BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3313.p (332 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312643|ref|YP_003776735.1| Zn-dependent protease with chap... 543 e-155 gi|300309997|ref|YP_003774089.1| Zn-dependent protease [Herbaspi... 78 8e-16 gi|300312597|ref|YP_003776689.1| Zn-dependent protease with chap... 62 5e-11 >gi|300312643|ref|YP_003776735.1| Zn-dependent protease with chaperone function transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 329 Score = 543 bits (1398), Expect = e-155, Method: Compositional matrix adjust. Identities = 279/332 (84%), Positives = 300/332 (90%), Gaps = 3/332 (0%) Query: 1 MTISATYYDGKSSRPHQVTLQVSNGVAQLRGEVERECPIAELRVSERFTRAARKVSFPDG 60 M++ A+YYDG++SR HQVTL+V GVAQ+RGE+ER+CPI +LRVSERF RAARKVSFPDG Sbjct: 1 MSVLASYYDGRTSRRHQVTLEVVAGVAQVRGEIERDCPIGQLRVSERFARAARKVSFPDG 60 Query: 61 AYLEVTDGPAFSALLQETGHRDSLVVRLQHSWRHALAACVALVAVVILSYLYLLPLAAQG 120 AYLE+ DG AF LLQETGH+DSLVVRLQ SWRHALAAC LVAVV+LSY+YLLPLAAQG Sbjct: 61 AYLEIIDGAAFRELLQETGHQDSLVVRLQQSWRHALAACAVLVAVVVLSYVYLLPLAAQG 120 Query: 121 IVAVLPVEVDRQVGRGTLEFLDSHVLSKSQLDGRRAAAITARFKALTAPMDDAPEYQIVF 180 I A LPV VDRQ+G+GTLEFLD HVL+ SQLD RR AITARF+ALTAP+ DAP+YQIVF Sbjct: 121 IAATLPVAVDRQIGQGTLEFLDQHVLAPSQLDARRTQAITARFRALTAPLPDAPDYQIVF 180 Query: 181 RKSRIGPNAFALPSGQIVVTDEIVNLLDDDDALMGVLAHELGHVHRRHLMRRLIQSSAIA 240 RKSRIGPNAFALPSGQIVVTDEIV LLDDDDALMGVLAHELGHVHRRHLMRRLIQSSAIA Sbjct: 181 RKSRIGPNAFALPSGQIVVTDEIVRLLDDDDALMGVLAHELGHVHRRHLMRRLIQSSAIA 240 Query: 241 AAATVLFGDVSAVLANIPTVLLDLKYSRDIEREADDYAIAMFKANGLPRQKLALVFEKLE 300 A ATVLFGDVSAVLANIPTVLLDLKYSRDIEREADDYAIAMFKANGLPRQKLALVFEKLE Sbjct: 241 ATATVLFGDVSAVLANIPTVLLDLKYSRDIEREADDYAIAMFKANGLPRQKLALVFEKLE 300 Query: 301 QVGEDHKDGPALPYLSSHPANEERIAHILGTR 332 G KD PA PYLSSHPAN+ERIAHILG R Sbjct: 301 SAG---KDDPASPYLSSHPANDERIAHILGAR 329 >gi|300309997|ref|YP_003774089.1| Zn-dependent protease [Herbaspirillum seropedicae SmR1] Length = 552 Score = 78.2 bits (191), Expect = 8e-16, Method: Compositional matrix adjust. Identities = 57/156 (36%), Positives = 77/156 (49%), Gaps = 16/156 (10%) Query: 188 NAFALPSGQIVVTDEIVNLLDDDDALMGVLAHELGHVHRRHLMRRL---IQSSAIAAAAT 244 NAFALP G I V +V + L GV++HE+GHV +RH+ R L Q+S I AA Sbjct: 158 NAFALPGGFIGVNSGLVLAAQSESELAGVMSHEIGHVAQRHIARMLGQQKQNSMIQLAAI 217 Query: 245 VL--FGDVS-----AVLANIP-----TVLLDLKYSRDIEREADDYAIAMFKANGLPRQKL 292 VL VS A +A + V L +SRD EREAD + + + G + Sbjct: 218 VLGALAGVSKAGGDAAMATMMGGTGLAVQRQLNFSRDAEREADRIGLQIMRDGGFDPSGM 277 Query: 293 ALVFEKLEQVGEDHKDGPALPYLSSHPANEERIAHI 328 F +L+ ++ D PYL +HP ERIA I Sbjct: 278 VTFFGRLQAASRNYSDA-VPPYLMTHPLTTERIADI 312 >gi|300312597|ref|YP_003776689.1| Zn-dependent protease with chaperone function protein [Herbaspirillum seropedicae SmR1] Length = 273 Score = 62.4 bits (150), Expect = 5e-11, Method: Compositional matrix adjust. Identities = 45/148 (30%), Positives = 73/148 (49%), Gaps = 11/148 (7%) Query: 188 NAFALPSGQIVV-TDEIVNLLDDDDALMGVLAHELGHVHRRHLMRR----LIQSSAIAAA 242 NA+ +P G+I V T I L DD L V+ HE+ H R H R ++ +SAI+ Sbjct: 113 NAWCMPGGKIAVYTGLIEKLKISDDELAAVMGHEIAHALREHARERASQQVVANSAISIG 172 Query: 243 ATVL-FGDVSAVLAN-IPTVLLDLKYSRDIEREADDYAIAMFKANGLPRQKLALVFEKLE 300 A +L GDV + L+ L SR E EAD + + G + +++K+ Sbjct: 173 AALLGLGDVGKQGGQYLYMGLMGLPNSRANETEADRIGVELAARGGYDPKAAVTLWQKMA 232 Query: 301 QVGEDHKDGPALPYLSSHPANEERIAHI 328 +G + + ++S+HP++ ERIA + Sbjct: 233 SLGGEE----PMKFMSTHPSSSERIADL 256 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.135 0.384 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,001,236 Number of extensions: 40432 Number of successful extensions: 147 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 144 Number of HSP's successfully gapped: 3 Length of query: 332 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 246 Effective length of database: 1,212,660 Effective search space: 298314360 Effective search space used: 298314360 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 131 (55.1 bits)