BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3419.p (447 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312188|ref|YP_003776280.1| hemolysin secretion-related tra... 581 e-167 gi|300312349|ref|YP_003776441.1| hemolysin-type secretion transm... 152 6e-38 gi|300311130|ref|YP_003775222.1| Type I antifreeze protein:HlyD ... 106 3e-24 >gi|300312188|ref|YP_003776280.1| hemolysin secretion-related transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 447 Score = 581 bits (1497), Expect = e-167, Method: Compositional matrix adjust. Identities = 292/447 (65%), Positives = 362/447 (80%) Query: 1 MKLLQWMAAWRALWQRYLGIFLHFWRVRKSLRTDHYNQQEAQFLPAVLALQESPASPTLR 60 M L AW LW+RYL +F H WR R R+D +N+ EA+FLPA L+LQE+P S +LR Sbjct: 1 MSLRHRWQAWTDLWRRYLRVFGHAWRQRGHYRSDFFNKDEAEFLPAALSLQEAPDSRSLR 60 Query: 61 WTGRILMSLVAFFFLWAIFGRIDIIVHATGKIIPSSRTKTIASIDVASVKALHVVEGQTV 120 WT R+L+ +V F LW++ GRIDI+V A GK+IPS+RTKTIAS+DV++V+A+ V EGQ V Sbjct: 61 WTARLLVGMVIFALLWSLLGRIDIVVSANGKVIPSARTKTIASVDVSAVRAILVSEGQLV 120 Query: 121 KAGELLIELDSSSSDAEHDKAADAVAQARLQLARVNALREAMASGASPRLPQVEAATSAQ 180 KAGE+LIELDSSSSDAE DKAADAVAQARLQ AR A+ EA+ + +P L QV AQ Sbjct: 121 KAGEVLIELDSSSSDAERDKAADAVAQARLQAARAQAMMEAIQNLRTPELAQVPGVAQAQ 180 Query: 181 WEAAQRQLESQYRDVKSKLDRLDDEIARYGAALPLARQLAEDYQALLADNTVSRHAWQEK 240 WEA + QL +QY+D +++L RLDDEI+RYGAAL LA Q A DYQ L+AD+TVSRHAW EK Sbjct: 181 WEAVRSQLVAQYQDFRARLGRLDDEISRYGAALKLATQRASDYQTLVADHTVSRHAWLEK 240 Query: 241 EQARIELQGQLADARNQRSALIAQTLKEAHDEGIDASKRIEAALQDQRRAGEHSKLLKLV 300 EQAR++LQGQL DARNQR+ L+AQT K+AHD I+A K IEA+ QDQRRA EHSKLLKLV Sbjct: 241 EQARVDLQGQLDDARNQRATLVAQTRKDAHDSRIEADKTIEASQQDQRRADEHSKLLKLV 300 Query: 301 APVSGTVQQLNVHTVGGVVPAAQPLMQIVPQEAAVEVEAFLENKDIGFVQVGQDASVKIE 360 APV+GTVQQL VHT+GGVVPAAQPLMQIVPQ++ +EVEA LENKD+GFV++GQ A VKI+ Sbjct: 301 APVTGTVQQLTVHTIGGVVPAAQPLMQIVPQDSQIEVEAMLENKDVGFVEIGQAAEVKID 360 Query: 361 AFDYTKYGTVPAAVRHVSHDAIEDEKRGLIYASKIVLARHTLAAEGRTLALAPGMSVTVE 420 AFDYTKYGT+PA V H+S DAI+DEK+GL+Y+++IVL + +L +GR + L+ GM+ VE Sbjct: 361 AFDYTKYGTLPARVVHISRDAIQDEKKGLLYSARIVLDQSSLIVDGRKVQLSAGMAANVE 420 Query: 421 IRTGTRRVIEYVLSPLIRHQRESLNER 447 I+TG RRVIEY LSPLIRHQ+E+L+ER Sbjct: 421 IKTGNRRVIEYALSPLIRHQKEALHER 447 >gi|300312349|ref|YP_003776441.1| hemolysin-type secretion transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 386 Score = 152 bits (383), Expect = 6e-38, Method: Compositional matrix adjust. Identities = 120/394 (30%), Positives = 205/394 (52%), Gaps = 27/394 (6%) Query: 60 RWTGRILMSLVAFFFLWAIFGRIDIIVHATGKIIPSSRTKTIASIDVASVKALHVVEGQT 119 +W R+ M+ V LWA +ID + A G++I SRT+++ + D VK + V EG+ Sbjct: 14 QWVLRLSMASVLVLLLWAAVSQIDQVTRAQGQVIAVSRTQSVQAPDGGPVKQILVKEGEA 73 Query: 120 VKAGELLIELDSSSSDAEHDKAADAVAQARLQLARVNALREAMASGASPRLPQVEAATSA 179 VK G+LL+ L+ + + VA R+ LAR+ A P L + Sbjct: 74 VKQGQLLMVLEQDRVQTSLNDSRAKVAALRISLARLRAEVYGHPLNFEPDLLDYK----- 128 Query: 180 QWEAAQRQLESQYRDVKSKLDRLDDEIARYGAALPLARQLAEDYQALLADNTVSRHAWQE 239 ++ A Q+ L VK K+ +D ++A L LA Q E ++L V R Sbjct: 129 EYIANQKDLY-----VKRKI-LIDQDLASLQDMLGLANQELEMNKSLERTGDVGRADVLR 182 Query: 240 KEQARIELQGQLADARNQ--RSALIAQTLKEAHDEGIDASKRIEAALQDQRRAGEHSKLL 297 ++ +++ Q+ + RN+ + AL+ T + E ++ + L+D+ + EH+ Sbjct: 183 LQRNVADIRAQINNKRNKYFQEALVDMTKVQ---EDLNTQRE---QLEDRTQVLEHT--- 233 Query: 298 KLVAPVSGTVQQLNVHTVGGVVPAAQPLMQIVPQEAAVEVEAFLENKDIGFVQVGQDASV 357 +L AP G V+ + V T+GGVV + +M+I+P + + VEA + D +V+VGQ A+V Sbjct: 234 ELKAPADGVVKNIKVTTIGGVVRSGDVVMEILPVDNLI-VEAKVSTADSAYVKVGQAANV 292 Query: 358 KIEAFDYTKYGTVPAAVRHVSHDAIEDEKR-GLIYASKIVLARHTLAAEGR---TLALAP 413 K++A+DY+ YGT+ V +VS D + DE R G + ++ +A +GR + + P Sbjct: 293 KLDAYDYSIYGTLNGHVVYVSPDTLLDETRQGAMPYYRVHIALEEAVFKGRKADAIQVKP 352 Query: 414 GMSVTVEIRTGTRRVIEYVLSPLIRHQRESLNER 447 GM+ TVEI+ TR V+ ++ P+ + +ES+ ER Sbjct: 353 GMTATVEIKANTRTVLSFLTKPIAKTLQESMGER 386 >gi|300311130|ref|YP_003775222.1| Type I antifreeze protein:HlyD family secretion protein [Herbaspirillum seropedicae SmR1] Length = 457 Score = 106 bits (265), Expect = 3e-24, Method: Compositional matrix adjust. Identities = 108/407 (26%), Positives = 196/407 (48%), Gaps = 45/407 (11%) Query: 76 WAIFGRIDIIVHATGKIIPSSRTKTIASIDVASVKALHVVEGQTVKAGELLIELDSSSSD 135 W++F ID++V +G+I+ S + I ++ V A+ V EG VK G+ LI + ++ Sbjct: 58 WSVFAPIDMVVRGSGRIVASEHNQVIQHLEGGIVSAILVREGAAVKKGQTLISISDVRAN 117 Query: 136 AEHDKAADAVAQARLQLARVNALREAMASGASP-RLPQVEAATSAQWEAAQRQLESQYRD 194 A+ + + R ++AR++A ASGAS ++P ++ Q ++ Sbjct: 118 ADLGEGRVKILGLRAKIARLSA----EASGASSLQMPDDLERGDPAVQSEQAAFAARSSK 173 Query: 195 VKSKLDRLDDEIARYGAAL--PLARQ--------LAEDYQALLADNTVSRHAWQEKEQAR 244 + ++ L ++ A+ A L ++RQ LA+ L+ D V A Q ++ Sbjct: 174 LMQEMSVLREQSAQRQAELGEAISRQKSQASELELAQRQYKLIHDMLVRNAASQLED--- 230 Query: 245 IELQGQLADARNQRSALIAQ------TLKEAHDEGIDASKRIEAALQDQRRAGEHSKLLK 298 I+ ++ DA+++ +A A + EA + +A+ R + + + A E ++L + Sbjct: 231 IQAASRVQDAQSKLAATNAMIPRLNAAIAEAQAKQGEAASRFRSDARTELTAAE-TELAR 289 Query: 299 L----------------VAPVSGTVQQLNVHTVGGVVPAAQPLMQIVPQEAAVEVEAFLE 342 L AP+ G V ++ ++T+GGVV P++++ P + + +EA + Sbjct: 290 LQEEIKSRNDRVSRSEVKAPMDGVVNRVMINTIGGVVRPGDPIIEMTPSDDKLVIEARIS 349 Query: 343 NKDIGFVQVGQDASVKIEAFDYTKYGTVPAAVRHVSHDAIEDE--KRG-LIYASKIVLAR 399 D + G A VK+ A+DY YG + V +S D + DE RG Y KI + R Sbjct: 350 PTDRAELVTGARARVKVSAYDYGVYGAMDGVVTEISADTVPDEGSPRGERFYRLKIEVKR 409 Query: 400 HTLAAEGRTLALAPGMSVTVEIRTGTRRVIEYVLSPLIRHQRESLNE 446 + + LAL PGM+ + +I G R V +Y++SPL R + +L E Sbjct: 410 GHDELQ-QKLALMPGMTASADIIVGRRTVWQYLMSPLSRFGQGALRE 455 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.130 0.367 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,338,450 Number of extensions: 53220 Number of successful extensions: 247 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 246 Number of HSP's successfully gapped: 3 Length of query: 447 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 359 Effective length of database: 1,203,190 Effective search space: 431945210 Effective search space used: 431945210 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)