BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3325.p (467 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312656|ref|YP_003776748.1| alpha-ketoglutarate permease of... 848 0.0 gi|300312423|ref|YP_003776515.1| proline/betaine transporter pro... 214 1e-56 gi|300310790|ref|YP_003774882.1| major facilitator superfamily p... 202 4e-53 gi|300311827|ref|YP_003775919.1| sugar permease of the major fac... 200 2e-52 gi|300309575|ref|YP_003773667.1| proline/betaine transporter pro... 199 3e-52 >gi|300312656|ref|YP_003776748.1| alpha-ketoglutarate permease of the major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 457 Score = 848 bits (2192), Expect = 0.0, Method: Compositional matrix adjust. Identities = 432/453 (95%), Positives = 440/453 (97%), Gaps = 2/453 (0%) Query: 17 TTASTPLSAAEYRKRIFAILGASSGNLVEWFDFYVYSFCAIYFAPAFFPKGDATSQLLNT 76 T ASTPLSAAEYRKRIFAILGASSGNLVEWFDFYVYSFCAIYFAPAFFPKGD TSQLLNT Sbjct: 5 TQASTPLSAAEYRKRIFAILGASSGNLVEWFDFYVYSFCAIYFAPAFFPKGDPTSQLLNT 64 Query: 77 AGVFAAGFLMRPIGGWLFGRIADKHGRKTSMLISVLMMCGGSLAVAIMPTYATIGAWAPA 136 AGVFAAGFLMRPIGGWLFGRIADKHGRKTSMLISVLMMCGGSLAVA+MPTYATIGAWAPA Sbjct: 65 AGVFAAGFLMRPIGGWLFGRIADKHGRKTSMLISVLMMCGGSLAVAVMPTYATIGAWAPA 124 Query: 137 LLLLARLFQGLSVGGEYGTSATYMSEVAPNGRRGFFASFQYVTLIGGQLLAVLVLFAMQQ 196 LLLLARLFQGLSVGGEYGTSATYMSEVAPNGRRGFFASFQYVTLIGGQLLAVLVLF MQQ Sbjct: 125 LLLLARLFQGLSVGGEYGTSATYMSEVAPNGRRGFFASFQYVTLIGGQLLAVLVLFGMQQ 184 Query: 197 WLSKAELMAWGWRIPFVLGAVGALVAMYLRSSLAETSSAGARKKKDAGTIKGLLQHKRAF 256 WL+KAELMAWGWR+PFVLGAVGALVAMYLRSSLAETSSAGARKKKDAGT+KGLLQHKRAF Sbjct: 185 WLTKAELMAWGWRVPFVLGAVGALVAMYLRSSLAETSSAGARKKKDAGTLKGLLQHKRAF 244 Query: 257 LNVVGFTAGGSLMFYTFTTYMQKYLVNTAGMDPKMANGVMTGALFVYMILQPIFGAISDK 316 LNVVGFTAGGSLMFYTFTTYMQKYLVNTAGMDPK+ANGVMTGALFVYMILQPIFGAISDK Sbjct: 245 LNVVGFTAGGSLMFYTFTTYMQKYLVNTAGMDPKVANGVMTGALFVYMILQPIFGAISDK 304 Query: 317 IGRRNSMLCFSFLGMVCTFPILHFLKDVSSPGVAMALAILALTIVSFYTSISGLIKAEMF 376 IGRRNSMLCF+F GMV TFPILHFLKDVSSPGVAMALAILALTIVSFYTSISGLIKAEMF Sbjct: 305 IGRRNSMLCFAFFGMVGTFPILHFLKDVSSPGVAMALAILALTIVSFYTSISGLIKAEMF 364 Query: 377 PPEVRALGVGLSYAVGNAIFGGSAEFVALSLKSVGVESAFYWYVSALCLVALIISLRMPD 436 PPEVRALGVGLSYAVGNAIFGGSAEFVALSLKS G+ESAFYWYVSALCLVALIISLRMPD Sbjct: 365 PPEVRALGVGLSYAVGNAIFGGSAEFVALSLKSAGIESAFYWYVSALCLVALIISLRMPD 424 Query: 437 PQRDGHLKGDVAMNPDEAHDDLD--TGKLHKPA 467 PQRDGHLKGDVAMNPDE + D GKLHKPA Sbjct: 425 PQRDGHLKGDVAMNPDEHQGEADAAAGKLHKPA 457 >gi|300312423|ref|YP_003776515.1| proline/betaine transporter protein [Herbaspirillum seropedicae SmR1] Length = 427 Score = 214 bits (544), Expect = 1e-56, Method: Compositional matrix adjust. Identities = 128/377 (33%), Positives = 206/377 (54%), Gaps = 15/377 (3%) Query: 34 AILGASSGNLVEWFDFYVYSFCAIYFAPAFFPKGDATSQLLNTAGVFAAGFLMRPIGGWL 93 AI+ +S GN +EWFD +Y A+ A FFP GD + LL T F F MRP+G + Sbjct: 20 AIISSSIGNALEWFDILIYGAFAVVIAKQFFPTGDDSVSLLLTFATFGVSFFMRPLGAVV 79 Query: 94 FGRIADKHGRKTSMLISVLMMCGGSLAVAIMPTYATIGAWAPALLLLARLFQGLSVGGEY 153 G +D+ GRK ++++S+++M G+ +A MP+YA+IG APA + L ++ QG S GGE+ Sbjct: 80 LGAYSDRAGRKAALMLSIMLMTVGTAMIAFMPSYASIGLLAPAGIALGKMIQGFSAGGEF 139 Query: 154 GTSATYMSEVAPNGRRGFFASFQYVTLIGGQLLAVLVLFAMQQWLSKAELMAWGWRIPFV 213 G+S ++ E AP+ RRGFF+S+Q + LLA L + L+ +L +WGWR+PF+ Sbjct: 140 GSSTAFLVEHAPH-RRGFFSSWQVASQGISLLLAALFGAVLNNMLTPEQLASWGWRVPFI 198 Query: 214 LGAVGALVAMYLRSSLAETSSAGARKKKDAGTIKGLLQHKRAFLNVVGFTAGGSLMFYTF 273 G + A +Y+R L E +K ++ H++ L + GS++ T Sbjct: 199 FGLLIAPAGIYIRRHLDEAPEFKESSEKTNAPLRDTFAHQKMRLLI----GAGSVIMATV 254 Query: 274 TTYMQKYL----VNTAGMDPKMANGVMTGALFVYMILQPIFGAISDKIGRRNSMLCFSFL 329 + Y+ Y+ V G+ + M+ A + I P+ GA+SDKIGR M+ S L Sbjct: 255 SVYLSLYIPTYAVKQLGLPAWSSFAAMSVAGLIMFIGSPLVGALSDKIGRTPFMITSSAL 314 Query: 330 GMVCTFPILHFLKDVSSPGVAMAL---AILALTIVSFYTSISGLIKAEMFPPEVRALGVG 386 +V T+P+ FL + SPG L ++ + + ++ ++ L+ A++FP R G+ Sbjct: 315 YIVLTYPMFVFLTN--SPGFLQLLLLQTVIGVLMTMYFAAMPALL-ADIFPVATRGTGMS 371 Query: 387 LSYAVGNAIFGGSAEFV 403 L+Y + IFGG A + Sbjct: 372 LAYNIAVTIFGGFAGLI 388 >gi|300310790|ref|YP_003774882.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 432 Score = 202 bits (514), Expect = 4e-53, Method: Compositional matrix adjust. Identities = 132/418 (31%), Positives = 220/418 (52%), Gaps = 20/418 (4%) Query: 29 RKRIFAILGASSGNLVEWFDFYVYSFCAIYFAPAFFPKGDATSQLLNTAGVFAAGFLMRP 88 ++ +L +SGN +E FDF++Y F A Y + FFP + L+ T G F AGFLMRP Sbjct: 10 HSKLSTVLRVTSGNFLEMFDFFLYGFYASYISKTFFPSDNEFVSLILTFGTFGAGFLMRP 69 Query: 89 IGGWLFGRIADKHGRKTSMLISVLMMCGGSLAVAIMPTYATIGAWAPALLLLARLFQGLS 148 +G + G D+ GR+ +++++ +M G+L +A +P YATIG AP L+L RL QG S Sbjct: 70 LGAIILGSYIDRVGRRQGLIVTLAIMAIGTLLIAFVPGYATIGVAAPILVLTGRLLQGFS 129 Query: 149 VGGEYGTSATYMSEVAPNGRRGFFASFQYVTLIGGQLLAVLVLFAMQQWLSKAELMAWGW 208 G E G + Y++E+A G +GF+ S+Q + + + L+ + + Q LS ++ WGW Sbjct: 130 AGVELGGVSVYLAEMATPGNKGFYVSWQSASQQAAIMASALIGYLVSQSLSPVQISDWGW 189 Query: 209 RIPFVLGAVGALVAMYLRSSLAETSSAGARKKKDA--GTIKGLLQHKRAFLNVVGFTAGG 266 RIPF +G + +R SL ET ARK+ + + + Q+ + + + Sbjct: 190 RIPFFIGCMIVPAIFIIRRSLQETDEFLARKRHPSFGEIMSSIAQNWKIVVAGMMMVVMT 249 Query: 267 SLMFYTFTTYMQ---KYLVNTAGMDPKMANGVMTGALFVYMILQPIFGAISDKIGRRNSM 323 ++ FY T Y K ++ + D + + + F ++ PI GA+SD++GRR + Sbjct: 250 TVSFYLITVYTPTFGKNVLKLSASDSLIVTFCVGLSNFCWL---PIMGALSDRVGRRPVL 306 Query: 324 LCFSFLGMVCTFPILHFL-KDVSSPGVAMALAILALTIVSFYTSISG---LIKAEMFPPE 379 + F+ L ++ +P++ +L D+S L I L + Y S +G + E+ P Sbjct: 307 VAFTLLTLLTAYPVMSWLVSDISFKN----LLITELWLSFLYASYNGAAVVALTEVMPAH 362 Query: 380 VRALGVGLSYAVGNAIFGGSAEFVALSLKSVGVESAF--YWY-VSALC-LVALIISLR 433 VR +G L+Y++ AIFGG V+ L + A YW +A+C L+A ++ R Sbjct: 363 VRTVGFSLAYSLATAIFGGFTPLVSTWLIETSGDKAAPGYWMSFAAVCGLLATLVLYR 420 >gi|300311827|ref|YP_003775919.1| sugar permease of the major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 428 Score = 200 bits (509), Expect = 2e-52, Method: Compositional matrix adjust. Identities = 127/414 (30%), Positives = 222/414 (53%), Gaps = 12/414 (2%) Query: 29 RKRIFAILGASSGNLVEWFDFYVYSFCAIYFAPAFFPKGDATSQLLNTAGVFAAGFLMRP 88 + + +L +SGN +E FDF+++ F A + + AFFP + + L+ T F AGFLMRP Sbjct: 8 QSKFSTVLRVTSGNFMEMFDFFLFGFYATHISKAFFPSDNEFASLMLTFMTFGAGFLMRP 67 Query: 89 IGGWLFGRIADKHGRKTSMLISVLMMCGGSLAVAIMPTYATIGAWAPALLLLARLFQGLS 148 +G + G D+ GR+ +++++ +M G++ +A +P+YATIGA AP L+L+ RL QG S Sbjct: 68 LGAIILGAYVDRVGRRQGLIVTLALMALGTMLIAFVPSYATIGALAPLLVLIGRLLQGFS 127 Query: 149 VGGEYGTSATYMSEVAPNGRRGFFASFQYVTLIGGQLLAVLVLFAMQQWLSKAELMAWGW 208 G E G + Y++E+A G +GF+ S+Q + ++A + + + L+ AE+ WGW Sbjct: 128 AGVELGGVSVYLAEMATPGNKGFYVSWQSASQQVAIIVAAAIGYLLSTSLTPAEVSGWGW 187 Query: 209 RIPFVLGAVGALVAMYLRSSLAETSSAGARKKK-DAGTI-KGLLQHKRAFLNVVGFTAGG 266 RIPF +G + V +R SL ET RK + G I + ++++ + + + + Sbjct: 188 RIPFFIGCLIVPVLFVIRRSLQETEEFMRRKHRPSTGQIFRSMIENWKLVIAGMMLVSMT 247 Query: 267 SLMFYTFTTYMQKYLVNTAGMDPKMANGVMTGALFVYMILQPIFGAISDKIGRRNSMLCF 326 ++ FY T Y + N + + + V I PI GA+SD++GRR ++ F Sbjct: 248 TVSFYLITVYTPTFGKNVLKLSTEASLIVTLCVGLSNFIWLPIMGALSDRVGRRPLLVTF 307 Query: 327 SFLGMVCTFPILHFLKDVSSPGVAMALAILALTIVSFYTSISGLIKA---EMFPPEVRAL 383 + L ++ +P +++L +S + I+ L + Y S +G + E+ P +VR Sbjct: 308 TVLTILTAYPAVNWLVHDASFA---KMLIVELWLSFLYASYNGAMVVALTEVMPADVRTA 364 Query: 384 GVGLSYAVGNAIFGGSAEFVALSLKSVGVESA---FYWYVSALC-LVALIISLR 433 G L+Y++ A+FGG +A L + A + +A+C L+A +I R Sbjct: 365 GFSLAYSLATAVFGGFTPAIATGLIEYTHDKAAPGIWMSTAAVCGLIATLILYR 418 >gi|300309575|ref|YP_003773667.1| proline/betaine transporter protein [Herbaspirillum seropedicae SmR1] Length = 433 Score = 199 bits (507), Expect = 3e-52, Method: Compositional matrix adjust. Identities = 131/420 (31%), Positives = 221/420 (52%), Gaps = 16/420 (3%) Query: 27 EYRKRIFAILGASSGNLVEWFDFYVYSFCAIYFAPAFFPKGDATSQLLNTAGVFAAGFLM 86 + R+R AI+ GN +EWFDF VYSF A+ A FFP G+ + LL F GF M Sbjct: 17 QQRRR--AIVATVIGNGLEWFDFTVYSFFAVIIAKLFFPTGNDLTSLLLAVATFGVGFFM 74 Query: 87 RPIGGWLFGRIADKHGRKTSMLISVLMMCGGSLAVAIMPTYATIGAWAPALLLLARLFQG 146 RP+GG L G AD+ GRK ++ +++L+M G+ + + PTY +IG +AP ++++ARL QG Sbjct: 75 RPVGGILLGIYADRVGRKAALSVTILLMAVGTTMIGLAPTYESIGLFAPLIIVVARLLQG 134 Query: 147 LSVGGEYGTSATYMSEVAPNGRRGFFASFQYVTLIGGQLLAVLVLFAMQQWLSKAELMAW 206 S GGE G++ +++E AP R +++S+ ++ LL V + L A L +W Sbjct: 135 FSAGGEMGSATAFLTEYAPERERAYYSSWIQASIGVAVLLGAAVGTFVTASLDAAALASW 194 Query: 207 GWRIPFVLGAVGALVAMYLRSSLAETSSAGARKKKDAGTIKGLLQH-----KRAFLNVVG 261 GWR+PF+LG + V Y+R L ET + K K +K ++++ +F V+ Sbjct: 195 GWRLPFLLGIIIGPVGYYIRHHLEETPTFLEEKDKTDSPLKEVIRNFPRETSASFSMVIL 254 Query: 262 FTAGGSLMFYTFTTYMQKYLV--NTAGMDPKMANGVMTGALFVYMILQPIFGAISDKIGR 319 +T ++ + TY K L T G M G+ M+L PI G ++D+IGR Sbjct: 255 WTVCTYVLLFYMPTYSVKVLKLPQTTGFIAGMVGGLCI------MVLAPIVGRLADRIGR 308 Query: 320 RNSMLCFSFLGMVCTFPILHFLKDVSSPGVAMALAILALTIVSFYTSISGLIKAEMFPPE 379 R + + L ++ +P+ ++ S + ++ T+++ YT +E+FP + Sbjct: 309 RPLLSGSAALILMLAYPMFAYINSAPSLSTLLIFQLVFGTLIAAYTGPILAAFSELFPAK 368 Query: 380 VRALGVGLSYAVGNAIFGGSAE-FVALSLKSVGVESAFYWYVSALCLVALIISLRMPDPQ 438 V + G+ ++Y + IFGG A F+ + + G A +YV ++ + + + +PQ Sbjct: 369 VLSTGLSVAYNLAVTIFGGFASFFITWLIAATGSTMAPAFYVMIAAAISFVGTRFVREPQ 428 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.325 0.137 0.415 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,359,041 Number of extensions: 55320 Number of successful extensions: 332 Number of sequences better than 1.0e-08: 16 Number of HSP's gapped: 296 Number of HSP's successfully gapped: 16 Length of query: 467 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 378 Effective length of database: 1,198,455 Effective search space: 453015990 Effective search space used: 453015990 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 132 (55.5 bits)