BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3386.p (586 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312730|ref|YP_003776822.1| methyl-accepting chemotaxis pro... 873 0.0 gi|300309376|ref|YP_003773468.1| methyl-accepting chemotaxis pro... 459 e-130 gi|300310693|ref|YP_003774785.1| methyl-accepting chemotaxis pro... 457 e-129 gi|300313820|ref|YP_003777912.1| methyl-accepting chemotaxis tra... 456 e-129 gi|300313938|ref|YP_003778030.1| methyl-accepting chemotaxis tra... 431 e-122 >gi|300312730|ref|YP_003776822.1| methyl-accepting chemotaxis protein [Herbaspirillum seropedicae SmR1] Length = 583 Score = 873 bits (2255), Expect = 0.0, Method: Compositional matrix adjust. Identities = 478/588 (81%), Positives = 517/588 (87%), Gaps = 7/588 (1%) Query: 1 MRFNNFSISTRLALAFSILILVIAAIVTLGLARLSDINQSLDLVVNDRYKKIAIINTISG 60 MRFNN SI+TRLA+AFSILILVIA IV LGLARL+DIN++LDLVVNDRYKKIAIINTISG Sbjct: 1 MRFNNLSIATRLAIAFSILILVIAGIVALGLARLADINKALDLVVNDRYKKIAIINTISG 60 Query: 61 KVDDVAISLRNELLMTNPEQIANEQKIIEALSAENTKLYDELTRLIADPEAKAKLTQVTE 120 KVDDVAISLRN+LLMT+P QIA EQK IE+LSAENTKLYDELTRL+A+PEAKAKL +VTE Sbjct: 61 KVDDVAISLRNQLLMTDPAQIAAEQKTIESLSAENTKLYDELTRLVANPEAKAKLDKVTE 120 Query: 121 TRKKYAAMRKEIDRLFTSGEKDAAIKLMLSQMVPLQKIYFGQLDELANMQQKLMDKSVEE 180 TRK YAA+RK+ID+L +SG+KDAAIKLMLS+MVPLQKIYF QLDEL++ QQKLMD+SV++ Sbjct: 121 TRKSYAALRKQIDKLQSSGDKDAAIKLMLSEMVPLQKIYFSQLDELSDTQQKLMDQSVDQ 180 Query: 181 AEATYLATRNIMLASGVLAALFAGFVAWTISRSITRPLSRAVEVAETVAAGDLTAVITAH 240 AEA Y TR IM+ +GVLAA FA F+AWTISRSITRPLSRAVEVAETVAAGDLTAVI AH Sbjct: 181 AEAAYETTRMIMVIAGVLAATFAAFIAWTISRSITRPLSRAVEVAETVAAGDLTAVIAAH 240 Query: 241 STDETGRLLQALAAMNQKLKAIVLEVRHSTDSMVTASTQIATGNLDLSSRTEEQASALEE 300 S DETGRLLQALAAMNQKLKAIVLEVR TDSMVTASTQIATGNLDLSSRTEEQASALEE Sbjct: 241 SNDETGRLLQALAAMNQKLKAIVLEVRRGTDSMVTASTQIATGNLDLSSRTEEQASALEE 300 Query: 301 TASSLEQLTSSVKQNADHTRKSSELAGTATAVANQGGEAVKQVVQTMGAINDSSRKIVDI 360 TASSLE+LTSSVKQNADHTRKSSELA A+ VANQGG AVKQVVQTMGAINDSS KIVDI Sbjct: 301 TASSLEELTSSVKQNADHTRKSSELAANASQVANQGGLAVKQVVQTMGAINDSSHKIVDI 360 Query: 361 ISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRALAQRSATAAKEIKELINDS 420 ISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVR LAQRSATAAKEIKELINDS Sbjct: 361 ISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRGLAQRSATAAKEIKELINDS 420 Query: 421 VNQVSSGTELVAQAGNTMDQVVNSIEQVGHIVGEISAATREQSDGIEQVNQAIMQMDNTT 480 VNQV+SGTELVAQAG+TMD VVNSIEQV HIVGEISAATREQSDGIEQVNQAIMQMDNTT Sbjct: 421 VNQVTSGTELVAQAGSTMDAVVNSIEQVSHIVGEISAATREQSDGIEQVNQAIMQMDNTT 480 Query: 481 QQNAALVEEAAAAAQALQDQAQRLLELVSVFRLDDAG--GPELRTMQAPQAAALKSSAVT 538 QQNAALVEEAAAAAQALQDQAQRLL+LV VFRLD+ G GP + AP A L+ Sbjct: 481 QQNAALVEEAAAAAQALQDQAQRLLDLVGVFRLDEVGGSGPAPSAV-APVAPVLRK---V 536 Query: 539 SKALPSKPQTQPQPQAQSARPAAIGANQAPRALPNSIKEDKKEEWESF 586 S A P ++ P + + A A ALP S +EDK EEWE+F Sbjct: 537 SAAAPRATKSLP-AKPAAPAVTNAPAAPAAPALPASKREDKSEEWETF 583 >gi|300309376|ref|YP_003773468.1| methyl-accepting chemotaxis protein I [Herbaspirillum seropedicae SmR1] Length = 534 Score = 459 bits (1180), Expect = e-130, Method: Compositional matrix adjust. Identities = 277/527 (52%), Positives = 371/527 (70%), Gaps = 1/527 (0%) Query: 1 MRFNNFSISTRLALAFSILILVIAAIVTLGLARLSDINQSLDLVVNDRYKKIAIINTISG 60 M+ +N +I RL L F +L++++ A+ G+ARL +N + V++D+Y K + I Sbjct: 1 MQLSNVTIRVRLWLGFGLLMVLMLAMAASGIARLGQLNAQMKDVIHDKYPKTVVAGDIVD 60 Query: 61 KVDDVAISLRNELLMTNPEQIANEQKIIEALSAENTKLYDELTRLIADPEAKAKLTQVTE 120 +++ +A S+RN LL+ QI +E + I + +L +L+ D +++A LT + Sbjct: 61 QLNLIARSVRNILLLKREPQIKSEYERISGADRAIREDLAQLDKLLTDGDSRAALTTLVT 120 Query: 121 TRKKYAAMRKEIDRLFTSGEKDAAIKLMLSQMVPLQKIYFGQLDELANMQQKLMDKSVEE 180 + Y R ++ + G K+ AI +++ ++ P+Q D L Q ++M + +E Sbjct: 121 AQNAYFEKRDKVLAMVQQGGKEGAIDMLIDEVRPVQSATMNAADALIRQQHEMMKTAGDE 180 Query: 181 AEATYLATRNIMLASGVLAALFAGFVAWTISRSITRPLSRAVEVAETVAAGDLTAVITAH 240 E+ Y R ++L A +AW I+RSIT PL RAV VAETVAAGDLT+ I AH Sbjct: 181 VESNYAQARTLLLLLAAAGLAAAAVIAWMITRSITAPLDRAVRVAETVAAGDLTSAIDAH 240 Query: 241 STDETGRLLQALAAMNQKLKAIVLEVRHSTDSMVTASTQIATGNLDLSSRTEEQASALEE 300 DET LL+AL MN +L IV +VR STD++ TAS++IA GNLDLSSRTEEQA++LEE Sbjct: 241 GRDETSMLLRALKKMNDRLLDIVSQVRVSTDTIATASSEIARGNLDLSSRTEEQAASLEE 300 Query: 301 TASSLEQLTSSVKQNADHTRKSSELAGTATAVANQGGEAVKQVVQTMGAINDSSRKIVDI 360 TASS+EQLT++VKQNA H ++S+LAG+A+ +A QGGE V VV+TMGAI+ SS+KIVDI Sbjct: 301 TASSMEQLTATVKQNASHAGQASQLAGSASDIARQGGEVVGHVVETMGAIDASSKKIVDI 360 Query: 361 ISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRALAQRSATAAKEIKELINDS 420 I VIDGIAFQTNILALNAAVEAARAGEQGRGFAVVA+EVRALAQRSATAA EIK LI DS Sbjct: 361 IGVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVAAEVRALAQRSATAAHEIKALIADS 420 Query: 421 VNQVSSGTELVAQAGNTMDQVVNSIEQVGHIVGEISAATREQSDGIEQVNQAIMQMDNTT 480 V +V +G++LV QAG TM +VV S+++VG +V EIS+A +EQS GIEQVN AI+QMD T Sbjct: 421 VARVGNGSKLVEQAGRTMHEVVQSVQRVGEVVNEISSAGQEQSAGIEQVNLAIVQMDQVT 480 Query: 481 QQNAALVEEAAAAAQALQDQAQRLLELVSVFRLDDAGGPELRTMQAP 527 QQNAALVE+AAAAAQALQDQA +L +VS+F+L+DA E R + AP Sbjct: 481 QQNAALVEQAAAAAQALQDQAAQLARMVSIFKLEDA-ASEPRRVAAP 526 >gi|300310693|ref|YP_003774785.1| methyl-accepting chemotaxis protein [Herbaspirillum seropedicae SmR1] Length = 576 Score = 457 bits (1175), Expect = e-129, Method: Compositional matrix adjust. Identities = 287/562 (51%), Positives = 382/562 (67%), Gaps = 11/562 (1%) Query: 1 MRFNNFSISTRLALAFSILILVIAAIVTLGLARLSDINQSLDLVVNDRYKKIAIINTISG 60 M+ ++ IS RL F+ LI+V+ I +G+ L ++ + D +VNDRY K+A+ I+ Sbjct: 2 MKISDMKISVRLGACFAALIVVMCVIAGVGVKNLLMVSDATDEIVNDRYVKVALGTQITD 61 Query: 61 KVDDVAISLRNELLMTNPEQIANEQKIIEALSAENTKLYDELTRLIADPEAKAKLTQVTE 120 KV+ A SLRN ++ NPE+ ++ +++ ++ E+ + P L + + Sbjct: 62 KVNTGARSLRNAIMARNPEEQKKYLDRMDTYTSQVSEHLAEMEKRTNTPRGSELLAALKQ 121 Query: 121 TRKKYAAMRKEIDRLFTSGEKDAAIKLMLSQMVPLQKIYFGQLDELANMQQKLMDKSVEE 180 R Y+ R ++ L ++D A + + +P+Q YF + E+ + Q LMDKS E Sbjct: 122 ARADYSKERIKVVDLIHQQKRDEATAHLFDKTIPVQDKYFAAVKEMVSFQSSLMDKSAER 181 Query: 181 AEATYLATRNIMLASGVLAALFAGFVAWTISRSITRPLSRAVEVAETVAAGDLTAVITAH 240 A A + NIML +A L + A I+RSITRPL+ AV+VA VAAGDLT I A Sbjct: 182 ASAASSSAINIMLVLSGVAVLLSALCAMLITRSITRPLNEAVQVATAVAAGDLTVQIEAT 241 Query: 241 STDETGRLLQALAAMNQKLKAIVLEVRHSTDSMVTASTQIATGNLDLSSRTEEQASALEE 300 S DETG LL AL +MN L IV EVR TD++ TAS +IATGNLDLSSRTE+QA ALEE Sbjct: 242 SKDETGALLGALKSMNDNLHRIVAEVRAGTDTINTASREIATGNLDLSSRTEQQAGALEE 301 Query: 301 TASSLEQLTSSVKQNADHTRKSSELAGTATAVANQGGEAVKQVVQTMGAINDSSRKIVDI 360 TAS++E+LTS+V+QNAD+ R+++ LA TA+ VA QGG V +VVQTMG IND+SRKIVDI Sbjct: 302 TASAMEELTSTVRQNADNARQANTLAETASQVAIQGGSVVGEVVQTMGQINDASRKIVDI 361 Query: 361 ISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRALAQRSATAAKEIKELINDS 420 ISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVR LAQRSA AAKEIK LI+ S Sbjct: 362 ISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRTLAQRSAAAAKEIKSLIDAS 421 Query: 421 VNQVSSGTELVAQAGNTMDQVVNSIEQVGHIVGEISAATREQSDGIEQVNQAIMQMDNTT 480 V +V SG+ LV QAG TM++VV S+++V +V EI+AA+ EQSDGIEQ+NQAI+QMD T Sbjct: 422 VERVESGSRLVGQAGATMEEVVASVKRVTDVVAEITAASGEQSDGIEQINQAIVQMDEVT 481 Query: 481 QQNAALVEEAAAAAQALQDQAQRLLELVSVFRL--DDAGGPELRTMQAPQAAALKSSAVT 538 QQNAALVE+AAAAAQ+LQ+Q+ RL + V +FRL D A P +P +A ++ S Sbjct: 482 QQNAALVEQAAAAAQSLQEQSGRLSDTVGIFRLGNDHAHQP------SPLSAPVQRSV-- 533 Query: 539 SKALPSKPQTQPQPQAQSARPA 560 + P++P +P +++ PA Sbjct: 534 -QVAPARPVLARKPVTRTSAPA 554 >gi|300313820|ref|YP_003777912.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 597 Score = 456 bits (1172), Expect = e-129, Method: Compositional matrix adjust. Identities = 283/513 (55%), Positives = 360/513 (70%) Query: 1 MRFNNFSISTRLALAFSILILVIAAIVTLGLARLSDINQSLDLVVNDRYKKIAIINTISG 60 M+ + IS RLA+ F++LI V+ I +G+ LS I++S L+V DRY+KIA+ I Sbjct: 1 MKLKDIKISVRLAVCFAVLIGVMCLITGVGMKSLSSISKSSALIVEDRYEKIALAVEIRD 60 Query: 61 KVDDVAISLRNELLMTNPEQIANEQKIIEALSAENTKLYDELTRLIADPEAKAKLTQVTE 120 V+ A +LRN LL N E+ A SA+ T+ ++ ++++ + + E Sbjct: 61 NVNGAARNLRNALLGRNLEEATRYLDRSAANSAKTTEALGKMEKILSTARGRELFQSIQE 120 Query: 121 TRKKYAAMRKEIDRLFTSGEKDAAIKLMLSQMVPLQKIYFGQLDELANMQQKLMDKSVEE 180 R Y R ++ L +K+ A++L+ +Q++P Q YF LD+ Q+ LM++SV E Sbjct: 121 ARSAYNTPRDKLRELIKQQKKEEAVELLFTQVIPAQDKYFQVLDQFVAFQKSLMEQSVTE 180 Query: 181 AEATYLATRNIMLASGVLAALFAGFVAWTISRSITRPLSRAVEVAETVAAGDLTAVITAH 240 T + +ML +A AW ++RSITRPL+ AV VA VAAGDLT I A Sbjct: 181 GRDTTQSAIAMMLGLSAVAIALCVIAAWLVTRSITRPLNEAVSVASAVAAGDLTVQIGAT 240 Query: 241 STDETGRLLQALAAMNQKLKAIVLEVRHSTDSMVTASTQIATGNLDLSSRTEEQASALEE 300 S DETG LL AL +MN L IV EVR +D++ TAS +IATGNLDLSSRTEEQA ALEE Sbjct: 241 SKDETGALLAALKSMNDSLHRIVSEVRQGSDTINTASAEIATGNLDLSSRTEEQAGALEE 300 Query: 301 TASSLEQLTSSVKQNADHTRKSSELAGTATAVANQGGEAVKQVVQTMGAINDSSRKIVDI 360 TAS++E+LTS+VKQNAD+ R+++ LA TA+ VA QGG V QVVQTMG IND+SRKIVDI Sbjct: 301 TASAMEELTSTVKQNADNARQANSLAATASEVAVQGGSVVGQVVQTMGEINDASRKIVDI 360 Query: 361 ISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRALAQRSATAAKEIKELINDS 420 ISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVR LAQRSA+AAKEIK LI+DS Sbjct: 361 ISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRTLAQRSASAAKEIKALIDDS 420 Query: 421 VNQVSSGTELVAQAGNTMDQVVNSIEQVGHIVGEISAATREQSDGIEQVNQAIMQMDNTT 480 V++V +G+ LV QAG TM +VV S+ +V +V EISAA+ EQSDGIEQ+N AI QMD T Sbjct: 421 VSRVDNGSRLVEQAGTTMTEVVASVRRVTDVVAEISAASNEQSDGIEQINHAITQMDQVT 480 Query: 481 QQNAALVEEAAAAAQALQDQAQRLLELVSVFRL 513 QQNAALVEEAAAAAQ+LQ+Q+ RL VSVF+L Sbjct: 481 QQNAALVEEAAAAAQSLQEQSTRLSATVSVFKL 513 >gi|300313938|ref|YP_003778030.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 533 Score = 431 bits (1109), Expect = e-122, Method: Compositional matrix adjust. Identities = 264/518 (50%), Positives = 355/518 (68%), Gaps = 10/518 (1%) Query: 3 FNNFSISTRLALAFSILILVIAAIVTLGLARLSDINQSLDLVVNDRYKKIAIINTISGKV 62 N I RLA+ F L++++A +V LG+ RL I + D +IA+ Sbjct: 1 MKNLKIGVRLAIGFIALLMLMAVMVALGVWRLGSIGDA-----TDDMTRIALKKERDAVQ 55 Query: 63 DDVAI---SLRNELLMTNPEQI--ANEQKIIEALSAENTKLYDELTRLIADPEAKAKLTQ 117 AI +R +M + + A QK I+A SA ++L E+ I D E K + Sbjct: 56 WHAAIKENGVRTFAVMKSDDDAFQAYFQKQIDAQSARISQLQKEVEAAITDAEEKRLFDE 115 Query: 118 VTETRKKYAAMRKEIDRLFTSGEKDAAIKLMLSQMVPLQKIYFGQLDELANMQQKLMDKS 177 V + R Y A R+ I + T+G+ A ++ + +V + Y + A+ Q++++D++ Sbjct: 116 VGKLRAAYRATRQSIFDVKTAGDDARAREMTDTTLVKMMDDYADGVLRYADFQKQVIDQT 175 Query: 178 VEEAEATYLATRNIMLASGVLAALFAGFVAWTISRSITRPLSRAVEVAETVAAGDLTAVI 237 Y + R+++LA G + + +AW ++RSITRPL++AV +AETVAAGDLT+ I Sbjct: 176 AAAINRDYRSGRSMLLALGAIEIVLGAALAWLLTRSITRPLNQAVTIAETVAAGDLTSRI 235 Query: 238 TAHSTDETGRLLQALAAMNQKLKAIVLEVRHSTDSMVTASTQIATGNLDLSSRTEEQASA 297 + S DETG+LL AL MN L IV VR T+++ TAS +IATGNLDLS+RTE QA + Sbjct: 236 DSSSRDETGKLLAALKTMNGNLLDIVGRVRMGTETINTASREIATGNLDLSARTEAQAGS 295 Query: 298 LEETASSLEQLTSSVKQNADHTRKSSELAGTATAVANQGGEAVKQVVQTMGAINDSSRKI 357 LEETAS++EQLTS+VKQNAD+ R+++ELA +A+ VA QGG V QVV TMGAI+ SS+KI Sbjct: 296 LEETASAMEQLTSTVKQNADNARQANELAASASQVATQGGAVVDQVVATMGAIHASSQKI 355 Query: 358 VDIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRALAQRSATAAKEIKELI 417 DII VIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVR+LAQRSA+AAKEIK+LI Sbjct: 356 ADIIGVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSASAAKEIKQLI 415 Query: 418 NDSVNQVSSGTELVAQAGNTMDQVVNSIEQVGHIVGEISAATREQSDGIEQVNQAIMQMD 477 +DSV +V G+ LVA+AG+TM +VV S+ +V +V EI+ A++EQSDGIEQVN AI +D Sbjct: 416 DDSVEKVDDGSRLVARAGDTMQEVVQSVARVTAVVREITLASQEQSDGIEQVNLAITHID 475 Query: 478 NTTQQNAALVEEAAAAAQALQDQAQRLLELVSVFRLDD 515 TTQQNAALVE+AAAAA+++QDQA L + VSVFRL+D Sbjct: 476 ETTQQNAALVEQAAAAARSMQDQAASLTDAVSVFRLED 513 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.311 0.124 0.316 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,206,060 Number of extensions: 37000 Number of successful extensions: 637 Number of sequences better than 1.0e-08: 71 Number of HSP's gapped: 304 Number of HSP's successfully gapped: 72 Length of query: 586 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 496 Effective length of database: 1,193,720 Effective search space: 592085120 Effective search space used: 592085120 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 133 (55.8 bits)