BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3402.p (496 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312742|ref|YP_003776834.1| outer membrane efflux protein [... 819 0.0 gi|300313898|ref|YP_003777990.1| outer membrane drug efflux lipo... 215 5e-57 gi|300311360|ref|YP_003775452.1| outer membrane drug efflux lipo... 208 9e-55 gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbas... 205 6e-54 gi|300309656|ref|YP_003773748.1| outer membrane drug efflux prot... 197 2e-51 >gi|300312742|ref|YP_003776834.1| outer membrane efflux protein [Herbaspirillum seropedicae SmR1] Length = 492 Score = 819 bits (2115), Expect = 0.0, Method: Compositional matrix adjust. Identities = 434/473 (91%), Positives = 454/473 (95%) Query: 24 TRLTLAVTLVLTVAGCVTREVQTDPILEMQVPDKWGGGASADVAHAEQLWPDTDWWKQFG 83 TR+ LA LVL V GC REVQTDPILEMQVPDKWGGGASADVAHAEQLWPDTDWWKQFG Sbjct: 20 TRVALAAALVLAVGGCAMREVQTDPILEMQVPDKWGGGASADVAHAEQLWPDTDWWKQFG 79 Query: 84 SSELSELVQEGQRNNYEIAAAYSRVKQAQAQARIAGAPLLPNVGLSAGATREMPISGGTP 143 S+ELSELV++GQRNNYEIAAAYSRVKQA+AQARIAGAPLLP+ G++AGA RE+PISGGTP Sbjct: 80 SNELSELVEQGQRNNYEIAAAYSRVKQAEAQARIAGAPLLPSAGINAGAAREIPISGGTP 139 Query: 144 TNTANASLQVSYEIDFWGKNRAGVEAAEATLRANRYDQQTVALTVTSGIVSTYLQVLSLR 203 TNTANASLQVSYEIDFWGKNRAGVEAAEATLRANRYD++TVALTVTSGIVSTYLQVLSLR Sbjct: 140 TNTANASLQVSYEIDFWGKNRAGVEAAEATLRANRYDRETVALTVTSGIVSTYLQVLSLR 199 Query: 204 DRLEIARQNVNNAERVLRLVEAQSRAGAASPLDLARQRSALANQRQLIPDLQQQEQDAQT 263 DRL IARQNV NAERVLRLVEAQSRAG+ASPLDLARQRSALANQRQLIPDLQQQEQDAQT Sbjct: 200 DRLAIARQNVQNAERVLRLVEAQSRAGSASPLDLARQRSALANQRQLIPDLQQQEQDAQT 259 Query: 264 ALAILLGRPPQNFKVEEKGLAKIQMPRISAGLPSELLTRRPDIRRAEANLAAANANVAVA 323 ALAILLGRPPQNFKVEEKGLA+IQMPRISAGLPSELLTRRPDIRRAEA+LAAANANVAVA Sbjct: 260 ALAILLGRPPQNFKVEEKGLARIQMPRISAGLPSELLTRRPDIRRAEASLAAANANVAVA 319 Query: 324 RAALFPSISLTGSTGAQSSALLSLFDGPNLFASLGASLIAPIFDGGNLRNQQALAEAQKE 383 RAALFPSI+LTGSTGAQSSALLSLFDGPNLFA+LGA LIAPIFDGG LR Q+ LA AQKE Sbjct: 320 RAALFPSITLTGSTGAQSSALLSLFDGPNLFANLGAGLIAPIFDGGKLRGQRDLAIAQKE 379 Query: 384 ELVQVYRQRVITSLSEVEKALGAIRSLEERYRLKVGEVDQARFAFELAEIRYRAGAEDLL 443 ELVQVYRQRVITSLSEVEKALGAIRSLEERYRLKVGEVDQARFAFELAEIRYRAGAEDLL Sbjct: 380 ELVQVYRQRVITSLSEVEKALGAIRSLEERYRLKVGEVDQARFAFELAEIRYRAGAEDLL 439 Query: 444 VVLDTQRTLSEAQNQLGQIKLQRLQATVSLYKALGGGWQDKQVPTETGTASVS 496 VVLDTQRTLSEAQNQLGQIKLQRLQATVSLYKALGGGWQD Q P ++GT+SVS Sbjct: 440 VVLDTQRTLSEAQNQLGQIKLQRLQATVSLYKALGGGWQDSQAPGDSGTSSVS 492 >gi|300313898|ref|YP_003777990.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 486 Score = 215 bits (548), Expect = 5e-57, Method: Compositional matrix adjust. Identities = 162/471 (34%), Positives = 233/471 (49%), Gaps = 16/471 (3%) Query: 38 GCVTREVQTDPILEMQVPDKWGGGASADVAHAEQLWPDTDWWKQFGSSELSELVQEGQRN 97 GC + T P ++M P W A P WW++FG ++L+ L Q+ Sbjct: 18 GCASGPDYTRPQVDM--PAAWTSEAPWRSMQPADATPRGPWWERFGDAQLNALQQQALAG 75 Query: 98 NYEIAAAYSRVKQAQAQARIAGAPLLPNVGLSAGATREMPISGGTPTNTANA-------- 149 N +A A +R+ QA+AQ + GA P V L+A A R IS P N+ Sbjct: 76 NQTLAIATARLAQARAQLNVTGAAQTPQVNLNARAARSR-ISANRPLTNYNSPNFSTVQN 134 Query: 150 ----SLQVSYEIDFWGKNRAGVEAAEATLRANRYDQQTVALTVTSGIVSTYLQVLSLRDR 205 VSYE+DF+G+ + VEAA A + D + L +T+ + + Y + L Sbjct: 135 DFALGASVSYEVDFFGRVQRSVEAAGAAAEQSAADLENTRLLLTAELATNYFNLRELDVE 194 Query: 206 LEIARQNVNNAERVLRLVEAQSRAGAASPLDLARQRSALANQRQLIPDLQQQEQDAQTAL 265 L++ R+ + + L L A+ GA S LD+A+Q++ L + I L +Q + A+ Sbjct: 195 LDVVRRAIALQRQALELATARHDLGATSGLDMAQQQALLDSTLTQIDLLGRQRAQYEHAI 254 Query: 266 AILLGRPPQNFKVEEKGLAKIQMPRISAGLPSELLTRRPDIRRAEANLAAANANVAVARA 325 A L G P F + L I P I G+PS++L RRPDI AE +AAANA + VA A Sbjct: 255 ATLTGTPAPAFSITPS-LTPITPPAIPVGVPSDVLERRPDIAAAERAMAAANAQIGVASA 313 Query: 326 ALFPSISLTGSTGAQSSALLSLFDGPNLFASLGASLIAPIFDGGNLRNQQALAEAQKEEL 385 A +PS L S G S +LF+ P+L SLG S +FDGG LR ++A E Sbjct: 314 AYYPSFMLQPSYGVDSRNWATLFNAPSLLWSLGVSASQSLFDGGRLRAGVDFSQAGYEAT 373 Query: 386 VQVYRQRVITSLSEVEKALGAIRSLEERYRLKVGEVDQARFAFELAEIRYRAGAEDLLVV 445 V YR+ V+T++ EVE + + +LE Y + AR E+A RY GA L V Sbjct: 374 VASYRRTVLTAMQEVEDGITGLAALERAYAQSQLAIASARRVLEIASSRYEGGATPYLDV 433 Query: 446 LDTQRTLSEAQNQLGQIKLQRLQATVSLYKALGGGWQDKQVPTETGTASVS 496 + Q++L ++ Q Q+ QRL +V L KALGG WQ + P E A S Sbjct: 434 ITAQQSLLNSERQAAQLMGQRLLVSVFLIKALGGDWQSRTPPAEAAGAGPS 484 >gi|300311360|ref|YP_003775452.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 470 Score = 208 bits (529), Expect = 9e-55, Method: Compositional matrix adjust. Identities = 152/452 (33%), Positives = 236/452 (52%), Gaps = 20/452 (4%) Query: 40 VTREVQTDPILEMQVPDKW---GGGASADVAHAEQLWPDTDWWKQFGSSELSELVQEGQR 96 +TR T P+ Q P W G ADV WW+ FG + L+ L+ E R Sbjct: 29 LTRSAYT-PV-STQTPASWQQRGSQPEADVQR---------WWQGFGDATLTALIDEALR 77 Query: 97 NNYEIAAAYSRVKQAQAQARIAGAPLLPNVGLSAGATREMPISGG-TPTNTANASLQVSY 155 N ++A A ++++AQAQA +A +P + S GA+ + G T + +S+++SY Sbjct: 78 KNNDLALAAIKLRRAQAQAGLADDAFVPALSASLGASSSKQLDGDRISTRASQSSVKLSY 137 Query: 156 EIDFWGKNRAGVEAAEATLRANRYDQQTVALTVTSGIVSTYLQVLSLRDRLEIARQNVNN 215 E+D WG+ A + + A D+Q+ ALT+ + Y L R+ A Q++ Sbjct: 138 EVDLWGRLAAASDLSRWEALATEQDRQSTALTLIGTTANLYWTGAYLNQRVATAEQSIAY 197 Query: 216 AERVLRLVEAQSRAGAASPLDLARQRSALANQRQLIPDLQQQEQDAQTALAILLGRPPQN 275 AE+ L LV Q +AGA S +++ + L +Q+ +L QQ + ALAIL +PP Sbjct: 198 AEKTLELVRTQYQAGAVSSVEVLSAQQNLYSQQSSYTELVQQRVENDNALAILFDQPPGK 257 Query: 276 FKVEEKGLAKIQMPRISAGLPSELLTRRPDIRRAEANLAAANANVAVARAALFPSISLTG 335 L + +P ++AG+P+ELL RRPD+R E + +A A V AR +P +SLTG Sbjct: 258 AIALPARLPEQALPVLAAGVPAELLGRRPDLRALELRIRSALAGVDQARLNYYPQLSLTG 317 Query: 336 STGAQSSALLSLFDGPNLFASLGASLIAPIFDGGNLRNQQALAEAQKEELVQVYRQRVIT 395 + G S ALL L P A+LG +L P ++ + +++A + V +R+ + T Sbjct: 318 TLGTSSDALLRLVQNP--VATLGGALTLPFLQHSEMKFKVGISQADFDTAVLNFRKGLYT 375 Query: 396 SLSEVEKALGAIRSLEERYRLKVGEVDQARFAFELAEIRYRAGAEDLLVVLDTQRTLSEA 455 + SEVE AL A + + L V +D A+ A L E+RYRAG+ L + LD Q A Sbjct: 376 AFSEVENALSARQQYARQQALLVRNLDAAQAAERLLEVRYRAGSATLQLWLDQQDKRRTA 435 Query: 456 QNQLGQIKLQRLQATVSLYKALGGGWQDKQVP 487 + L Q++L +L+ ++LY+ALGG D VP Sbjct: 436 EISLAQVRLNQLKNQMTLYQALGG---DASVP 464 >gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 483 Score = 205 bits (522), Expect = 6e-54, Method: Compositional matrix adjust. Identities = 166/493 (33%), Positives = 252/493 (51%), Gaps = 33/493 (6%) Query: 10 ASSSLPRLMRLTRPTRLTLAVTLVLTVAGCV---TREVQTDPILEMQVPDKWGGGASADV 66 A+ S+PR RL + A+ + + GC T E P+ E P AD Sbjct: 2 ANLSMPRSPRLLH---MCAALVMAGVLGGCSLAPTYERPQAPV-ESSYP--------ADA 49 Query: 67 AHAEQLWPDTDWWKQFGSSE-LSELVQEGQRNNYEIAAAYSRVKQAQAQARIAGAPLLPN 125 A +L W+QF + L +L+ NN ++ A +++A+AQ +IA A LLPN Sbjct: 50 AGRAELQAVNLGWRQFFPDQRLQQLIAAALENNRDLRTAVLNIEEARAQYQIARADLLPN 109 Query: 126 VGLSAGATREMPISG----GTPT--NTANASLQV-SYEIDFWGKNRAGVEAAEATLRANR 178 + LS A R + G PT N+ L V +YE+DF+G+ R +AA A+ + Sbjct: 110 LNLSGNAARTRTAASQSLTGQPTISNSYQVGLAVPAYELDFFGRVRNLKDAALASYLSTE 169 Query: 179 YDQQTVALTVTSGIVSTYLQVLSLRDRLEIARQNVNNAERVLRLVEAQSRAGAASPLDLA 238 Q++ +++ + + YL S ++L++A + + + + L L + + GA++ LD Sbjct: 170 EAQKSAQISLVAQVAQAYLAERSYAEQLKLADETLKSRQSNLDLAQKRLDVGASTALDFR 229 Query: 239 RQRSALANQRQLIPDLQQQEQDAQTALAILLGR-----PPQNFKVEEKGLAKIQMPRISA 293 ++ + + R L++Q A+ AL +L+G+ PP +EK + I A Sbjct: 230 LTQTLVESARVAKTQLERQRAQAENALTLLVGKKIGELPPAQLLSDEKIVTDI-----PA 284 Query: 294 GLPSELLTRRPDIRRAEANLAAANANVAVARAALFPSISLTGSTGAQSSALLSLFDGPNL 353 GLPS+LLT RPDIR AE +L AANAN+ ARAA FP ISLTG+ G+ SS L LF+ + Sbjct: 285 GLPSDLLTNRPDIRSAEQSLLAANANIGAARAAFFPRISLTGNFGSASSTLGGLFESGSG 344 Query: 354 FASLGASLIAPIFDGGNLRNQQALAEAQKEELVQVYRQRVITSLSEVEKALGAIRSLEER 413 S G SL PIFD G LAE +K + V Y + + T+ EV AL A SL+E+ Sbjct: 345 AWSFGPSLSLPIFDFGRNSANLDLAEVRKNKAVVTYEKAIQTAFREVADALVARGSLDEQ 404 Query: 414 YRLKVGEVDQARFAFELAEIRYRAGAEDLLVVLDTQRTLSEAQNQLGQIKLQRLQATVSL 473 + + + + +L ++R++ G L LD QR L AQ L Q + RL + L Sbjct: 405 VKAQEAFLAAQQERLKLTDLRFKNGIASSLDQLDAQRDLFSAQQALIQARQLRLNNAIDL 464 Query: 474 YKALGGGWQDKQV 486 YKALGGG + V Sbjct: 465 YKALGGGLNENTV 477 >gi|300309656|ref|YP_003773748.1| outer membrane drug efflux protein [Herbaspirillum seropedicae SmR1] Length = 504 Score = 197 bits (500), Expect = 2e-51, Method: Compositional matrix adjust. Identities = 152/426 (35%), Positives = 227/426 (53%), Gaps = 17/426 (3%) Query: 77 DWWKQFGSSELSELVQEGQRNNYEIAAAYSRVKQAQAQARIAGAPLLPNVGLSAGATREM 136 +WWK FG +L+ L Q+ N ++ AA +R+ QA+A R A A P V GATR+ Sbjct: 77 EWWKIFGDEKLNALEDTAQQANQDLKAAAARLGQARALTRDARAGYFPQVDAGVGATRQR 136 Query: 137 P--ISGGTPTNTAN-------ASLQVSYEIDFWGKNRAGVEAAEATLRANRYDQQTVALT 187 P S G P + A + VSYE+D +G+ + V+AA A + + +V L Sbjct: 137 PSPASQGLPADADTRPNTLYRAQVNVSYEVDLFGRVASTVDAATADAQRSEALFHSVLLA 196 Query: 188 VTSGIVSTYLQVLSLRDRLEIARQNVNNAERVLRLVEAQSRAGAASPLDLARQRSALANQ 247 + + + Y V L + R V+ + LRLV+ + G S +D+AR R+ LA+ Sbjct: 197 LQADVAQQYFLVRELDAAAALYRGTVDLRQESLRLVQRRFDEGDISEVDVARARAELASA 256 Query: 248 RQLIPDLQQQEQDAQTALAILLGRPPQNFKVEEKGLAKIQMPRISAGLPSELLTRRPDIR 307 + + +Q A+ ALAILLGR P F + + L ++ + + GLPS LL RRPDI Sbjct: 257 QSEALGIARQRATAEHALAILLGRTPAEFSLPPQPLTRLAVT-VPPGLPSALLERRPDIA 315 Query: 308 RAEANLAAANANVAVARAALFPSISLTGSTGAQSSALLSLFDGPN----LFASLGASLIA 363 AE +AAANA V VA++A FPS++LTG G +S+ L LF+ + L +G +L Sbjct: 316 AAERAMAAANARVGVAKSAFFPSLNLTGGLGYESAQLGDLFNWSSRTFLLGPLVGTALSM 375 Query: 364 PIFDGGNLRNQQALAEAQKEELVQVYRQRVITSLSEVEKALGAIRSLEERYRLKVGEVDQ 423 P+FDGG + A A EE V YR V+ + EVE L +R L+++ R + V Sbjct: 376 PLFDGGRRQAGVDRARASYEEQVASYRGTVLNAFREVEDNLANLRILDQQNRAQDEAVAA 435 Query: 424 ARFAFELAEIRYRAGAEDLLVVLDTQRTLSEAQNQLGQIKLQRLQATVSLYKALGGGWQD 483 + A +L+ +YR GA V+D RT+ + Q + +R ++TV+L +ALGGGW Sbjct: 436 SARAAKLSHTQYREGAVSYFSVIDADRTVLQQQRASVTLDGERARSTVNLIRALGGGW-- 493 Query: 484 KQVPTE 489 VP E Sbjct: 494 -GVPAE 498 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.315 0.129 0.354 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,260,203 Number of extensions: 49310 Number of successful extensions: 302 Number of sequences better than 1.0e-08: 19 Number of HSP's gapped: 253 Number of HSP's successfully gapped: 19 Length of query: 496 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 407 Effective length of database: 1,198,455 Effective search space: 487771185 Effective search space used: 487771185 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 132 (55.5 bits)