BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3357.p (345 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312693|ref|YP_003776785.1| D-xylulose reductase [Herbaspir... 629 0.0 gi|300310427|ref|YP_003774519.1| L-idonate 5-dehydrogenase [Herb... 164 8e-42 gi|300312106|ref|YP_003776198.1| zinc-type alcohol dehydrogenase... 97 2e-21 gi|300310297|ref|YP_003774389.1| alcohol dehydrogenase [Herbaspi... 80 3e-16 gi|300309893|ref|YP_003773985.1| glutathione-dependent formaldeh... 72 6e-14 >gi|300312693|ref|YP_003776785.1| D-xylulose reductase [Herbaspirillum seropedicae SmR1] Length = 345 Score = 629 bits (1621), Expect = 0.0, Method: Compositional matrix adjust. Identities = 316/345 (91%), Positives = 332/345 (96%) Query: 1 MQALVLEATRQLSLREIDLPQQVGPQDVRIRIHTVGICGSDLHYYTHGSIGPFKVEAPMV 60 MQALVLEATR+L LREIDLPQQ+G QDVRIRIHTVGICGSDLHYYTHGSIGPFKVEAPMV Sbjct: 1 MQALVLEATRELKLREIDLPQQMGAQDVRIRIHTVGICGSDLHYYTHGSIGPFKVEAPMV 60 Query: 61 LGHEASGTVIEVGSAVRHLKVGDRVCMEPGIPRLDSPATLRGMYNLDPSVRFWATPPIHG 120 LGHEASGTVIEVGSAV HLKVGDRVCMEPGIPRLDSPATLRGMYNLDP+VRFWATPPIHG Sbjct: 61 LGHEASGTVIEVGSAVSHLKVGDRVCMEPGIPRLDSPATLRGMYNLDPAVRFWATPPIHG 120 Query: 121 CLTDSVVHPAAFTYRLPDNVSFAEGAIVEPLSIGLQAATKARMKPGDTAVVIGAGTIGAM 180 CLT SVVHPAAFTYRLPDNVSFAEGAIVEPLSIGLQAATKARMKPGDTAVVIGAGTIGAM Sbjct: 121 CLTGSVVHPAAFTYRLPDNVSFAEGAIVEPLSIGLQAATKARMKPGDTAVVIGAGTIGAM 180 Query: 181 TALAALAGGAARVILADVVAQKLAHFAGNPAVTTVDVSQQSLVEVVQQRTEGWGADVVFE 240 TALAALAGGAARVILADVVA+KLAHFA NPAV TVDV++++L +VV+Q T+GWGADVVFE Sbjct: 181 TALAALAGGAARVILADVVAEKLAHFADNPAVITVDVTRETLTDVVRQATDGWGADVVFE 240 Query: 241 ASGHAGVYHHLLEMICPGGCAVLVGMPAAPVALDVVAMQTREVRLESVFRYANIFPRALA 300 ASGHAGVY LL+++CPGGCAVLVGMP APVALDVVAMQT+EVRLESVFRYANIFPRALA Sbjct: 241 ASGHAGVYQTLLDLVCPGGCAVLVGMPPAPVALDVVAMQTKEVRLESVFRYANIFPRALA 300 Query: 301 LISSGMIDVKPFISRSFPFSQSIRAFEEAASGQPQDVKIQIEMAG 345 LISSGMIDVKPFISR FPFSQSIRAFEEAASG+PQDVKIQIEM G Sbjct: 301 LISSGMIDVKPFISRKFPFSQSIRAFEEAASGRPQDVKIQIEMEG 345 >gi|300310427|ref|YP_003774519.1| L-idonate 5-dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 345 Score = 164 bits (416), Expect = 8e-42, Method: Compositional matrix adjust. Identities = 108/344 (31%), Positives = 175/344 (50%), Gaps = 8/344 (2%) Query: 2 QALVLEATRQLSLREIDLPQQVGPQDVRIRIHTVGICGSDLHYYTHGSIGPFKVEAPMVL 61 Q LV+ A L ++ +D + PQ +++++ GICGSDLHYY HG G +++ PMVL Sbjct: 3 QGLVIHAPHDLRIQALD-TGALQPQQLKVKVKAGGICGSDLHYYHHGGFGVVRIKEPMVL 61 Query: 62 GHEASGTVIEVGSAVRHLKVGDRVCMEPGIPRLDSPATLRGMYNLDPSVRFWA----TPP 117 GHE SG + EVG+AV + G R+ + P P G N +RF+ TP Sbjct: 62 GHEVSGQIAEVGTAVTDIAPGTRIAISPSRPCGACKYCQEGKQNHCLDMRFYGSAMRTPH 121 Query: 118 IHGCLTDSVVHPAAFTYRLPDNVSFAEGAIVEPLSIGLQAATKARMKPGDTAVVIGAGTI 177 + G ++ + + + D+VS E A+ EPLS+ L A +A G +V G G I Sbjct: 122 VQGAFRQEIIIERSQAHVVADHVSDGEAAMAEPLSVALHAVRRAGPLVGKRVLVTGCGPI 181 Query: 178 GAMTALAALAGGAARVILADVVAQKLAHFAGNPAVTTVDVSQQSLVEVVQQRTEGWGADV 237 GA+ +AA GA V++ DV + L A + +++++ +G DV Sbjct: 182 GALVVVAARRAGALEVVVTDVASMPLQAALQVGADSAINMAETPAALKAYAADKGT-FDV 240 Query: 238 VFEASGHAGVYHHLLEMICPGGCAVLVGMPAAPVALDVVAMQTREVRLESVFRYANIFPR 297 +FEASG+ LE + P V VG+ + + +E+ FR+ F Sbjct: 241 LFEASGNERALVGALEALRPQAIIVQVGL-GGDIQFPANLLVGKELEWRGAFRFHEEFAM 299 Query: 298 ALALISSGMIDVKPFISRSFPFSQSIRAFEEAASGQPQDVKIQI 341 A+ L++ ++DVKP I+ ++P +Q++ AF E A + + +K+QI Sbjct: 300 AVELMNRKLVDVKPLITATYPIAQAVEAF-EIAGDRSRMMKVQI 342 >gi|300312106|ref|YP_003776198.1| zinc-type alcohol dehydrogenase transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 374 Score = 96.7 bits (239), Expect = 2e-21, Method: Compositional matrix adjust. Identities = 107/357 (29%), Positives = 144/357 (40%), Gaps = 42/357 (11%) Query: 9 TRQLSLREIDLPQQVGPQDVRIRIHTVGICGSDLHYYTHGSIGPFKVEAPMVLGHEASGT 68 ++ L + E+DL GP +V +R+ G+C SDL T + P V PM LGHEAS Sbjct: 20 SQPLKIEELDL-DPPGPGEVLVRMKAAGLCHSDLSVIT--GVRPRPV--PMALGHEASAE 74 Query: 69 VIEVGSAVRHLKVGDRV-------------CMEPGIPRLDSPATLRGMYN--LDPSVRFW 113 V+EVGS V L+ GD V CME G P L P L R Sbjct: 75 VVEVGSGVNDLRAGDLVVLVFVPSCGHCLPCME-GRPALCEPGAATNTVGELLSGQRRLR 133 Query: 114 -ATPPIHGCLTDSVVHPAAFTYRLPDNVSFAEGAIVEPLSIGL----------QAATKAR 162 T P+H L S A R A ++P+ L A A Sbjct: 134 DKTQPVHHHLGVSAFADHAVVSR---RSCVKIEADIDPVQAALFGCAVLTGVGAAVNTAE 190 Query: 163 MKPGDTAVVIGAGTIGAMTALAALAGGAARVILADVVAQKLAHFAGNPAVTTVDVSQQSL 222 +K G TA V+G G +G L ALA GA VI D+ KLA A TV+ Sbjct: 191 VKAGTTAAVLGLGGVGLCAMLGALASGAREVIAVDLHDSKLALARSLGATATVNARDPDA 250 Query: 223 VEVVQQRTEGWGADVVFEASGHAGVYHHLLEMICPGGCAVLVGMPA--APVALDVVAMQT 280 VE V+ T G G D FE +G M GG V G+PA AL V++ Sbjct: 251 VEKVRALTRG-GVDYAFEMAGSVQAMEAAYRMTRRGGMTVTAGLPAPGQHWALQQVSLVA 309 Query: 281 REVRLESVFRYANI----FPRALALISSGMIDVKPFISRSFPFSQSIRAFEEAASGQ 333 E ++ + + + PR + L +G + V + + R F+ G+ Sbjct: 310 EERTVKGSYIGSCVPVRDIPRYIGLFQAGKLPVDRLMGERLALADINRGFDRLHGGE 366 >gi|300310297|ref|YP_003774389.1| alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 341 Score = 79.7 bits (195), Expect = 3e-16, Method: Compositional matrix adjust. Identities = 81/283 (28%), Positives = 120/283 (42%), Gaps = 25/283 (8%) Query: 3 ALVLEATRQLSLREIDLPQQVGPQDVRIRIHTVGICGSDLHYYTHGSIGPFKVEAPMVLG 62 A+V E + LS+ ++ +P P + ++ G+C +DLH HG P K P + G Sbjct: 8 AVVREFGKPLSIEQVPVPTPA-PGQILVKFEASGVCHTDLHA-AHGDW-PVKPTPPFIPG 64 Query: 63 HEASGTVIEVGSAVRHLKVGDRVCMEPGIPRLDSPATLRGMYNLDPSVRFWAT------- 115 HE +G V VG+ V+H+K GDRV G+P L + P W T Sbjct: 65 HEGTGYVAAVGAGVKHVKEGDRV----GVPWLHTACGC-----CSPCRTGWETLCAEQQN 115 Query: 116 --PPIHGCLTDSVVHPAAFTYRLPDNVSFAEGAIVEPLSIGLQAATK-ARMKPGDTAVVI 172 ++G + + F LPDN+ F A V + + K ++PG+ V+ Sbjct: 116 TGYSVNGSFAEYGLADPKFVGHLPDNLDFGPAAPVLCAGVTVYKGLKETEVRPGEWVVIS 175 Query: 173 GAGTIGAMTALAALAGGAARVILADVVAQKLAHFAGNPAVTTVDVSQQSLVEVVQQRTEG 232 G G +G M A A G V+ AD+ KLA A TVD + V VQ+ G Sbjct: 176 GIGGLGHMAVQYAKAMG-MHVVAADIHEDKLALAKKLGADLTVDGRNHNAVAEVQRIIGG 234 Query: 233 WGADVVFEASGHAGVYHHLLEMICPGGCAVLVGMPAAPVALDV 275 +V S A + G LVG+P ++L V Sbjct: 235 AHGALVTAVSPKA--MEQAFGFLRARGTMALVGLPPGDISLPV 275 >gi|300309893|ref|YP_003773985.1| glutathione-dependent formaldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 386 Score = 72.0 bits (175), Expect = 6e-14, Method: Compositional matrix adjust. Identities = 73/267 (27%), Positives = 111/267 (41%), Gaps = 27/267 (10%) Query: 1 MQALVLEATRQLSLREIDLPQQVGPQDVRIRIHTVGICGSDLHYYTHGSIGPFKVEAPMV 60 M+AL+ + + + + P DV +++ ICGSDLH Y G I K A + Sbjct: 1 MRALLYHSAHDVRVETVPDPIIQEADDVILKVTATAICGSDLHLY-RGKIPATK--AGDI 57 Query: 61 LGHEASGTVIEVGSAVRHLKVGDRV-------------CMEPGIPRLDSPATLRGMYNLD 107 LGHE G V+E G A++ LK GDRV C + ++ RG Sbjct: 58 LGHEFMGVVVEAGPAIKRLKKGDRVVVPFVIACGQCFFCDQHLYAACETTNPDRGALMNA 117 Query: 108 PSVRFWATPPIHGCLTDSVVHPAAFTYRLPD--------NVSFAEGAIV---EPLSIGLQ 156 +R A + L + A R+P + + A+ ++ + L G Q Sbjct: 118 KGIRSGAAMFGYSHLYGGMPGGQAEYVRVPKADVGPIRIDSALADEQVLFLSDILPTGYQ 177 Query: 157 AATKARMKPGDTAVVIGAGTIGAMTALAALAGGAARVILADVVAQKLAHFAGNPAVTTVD 216 A A ++ G + + GAG +G M A +A GA R+ + D A +L V V+ Sbjct: 178 AVKNAGVREGSSVAIYGAGPVGQMAAASARMLGAERIFMVDHHAYRLRFAQEQYDVIPVN 237 Query: 217 VSQQSLVEVVQQRTEGWGADVVFEASG 243 Q E + T G G D V +A G Sbjct: 238 FDQVDPAEYIIANTAGRGVDGVIDAVG 264 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.136 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,002,674 Number of extensions: 40261 Number of successful extensions: 150 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 146 Number of HSP's successfully gapped: 5 Length of query: 345 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 259 Effective length of database: 1,212,660 Effective search space: 314078940 Effective search space used: 314078940 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 131 (55.1 bits)