BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3397.p (261 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312738|ref|YP_003776830.1| short chain dehydrogenase [Herb... 484 e-138 gi|300310578|ref|YP_003774670.1| beta-hydroxybutyrate dehydrogen... 123 1e-29 gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein... 111 5e-26 gi|300311568|ref|YP_003775660.1| toluenesulfonate Zinc-independe... 111 5e-26 gi|300310595|ref|YP_003774687.1| 3-oxoacyl-(acyl-carrier protein... 109 2e-25 >gi|300312738|ref|YP_003776830.1| short chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 261 Score = 484 bits (1247), Expect = e-138, Method: Compositional matrix adjust. Identities = 237/261 (90%), Positives = 247/261 (94%) Query: 1 MDLNLKDQKVIVTAGAQGIGLAITAAFVEAGAHVHICDISEDFLASARERFGTAPVSYSR 60 MDLNLKDQKVIVTAGAQGIGLAITAAFVEAGAHVHICD+SEDFLASAR RFG APVSYSR Sbjct: 1 MDLNLKDQKVIVTAGAQGIGLAITAAFVEAGAHVHICDVSEDFLASARARFGHAPVSYSR 60 Query: 61 TDVSRASEVDAMFAELAQRWDGRLDVLVNNAGIAGPTLPVEEVDLSGWDQTIAVNLTGPF 120 TDVS EVDAMFA+LAQRW GRLDVL+NNAGIAGPT PVEEV LS WDQT+AVNLTGPF Sbjct: 61 TDVSSEREVDAMFADLAQRWSGRLDVLINNAGIAGPTSPVEEVALSDWDQTLAVNLTGPF 120 Query: 121 LCTRRAVPMLKNNGGGAIVNISSVAGRLGFALRTPYSASKYGVIGLTETWAIELGPCNIR 180 LCTRRAVP+LK NGGGAIVNISSVAGRLGFALRTPYSASKYGVIGLTETWAIELGP +IR Sbjct: 121 LCTRRAVPLLKKNGGGAIVNISSVAGRLGFALRTPYSASKYGVIGLTETWAIELGPSHIR 180 Query: 181 VNAVLPGIVAGARQERIVAAKATAYGIAHEEMRQRLLSKVSLRKMVTAEDVANQVIFICS 240 VNAVLPGIV GARQERIVAAKA AYGI HEEMRQRLLS+VSLRKMVTAED+ANQVIF+CS Sbjct: 181 VNAVLPGIVEGARQERIVAAKAAAYGIGHEEMRQRLLSRVSLRKMVTAEDIANQVIFLCS 240 Query: 241 PAGASISGRSLSVCGNVEVLG 261 PAGASISG+SLSVCGNVEVLG Sbjct: 241 PAGASISGQSLSVCGNVEVLG 261 >gi|300310578|ref|YP_003774670.1| beta-hydroxybutyrate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 260 Score = 123 bits (309), Expect = 1e-29, Method: Compositional matrix adjust. Identities = 85/257 (33%), Positives = 143/257 (55%), Gaps = 12/257 (4%) Query: 5 LKDQKVIVTAGAQGIGLAITAAFVEAGAHVHICDIS-EDFLASARE---RFGTA-PVSYS 59 LKD+ ++T A GIG I + GA V I D++ E A+A E GTA V+ + Sbjct: 3 LKDKVALITGSASGIGKEIAVEYARQGAKVVIADLALEAAKATAEEIVKEGGTAMAVAMN 62 Query: 60 RTDVSRASEVDAMFAELAQRWDGRLDVLVNNAGIAGPTLPVEEVDLSGWDQTIAVNLTGP 119 TD ++VD A+ + G LD++++NAGI + PV E+ L W + +A++L G Sbjct: 63 VTD---ETQVDKGVADAVNTYGG-LDIMISNAGIQIIS-PVAELSLENWRKMLAIHLDGA 117 Query: 120 FLCTRRAVP-MLKNNGGGAIVNISSVAGRLGFALRTPYSASKYGVIGLTETWAIELGPCN 178 FL TR A+ M+K GG+I+ + SV L L+ PY +K+G++GL +T A E Sbjct: 118 FLTTRAAMKAMIKQGRGGSIIYMGSVHSHLASPLKAPYVTAKHGLLGLAKTVAKEGAKDK 177 Query: 179 IRVNAVLPGIVAGARQERIVAAKATAYGIAHEEMRQRLLSKVSLR-KMVTAEDVANQVIF 237 IR N + PG V E+ + +A +GI+ E++ ++++ K ++ + T +DVA +F Sbjct: 178 IRTNVICPGFVRTPLVEKQIPEQAKEFGISEEDVIKKIMLKDTVDGEFTTTQDVAQTAVF 237 Query: 238 ICSPAGASISGRSLSVC 254 + + +++G+S+ V Sbjct: 238 LAAFPTNALTGQSVVVS 254 >gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein) reductase [Herbaspirillum seropedicae SmR1] Length = 256 Score = 111 bits (278), Expect = 5e-26, Method: Compositional matrix adjust. Identities = 86/252 (34%), Positives = 124/252 (49%), Gaps = 24/252 (9%) Query: 11 IVTAGAQGIGLAITAAFVEAGAHVHICDI-------SEDFLASARERFGTAPVSYSRTDV 63 +VT GA GIG +I+ G V + DI + D L +A R AP+ + D+ Sbjct: 12 LVTGGAMGIGASISQQLAAKGHTVLVADINLDAASKTADALVAAGHR--AAPL---QMDL 66 Query: 64 SRASEVDAMFAELAQRWDGRLDVLVNNAGIAGPTLPVEEVDLSGWDQTIAVNLTGPFLCT 123 + A FA + + + GR DVLVNNAG+A T + L W QT+ VN+TG L Sbjct: 67 GSPESIAAAFAHIEKDY-GRCDVLVNNAGVA-RTYSFLDYPLENWLQTMNVNVTGVLLAG 124 Query: 124 RRAVPMLKNNGGGAIVNISSVAGRLGFALRTPYSASKYGVIGLTETWAIELGPCNIRVNA 183 + A ++ G IVNISS++G A RT Y SK VIGLT AIEL I VN+ Sbjct: 125 QHAARLMVKQQWGRIVNISSISGIRAGAGRTAYGTSKAAVIGLTRQMAIELAQYGITVNS 184 Query: 184 VLPGIVAGARQERIVAAKATAYGIAHEEMRQRLLSKVSLRKMVTAEDVANQVIFICSPAG 243 V PG V + + E+ R+ V +R+ T +++A+ V ++ + Sbjct: 185 VAPGPVDTPMTQ----------AMHSEQTRESYYRLVPMRRYGTTDEIASAVCYLAAEES 234 Query: 244 ASISGRSLSVCG 255 A I+G + V G Sbjct: 235 AYITGHVIPVDG 246 >gi|300311568|ref|YP_003775660.1| toluenesulfonate Zinc-independent alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 252 Score = 111 bits (278), Expect = 5e-26, Method: Compositional matrix adjust. Identities = 81/255 (31%), Positives = 128/255 (50%), Gaps = 9/255 (3%) Query: 3 LNLKDQKVIVTAGAQGIGLAITAAFVEAGAHVHICDISEDFLASARERFGTA--PVSYSR 60 + L+D+ IVT G G G I A+ GA + + DI E E A +++ Sbjct: 1 MRLQDKVAIVTGGGSGFGEGIAKAYAREGAAIVVADIGEAGGLRVVEEIKAAGGRAVFAK 60 Query: 61 TDVSRASEVDAMFAELAQRWDGRLDVLVNNAGIAGPTLPVEEVDLSGWDQTIAVNLTGPF 120 DVS+ +++D + A + + G+LD++VNNAG P+ EV+ +D+ AVN+ F Sbjct: 61 ADVSKRADMDQLLATALEHF-GKLDIVVNNAGTTHRNRPMLEVEEDEFDRVYAVNVKSIF 119 Query: 121 LCTRRAVPMLKNNGGGAIVNISSVAGRLGFALRTPYSASKYGVIGLTETWAIELGPCNIR 180 L + VP + GGGA +NI+S AG T Y+ SK VI +++ A ELGP IR Sbjct: 120 LSAKTFVPYFRQVGGGAFINIASTAGIRPRPGLTWYNGSKGAVITTSKSMAAELGPDKIR 179 Query: 181 VNAVLPGIVAGARQERIVAAKATAYGIAHEEMRQRLLSKVSLRKMVTAEDVANQVIFICS 240 VN V P I A + T E R++ ++ + + + T ED+AN +++ S Sbjct: 180 VNCVNPVISATGLLSEFMGVPDTP------ENRKKFVATIPMGRFSTPEDIANACLYLGS 233 Query: 241 PAGASISGRSLSVCG 255 ++G + V G Sbjct: 234 DEAEFVTGVCIEVDG 248 >gi|300310595|ref|YP_003774687.1| 3-oxoacyl-(acyl-carrier protein) reductase [Herbaspirillum seropedicae SmR1] Length = 250 Score = 109 bits (273), Expect = 2e-25, Method: Compositional matrix adjust. Identities = 78/253 (30%), Positives = 125/253 (49%), Gaps = 13/253 (5%) Query: 4 NLKDQKVIVTAGAQGIGLAITAAFVEAGAHVHICDISEDFLASARERFGT-APVSYSRTD 62 + + + I+T GAQG G A+ ++ A V + D+ E LA AR + + V + D Sbjct: 5 DFQGRSAIITGGAQGFGYAVAERLLQGKAKVVLWDMDEKALAEARRKLESLGNVETVKVD 64 Query: 63 VSRASEVDAMFAELAQRWDGRLDVLVNNAGIAGPTLPVEEVDLSGWDQTIAVNLTGPFLC 122 +S +EV E ++ +D+LV++AGIAG PV E W + I ++L G F Sbjct: 65 ISSQAEVQQAI-EATEKLTQSIDILVHSAGIAGQNNPVAEYSPEEWRRVIDIDLNGAFYV 123 Query: 123 TRRAVPMLKNNGGGAIVNISSVAGRLGFALRTPYSASKYGVIGLTETWAIELGPCNIRVN 182 + V + G IVNI+S+AG+ G + YSA+K G+I LT++ E NI VN Sbjct: 124 NQVVVQRMIAQNYGRIVNIASIAGKEGNPTASAYSAAKAGMIALTKSLGKETATKNIAVN 183 Query: 183 AVLPGIVAGARQERIVAAKATAYGIAHEEMRQRLLSKVSLRKMVTAEDVANQVIFICSPA 242 A+ P A Q RI+ + T I + +LSK+ + V E++A V ++ S Sbjct: 184 AITP----AAAQTRIL-EQCTQAHIDY------MLSKIPRARFVKVEELAAMVAWLVSEE 232 Query: 243 GASISGRSLSVCG 255 + + + G Sbjct: 233 NSFTTASVFDLSG 245 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.134 0.388 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 792,364 Number of extensions: 32828 Number of successful extensions: 286 Number of sequences better than 1.0e-08: 47 Number of HSP's gapped: 195 Number of HSP's successfully gapped: 47 Length of query: 261 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 178 Effective length of database: 1,226,865 Effective search space: 218381970 Effective search space used: 218381970 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)