps = 53/415 (12%) Query: 529 VSVSMNLEFGDADSLFGQRAVAQLTAAMLMRGTS----TLDRRQLADEFTRLKIN----- 579 + VS+ + G G+ +A+LT A L RG + TL Q+ D F + Sbjct: 48 LDVSVQFDAGQRRDPAGKAGLAELTVASLTRGVTDASGTLTEAQILDGFADVAAQQHDGA 107 Query: 580 -----GNVYRFQTTRENLNAAIALAGEVLRHPRMDPAEFAQLKNETVAALEATRKEPDAR 634 G R ++ AA+ L +L HP A + + +A ++ +P+ Sbjct: 108 GQDRAGVSLRTLSSPAEREAALTLLARMLAHPSFPQASLERDRALAIANIKEELTKPEVI 167 Query: 635 AAEALQLHFDHYPAGDWRAARTLEERIAAVQAVTLEQVQAFHRRFYGASHGEVAIVGDFD 694 A +A F H G A E A++QA+T E +QAFHR Y A+ +A++GD + Sbjct: 168 AEKA----FMHAAYGSHPYAMDASE--ASMQAITREDLQAFHRAHYVANRAVIALIGDIN 221 Query: 695 AQAAR---SAIDQAFAGWSS-------AAPYARILRTWSDIAPKRILIDTPDKENGVYLA 744 + AR SA+ + ++ AP R + ILI P + G Sbjct: 222 LEQARAIASALTRELPQGAALPALPPVVAPKGSEERIAHPASQSHILIGAPAIQRG---- 277 Query: 745 RQNLRLRDDDPDYATLVVANYLLGASSLKSRLGDRVRQQDGLSYGINSGLQIGAISDAGY 804 DPD+ L V NY+LG SRL D VR++ GLSY + SG ++ G Sbjct: 278 ---------DPDFFALTVGNYVLGGGGFVSRLTDEVREKRGLSYSVYSGFS--PLAQPGP 326 Query: 805 LVIHAIAAPQNMDKVDAAVREELDRLLKDGFSEDELLRAKSGILQQRNQQRSSDGGMATG 864 I + + R LD+ +++G + EL AK + + S+ + Sbjct: 327 FQIGLQTKKEQTAEALRVTRVTLDKFMQEGPTAAELKAAKDNLAGGFALRIDSNAKLLEN 386 Query: 865 WTAL----LNLDRSFLWQQQLDLKLADLTLAQLNAAVRKYIDPARMTVVMARDAA 915 + + L LD W + ++ +++ + AA RK++ P ++ ++ ++A Sbjct: 387 LSVIGFYGLPLDYLDHWIE----RIRAVSVQDVRAAFRKHVHPEELSTIIVGESA 437 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.132 0.380 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,797,776 Number of extensions: 120819 Number of successful extensions: 435 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 425 Number of HSP's successfully gapped: 4 Length of query: 920 Length of database: 1,619,870 Length adjustment: 94 Effective length of query: 826 Effective length of database: 1,174,780 Effective search space: 970368280 Effective search space used: 970368280 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 135 (56.6 bits)