BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3340.p (218 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312675|ref|YP_003776767.1| protein-glutamate methylesteras... 367 e-103 gi|300311338|ref|YP_003775430.1| chemotaxis-specific methylester... 87 2e-18 gi|300309961|ref|YP_003774053.1| chemotaxis-specific methylester... 80 1e-16 gi|300312321|ref|YP_003776413.1| protein-glutamate methylesteras... 80 2e-16 gi|300311029|ref|YP_003775121.1| protein-glutamate methylesteras... 67 9e-13 >gi|300312675|ref|YP_003776767.1| protein-glutamate methylesterase [Herbaspirillum seropedicae SmR1] Length = 211 Score = 367 bits (941), Expect = e-103, Method: Compositional matrix adjust. Identities = 184/204 (90%), Positives = 195/204 (95%) Query: 15 DLAGRSIEAIVIGASAGGVEALSEILPALRRGAAVATMVVLHIPRERPSLLASIFADRCA 74 +LA R IEAIVIGASAGGVEALSEILPALRRGAAVATMVVLH+PRERPSLLA IFADRC Sbjct: 8 ELAARPIEAIVIGASAGGVEALSEILPALRRGAAVATMVVLHLPRERPSLLAQIFADRCR 67 Query: 75 QPVVEAQDKEPAQAGVIYFAPPDYHLLVDRGEEGPLLAMSNDELVNFSRPAIDVLFESAA 134 QPVVEAQDKEPAQAGVI+FAPPDYHLLVDR E+GP+LAMSNDELVN+SRPAIDVLFESAA Sbjct: 68 QPVVEAQDKEPAQAGVIHFAPPDYHLLVDRSEDGPILAMSNDELVNYSRPAIDVLFESAA 127 Query: 135 DVYGPQLLGIVLTGGNHDGAAGLQAIARAGGVTVVQSPQQAQVPYMPAQALALGNIDYVL 194 DVYGP+LLGI+LTGGNHDGAAG+QAIARAGGVTVVQSPQQAQVPYMPAQALA+GNIDYVL Sbjct: 128 DVYGPRLLGILLTGGNHDGAAGMQAIARAGGVTVVQSPQQAQVPYMPAQALAMGNIDYVL 187 Query: 195 TLPQIADLLRTLGVTGNDQEKERR 218 TL QIADLLRTLG +G QEKERR Sbjct: 188 TLQQIADLLRTLGASGLAQEKERR 211 >gi|300311338|ref|YP_003775430.1| chemotaxis-specific methylesterase [Herbaspirillum seropedicae SmR1] Length = 359 Score = 86.7 bits (213), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 59/177 (33%), Positives = 82/177 (46%), Gaps = 1/177 (0%) Query: 20 SIEAIVIGASAGGVEALSEILPALRRGAAVATMVVLHIPRERPSLLASIFADRCAQPVVE 79 S + I+IGAS GG EA+ E L + ++ H+P A + C VVE Sbjct: 163 SEKLIIIGASTGGTEAIKEFLIRMPSDCP-GILITQHMPEGFTRSFAQRLNNLCKISVVE 221 Query: 80 AQDKEPAQAGVIYFAPPDYHLLVDRGEEGPLLAMSNDELVNFSRPAIDVLFESAADVYGP 139 + E G Y AP HL + R + + VN RP++DVLF+SAA G Sbjct: 222 SAGNERVLPGHAYIAPGHSHLKLVRSGANYMTQLDQGPPVNRHRPSVDVLFQSAATCAGK 281 Query: 140 QLLGIVLTGGNHDGAAGLQAIARAGGVTVVQSPQQAQVPYMPAQALALGNIDYVLTL 196 +G++LTG DGA G+ + AG Q V MP +A+A+G VL L Sbjct: 282 NAVGVILTGMGKDGAQGMLEMRNAGAYNFAQDEASCVVFGMPREAIAVGAAHEVLAL 338 >gi|300309961|ref|YP_003774053.1| chemotaxis-specific methylesterase [Herbaspirillum seropedicae SmR1] Length = 381 Score = 80.1 bits (196), Expect = 1e-16, Method: Compositional matrix adjust. Identities = 57/180 (31%), Positives = 88/180 (48%), Gaps = 1/180 (0%) Query: 24 IVIGASAGGVEALSEILPALRRGAAVATMVVLHIPRERPSLLASIFADRCAQPVVEAQDK 83 + IGAS GG +AL +L L + +VV H+P+ A V EA+ Sbjct: 197 VAIGASTGGPQALERVLGKLSQRCP-GIVVVQHMPQRFTRSFAERLHRISGAEVKEAEHL 255 Query: 84 EPAQAGVIYFAPPDYHLLVDRGEEGPLLAMSNDELVNFSRPAIDVLFESAADVYGPQLLG 143 + G + AP HL+V R + + LV+ +P++DVLF S A G +G Sbjct: 256 DRILPGRVLIAPGGRHLVVRREGLQYYVETLDGPLVSRHKPSVDVLFRSLAKAAGSNAVG 315 Query: 144 IVLTGGNHDGAAGLQAIARAGGVTVVQSPQQAQVPYMPAQALALGNIDYVLTLPQIADLL 203 I++TG DGA G++ +A GG T Q + V MP +A+ G + V+TL I+ ++ Sbjct: 316 IIMTGMGDDGARGMRELADCGGATYAQDEASSVVFGMPKEAIQQGGVGDVITLEHISAVI 375 >gi|300312321|ref|YP_003776413.1| protein-glutamate methylesterase [Herbaspirillum seropedicae SmR1] Length = 342 Score = 79.7 bits (195), Expect = 2e-16, Method: Compositional matrix adjust. Identities = 60/183 (32%), Positives = 95/183 (51%), Gaps = 3/183 (1%) Query: 24 IVIGASAGGVEALSEILPALRRGAAVATMVVLHIPRERPSLLASIFADRCAQPVVEAQDK 83 + +GASAGG AL+ +L A+ +G A A ++V HI +A + A V AQ+ Sbjct: 161 VAVGASAGGPAALATLLKAIPQGFAGAVVIVQHIDAAFAPGMAQWLQQQSALKVGLAQEG 220 Query: 84 EPAQAGVIYFAPPDYHLLVDRGEEGPLLAMSNDELVNFSRPAIDVLFESAADVYGPQLLG 143 + Q G + A + HL+V +EG L + RP++DV F+S + ++ G Sbjct: 221 QAPQRGAVLLAASNRHLVV---KEGGRLGYVEGAPNDVYRPSVDVFFQSIVRHWPGKVAG 277 Query: 144 IVLTGGNHDGAAGLQAIARAGGVTVVQSPQQAQVPYMPAQALALGNIDYVLTLPQIADLL 203 ++LTG DGAAGL+A+ +G T+ Q + V MP A+AL VL + +A L Sbjct: 278 LLLTGMGRDGAAGLKALRDSGAHTMAQDQKSCAVYGMPKAAVALNAAVEVLPVTGMAARL 337 Query: 204 RTL 206 + + Sbjct: 338 QAI 340 >gi|300311029|ref|YP_003775121.1| protein-glutamate methylesterase [Herbaspirillum seropedicae SmR1] Length = 353 Score = 67.4 bits (163), Expect = 9e-13, Method: Compositional matrix adjust. Identities = 58/184 (31%), Positives = 81/184 (44%), Gaps = 3/184 (1%) Query: 24 IVIGASAGGVEALSEILPALRRGAAVATMVVLHIPRERPSLLASIFADRCAQPVVEAQDK 83 ++IG S GG L EILPAL +V H+P A CA V+E Sbjct: 167 VLIGVSTGGPSTLEEILPALPASFPWPILVAQHMPAAFTRSFAERMDKLCALNVLEVAAP 226 Query: 84 EPAQAGVIYFAPPDYHLLV-DRGEEGPLLAMSNDELVNFSRPAIDVLFESA-ADVYGPQL 141 Q G +Y A +LV RG +L + + P++++L SA + QL Sbjct: 227 TVVQGGHVYIARGGADMLVAKRGSHLSVLP-KPESPTHLWHPSVELLARSALQSMAARQL 285 Query: 142 LGIVLTGGNHDGAAGLQAIARAGGVTVVQSPQQAQVPYMPAQALALGNIDYVLTLPQIAD 201 +G++LTG +DGAA I GG T+ +S V MP + G VL +IA Sbjct: 286 IGVMLTGMGNDGAAAFAEICGDGGRTIAESESSCVVFGMPKDLIDRGGASVVLPASKIAT 345 Query: 202 LLRT 205 LR Sbjct: 346 QLRV 349 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.136 0.384 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 746,250 Number of extensions: 32154 Number of successful extensions: 139 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 135 Number of HSP's successfully gapped: 5 Length of query: 218 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 136 Effective length of database: 1,231,600 Effective search space: 167497600 Effective search space used: 167497600 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 128 (53.9 bits)