BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3318.p (343 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312649|ref|YP_003776741.1| oxidoreductase protein [Herbasp... 642 0.0 gi|300309662|ref|YP_003773754.1| aryl-alcohol dehydrogenase [Her... 140 2e-34 gi|300312028|ref|YP_003776120.1| oxidoreductase protein [Herbasp... 124 1e-29 gi|300313828|ref|YP_003777920.1| aryl-alcohol dehydrogenase [Her... 123 2e-29 gi|300312019|ref|YP_003776111.1| aryl-alcohol dehydrogenase [Her... 112 4e-26 >gi|300312649|ref|YP_003776741.1| oxidoreductase protein [Herbaspirillum seropedicae SmR1] Length = 343 Score = 642 bits (1655), Expect = 0.0, Method: Compositional matrix adjust. Identities = 310/342 (90%), Positives = 326/342 (95%) Query: 1 MSAPITSHLQPDSPVGSRRLGSNGPLVSAIGLGCMGMSDFYARRDDAESLATIDRALELG 60 MS P+TSHLQ SPVG+RRLGSNGPLVSAIGLGCMGMSDFYA+RDDAESLATIDRALELG Sbjct: 1 MSEPLTSHLQAHSPVGTRRLGSNGPLVSAIGLGCMGMSDFYAQRDDAESLATIDRALELG 60 Query: 61 INLLDTADMYGPYTNEELLGRAIQGRRDKFFIATKFGIRRDPADPQARGVDGSPAYIRQA 120 INLLDTADMYGPYTNEELLGRAI+GRRDKFFIATKFGIRRDP+DP ARGVDGSPAYIRQA Sbjct: 61 INLLDTADMYGPYTNEELLGRAIKGRRDKFFIATKFGIRRDPSDPGARGVDGSPAYIRQA 120 Query: 121 VEGSLRRLGVDTIDLYYQHRIDPATPIEATMGVLADLVRAGKIRYIGLSEASAATLERAH 180 VEGSL+RLGV+TIDLYYQHRIDPATPIE TMGVLADLV+AGKIRYIGLSEASAATLERAH Sbjct: 121 VEGSLKRLGVETIDLYYQHRIDPATPIEVTMGVLADLVQAGKIRYIGLSEASAATLERAH 180 Query: 181 RVHPVTALQSEYSLWTRDPEAEVLATCRALGIGFVAYSPLGRGFLTGAIQRFEDLAEDDF 240 RVHPVTALQSEYSLWTRDPEAEVLA CR LGIGFVAYSPLGRGFLTGAIQRFEDLAEDDF Sbjct: 181 RVHPVTALQSEYSLWTRDPEAEVLAACRTLGIGFVAYSPLGRGFLTGAIQRFEDLAEDDF 240 Query: 241 RRTNPRFQGENFARNLRLVDKVKEMAEHHGCTPSQLALAWVMAQDPHIVPIPGTKRRRYL 300 RR+NPRFQGENFARNL+L +KVKEMA HGCTPSQLALAWVMAQDPHIVPIPGTKRRRYL Sbjct: 241 RRSNPRFQGENFARNLQLAEKVKEMAGQHGCTPSQLALAWVMAQDPHIVPIPGTKRRRYL 300 Query: 301 EENAGAFNVKLSSEDLLALEAIFPRGVAAGARYTEASMKMLN 342 E+NAG+ VKL+ EDL AL+AIFPRG AAG RYT+ASMKMLN Sbjct: 301 EDNAGSVGVKLAPEDLQALDAIFPRGAAAGERYTDASMKMLN 342 >gi|300309662|ref|YP_003773754.1| aryl-alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 335 Score = 140 bits (353), Expect = 2e-34, Method: Compositional matrix adjust. Identities = 102/330 (30%), Positives = 159/330 (48%), Gaps = 22/330 (6%) Query: 18 RRLGSNGPLVSAIGLGCMGMSDFYARRDDAESLATIDRALELGINLLDTADMYGPYTNEE 77 RRLG + VSA+ LG M D + D ES + A E G+N +DTAD+Y +EE Sbjct: 4 RRLGKSNLKVSALCLGSMMFGD---QTDLQESGRIVASAREHGVNFIDTADVYTKGKSEE 60 Query: 78 LLGRAIQGRRDKFFIATKFGIRRDPADPQARGVDGSPAYIRQAVEGSLRRLGVDTIDLYY 137 ++G+ + G+R ++ +A+K G + + + S ++I + E L RL D +D+ Y Sbjct: 61 IVGKLLAGQRHEWVLASKLG---NAMSGKPNESNYSRSWIVRETENILTRLDTDYLDILY 117 Query: 138 QHRIDPATPIEATMGVLADLVRAGKIRYIGLSE------ASAATLERAHRVHPVTALQSE 191 HR +E + L DL+R GKIR G+S A L + V Q Sbjct: 118 MHRDYQDENLEEAVQALGDLIRVGKIRGFGVSNFRGWRIAEIVRLCEKNNVPLPLVCQPY 177 Query: 192 YSLWTRDPEAEVLATCRALGIGFVAYSPLGRGFLTGAIQRFEDLAED------DFRRTNP 245 Y+L R PE E+L C G+G V YSP+ RG LTG Q + A+D D R Sbjct: 178 YNLLNRQPEVEILPACAHYGLGVVPYSPIARGVLTGKYQPGQKPAQDTRAGRGDKRMMET 237 Query: 246 RFQGENFARNLRLVDKVKEMAEHHGCTPSQLALAWVMAQDPHIVPIPGTKRRRYLEENAG 305 F+ E +L + ++++ E G Q A AWV+A I G + +E+ Sbjct: 238 EFREE----SLLIAQRLQQHCEARGVKLGQFATAWVLANSAISSVIAGPRTLAQMEDYYD 293 Query: 306 AFNVKLSSEDLLALEAIFPRGVAAGARYTE 335 A +K+S+E+ ++++ G A+ Y + Sbjct: 294 AAELKISAEEEALVDSLVTPGHASTHGYND 323 >gi|300312028|ref|YP_003776120.1| oxidoreductase protein [Herbaspirillum seropedicae SmR1] Length = 349 Score = 124 bits (310), Expect = 1e-29, Method: Compositional matrix adjust. Identities = 116/348 (33%), Positives = 165/348 (47%), Gaps = 48/348 (13%) Query: 18 RRLGSNGPLVSAIGLGCMGMSDFYARRDDAESLATIDRALELGINLLDTADMY------- 70 R LG VS I LG M + + +A++ A +D A+E GINL+D A+MY Sbjct: 4 RPLGRTDLKVSLITLGTMTWGE---QNSEADAHAQLDYAVERGINLIDVAEMYPVPPKAE 60 Query: 71 GPYTNEELLGRAIQ--GRRDKFFIATKF-GIRRDPADPQARGVDGSPAYI-----RQAVE 122 E LG ++ G+RD+ IATK G R P +P R V G + +A+ Sbjct: 61 TQGLTERYLGTWLKKSGKRDQLLIATKCTGPARKPHNP--RHVRGGINNLDRKGLTEALH 118 Query: 123 GSLRRLGVDTIDLY----------------YQHRIDPAT-PIEATMGVLADLVRAGKIRY 165 GSL RL +D +DLY YQH D +T PIE T+ VL++ V+AGKIR Sbjct: 119 GSLERLQLDHVDLYQLHWPDRSVNSFGQLGYQHVEDESTVPIEETLTVLSEFVKAGKIRT 178 Query: 166 IGLSEASA---ATLERA---HRVHPVTALQSEYSLWTRDPEAEVLATCRALGIGFVAYSP 219 IGLS +A A RA H + + +Q+ Y+L R E + +G +AYSP Sbjct: 179 IGLSNETAWGVAQFLRAAEQHGLERIVTIQNPYNLLNRSFEINLAEFAHREQVGLLAYSP 238 Query: 220 LGRGFLTGAIQRFEDLAEDDFRRTN--PRFQGENFARNLRLVDKVKEMAEHHGCTPSQLA 277 L G L+G ++ D A R RF A+ R + +A HG P+Q+A Sbjct: 239 LAFGMLSG---KYLDGARPAGGRLTLFERFSRYTNAQAERATAEYVALARQHGLDPAQMA 295 Query: 278 LAWVMAQDPHIVPIPGTKRRRYLEENAGAFNVKLSSEDLLALEAIFPR 325 LA+V ++ I G L N + ++L + L A+EAI R Sbjct: 296 LAYVNSRPFLTSNIIGATTLAQLRSNIDSAELQLGEDVLAAIEAIHRR 343 >gi|300313828|ref|YP_003777920.1| aryl-alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 344 Score = 123 bits (308), Expect = 2e-29, Method: Compositional matrix adjust. Identities = 100/322 (31%), Positives = 160/322 (49%), Gaps = 31/322 (9%) Query: 18 RRLGSNGPLVSAIGLGCM----GMSDFYARRDDAESLATIDRALELGINLLDTADMYGP- 72 RR G +G L+ AI LG G+ +F R A + RA + G+ D A+ YGP Sbjct: 17 RRTGRSGLLLPAISLGLWQNFGGVDNFETGR------AVLRRAFDRGVTHFDLANNYGPP 70 Query: 73 -YTNEELLGRAIQG----RRDKFFIATKFGIRRDPADPQARGVDGSPAYIRQAVEGSLRR 127 + EE GR + RD+ I++K G + P P G G+ ++ + E SL+R Sbjct: 71 AGSAEENFGRWMAKDFLPYRDELVISSKAGWQMWPG-PYG-GPSGARKHLIASCEQSLKR 128 Query: 128 LGVDTIDLYYQHRIDPATPIEATMGVLADLVRAGKIRYIGLSEAS------AATLERAHR 181 +G+D +D++Y HR+D TP+E TMG LA L R GK Y+G+S S AA + ++ Sbjct: 129 MGLDYVDIFYSHRVDADTPLEETMGALAHLHRQGKALYVGISSYSPELTRKAAAILKSEG 188 Query: 182 VHPVTALQSEYSLWTRDPEAEVLATCRALGIGFVAYSPLGRGFLTGAIQRFEDLAEDDFR 241 V P+ Q YS+ R E +L LG+G + +SPL +G LT ++ + R Sbjct: 189 V-PLLIHQPNYSMLNRAIEGGLLPALEELGVGCIGFSPLAQGLLT---SKYLGGVPEGAR 244 Query: 242 RTN-PRFQGENFAR-NLRLVDKVKEMAEHHGCTPSQLALAWVMAQDPHIVPIPGTKRRRY 299 T P + NL + + E+A G T +Q+A+AWV+ + G + Sbjct: 245 VTRAPSLPQRMLSEDNLARIRALNEIASKRGQTLAQMAIAWVLRDARVTSALIGARTVEQ 304 Query: 300 LEENAGAFN-VKLSSEDLLALE 320 L+++ A N + + E+L +++ Sbjct: 305 LDDSLDAVNKLDFTQEELASID 326 >gi|300312019|ref|YP_003776111.1| aryl-alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 329 Score = 112 bits (280), Expect = 4e-26, Method: Compositional matrix adjust. Identities = 94/326 (28%), Positives = 155/326 (47%), Gaps = 20/326 (6%) Query: 17 SRRLGSNGPLVSAIGLGCMGMSDFYARRDDAESLATIDRALELGINLLDTADMYGPYTNE 76 SRR G G VS IG G + + ++ A + AL+ G++ +DTAD+YG +E Sbjct: 3 SRRFGRTGAQVSEIGFGAWAIGGSWGDVSAQDAKAALHAALDAGVSFIDTADVYGDGRSE 62 Query: 77 ELLGRAIQGR-RDKFFIATKFGIRRDPADPQARGVDGSPAYIRQAVEGSLRRLGVDTIDL 135 +L+ + ++ R ++ F+ATK G R P A G + A + ++ SL+ LGVD +DL Sbjct: 63 QLIAQVLKERGGERPFVATKAGRRLSP--HVASGYNDR-AQLSAFIDRSLKNLGVDCLDL 119 Query: 136 YYQHRIDPATPI---EATMGVLADLVRAGKIRYIGLSEASAATLERAHRVHPVTALQSEY 192 H P T + + G L LV AGKIR+ G+S +A V ++Q + Sbjct: 120 VQLH--CPPTEVYYQQEVFGHLDALVAAGKIRHYGVSVEKVEEALKAIEYPNVKSVQIIF 177 Query: 193 SLWTRDPEAEVLATCRALGIGFVAYSPLGRGFLTGAIQRFEDLAEDDFRRTN-------- 244 +++ + P + L + I +A PL G LTG + A DD R+ N Sbjct: 178 NIFRQRPISLFLQEAQRRDIAVIARVPLASGLLTGKMSAQTTFAADDHRQFNRHGEAFDV 237 Query: 245 -PRFQGENFARNLRLVDKVKEMAEHHGCTPSQLALAWVMAQDPHIVPIPGTKRRRYLEEN 303 F G + L V+K++ + G +Q AL W++ + IPG + + N Sbjct: 238 GETFSGVPYEVALAAVEKIRALVP-QGVPMAQFALRWILMESGISTVIPGARNVAQAQSN 296 Query: 304 AGAFNV-KLSSEDLLALEAIFPRGVA 328 + A + +++E + AL AI+ +A Sbjct: 297 SAASALPPITAEVMQALRAIYLHDIA 322 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.137 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,126,673 Number of extensions: 49048 Number of successful extensions: 153 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 137 Number of HSP's successfully gapped: 6 Length of query: 343 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 257 Effective length of database: 1,212,660 Effective search space: 311653620 Effective search space used: 311653620 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)