BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3401.p (353 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312741|ref|YP_003776833.1| 2-nitropropane dioxygenase [Her... 504 e-144 gi|300312796|ref|YP_003776888.1| 2-nitropropane dioxygenase [Her... 202 3e-53 gi|300313977|ref|YP_003778069.1| 2-nitropropane dioxygenase [Her... 56 4e-09 >gi|300312741|ref|YP_003776833.1| 2-nitropropane dioxygenase [Herbaspirillum seropedicae SmR1] Length = 356 Score = 504 bits (1297), Expect = e-144, Method: Compositional matrix adjust. Identities = 281/349 (80%), Positives = 312/349 (89%) Query: 3 TSLLQRLGLAHPIIQAPMAGSSTVAMAAAASNAGALGSLALGAASVAQARQALAQLNALT 62 T L+QRLGLAHPIIQAPMAGSSTV MAAAASNAGALGSLALGA++ QAR+ALAQL+ALT Sbjct: 5 TPLMQRLGLAHPIIQAPMAGSSTVVMAAAASNAGALGSLALGASNAEQAREALAQLHALT 64 Query: 63 EKPFNVNFFCHQPAVLDAARDAAWIQHLQAHLDEFGGSISLPLKEIYPSFIVDTDMQQLM 122 EKP+N+N FCHQPAVLD+ RD AW+ HLQ LDEFGG I+ PLKEIY SF DT MQQ++ Sbjct: 65 EKPYNINVFCHQPAVLDSERDRAWLAHLQPLLDEFGGRIAAPLKEIYQSFNADTQMQQVV 124 Query: 123 LEQPPAVVSFHFGLPEAALIQALKKAGVFLLATATNLEEARQIEAAGIDAIVAQGFEAGG 182 LE PAVVSFHFGLP A +I AL++AG+ L+ATATNL+EARQIEAAG+DAIVAQGFEAGG Sbjct: 125 LEHAPAVVSFHFGLPPADVIGALRQAGILLMATATNLDEARQIEAAGLDAIVAQGFEAGG 184 Query: 183 HRGIFDEHRDEAIGTFALVRLLAQHSRLPIIAAGGIMDGQGIAAALQLGAQAVQLGTAFL 242 HRGIFD +DE IG+FAL+RLL QH+RLP+IAAGGIMDG GIAAALQLGAQAVQ+GTAFL Sbjct: 185 HRGIFDPTKDEQIGSFALLRLLVQHTRLPVIAAGGIMDGHGIAAALQLGAQAVQMGTAFL 244 Query: 243 LTRESAANAAYRHLLKSSRTQHTRVTAAISGRRARGIVNRFMEEIDISAAPAIPAYPAAY 302 LTRES ANA+YR LLKS R QHTRVTAAISGR ARGIVNRFMEEID AAP IPAYPAAY Sbjct: 245 LTRESNANASYRALLKSPRAQHTRVTAAISGRPARGIVNRFMEEIDTPAAPPIPAYPAAY 304 Query: 303 DAGKQLNALASAHEVHEFGAHWAGQGAALIRDGYTVGELIHLLVKEWQA 351 DAGKQL+ALA+A +VHEFGAHWAGQGAALIRDGYTV EL+HLLV+EWQA Sbjct: 305 DAGKQLHALAAARDVHEFGAHWAGQGAALIRDGYTVAELVHLLVQEWQA 353 >gi|300312796|ref|YP_003776888.1| 2-nitropropane dioxygenase [Herbaspirillum seropedicae SmR1] Length = 358 Score = 202 bits (515), Expect = 3e-53, Method: Compositional matrix adjust. Identities = 158/362 (43%), Positives = 200/362 (55%), Gaps = 20/362 (5%) Query: 1 MSTSLLQRLGLAHPIIQAPMAGSSTVAMAAAASNAGALGSLALGAASVAQARQALAQLNA 60 +ST L ++ G+ PI+Q PM G ST M AA SNAG +G+LA V R + + + Sbjct: 5 LSTPLTRKFGIDFPIVQGPMNGGSTPEMVAAVSNAGGIGALAAANFDVQTMRTKVNAIRS 64 Query: 61 LTEKPFNVNFFC-HQPAVLDAA-RDAAWIQHLQAHLDEFGGSISLPLKEIYPSFIVDTDM 118 LT KPF VN F PA RDA + LQ E G LP P + Sbjct: 65 LTTKPFCVNLFVLETPAPTPGEIRDA--LDRLQPIRAELG----LP-PAAAPERYCEEFR 117 Query: 119 QQL----MLEQPPAVVSFHFGLPEAALIQALKKAGVFLLATATNLEEARQIEAAGIDAIV 174 +QL L+ P V SF FGL +AA I+ L AG ++ TATN+ EA +A G DAI Sbjct: 118 EQLDALIALKAP--VASFTFGLLDAASIERLHAAGSLVIGTATNVAEALAWQANGADAIC 175 Query: 175 AQGFEAGGHRGIFDEHRDEA-IGTFALVRLLAQHSRLPIIAAGGIMDGQGIAAALQLGAQ 233 AQG EAGGHRG F H D + IGT ALV + LP+IAAGGIMDG+GIAAAL LGAQ Sbjct: 176 AQGAEAGGHRGTFIGHPDASMIGTLALVPQIVDAVALPVIAAGGIMDGRGIAAALTLGAQ 235 Query: 234 AVQLGTAFLLTRESAANAAYRHLLKSSRTQHTRVTAAISGRRARGIVNRFMEEIDISAAP 293 A QLGTAFL+T ES +AA++ ++ S TR T +GR ARGI+N FM + Sbjct: 236 AAQLGTAFLMTAESPIHAAWQEKIRLSTDTSTRTTRTFTGRPARGILNDFMLRMG-RYEQ 294 Query: 294 AIPAYPAAYDAGKQLNALASAHEVHEFGAHWAGQGAALIR---DGYTVGELIHLLVKEWQ 350 +PAYP + ++ A EF + WAGQ A + R + EL+ +LV E Q Sbjct: 295 ELPAYPVQHALTTEIRKAAGQANRPEFMSLWAGQAAGMSRRRAEAIPAAELVRVLVAETQ 354 Query: 351 AA 352 AA Sbjct: 355 AA 356 >gi|300313977|ref|YP_003778069.1| 2-nitropropane dioxygenase [Herbaspirillum seropedicae SmR1] Length = 324 Score = 56.2 bits (134), Expect = 4e-09, Method: Compositional matrix adjust. Identities = 90/365 (24%), Positives = 139/365 (38%), Gaps = 73/365 (20%) Query: 6 LQRLGLAHPIIQAPMAGSSTVAMAAAASNAGALGSL-ALGA-------ASVAQARQALAQ 57 LQ L L PII +PM +S A+ AA AG +GS AL A + Q ++ LAQ Sbjct: 11 LQDLRL--PIICSPMFIASGPALVAAQCKAGLVGSFPALNARPAELLDTWLTQLQEELAQ 68 Query: 58 LNALTEK----PFNVNFFCHQPAVLDAARDAAWIQHLQAHLDEFGGSISLPLKEIYPSFI 113 A + P VN HQ A A ++H Sbjct: 69 WQAEHAQRKVGPIAVNQIVHQSNDRLAQDVATCVKH------------------------ 104 Query: 114 VDTDMQQLMLEQPPAVVSFHFGLPEAALIQALKKAGVFLLATATNLEEARQIEAAGIDAI 173 Q P ++S P ++ A+ G +L +L A + AG+D + Sbjct: 105 -----------QVPIIIS-SLRAPPQEMLDAVHSYGGIVLHDVISLRHAEKALEAGVDGL 152 Query: 174 VAQGFEAGGHRGIFDEHRDEAIGTFALVRLLAQHSRLPIIAAGGIMDGQGIAAALQLGAQ 233 + A + FALV + + + P++ +G I G + AA +GA Sbjct: 153 ILVAAGA--------GGHAGTLSPFALVGEVRKMFKGPLVLSGAIATGDAVLAAQAMGAD 204 Query: 234 AVQLGTAFLLTRESAANAAYRHLLKSSRTQHTRVTAAISGRRA----RGIVNRFMEEIDI 289 +G+ +L T+ES + AYR + S T +G + IVN ++ ++ Sbjct: 205 FAYMGSRWLATQESNVDQAYRQAIVDSTAADVVYTNLFTGVHGNYLKKSIVNAGLDPDNL 264 Query: 290 SAAPAIPAYPAAYDAGKQLNALASAHEVHEFGAHW-AGQGAALIRDGYTVGELIHLLVKE 348 A D K + + W AGQG L+ D TV E++ L E Sbjct: 265 PEA----------DKTKMNFGSGGGSKAKAWRDIWGAGQGVGLMDDVPTVAEMVDRLEAE 314 Query: 349 WQAAR 353 ++AAR Sbjct: 315 YRAAR 319 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.132 0.378 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 873,012 Number of extensions: 30207 Number of successful extensions: 136 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 131 Number of HSP's successfully gapped: 3 Length of query: 353 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 267 Effective length of database: 1,212,660 Effective search space: 323780220 Effective search space used: 323780220 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 131 (55.1 bits)