BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3295.p (400 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312624|ref|YP_003776716.1| acetylornithine aminotransferas... 793 0.0 gi|300310415|ref|YP_003774507.1| 4-aminobutyrate aminotransferas... 165 8e-42 gi|300312816|ref|YP_003776908.1| aminotransferase class III prot... 144 1e-35 gi|300313229|ref|YP_003777321.1| 4-aminobutyrate aminotransferas... 142 7e-35 gi|300310456|ref|YP_003774548.1| family II aminotransferase [Her... 129 4e-31 >gi|300312624|ref|YP_003776716.1| acetylornithine aminotransferase [Herbaspirillum seropedicae SmR1] Length = 400 Score = 793 bits (2049), Expect = 0.0, Method: Compositional matrix adjust. Identities = 385/400 (96%), Positives = 391/400 (97%) Query: 1 MQYSQSDANKLMYVTNRPELVFTEGQGMWLTDHHGKRYLDYLQGWAVNTLGHAPQCIADA 60 MQYSQSDANKLMYVTNRPELVFTEG GMWLTDH+GKRYLDYLQGWAVNTLGHAPQCIADA Sbjct: 1 MQYSQSDANKLMYVTNRPELVFTEGHGMWLTDHNGKRYLDYLQGWAVNTLGHAPQCIADA 60 Query: 61 LAAQSKKLINPSPAFYNAPSMELAKLLTDNSVFDRVFFANSGAEANEGAIKLARKWGKKN 120 LAAQSKKLINPSPAFYN PS+ELAKLLT NSVFDRVFFANSG EANEGAIKLARKWGKKN Sbjct: 61 LAAQSKKLINPSPAFYNEPSIELAKLLTANSVFDRVFFANSGGEANEGAIKLARKWGKKN 120 Query: 121 PAGDGSARFEIITFKHSFHGRTLATMSASGKDGWDTMFAPQVPGFPKATLNDLASVEALI 180 PA DGSARFEIITFKHSFHGRTLATMSASGKDGWDTMFAPQVPGFPKA LNDL SV+ALI Sbjct: 121 PAADGSARFEIITFKHSFHGRTLATMSASGKDGWDTMFAPQVPGFPKAVLNDLESVKALI 180 Query: 181 GSHTVAVMLEPVQGEGGVIPASKEFMQGLRRLTKEKNLLLIVDEVQSGMGRTGQLFAYQH 240 G HTVAVMLEPVQGEGGVIPASKEFMQGLR LTKEKNLLLIVDEVQSGMGRTGQLFAYQH Sbjct: 181 GEHTVAVMLEPVQGEGGVIPASKEFMQGLRSLTKEKNLLLIVDEVQSGMGRTGQLFAYQH 240 Query: 241 SGIEPDIMTLAKGIGGGVPLAALLAREEIACFEAGEQGGTYNGNPLMTAVGVAVIKELLK 300 SGIEPDIMTLAKGIGGGVPLAALLAREEIACFEAGEQGGTYNGNPLMTAVGVAVIKELLK Sbjct: 241 SGIEPDIMTLAKGIGGGVPLAALLAREEIACFEAGEQGGTYNGNPLMTAVGVAVIKELLK 300 Query: 301 PGFMETVRERGQYLRQRSLEISEKYGFEGERGEGLLRAMQLGRDIGPQIVEAARNLEPVG 360 PGFME+VRERGQYLRQRSLEISEKYGFEGERGEGLLRA+QLGRDIGPQIVEAARNLEPVG Sbjct: 301 PGFMESVRERGQYLRQRSLEISEKYGFEGERGEGLLRALQLGRDIGPQIVEAARNLEPVG 360 Query: 361 LLLNSPRPNLLRFMPALNVSKEEIDQMFSMLEEVLAKIGK 400 LLLNSPRPNLLRFMPALNV+KEEIDQMFSMLEEVLAKIGK Sbjct: 361 LLLNSPRPNLLRFMPALNVTKEEIDQMFSMLEEVLAKIGK 400 >gi|300310415|ref|YP_003774507.1| 4-aminobutyrate aminotransferase [Herbaspirillum seropedicae SmR1] Length = 426 Score = 165 bits (417), Expect = 8e-42, Method: Compositional matrix adjust. Identities = 133/397 (33%), Positives = 196/397 (49%), Gaps = 40/397 (10%) Query: 28 MWLTDHHGKRYLDYLQGWAVNTLGHAPQCIADALAAQSKKLINPSPAFY-NAPSMELAKL 86 +W D G+R++D+ G AV GH + DA+ AQ K + + A +ELA+ Sbjct: 38 LW--DVEGRRFIDFAAGIAVLNTGHRHPKLLDAMRAQMDKFTHTAYQIVPYASYVELAER 95 Query: 87 ---LTDNSVFDRVFFANSGAEANEGAIKLARKWGKKNPAGDGSARFEIITFKHSFHGRTL 143 LT + + F ++GAEA E AIK+AR + R +I F FHGRT+ Sbjct: 96 INRLTPGNYPKKTAFFSTGAEAVENAIKIARAH---------TGRPGVIAFAGGFHGRTM 146 Query: 144 ATMSASGKDG----------WDTMFAPQVPGFPKATLND-LASVEALIGS-----HTVAV 187 M+ +GK D AP T D L +V+ L S A+ Sbjct: 147 MGMALTGKVAPYKLGFGPFPGDVFHAPYPSALHGITSEDALEAVKGLFKSDIEAKRVAAI 206 Query: 188 MLEPVQGEGGVIPASKEFMQGLRRLTKEKNLLLIVDEVQSGMGRTGQLFAYQHSGIEPDI 247 +LEPVQGEGG A +FM+GLR L E +LLI DEVQSG GRTG+LFA +H + PD+ Sbjct: 207 ILEPVQGEGGFYAAPADFMRGLRALCDEHGILLIADEVQSGYGRTGKLFAMEHYDVLPDL 266 Query: 248 MTLAKGIGGGVPLAALLAREEIACFEA-GEQGGTYNGNPLMTAVGVAVIKELLKPGFMET 306 MT+AK + GG+PL+A+ R EI A G GGTY GNPL A +AV+ + + + Sbjct: 267 MTMAKSLAGGMPLSAVNGRAEIMDAPAPGGLGGTYAGNPLAIASALAVLDVMEEEQLVTR 326 Query: 307 VRERGQYLRQRSLEISEKYGFEGE-RGEGLLRAMQL-----GRDIGPQIVEAARNLEPVG 360 + G L++ E+ E RG G + A++ G+ + ++ G Sbjct: 327 GQRLGDKLQEHLKELRSSVPQIAEVRGVGAMVAVEFADPATGKPDAEYTKKVQQHALNNG 386 Query: 361 LLLNS--PRPNLLRFMPALNVSKEEIDQMFSMLEEVL 395 LLL + N++RF+ L + +D+ +L + + Sbjct: 387 LLLLTCGSYGNVIRFLFPLTIPDTVMDEALGILAKAI 423 >gi|300312816|ref|YP_003776908.1| aminotransferase class III protein [Herbaspirillum seropedicae SmR1] Length = 440 Score = 144 bits (363), Expect = 1e-35, Method: Compositional matrix adjust. Identities = 124/426 (29%), Positives = 199/426 (46%), Gaps = 62/426 (14%) Query: 25 GQGMWLTDHHGKRYLDYLQGWAVNTLGHAPQCIADALAAQSKKLINPSPAFYN-APSMEL 83 G+GM L D GKRY+D G AV+ LGH + +A+ Q +L +F+ AP+ EL Sbjct: 19 GEGMELIDQDGKRYIDASGGAAVSCLGHGHPRVIEAIRKQVGELAYAHTSFFTTAPAEEL 78 Query: 84 AKLLTDNS--VFDRVFFANSGAEANEGAIKLARKWGKK--NPAGDGSARFEIITFKHSFH 139 A +L D + + V+F + G+EA E A+KLAR++ + P R II + S+H Sbjct: 79 AAMLADAAPGSLNHVYFLSGGSEAVEAALKLARQYYVEVGQPQ-----RRHIIARRQSYH 133 Query: 140 GRTLATMSASGKDGWDTMFAPQV-------PGFPKATLNDLAS---------------VE 177 G TL ++ G MF P + P + D S + Sbjct: 134 GNTLGALAIGGNAWRREMFMPMLIEAHHVSPCYAYRNRADGESDVQYVQRLADELEQKIL 193 Query: 178 ALIGSHTVAVMLEPVQG-EGGVIPASKEFMQGLRRLTKEKNLLLIVDEVQSGMGRTGQLF 236 +L +A + E V G G +P ++ + +R + + +LLI+DEV SGMGRTG LF Sbjct: 194 SLGADQVIAFVAETVVGATAGAVPPVGDYFRKIRAVCDKYGVLLILDEVMSGMGRTGYLF 253 Query: 237 AYQHSGIEPDIMTLAKGIGGGV-PLAALLAREEI--ACFEAG---EQGGTYNGNPLMTAV 290 A + G+ PDI+ +AKG+G G P+ A++ + I A + G TY G+ A Sbjct: 254 ACEEDGVVPDIVVIAKGLGAGYQPIGAMICSDHIYDAVLRGSGFFQHGHTYIGHATACAA 313 Query: 291 GVAVIKELLKPGFMETVRERGQYLRQRSLEISEKYGFEGE----RGEGLLRAMQLGRD-- 344 VAV K + + +E VR+RG+ LR E+ + +G + RG GL ++L + Sbjct: 314 AVAVQKTIQEERLLENVRQRGEQLRS---ELRQAFGDQAHVGDIRGRGLFVGVELVAERS 370 Query: 345 ----IGPQIVEAARN----------LEPVGLLLNSPRPNLLRFMPALNVSKEEIDQMFSM 390 + P + AR + P+G ++ + + P S +I ++ Sbjct: 371 SKLPLSPDLRTHARVKAEAMKRGLLVYPMGGTIDGKNGDHILLAPPFIASSNDISEIVQR 430 Query: 391 LEEVLA 396 L + +A Sbjct: 431 LSDAVA 436 >gi|300313229|ref|YP_003777321.1| 4-aminobutyrate aminotransferase [Herbaspirillum seropedicae SmR1] Length = 456 Score = 142 bits (357), Expect = 7e-35, Method: Compositional matrix adjust. Identities = 120/412 (29%), Positives = 192/412 (46%), Gaps = 65/412 (15%) Query: 17 RPELVFTEGQGMWLTDHHGKRYLDYLQGWAVNTLGHAPQCIADALAAQSKKLINPSPAFY 76 R + + G+ D G ++D G V+++G + +A+ QS+ L++ S Sbjct: 46 RMPIAMDQAFGVTFKDPDGNTFIDLSAGVGVSSVGRCNPRVVEAIRKQSESLMH-SMEVN 104 Query: 77 NAPSMELA----KLLTDNSVFDRV-FFANSGAEANEGAIKLARKWGKKNPAGDGSARFEI 131 ++ ELA +++ D D + FF G++A E A+K A++ + R +I Sbjct: 105 SSKRTELAAKISEIMPDGLRGDCITFFTQGGSDALEAAVKFAKRV---------TGRHQI 155 Query: 132 ITFKHSFHGRTLATMSASGKDGWDTMFAPQVPG-----------FPKATLNDLAS----- 175 I F +HG A+ + + + F P + G FP T + A Sbjct: 156 IAFHGGYHGIWNASNALTTGTAYRKGFGPFMGGVIHAPYPYAYRFPFDTSHKSAEQIAGE 215 Query: 176 -VEALIGS------HTVAVMLEPVQGEGGVIPASKEFMQGLRRLTKEKNLLLIVDEVQSG 228 V+ L+ + AV++EPVQGEGG +P S EF+Q LR+ LLIVDEVQ+G Sbjct: 216 YVDYLLNTPYTAADDVAAVIVEPVQGEGGYVPPSPEFLQILRKACDRSGALLIVDEVQAG 275 Query: 229 MGRTGQLFAYQHSGIEPDIMTLAKGIGGGVPLAALLAREEIAC-FEAGEQGGTYNGNPLM 287 GRTG+++A +HSG++PD++T KGIGG +P+A L+ R ++A G Q T+ N + Sbjct: 276 AGRTGKMWAVEHSGVKPDMLTFGKGIGGDMPMAGLVMRSDLAAKIPDGSQPNTFAANSIS 335 Query: 288 TAVGVAVIKELLKPGFMETVRERGQYLRQRSLEISEKYGFEGE-RGEGLLRAMQLGRDIG 346 AV + I L P R L + S + GE RG GL+ IG Sbjct: 336 AAVALTNISILQDPRLDLVNRAHTLGLEAQERIRSFNSPWVGEVRGRGLM--------IG 387 Query: 347 PQIVEAARNLEPVG----------------LLLNSPR-PNLLRFMPALNVSK 381 ++VE EP+ L++ R N++R MP+L + + Sbjct: 388 IELVENRETREPLSREKLGKLMDYVVGHGVLMIPCGRYTNVMRVMPSLTIPR 439 >gi|300310456|ref|YP_003774548.1| family II aminotransferase [Herbaspirillum seropedicae SmR1] Length = 458 Score = 129 bits (324), Expect = 4e-31, Method: Compositional matrix adjust. Identities = 118/423 (27%), Positives = 200/423 (47%), Gaps = 49/423 (11%) Query: 20 LVFTEGQGMWLTDHHGKRYLDYLQG-WAVNTLGHAPQCIADALAAQSKKLINPSPAFYNA 78 L+ GQG +LTDH K+ LD G W VNT G+ + + A Q ++L + F+ A Sbjct: 30 LILKSGQGAYLTDHQDKQLLDAFSGLWCVNT-GYGQESVIRAATEQMQRLPYATGYFHFA 88 Query: 79 --PSMELAKLLTDNS--VFDRVFFANSGAEANEGAIKLARKWGKKNPAGDGSARFEIITF 134 P++ LAK L D S V+F G++A + AI+ + + G F I+ Sbjct: 89 SEPAILLAKKLVDISPASLQHVYFTLGGSDAVDAAIRYITHY-HHSIGKPGKQHF--ISL 145 Query: 135 KHSFHGRTLATMSASGKDGWDTMFAPQVPG-----FPKATLNDL--------ASVEAL-- 179 + +HG + + + F +P P ++L ASV+AL Sbjct: 146 QRGYHGSSSVGAGLTALPNFHYHFNLPLPTQHYLPSPYPYRSELQGDEAIIAASVQALRD 205 Query: 180 ----IGSHTVAVML-EPVQGEGGVIPASKEFMQGLRRLTKEKNLLLIVDEVQSGMGRTGQ 234 +G+ VA EP+QG GGV+ K +++ ++ E ++L +VDEV +G GRTG Sbjct: 206 KVAELGADNVAAFFCEPIQGSGGVVVPPKGWLKAMQLACNELDILFVVDEVITGFGRTGP 265 Query: 235 LFAYQHSGIEPDIMTLAKGIGGG-VPLAALLAREEIACFEAG--------EQGGTYNGNP 285 +FA ++PD+MT+AKG+ G P+ A++ + + A G TY+ +P Sbjct: 266 MFACLDEDVQPDLMTMAKGLTAGYAPMGAVMMSDRVYNGIADGAPMSTVVGHGQTYSAHP 325 Query: 286 LMTAVGVAVIKELLKPGFMETVRERGQYLRQRSLEISEKYGFEGE-RGEGLLRAMQLGRD 344 + AV + V++ + G ++ R R + + L + G+ R GLL A++L D Sbjct: 326 VSAAVALEVLRLYEEGGLLDNGR-RLEPVFAGGLRALLAHPLVGDARSRGLLGALELVAD 384 Query: 345 ------IGPQIVEAARNLEPV---GLLLNSPRPNLLRFMPALNVSKEEIDQMFSMLEEVL 395 P++ R + GL+ + N+L F PAL ++E+ MF+ L+ +L Sbjct: 385 KQTKARFAPELKLHDRIFQAAYRHGLVFRAFGDNILGFAPALCYTQEDFALMFARLKAIL 444 Query: 396 AKI 398 ++ Sbjct: 445 DEV 447 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.135 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,195,229 Number of extensions: 50678 Number of successful extensions: 178 Number of sequences better than 1.0e-08: 10 Number of HSP's gapped: 157 Number of HSP's successfully gapped: 11 Length of query: 400 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 313 Effective length of database: 1,207,925 Effective search space: 378080525 Effective search space used: 378080525 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)