BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3376.p (260 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312720|ref|YP_003776812.1| enoyl-[acyl-carrier-protein] re... 518 e-148 gi|300311721|ref|YP_003775813.1| NADH-dependent enoyl-[acyl-carr... 206 2e-54 gi|300313037|ref|YP_003777129.1| short-chain dehydrogenase [Herb... 77 2e-15 gi|300313745|ref|YP_003777837.1| short-chain alcohol dehydrogena... 65 8e-12 gi|300310400|ref|YP_003774492.1| 2-deoxy-D-gluconate 3-dehydroge... 57 1e-09 >gi|300312720|ref|YP_003776812.1| enoyl-[acyl-carrier-protein] reductase [Herbaspirillum seropedicae SmR1] Length = 260 Score = 518 bits (1334), Expect = e-148, Method: Compositional matrix adjust. Identities = 258/260 (99%), Positives = 259/260 (99%) Query: 1 MAFLQGKKILITGLLSNRSIAYGIAQACKREGAELAFTYVGERFKERITKFAEEFDSKLV 60 MAFLQGKKILITGLLSNRSIAYGIAQACKREGAELAFTYVGERFK+RITKFAEEFDSKLV Sbjct: 1 MAFLQGKKILITGLLSNRSIAYGIAQACKREGAELAFTYVGERFKDRITKFAEEFDSKLV 60 Query: 61 FDCDVSSDEQINALFADLGKSWSQLDGLVHAIGFAPSEAIAGDFLEGFSREGFKIAHDIS 120 FDCDV SDEQINALFADLGKSWSQLDGLVHAIGFAPSEAIAGDFLEGFSREGFKIAHDIS Sbjct: 61 FDCDVGSDEQINALFADLGKSWSQLDGLVHAIGFAPSEAIAGDFLEGFSREGFKIAHDIS 120 Query: 121 AYSFPAMAKAAMPLLSSNAALLTLTYLGSERVVPNYNTMGLAKASLEASVRYLAESLGPK 180 AYSFPAMAKAAMPLLSSNAALLTLTYLGSERVVPNYNTMGLAKASLEASVRYLAESLGPK Sbjct: 121 AYSFPAMAKAAMPLLSSNAALLTLTYLGSERVVPNYNTMGLAKASLEASVRYLAESLGPK 180 Query: 181 GVRVNGVSAGPIKTLAASGIKGFGKILGAVAATAPLRRNVTIEDVGNASAFLLSDLASGI 240 GVRVNGVSAGPIKTLAASGIKGFGKILGAVAATAPLRRNVTIEDVGNASAFLLSDLASGI Sbjct: 181 GVRVNGVSAGPIKTLAASGIKGFGKILGAVAATAPLRRNVTIEDVGNASAFLLSDLASGI 240 Query: 241 TGEITYVDGGFSRVVGGMAE 260 TGEITYVDGGFSRVVGGMAE Sbjct: 241 TGEITYVDGGFSRVVGGMAE 260 >gi|300311721|ref|YP_003775813.1| NADH-dependent enoyl-[acyl-carrier-protein] reductase [Herbaspirillum seropedicae SmR1] Length = 255 Score = 206 bits (523), Expect = 2e-54, Method: Compositional matrix adjust. Identities = 106/251 (42%), Positives = 158/251 (62%), Gaps = 1/251 (0%) Query: 4 LQGKKILITGLLSNRSIAYGIAQACKREGAELAFTYVGERFKERITKFAEEFDSKLVFDC 63 L+GK+ LI G+ SIA+G A+A + GA+LA T++ E + + A++ + ++ Sbjct: 5 LEGKRGLIVGIADKHSIAWGCARALRAAGADLAVTWLNESARRYVEPLADQVQASIMCGL 64 Query: 64 DVSSDEQINALFADLGKSWSQLDGLVHAIGFAPSEAIAGDFLEGFSREGFKIAHDISAYS 123 DV+ ++ +F + W LD ++H+I FAP+ + G + S +GF +A D+S +S Sbjct: 65 DVTHPGEMEQVFDLIRTHWGSLDFVLHSIAFAPAPDLHGR-VTNCSSQGFSLAMDVSCHS 123 Query: 124 FPAMAKAAMPLLSSNAALLTLTYLGSERVVPNYNTMGLAKASLEASVRYLAESLGPKGVR 183 F MA+ A PL+ + +LLT++Y+G+E VV NY MG KA+LE++VRYLA LGP G+R Sbjct: 124 FIRMARLAEPLMKNGGSLLTMSYIGAEEVVVNYGVMGPVKAALESAVRYLAMELGPAGIR 183 Query: 184 VNGVSAGPIKTLAASGIKGFGKILGAVAATAPLRRNVTIEDVGNASAFLLSDLASGITGE 243 VN VS GP+ T AASG+ F + L A +PLRR I+DVG+ +FL+SD AS ITG Sbjct: 184 VNAVSPGPLATRAASGLNCFEQFLTDAARRSPLRRLANIDDVGDMCSFLVSDAASVITGN 243 Query: 244 ITYVDGGFSRV 254 YVD G+ V Sbjct: 244 TLYVDAGYHIV 254 >gi|300313037|ref|YP_003777129.1| short-chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 369 Score = 77.0 bits (188), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 80/260 (30%), Positives = 122/260 (46%), Gaps = 31/260 (11%) Query: 4 LQGKKILITGLLSNRSIAYGIAQACKREGAELAFTYVGERFKERIT-KFAEEFDSK-LVF 61 L GK LITG + I +A A REGA LA Y+ E R T K + D+ L+ Sbjct: 123 LAGKVALITG--GDSGIGRAVALAYAREGARLAIVYLDEEEDARQTQKDVQALDAPCLLL 180 Query: 62 DCDVSSDEQINALFADLGKSWSQLDGLVH-AIGFAPSEAIAGDFLEGFSREGFKIAHDIS 120 DV S E + + ++ +LD LV+ A P+++I E S+ + + Sbjct: 181 PGDVGSPEFCRSAVRAVMDAYGRLDVLVNNAAQQYPAQSI-----EEISQAQLEHTFRTN 235 Query: 121 AYSFPAMAKAAMPLLSSNAALLTLT----YLGSERVVPNYNTMGLAKASLEASVRYLAES 176 +S M K AMP L+ A ++ T Y GS+ +V +Y K ++ A R L++ Sbjct: 236 IFSMFYMVKEAMPFLTRGARIINTTSVTAYRGSKHLV-DY---AATKGAIVAFTRSLSQQ 291 Query: 177 LGPKGVRVNGVSAGPIKT------LAASGIKGFGKILGAVAATAPLRRNVTIEDVGNASA 230 L G+ VN V+ GPI T +A + FGK PL R ++V A Sbjct: 292 LASAGIHVNAVAPGPIWTPLIPASFSAEEVASFGK-------NVPLGRPGQPDEVAPAYV 344 Query: 231 FLLSDLASGITGEITYVDGG 250 FL ++ AS +TG++ + +GG Sbjct: 345 FLATEGASYMTGQVLHPNGG 364 >gi|300313745|ref|YP_003777837.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 64.7 bits (156), Expect = 8e-12, Method: Compositional matrix adjust. Identities = 73/262 (27%), Positives = 113/262 (43%), Gaps = 28/262 (10%) Query: 4 LQGKKILITGLLSNRSIAYGIAQACKREGAELAFTYVGERFKERITKFAEEFDSKLVFDC 63 L GK L+TG S I AQ +GA++ T G R E A + Sbjct: 5 LNGKIALVTGGTSG--IGLATAQELAAQGAQVFIT--GRRQAELDAAVASIGAAATGIRA 60 Query: 64 DVSSDEQINALFADLGKSWSQLDGLVHAIGFAPSEAIAGDF--LEGFSREGFKIAHDISA 121 D S +++ ++A + KS +LD L G GD L + E F + Sbjct: 61 DASVLSELDTVYAQIAKSAGRLDILFANAG-------GGDMMPLGAITEEHFDRIFGTNV 113 Query: 122 YSFPAMAKAAMPLLSSNAALLTLTYLGSERVVPNYNTMGLAKASLEASVRYLAESLGPKG 181 + A+PLL A+++ + S N++ +KA++ R A L + Sbjct: 114 RGVLFTVQKALPLLVDGASVILTSSTTSVLGTANFSVYSASKAAVRNFARSWALDLKDRA 173 Query: 182 VRVNGVSAGPIKTLAASGIKGFGKIL---------GAVAATAPLRRNVTIEDVGNASAFL 232 +RVN VS GPI+T G G+++ +A+ PL R +++G A AFL Sbjct: 174 IRVNVVSPGPIRT------PGLGELVPDDARQGLFDYLASQVPLGRLGEPQEIGKAVAFL 227 Query: 233 LSDLASGITGEITYVDGGFSRV 254 SD AS + G +VDGG ++V Sbjct: 228 ASDAASFVNGIELFVDGGMAQV 249 >gi|300310400|ref|YP_003774492.1| 2-deoxy-D-gluconate 3-dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 241 Score = 57.4 bits (137), Expect = 1e-09, Method: Compositional matrix adjust. Identities = 69/249 (27%), Positives = 108/249 (43%), Gaps = 18/249 (7%) Query: 6 GKKILITGLLSNRSIAYGIAQACKREGAELAFTYVGERFKERITKFAEEFDSKLVFDCDV 65 GK ++ITG + R I A + GA++ R + + EE ++ V D+ Sbjct: 5 GKTVIITG--AGRGIGRACALLLAQRGAQVIAL---ARTASDLARLQEEIGAR-VIAVDL 58 Query: 66 SSDEQINALFADLGKSWSQLDGLVHAIGFAPSEAIAGDFLEGFSREGFK--IAHDISAYS 123 S A+ G + D L+++ G E++ LE S EG++ + ++ A Sbjct: 59 SDPAAARRAMAEAGTA----DFLINSAGINVLESV----LE-MSDEGYEAVLGVNLRAAL 109 Query: 124 FPAMAKAAMPLLSSNA-ALLTLTYLGSERVVPNYNTMGLAKASLEASVRYLAESLGPKGV 182 A A M + A++ +T + R + +KA LE + R LA LGP G+ Sbjct: 110 ITCQAFARMRIAQGGGGAMVNITSIAGHRGFEQHLCYAASKAGLEGATRVLARELGPHGI 169 Query: 183 RVNGVSAGPIKTLAASGIKGFGKILGAVAATAPLRRNVTIEDVGNASAFLLSDLASGITG 242 RVN V+ T A G + A P R + EDV + A LLS A+ + G Sbjct: 170 RVNAVAPTITLTELAEAAWSDPAKSGPMMARHPSARFASPEDVARSIAMLLSPDAAMLNG 229 Query: 243 EITYVDGGF 251 + VDGGF Sbjct: 230 AVVPVDGGF 238 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.135 0.377 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 742,021 Number of extensions: 29436 Number of successful extensions: 175 Number of sequences better than 1.0e-08: 17 Number of HSP's gapped: 178 Number of HSP's successfully gapped: 17 Length of query: 260 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 177 Effective length of database: 1,226,865 Effective search space: 217155105 Effective search space used: 217155105 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)