BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3406.p (164 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312746|ref|YP_003776838.1| dihydrofolate reductase oxidore... 282 8e-78 >gi|300312746|ref|YP_003776838.1| dihydrofolate reductase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 164 Score = 282 bits (722), Expect = 8e-78, Method: Compositional matrix adjust. Identities = 139/164 (84%), Positives = 152/164 (92%) Query: 1 MSTSSGQLTIIVATDLNNGIGIRNTLPWHLPEDLAHFKRTTSGHTILMGRKTFESIGRPL 60 M+TSSGQLT+IVATDLNNGIGI NTLPW LPEDLAHFKRTTSGH ILMGRKTF+SIGRPL Sbjct: 1 MTTSSGQLTLIVATDLNNGIGINNTLPWRLPEDLAHFKRTTSGHAILMGRKTFDSIGRPL 60 Query: 61 PNRRNIVITRNALWQHSGVETTGSLAQAAALAGAAPAFIIGGAQVYAEALPLVDRLIITR 120 PNRRNIVITRNA WQH GVET GSL +AAAL APAFIIGGAQ+YAEALPL DRLIITR Sbjct: 61 PNRRNIVITRNAGWQHDGVETAGSLQEAAALCAGAPAFIIGGAQIYAEALPLADRLIITR 120 Query: 121 IARAFECDAFFPAIDEELWQETAREQHHAETSGLDYAFVTYERR 164 IA+ F+CDAFFPA+D+++WQETARE+HH+ETSGLDYAFVTYERR Sbjct: 121 IAQTFDCDAFFPALDQQVWQETAREEHHSETSGLDYAFVTYERR 164 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.134 0.403 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 437,418 Number of extensions: 14767 Number of successful extensions: 47 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 47 Number of HSP's successfully gapped: 1 Length of query: 164 Length of database: 1,619,870 Length adjustment: 78 Effective length of query: 86 Effective length of database: 1,250,540 Effective search space: 107546440 Effective search space used: 107546440 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 127 (53.5 bits)