BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3363.p (833 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312698|ref|YP_003776790.1| hypothetical protein Hsero_3401... 1389 0.0 gi|300312704|ref|YP_003776796.1| transmembrane protein [Herbaspi... 1271 0.0 gi|300313107|ref|YP_003777199.1| transmembrane protein [Herbaspi... 1081 0.0 gi|300310085|ref|YP_003774177.1| hypothetical protein Hsero_0750... 64 5e-11 >gi|300312698|ref|YP_003776790.1| hypothetical protein Hsero_3401 [Herbaspirillum seropedicae SmR1] Length = 829 Score = 1389 bits (3595), Expect = 0.0, Method: Compositional matrix adjust. Identities = 657/828 (79%), Positives = 727/828 (87%) Query: 1 MNGCNFCDKKGLLIYPVRYAVASPYGAAGVPGLSGHFKIEGAPQAVGAAKYTLRALRAGY 60 MNGCNFCDK GLLIYPVRYAVASPYGA GVPGLSG+FKIEGAPQ+ GAAKY+LRALR GY Sbjct: 1 MNGCNFCDKNGLLIYPVRYAVASPYGATGVPGLSGNFKIEGAPQSAGAAKYSLRALRPGY 60 Query: 61 LYTYDEKRGRLKAYVVMPTGHLWNYPLEYKPPHPAKIRFACVDTGELVRAYCVDIIHTAA 120 LYTYDEKR RLKAYVVMPTGHLW+YPLEY PPHPAK+RFAC D GELVRAYCVDI HTA+ Sbjct: 61 LYTYDEKRRRLKAYVVMPTGHLWHYPLEYNPPHPAKLRFACTDPGELVRAYCVDIPHTAS 120 Query: 121 DPAGNFWIGWSNVQWTKALLKKVSDAGWRKKHMQCIDIAAMLAGSAAHTGEFNASMDKVA 180 DPAGNFWIGWSNVQWTKALLKKVSDAGWRKKHMQCIDI AMLAGSA HTGEFNAS DKVA Sbjct: 121 DPAGNFWIGWSNVQWTKALLKKVSDAGWRKKHMQCIDIPAMLAGSAVHTGEFNASADKVA 180 Query: 181 HFAADNTALQKAFSFSNTPSRSEPSKQAAAPHFTAIMAKHEPHHQGFIAAINDPVGMAND 240 HF AD A++KA++FSNTP SE K A A F AIMA+H PHH+G+IAA+NDPVGM ND Sbjct: 181 HFVADEAAMKKAYAFSNTPIESETRKLATAVRFKAIMAEHGPHHKGYIAALNDPVGMTND 240 Query: 241 LSELTMPDLLAGFDEKLHQRKMIADLLALTEHHVRQEARQEVVFSDAVAEASAHHPDGDV 300 SELTMPDL AGFDEKLHQRKMIADLLALTEHHVRQEARQEVVFSDAVAEASAHHPDGD+ Sbjct: 241 WSELTMPDLQAGFDEKLHQRKMIADLLALTEHHVRQEARQEVVFSDAVAEASAHHPDGDI 300 Query: 301 YNGLKTLGAMFKLGGINRHEKKLQEQRKKYGEDLAGRQQAAADDAWHDLTHDNGKPTLDE 360 YNGLKTLGAMFKLGGI+RHEKKLQEQRKKYGEDLAGRQQAAADDAWH+LTHDNGKPTLDE Sbjct: 301 YNGLKTLGAMFKLGGIDRHEKKLQEQRKKYGEDLAGRQQAAADDAWHELTHDNGKPTLDE 360 Query: 361 AALKAFPAIYDAALKQFEPQLLQLLMAHVGWLKSEQLANWMEGVHDDADIRSGYAYSESM 420 AA+KAFPAIYDAALKQFEPQLLQLLMAH+GWLKSEQLANWMEGVHDDADIRSGYAYSESM Sbjct: 361 AAMKAFPAIYDAALKQFEPQLLQLLMAHLGWLKSEQLANWMEGVHDDADIRSGYAYSESM 420 Query: 421 SQCIGKAACSKPCIEQLMRWISSDNLKDTRNLYGRALLLNQADIIAATEVQLKGSDIQIE 480 SQCIGKA CSKPCI+QL WISS NL+D RNLY RALLLNQADIIAATE Q KGSD Q+E Sbjct: 421 SQCIGKAVCSKPCIDQLASWISSGNLQDKRNLYVRALLLNQADIIAATETQFKGSDFQLE 480 Query: 481 NILNIYKGAIERLHNAGHAEQLIDRLALTTGNVIAKAISESGSLLAKGLAQIHLQLMAGV 540 NIL++YKGA+ RL + AEQLIDRLALTTGNVI KAIS S S LA+ +A HLQL+ GV Sbjct: 481 NILHVYKGAVMRLDDPKQAEQLIDRLALTTGNVIVKAISGSSSALARAIALTHLQLLGGV 540 Query: 541 AIKVSNMSSADVARWAIAEAKEKGIKLETNRQKTRADARTEAGRAIKRAKVEKHVVYELD 600 IKVSNMS DVARWAIA+AKE+GI+LET+R++TR DAR +A +A+K+A+V+ H Y LD Sbjct: 541 GIKVSNMSRTDVARWAIAQAKEQGIQLETDRKETRVDARNQARKAVKQARVQNHFFYVLD 600 Query: 601 IARLEKEGRIAPGSIKGIGLPGFAMTQKWLGSGAPRDFKLGVVTMIVQMVALNFAMKDLV 660 I LEK+GRIAPGSIKGIG+PG + WLGSG+PRDFKLGV+TMIVQM AL FAM DL Sbjct: 601 IGALEKDGRIAPGSIKGIGIPGVTTAKNWLGSGSPRDFKLGVMTMIVQMAALQFAMTDLD 660 Query: 661 GNDQFNQVETRVKTTLAIISLSATIVETAAASLQKSVEHPLAVFIRGQWAIEKKWMSTLL 720 NDQFNQ ETR K LAIISLSAT+VE A S++KSVEHPLAVFIR QWA++ + +ST+L Sbjct: 661 NNDQFNQWETRTKAALAIISLSATMVEIAMVSVEKSVEHPLAVFIRNQWAVDAELVSTIL 720 Query: 721 KGARMAGAGAGILAGLFDLAFNAFPAYRDENYLLMTLYGLNGLSGIGIAFAAYYSIAAVW 780 K AR AG AG+LA L+DL FNA ++DENYLLM LY LNG+ GIGIA AAYY+I ++W Sbjct: 721 KRARKAGMVAGVLAELYDLVFNAPQEFKDENYLLMALYVLNGILGIGIALAAYYAITSIW 780 Query: 781 TPLLIVSFAISVSIAMVNNSQLKTWISRCDFSSKEKYSSFEEQINAFN 828 PLL++SFA+ +++A+VNNSQLKTWISRCDFS EKYSSFE+QI AFN Sbjct: 781 APLLLISFAVGIALALVNNSQLKTWISRCDFSLGEKYSSFEDQIKAFN 828 >gi|300312704|ref|YP_003776796.1| transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 837 Score = 1271 bits (3288), Expect = 0.0, Method: Compositional matrix adjust. Identities = 603/837 (72%), Positives = 698/837 (83%), Gaps = 4/837 (0%) Query: 1 MNGCNFCDKKGLLIYPVRYAVASPYGAAGVPGLSGHFKIEGAPQAVGAAKYTLRALRAGY 60 MNGCNFCDKKGLLIYPVRYAVASPYGAAGVPGLSG+FKIEGAPQ+ GAAKY+LRALR GY Sbjct: 1 MNGCNFCDKKGLLIYPVRYAVASPYGAAGVPGLSGNFKIEGAPQSAGAAKYSLRALRPGY 60 Query: 61 LYTYDEKRGRLKAYVVMPTGHLWNYPLEYKPPHPAKIRFACVDTGELVRAYCVDIIHTAA 120 LYTYDEKRGRLKAYVVMPTGHLW+YPLEY PPHPAK+RFAC D GELVRAYCVDI HTA+ Sbjct: 61 LYTYDEKRGRLKAYVVMPTGHLWHYPLEYNPPHPAKLRFACTDPGELVRAYCVDIPHTAS 120 Query: 121 DPAGNFWIGWSNVQWTKALLKKVSDAGWRKKHMQCIDIAAMLAGSAAHTGEFNASMDKVA 180 DPAGNFWIGWSNVQWTKALLKKVSDAGWRKKHMQCIDI AMLAGSA HTGEFNAS DKVA Sbjct: 121 DPAGNFWIGWSNVQWTKALLKKVSDAGWRKKHMQCIDIPAMLAGSAVHTGEFNASADKVA 180 Query: 181 HFAADNTALQKAFSFSNTPSRSEPSKQAAAPHFTAIMAKHEPHHQGFIAAINDPVGMAND 240 HF AD A++KA++FSNTP SE K A A F AIMA+H PHH+G+IAA+NDPVGM ND Sbjct: 181 HFVADEAAMKKAYAFSNTPIESETRKLATAVRFKAIMAEHGPHHKGYIAALNDPVGMTND 240 Query: 241 LSELTMPDLLAGFDEKLHQRKMIADLLALTEHHVRQEARQEVVFSDAVAEASAHHPDGDV 300 LSELTMPDL AGFDEK+HQ KMIADLL +TE VR++AR++V FSD VAE SA+HPDGD Sbjct: 241 LSELTMPDLQAGFDEKMHQSKMIADLLTITEQSVRKQAREDVEFSDKVAEMSANHPDGDT 300 Query: 301 YNGLKTLGAMFKLGGINRHEKKLQEQRKKYGEDLAGRQQAAADDAWHDLTHDNGKPTLDE 360 YN KT+ AM K GG R+ +K +E+RKKYGED AGR QAA D+AW+DLTHDN KPTLD Sbjct: 301 YNSAKTVFAMIKAGGPGRYAEKQREKRKKYGEDQAGRMQAAEDEAWYDLTHDNDKPTLDM 360 Query: 361 AALKAFPAIYDAALKQFEPQLLQLLMAHVGWLKSEQLANWMEGVHDDADIRSGYAYSESM 420 ++ FPA Y+AAL++FEP+ LQL+MAH+GWLKSEQLANWMEGVHDDADIRSGYAYSESM Sbjct: 361 KRIEEFPAQYEAALREFEPRFLQLVMAHLGWLKSEQLANWMEGVHDDADIRSGYAYSESM 420 Query: 421 SQCIGKAACSKPCIEQLMRWISSDNLKDTRNLYGRALLLNQADIIAATEVQLKGSDIQIE 480 SQCIGKAACSKPCIEQL RWISSD+LKD RNLYGRALL NQADIIAATE QLKGSDIQ E Sbjct: 421 SQCIGKAACSKPCIEQLTRWISSDDLKDRRNLYGRALLFNQADIIAATETQLKGSDIQFE 480 Query: 481 NILNIYKGAIERLHNAGHAEQLIDRLALTTGNVIAKAISESGSLLAKGLAQIHLQLMAGV 540 NILN+YKGA+ERLH+ EQLIDRLALTTGNV++KAISE+ ++LA+GL +IHL L+ G Sbjct: 481 NILNVYKGAVERLHDPMIEEQLIDRLALTTGNVVSKAISENSNVLARGLGRIHLHLLGGA 540 Query: 541 AIKVSNMSSADVARWAIAEAKEKGIKLETNRQKTRADARTEAGRA---IKRAKV-EKHVV 596 AIK+S+M S DV WA+++A+E+GIKLET RQ+TRADAR E R +K AKV ++ + Sbjct: 541 AIKISDMRSEDVVHWALSQAREQGIKLETRRQQTRADARQEVARVRKEVKEAKVGQRFIA 600 Query: 597 YELDIARLEKEGRIAPGSIKGIGLPGFAMTQKWLGSGAPRDFKLGVVTMIVQMVALNFAM 656 YELDIA+LEKEGRIAPGSIKGIGLPG AM QKWLGSG+PRDFKLGVVTMIVQMVAL FA Sbjct: 601 YELDIAKLEKEGRIAPGSIKGIGLPGVAMAQKWLGSGSPRDFKLGVVTMIVQMVALTFAT 660 Query: 657 KDLVGNDQFNQVETRVKTTLAIISLSATIVETAAASLQKSVEHPLAVFIRGQWAIEKKWM 716 KDL NDQFN++ETR+K TLAI+SL+ATIVE AA S ++EHPL FIR QWA+ +K + Sbjct: 661 KDLANNDQFNRLETRLKVTLAIVSLTATIVEIAAVSADPAMEHPLGAFIRRQWAVSEKAL 720 Query: 717 STLLKGARMAGAGAGILAGLFDLAFNAFPAYRDENYLLMTLYGLNGLSGIGIAFAAYYSI 776 ++KGAR GA AG++AG +D+ N+ A + + L LY +NG+ GI + A Y+SI Sbjct: 721 ERIIKGARFFGALAGVVAGFYDILVNSSNANKAGDERLSDLYFINGILGIVLPAAIYFSI 780 Query: 777 AAVWTPLLIVSFAISVSIAMVNNSQLKTWISRCDFSSKEKYSSFEEQINAFNQIFGG 833 A+ PL I SF + ++IA N S LK W++RC+FS+ EKY+SFE Q+ AFN GG Sbjct: 781 GAIAWPLFIFSFVVGIAIAYYNASALKNWVARCEFSTGEKYNSFEMQLKAFNDATGG 837 >gi|300313107|ref|YP_003777199.1| transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 833 Score = 1081 bits (2796), Expect = 0.0, Method: Compositional matrix adjust. Identities = 525/828 (63%), Positives = 639/828 (77%), Gaps = 2/828 (0%) Query: 1 MNGCNFCDKKGLLIYPVRYAVASPYGAAGVPGLSGHFKIEGAPQAVGAAKYTLRALRAGY 60 M CN+CDK+GLLIYPVRYAVASPYGA VP LSG+F+IEGAPQ +G A+YTLRALR GY Sbjct: 1 MLTCNYCDKRGLLIYPVRYAVASPYGAQAVPALSGNFRIEGAPQQIGEARYTLRALRPGY 60 Query: 61 LYTYDEKRGRLKAYVVMPTGHLWNYPLEYKPPHPAKIRFACVDTGELVRAYCVDIIHTAA 120 LYTYDE+R RLKAYVVMPTGHLWN+PLEY PPHP +I F+C+D GE+VRAYCVDI HT + Sbjct: 61 LYTYDERRARLKAYVVMPTGHLWNFPLEYTPPHPGRIPFSCIDPGEIVRAYCVDIAHTPS 120 Query: 121 DPAGNFWIGWSNVQWTKALLKKVSDAGWRKKHMQCIDIAAMLAGSAAHTGEFNASMDKVA 180 DPA FWIGWSN WTKAL KKVS+ WRKKHMQCID+AAMLAG+A HTGEFNAS VA Sbjct: 121 DPATRFWIGWSNTTWTKALQKKVSETNWRKKHMQCIDVAAMLAGTAPHTGEFNASKQHVA 180 Query: 181 HFAADNTALQKAFSFSNTPSRSEPSKQAAAPHFTAIMAKHEPHHQGFIAAINDPVGMAND 240 HFAAD + + KAFSFSNTP E + A A IM PHH+G++ A+NDPVG+AND Sbjct: 181 HFAADVSEMAKAFSFSNTPPAEENKRHAWAGKMADIMTAQAPHHKGYVVAVNDPVGVAND 240 Query: 241 LSELTMPDLLAGFDEKLHQRKMIADLLALTEHHVRQEARQEVVFSDAVAEASAHHPDGDV 300 LSEL +P L AGFDEKL+Q K IADLL +TE VRQEAR + F VA+ S ++PDGD Sbjct: 241 LSELCVPGLAAGFDEKLYQAKTIADLLTMTEKGVRQEARNAIAFDAKVAQMSENYPDGDT 300 Query: 301 YNGLKTLGAMFKLGGINRHEKKLQEQRKKYGEDLAGRQQAAADDAWHDLTHDNGKPTLDE 360 Y LKT+ + K GG R+ +K E+RKKYG D A RQQAA D+AWH+LTHD+GKPTLD+ Sbjct: 301 YGSLKTVWGVIKAGGPARYVRKQSEKRKKYGADQAARQQAAEDEAWHELTHDDGKPTLDQ 360 Query: 361 AALKAFPAIYDAALKQFEPQLLQLLMAHVGWLKSEQLANWMEGVHDDADIRSGYAYSESM 420 LKAFPAIYDAALK FEP+ L+L+ AHV WLKSEQLANWMEGVHD DIRSG+AYSES+ Sbjct: 361 QKLKAFPAIYDAALKAFEPRFLKLVTAHVAWLKSEQLANWMEGVHDVNDIRSGHAYSESL 420 Query: 421 SQCIGKAACSKPCIEQLMRWISSDNLKDTRNLYGRALLLNQADIIAATEVQLKGSDIQIE 480 +QCIGKA S+ C +QL W+S L D NL+GRALL NQ D+IAATE QLKGSDIQ E Sbjct: 421 AQCIGKAVASQACQDQLALWLSRSRLSDPGNLFGRALLYNQEDLIAATESQLKGSDIQFE 480 Query: 481 NILNIYKGAIERLHNAGHAEQLIDRLALTTGNVIAKAISESGSLLAKGLAQIHLQLMAGV 540 NILN+YKGA+ RL +A QLIDRLALTT NVI K++S++ +A+ LA IHL L+AGV Sbjct: 481 NILNLYKGAVARLDVTPYAPQLIDRLALTTANVIGKSLSDASQSVARSLAMIHLHLLAGV 540 Query: 541 AIKVSNMSSADVARWAIAEAKEKGIKLETNRQKTRADARTEAGRAIKRAKVEKHVV-YEL 599 IK SNMS+ADV+RWAI +AK +GI+L+T+RQ+TR DA +A A++ + + + ++ YEL Sbjct: 541 TIKASNMSAADVSRWAIEQAKAQGIELKTDRQQTRRDAYHQAKGAVRSSAMGRRMIAYEL 600 Query: 600 DIARLEKEGRIAPGSIKGIGLPGFAMTQKWLGSGAPRDFKLGVVTMIVQMVALNFAMKDL 659 DIA LE+EGRIA GSIKGI +P A+ QKWLGS P +FKLGV T +VQM+ALNFAM DL Sbjct: 601 DIASLEREGRIAAGSIKGISIPDIALAQKWLGSSVPAEFKLGVATTLVQMLALNFAMDDL 660 Query: 660 VGNDQFNQVETRVKTTLAIISLSATIVETAAASLQKSVEHPLAVFIRGQWAIEKKWMSTL 719 NDQFN+ ET+VK +A +SL+AT+VET A S+QKSV HP FI GQWAI+KK + + Sbjct: 661 AHNDQFNERETQVKAAVAAVSLTATLVETVAVSVQKSVGHPSGAFIYGQWAIDKK-LGKI 719 Query: 720 LKGARMAGAGAGILAGLFDLAFNAFPAYRDENYLLMTLYGLNGLSGIGIAFAAYYSIAAV 779 ++GAR+AG GAGILAG FD+A +A A+ ++ LL LY NG GI+ AAY +I + Sbjct: 720 IQGARLAGMGAGILAGAFDIAVSARVAFGEKKQLLGWLYLANGTLSAGISIAAYSTIGGI 779 Query: 780 WTPLLIVSFAISVSIAMVNNSQLKTWISRCDFSSKEKYSSFEEQINAF 827 + P L+ SF +S++IA+VN+S LKTWISRC+F EKY SFE Q+ A+ Sbjct: 780 FWPFLVSSFLVSIAIALVNDSALKTWISRCEFGLGEKYGSFEGQLKAY 827 >gi|300310085|ref|YP_003774177.1| hypothetical protein Hsero_0750 [Herbaspirillum seropedicae SmR1] Length = 1070 Score = 63.9 bits (154), Expect = 5e-11, Method: Compositional matrix adjust. Identities = 99/412 (24%), Positives = 155/412 (37%), Gaps = 65/412 (15%) Query: 2 NG-CNFCDKKGLLIYPVRYAVASPYGAAGVPGLSGHFKIEG-APQAVGAAKYTLRALRAG 59 NG C+FC + G I P+RYAV Y A G +++ A Q + +YTLR LR G Sbjct: 32 NGQCSFCQRTGYPILPLRYAVKPSYVTAIGAGPQSLPQMDKFAAQPLKGNRYTLRVLRKG 91 Query: 60 YLYTYDEKRGRLKAYVVMPTGHLWNYPLEYKPPHP-----AKIRFACVDTGELVRAYCVD 114 Y++ Y +G + Y V G+L L P P + AC G + A ++ Sbjct: 92 YVHVYMGVKGHWQTYAVTDDGYL---RLLKNPDDPDAKGNRPLTEACKRDGHNIPASFIN 148 Query: 115 IIHTAADPAGNFWIGWSNVQWTKAL---LKKVSDAGWRKKHMQCIDIAAMLA-------- 163 I + WIG+S + W K + + DA MQ +D+ M Sbjct: 149 I----PEKYKKVWIGFSELPWRKEVRSAFEAKPDA-----RMQQVDLTQMANAPAQVPNA 199 Query: 164 ------GSAAH---------TGEFNASMDKVAHFAADNTALQKAFSFSNTPSRSEPSKQA 208 G++ H G++ + A + + F + + S Q Sbjct: 200 LEITPDGTSLHAYVEEYARDAGDYAKRLQYPAPVVSAEKVSKGDFQWESLHGNYPRSGQL 259 Query: 209 AA--PHFTAIMAKHEPHHQGFIAAINDPVGMANDLSELTMPDLLAGFDEKLHQRKMIADL 266 AA H TA K + + ++DPVGM +L+ + A D M + Sbjct: 260 AAVGEHATAYQKKTKRKMAALV--LHDPVGMVQELNGAVSRVVKARQD--FGAAVMRPLI 315 Query: 267 LALTEHHVRQEARQEVVFSDAVAEASAHHPDGDVYNGLKTLGAMFKLGGINRHEKKLQEQ 326 ++ + +++ Q + E PD +++ M GI+ + K + Sbjct: 316 VSQSILGLKKFFEQAASANREAQEKKDKKPDKQYELDTESI-PMSGTAGISVYNMKTTTR 374 Query: 327 RKKYGEDLAGRQQAAADDAWHDLTHDNGKPTLDEAALKAFPAIYDAALKQFE 378 A R Q ADDAW + DE A AF Y A+K FE Sbjct: 375 --------AERAQRDADDAWIRIAR-----RYDEGARAAFDQRYTEAMKGFE 413 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.133 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,502,029 Number of extensions: 103129 Number of successful extensions: 325 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 321 Number of HSP's successfully gapped: 4 Length of query: 833 Length of database: 1,619,870 Length adjustment: 93 Effective length of query: 740 Effective length of database: 1,179,515 Effective search space: 872841100 Effective search space used: 872841100 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 135 (56.6 bits)