BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3360.p (493 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312696|ref|YP_003776788.1| D-xylulose kinase [Herbaspirill... 878 0.0 gi|300309787|ref|YP_003773879.1| sugar (pentulose and hexulose) ... 482 e-137 gi|300310425|ref|YP_003774517.1| gluconokinase [Herbaspirillum s... 120 2e-28 gi|300310306|ref|YP_003774398.1| glycerol kinase [Herbaspirillum... 106 5e-24 gi|300310399|ref|YP_003774491.1| D-ribulose/ribitol kinase [Herb... 66 6e-12 >gi|300312696|ref|YP_003776788.1| D-xylulose kinase [Herbaspirillum seropedicae SmR1] Length = 493 Score = 878 bits (2268), Expect = 0.0, Method: Compositional matrix adjust. Identities = 437/491 (89%), Positives = 453/491 (92%) Query: 1 LSYLGIDLGTSEVKLVLTDPDSNIIATRSARLRVDHPHPLWSEQAPQAWWNATLDAIATL 60 +SYLGIDLGTSEVKLVLTD DSN+IA+ SARLRVD+PHPLWSEQAPQ+WWNATLDAIA L Sbjct: 1 MSYLGIDLGTSEVKLVLTDEDSNVIASTSARLRVDNPHPLWSEQAPQSWWNATLDAIAAL 60 Query: 61 RATAPQAFQALRGIGISGQMHGATLLDRNGNVLRPAILWNDMRAHAECVELEALVPDAAD 120 R APQAFQALRGIG+SGQMHGATLLDRNGNVLRPAILWNDMRAHAECVELEALVPDA Sbjct: 61 RTQAPQAFQALRGIGLSGQMHGATLLDRNGNVLRPAILWNDMRAHAECVELEALVPDAVA 120 Query: 121 ITGNRAMPGFTAPKLLWLSKYEPAVYRAIDKVLLPKDYLGWKLTGEFVSEMSDAAGTLWL 180 ITGNRAMPGFTAPKLLWLSKYEPAVYRAIDKVLLPKD+LGWKLTGEFVSEMSDAAGTLWL Sbjct: 121 ITGNRAMPGFTAPKLLWLSKYEPAVYRAIDKVLLPKDFLGWKLTGEFVSEMSDAAGTLWL 180 Query: 181 DVARRDWSERMLHATGLSRSHMPRLVEGSAVAGHLREDLRREWGISAPVVVAGGAGDNAA 240 DVARRDWSERML ATGL RSHMPRLVEGSAVAG LR++LRREWGIS PVVVAGGAGDNAA Sbjct: 181 DVARRDWSERMLGATGLDRSHMPRLVEGSAVAGQLRDELRREWGISGPVVVAGGAGDNAA 240 Query: 241 SAVGIGVIQAGDAFLSLGSSGVLFAATAQHAPNPQQGVHAFCHCLPGQWHQMSVILSAAA 300 SAVGIGVI+AGDAFLSLGSSGVLFAATA HAPN QQGVHAFCHCLPGQWHQMSVILSAAA Sbjct: 241 SAVGIGVIRAGDAFLSLGSSGVLFAATAHHAPNAQQGVHAFCHCLPGQWHQMSVILSAAA 300 Query: 301 SLHWLAGVLGREVPELVSAAERLTPEQQAQAPLFLPYLNGERTPHNDAAAKGVLFGMTSA 360 SLHWL+GV R V ELVS AERL+ QQAQAPLFLPYLNGERTPHNDAAAKGVLFGMT A Sbjct: 301 SLHWLSGVTARPVAELVSGAERLSAGQQAQAPLFLPYLNGERTPHNDAAAKGVLFGMTPA 360 Query: 361 HEAAHLAYAVMEGVAFAMADGYAALQAAGTVLHSAAFVGGGSRSAFWGQLCATALGIPLR 420 HE+AHLAYAVMEGVAFAMADGYAALQAAGT L SAAFVGGGSRSAFWG LCATALGIPLR Sbjct: 361 HESAHLAYAVMEGVAFAMADGYAALQAAGTTLESAAFVGGGSRSAFWGVLCATALGIPLR 420 Query: 421 HHAGAEVGAALGAARLGRLAQTGEDVRDVCVAPEVLERYAPDQARQSQLMRRLEQYRRLY 480 HAGAEVGAALGAARLGRLA+TGED VC+ PEVLE Y PD ARQ+QL RRL QYRRLY Sbjct: 421 RHAGAEVGAALGAARLGRLARTGEDAASVCIPPEVLEWYRPDAARQAQLQRRLAQYRRLY 480 Query: 481 PCLAPEFFAFS 491 LA EF AFS Sbjct: 481 RSLADEFIAFS 491 >gi|300309787|ref|YP_003773879.1| sugar (pentulose and hexulose) kinase [Herbaspirillum seropedicae SmR1] Length = 490 Score = 482 bits (1241), Expect = e-137, Method: Compositional matrix adjust. Identities = 261/484 (53%), Positives = 330/484 (68%), Gaps = 2/484 (0%) Query: 3 YLGIDLGTSEVKLVLTDPDSNIIATRSARLRVDHPHPLWSEQAPQAWWNATLDAIATLRA 62 YLGIDLGTSEVK V+ D +++A + L V PHP WSEQAP WW AT D +A LR Sbjct: 2 YLGIDLGTSEVKAVVIDAQGSLVALAGSTLNVARPHPRWSEQAPADWWQATCDTVAKLRT 61 Query: 63 T-APQAFQALRGIGISGQMHGATLLDRNGNVLRPAILWNDMRAHAECVELEALVPDAADI 121 + F ++R IG+SGQMHGA LLD VLRPAILW+D R+ EC ELE+ P I Sbjct: 62 QLGSERFGSIRAIGLSGQMHGAVLLDERDEVLRPAILWSDSRSAPECAELESRAPRLHGI 121 Query: 122 TGNRAMPGFTAPKLLWLSKYEPAVYRAIDKVLLPKDYLGWKLTGEFVSEMSDAAGTLWLD 181 GN AMPGFTAPKLLW++++EP ++ I VLLPKD+L K+TG VS+ SDAAGTLWLD Sbjct: 122 AGNLAMPGFTAPKLLWVARHEPQLFARIATVLLPKDWLRLKMTGRKVSDPSDAAGTLWLD 181 Query: 182 VARRDWSERMLHATGLSRSHMPRLVEGSAVAGHLREDLRREWGISAPVVVAGGAGDNAAS 241 V R+WS+ +L A+G+ R MP LV+GSAV+G L ++ + WG+ + V+VAGGAGD AAS Sbjct: 182 VEGRNWSDELLAASGMRRDQMPALVDGSAVSGSLLPEVAQAWGLRSDVIVAGGAGDGAAS 241 Query: 242 AVGIGVIQAGDAFLSLGSSGVLFAATAQHAPNPQQGVHAFCHCLPGQWHQMSVILSAAAS 301 AVGIG ++ GD FLSLG+SGVLF + PNP + +HAFCH LP +WHQMSV+LSAA+ Sbjct: 242 AVGIGAVKPGDGFLSLGTSGVLFVVNDRFRPNPGRAIHAFCHTLPQRWHQMSVMLSAASC 301 Query: 302 LHWLAGVLGREVPELVSAAERLTPEQQAQAPLFLPYLNGERTPHNDAAAKGVLFGMTSAH 361 L W + + L++ E+L + APLFLPYL+GERTPHND A GV G+T H Sbjct: 302 LRWFCRLCSVDEKSLLAEIEQLDEQACNNAPLFLPYLSGERTPHNDPYATGVFHGLTPEH 361 Query: 362 EAAHLAYAVMEGVAFAMADGYAALQAAGTVLHSAAFVGGGSRSAFWGQLCATALGIPLRH 421 + A L YAV+EGVAF M DG AL+AAGT + + VGGG+RSA+W QL A AL + + Sbjct: 362 QRAALGYAVLEGVAFGMVDGLDALRAAGTDVAELSLVGGGARSAYWAQLLADALQVRIVT 421 Query: 422 HAGAEVGAALGAARLGRLAQTGEDVRDVCVAPEVLERYAPDQARQSQLMRRLEQYRRLYP 481 H G+E G ALGAARL LA G++ +VC P Y PD AR + L RL++YR +Y Sbjct: 422 HVGSEAGGALGAARLAWLADGGDEA-EVCRKPAQQALYQPDPARHAALQARLQRYRAIYA 480 Query: 482 CLAP 485 AP Sbjct: 481 PFAP 484 >gi|300310425|ref|YP_003774517.1| gluconokinase [Herbaspirillum seropedicae SmR1] Length = 520 Score = 120 bits (302), Expect = 2e-28, Method: Compositional matrix adjust. Identities = 120/451 (26%), Positives = 192/451 (42%), Gaps = 24/451 (5%) Query: 4 LGIDLGTSEVKLVLTDPDSNIIATRSARLRVDHPHPLWSEQAPQAWWNATLDAIATLRAT 63 LG+D+GT+ K V+ D ++A + V P +EQ P+ A L +IA Sbjct: 11 LGVDIGTTSTKSVVFTLDGKVVAQHAEEYPVLCTEPGMAEQDPEQIVAAALASIAGAVKA 70 Query: 64 APQAFQALRGIGISGQMHGATLLDRNGNVLRPAILWNDMRAH--AECVELEALVPDAADI 121 A A Q + + S MH LD +L +I W D+RA AE ++ E Sbjct: 71 AKAAPQEIALLSFSAAMHSVIALDAENRLLSNSITWGDIRASVWAERIKHEHDGNAIYRR 130 Query: 122 TGNRAMPGFTAPKLLWLSKYEPAVYRAIDKVLLPKDYLGWKLTGEFVSEMSDAAGTLWLD 181 TG P KL+W+ +P V+ + + K+YL ++L G++V + S A+ T + Sbjct: 131 TGTPVHPMSPLCKLMWMRHEKPDVFHRAARFVGIKEYLLYQLFGQWVVDHSIASATGMFN 190 Query: 182 VARRDWSERMLHATGLSRSHMPRLVEGSAVAGHLREDLRREWGISAPVVVAGGAGDNAAS 241 + W + L G+ +P V + L ++ + G+S GA D S Sbjct: 191 LRELAWDQGALALLGVRPDQLPTPVPTTHRLPALSSEMAQRLGLSVDTPFIIGANDGVLS 250 Query: 242 AVGIGVIQAGDAFLSLGSSGVLFAATAQHAPNPQQGVHAFCHCLPGQ-WHQMSVILSAAA 300 +G+ I+ G +++G+SG + + +PQ + FC+ L + W + + Sbjct: 251 NLGVNAIEIGHVAVTIGTSGAMRTVIDEPRTDPQGRL--FCYALTEKHWVVGGPVNNGGN 308 Query: 301 SLHWL--------------AGVLGREVPELVSAAERLTPEQQAQAPLFLPYLNGERTPHN 346 W+ GV E L AE++ P A+ LF P++ GER P Sbjct: 309 IFRWVRDELATAEAAAAREEGVDPYEA--LTRIAEKVRP--GAEGLLFHPFMAGERAPLW 364 Query: 347 DAAAKGVLFGMTSAHEAAHLAYAVMEGVAFAMADGYAALQAAGTVLHSAAFVGGGSRSAF 406 +A +G FG+ H H+ A +EGV F + AL+ GG +RSA Sbjct: 365 NADLRGSFFGLALHHGKHHMIRAALEGVIFNLYSILPALEELVGPTKRMMATGGFARSAL 424 Query: 407 WGQLCATALGIPLRHHAGAEVGAALGAARLG 437 W Q+ A + E + LGAA LG Sbjct: 425 WRQMMADIFNREVVVPESVE-SSCLGAAVLG 454 >gi|300310306|ref|YP_003774398.1| glycerol kinase [Herbaspirillum seropedicae SmR1] Length = 495 Score = 106 bits (264), Expect = 5e-24, Method: Compositional matrix adjust. Identities = 116/451 (25%), Positives = 194/451 (43%), Gaps = 22/451 (4%) Query: 4 LGIDLGTSEVKLVLTDPDSNIIATRSARLRVDHPHPLWSEQAPQAWWNATLDAIATLRAT 63 L +D GTS + ++ D I+AT P P W E P W++ L + A Sbjct: 5 LALDQGTSSSRSIIFDQSGAIVATAQQEFPQIFPQPGWVEHDPMDLWHSQLQTAQKVLAQ 64 Query: 64 APQAFQALRGIGISGQMHGATLLDR-NGNVLRPAILWNDMRAHAECVEL--EALVPDAAD 120 + L +GI+ Q + +R G + A++W D RA C L L Sbjct: 65 SGIKASELTALGITNQRETTVVWERATGKPVYNAVVWQDRRAEVVCAALRERGLGEAIQK 124 Query: 121 ITGNRAMPGFTAPKLLWLSKYEPAVY-RAIDKVLL---PKDYLGWKLTGE--FVSEMSDA 174 TG P F+ KL W+ + + RA LL +L W LTG VS++S+A Sbjct: 125 KTGLVLDPYFSGTKLRWILDHVDGLRARAERGELLFGTVDSWLVWNLTGGRLHVSDVSNA 184 Query: 175 AGTLWLDVARRDWSERMLHATGLSRSHMPRLVEGSAVAGHLREDLRREWGISAPVVVAGG 234 + T+ ++ W E +L G+ + +P+ V+ SA HL ++ + + A V + G Sbjct: 185 SRTMLWNIHTGAWDEELLQWLGIPAAMLPQ-VQPSA---HLYGEIDAQ-HLGAAVKIGGI 239 Query: 235 AGDNAASAVGIGVIQAGDAFLSLGSSGVLFAATAQHAPNPQQGVHAFCHCLPGQWHQMSV 294 AGD ++ G ++ G A + G+ + T G+ + C + Q ++ Sbjct: 240 AGDQQSALFGQACVRPGMAKNTYGTGCFMLLHTGDQCATSHNGLISTAACQVDEQKQYAL 299 Query: 295 ---ILSAAASLHWLAGVLGREVPELVSAAERLTPE-QQAQAPLFLPYLNGERTPHNDAAA 350 + A + WL G + + + E L + + +F+P G P+ + +A Sbjct: 300 EGSVFIGGAVVQWLRD--GLKAIKTSTDVEGLACSVEDSGGVVFVPSFTGLGAPYWNPSA 357 Query: 351 KGVLFGMTSAHEAAHLAYAVMEGVAF-AMADGYAALQAAGTVLHSAAFVGGGSRSAFWGQ 409 KG + G++ AH+A A +E +AF + A A ++ A + + GG + + Q Sbjct: 358 KGAIVGLSRGSTVAHIARAALESIAFQSTALLNAMVRDAVSPITELRVDGGAAANNLLLQ 417 Query: 410 LCATALGIPLRHHAGAEVGAALGAARLGRLA 440 A LGIP+ E ALGAA L +A Sbjct: 418 FQADLLGIPVIRPQVTET-TALGAAYLAGIA 447 >gi|300310399|ref|YP_003774491.1| D-ribulose/ribitol kinase [Herbaspirillum seropedicae SmR1] Length = 548 Score = 66.2 bits (160), Expect = 6e-12, Method: Compositional matrix adjust. Identities = 120/485 (24%), Positives = 178/485 (36%), Gaps = 55/485 (11%) Query: 4 LGIDLGTSEVKLVLTDPDSNIIATRSARLRVD---HPHPLWSEQAPQAWWNATLDAIATL 60 +GID+GT + + DP ++A SAR +D EQ+ W A A+ Sbjct: 6 IGIDVGTGSARAGVFDPQGQLLA--SARHDIDLFRDERRARVEQSSAQIWEAVCQAVRAA 63 Query: 61 RATAPQAFQALRGIGISGQ----MHGATL-LDRNGNVLRPAILWNDMRAHAECVELEALV 115 + A+ GIG+ + GA+ + G+ R I+W D RA + + + Sbjct: 64 VTKSGIDPAAVAGIGVDATCSLVVQGASAGVGDPGHPERDVIVWMDHRALEQAQRINRMG 123 Query: 116 PDAADITGNRAMPGFTAPKLLWLSKYEPAVYRAIDKVLLPKDYLGWKLTGEFVSEMSDAA 175 G P PKLLWL + P VY + D+L WK TG S Sbjct: 124 HAVLSYVGGVISPEMETPKLLWLKENLPEVYASAAHFFDLTDFLTWKATGSL-QRSSCTV 182 Query: 176 GTLWLDVARR-DWSERMLHATGL--------SRSHMPRLVEGSAVAGHLREDLRREWGIS 226 W +A W GL +R + G+ +AG L + + G+ Sbjct: 183 TCKWTYLAHEAGWDASYFRQIGLGDLAEAGFARIGAEVVWPGTPLAGGLTQTAAQAMGLR 242 Query: 227 APVVVAGGAGDNAASAVGIGVIQA----GDAFLSL----GSSGVLFAATAQHAPNPQQGV 278 + VA G D A A G+G + A GDA + G+S + A P Sbjct: 243 PGIAVAAGLID--AHAGGVGTVAARGGQGDAAACMAYVFGTSSCTMTSHADPVLVPGVWG 300 Query: 279 HAFCHCLPGQWHQMSVILSAAASLHWLAGVLGREVPELVSAAERLTPE------QQAQAP 332 + PG W +A A++ L L P+ A R E QQA A Sbjct: 301 PYYKAMAPGMWLNEGGQSAAGAAIDHLL-RLHPATPQAREEAARAQMELPQWLAQQALAA 359 Query: 333 L--------------FLPYLNGERTPHNDAAAKGVLFGMTSAHEAAHLAYAVMEGVA--- 375 + +P G R+P D A+ L G+ H+ L + G+ Sbjct: 360 VDHPSEAVWLAGQLTVVPEFLGNRSPLADPQARAALVGLGMEHDIDSLVALYVAGLCSLG 419 Query: 376 FAMADGYAALQAAGTVLHSAAFVGGGSRSAFWGQLCATALGIPLRHHAGAEVGAALGAAR 435 + + A A G + S + GG QL A A G+P+ A E LG+A Sbjct: 420 YGLRQIIEAQAACGVRVQSISVSGGAGVHPLTRQLLADATGMPVEITASPEP-VLLGSAM 478 Query: 436 LGRLA 440 L +A Sbjct: 479 LAAVA 483 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.133 0.412 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,617,273 Number of extensions: 68908 Number of successful extensions: 256 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 248 Number of HSP's successfully gapped: 5 Length of query: 493 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 404 Effective length of database: 1,198,455 Effective search space: 484175820 Effective search space used: 484175820 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)