BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3285.p (414 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312616|ref|YP_003776708.1| major facilitator superfamily p... 776 0.0 gi|300313879|ref|YP_003777971.1| major facilitator superfamily p... 420 e-118 gi|300310615|ref|YP_003774707.1| major facilitator superfamily p... 96 8e-21 gi|300310449|ref|YP_003774541.1| 4-hydroxybenzoate transporter t... 88 1e-18 gi|300310666|ref|YP_003774758.1| major facilitator superfamily p... 87 2e-18 >gi|300312616|ref|YP_003776708.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 414 Score = 776 bits (2004), Expect = 0.0, Method: Compositional matrix adjust. Identities = 384/409 (93%), Positives = 395/409 (96%) Query: 1 MWLQETTTSERRTLTATFTGYAVDGFDYMIYTFLIPTLLAAWSMSKAEAGYIATGALLTS 60 MWLQETTTSERRTLTATFTGYAVDGFDYMIYTFLIPTLLAAWSMSKAEAGYIATGALLTS Sbjct: 1 MWLQETTTSERRTLTATFTGYAVDGFDYMIYTFLIPTLLAAWSMSKAEAGYIATGALLTS 60 Query: 61 AVGGWAAGVLADRYGRVRVLQWTVLWFALFTFLSGFTNSFGQLFFTRAMQGFGMGGEWAV 120 A+GGWAAGVLADRYGRVRVLQWTVLWFA FTFLSGFT SFGQLFFTRAMQGFGMGGEWAV Sbjct: 61 AIGGWAAGVLADRYGRVRVLQWTVLWFAFFTFLSGFTTSFGQLFFTRAMQGFGMGGEWAV 120 Query: 121 GSVLIAETISARHRGKASGLVQSSWAVGWAASALAFWGIYAVLPPEMAWKVLFWIGILPA 180 GSVLIAETISARHRGKASGLVQSSWAVGWA SALAFWG+YAVLPPE AWKVLFWIG+LPA Sbjct: 121 GSVLIAETISARHRGKASGLVQSSWAVGWAGSALAFWGVYAVLPPETAWKVLFWIGVLPA 180 Query: 181 LLTLYIRRTLHEPEIYLATQARLRQSGQRGNFLRIFHRDLLRTTLLASLLATGMQGAYYS 240 LLTLYIRR + EP +YLATQARLR+SGQRGNFLRIFHRDLLRTTLLASLLATGMQGAYYS Sbjct: 181 LLTLYIRRAISEPAVYLATQARLRESGQRGNFLRIFHRDLLRTTLLASLLATGMQGAYYS 240 Query: 241 VTTWLPTFLKTERHLSVLGTSTYLLVLIGGSFCGYLTSAWLSDTIGRRRCFMFFAVMGIV 300 VTTWLPTFLKTERHLSVLGTSTYLLVLIGGSFCGYLTSAWLSDTIGRRRCFM FA MGIV Sbjct: 241 VTTWLPTFLKTERHLSVLGTSTYLLVLIGGSFCGYLTSAWLSDTIGRRRCFMLFAAMGIV 300 Query: 301 LVLSYTQMVITDAWMLALGFVLGFFLSGIFAGMGAYLSELFPSDVRGSGQGFCYNVGRAL 360 LVLSYTQM+ITDAWMLALGFVLGFFLSGIF+GMGAYLSELFPSDVRGSGQGFCYN GRA+ Sbjct: 301 LVLSYTQMIITDAWMLALGFVLGFFLSGIFSGMGAYLSELFPSDVRGSGQGFCYNFGRAV 360 Query: 361 GAICPALVGYLGNSLPLGQTIGWVAAACYALVILACLALPETRGKELEA 409 GAICPALVGYL NSLPLGQTIGW+AA CY LVI+ACL LPETRGKELEA Sbjct: 361 GAICPALVGYLSNSLPLGQTIGWIAAGCYGLVIVACLVLPETRGKELEA 409 >gi|300313879|ref|YP_003777971.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 413 Score = 420 bits (1079), Expect = e-118, Method: Compositional matrix adjust. Identities = 212/407 (52%), Positives = 284/407 (69%) Query: 2 WLQETTTSERRTLTATFTGYAVDGFDYMIYTFLIPTLLAAWSMSKAEAGYIATGALLTSA 61 W ++ T ER+T A F+G+A+D D +++ IP L+AA+S+SKA+AG I+ L+ SA Sbjct: 4 WYRQVTAKERKTFWACFSGWALDALDVQMFSLAIPALIAAFSLSKADAGLISGATLVASA 63 Query: 62 VGGWAAGVLADRYGRVRVLQWTVLWFALFTFLSGFTNSFGQLFFTRAMQGFGMGGEWAVG 121 +GGW AG L+DRYGRV+ LQ T++WF+ FTFLS F SF QL +A+QGFG GGEWA G Sbjct: 64 IGGWFAGALSDRYGRVKTLQITIIWFSFFTFLSAFAQSFPQLLVLKALQGFGFGGEWAAG 123 Query: 122 SVLIAETISARHRGKASGLVQSSWAVGWAASALAFWGIYAVLPPEMAWKVLFWIGILPAL 181 +VL+AETI A HRGKA G VQS+WAVGW + L F ++++LP EMAW+V+F IG+LPAL Sbjct: 124 AVLMAETIRAEHRGKAMGTVQSAWAVGWGGAVLLFSVLFSLLPQEMAWRVMFVIGLLPAL 183 Query: 182 LTLYIRRTLHEPEIYLATQARLRQSGQRGNFLRIFHRDLLRTTLLASLLATGMQGAYYSV 241 L +Y+RR++ E + +LA Q L IF D+LR T++ SLL G G YY++ Sbjct: 184 LVVYVRRSVQETDAFLAGQKAAASKPAASTLLGIFRPDVLRLTIIGSLLGVGAHGGYYAL 243 Query: 242 TTWLPTFLKTERHLSVLGTSTYLLVLIGGSFCGYLTSAWLSDTIGRRRCFMFFAVMGIVL 301 TWLPT+LKTE+HLSVL T YL V+I +CG + S+ L D IGRRR FFA+ + Sbjct: 244 MTWLPTYLKTEKHLSVLSTGGYLAVIIVAFWCGCIASSQLLDRIGRRRTVAFFALACVAT 303 Query: 302 VLSYTQMVITDAWMLALGFVLGFFLSGIFAGMGAYLSELFPSDVRGSGQGFCYNVGRALG 361 VL Y + ++D ML GF LGFF +GI A MGA +EL+PS +RG+G GFCYN GR Sbjct: 304 VLCYIFLPLSDTQMLVCGFPLGFFAAGIPASMGALFNELYPSGIRGTGVGFCYNFGRVAS 363 Query: 362 AICPALVGYLGNSLPLGQTIGWVAAACYALVILACLALPETRGKELE 408 A P LVG++ +S+PLGQ IG AA Y+LV++A L LPET+G+++E Sbjct: 364 AGFPVLVGHMSDSMPLGQAIGIDAAIAYSLVVVAVLMLPETKGRKME 410 >gi|300310615|ref|YP_003774707.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 478 Score = 95.5 bits (236), Expect = 8e-21, Method: Compositional matrix adjust. Identities = 112/432 (25%), Positives = 174/432 (40%), Gaps = 60/432 (13%) Query: 24 DGFDYMIYTFLIPTLLAAWSMSKAEAGYIATGALLTSAVGGWAAGVLADRYGRVRVLQWT 83 D F + F++P L+ W +S A+ G + L +G G +A+RYGRV T Sbjct: 56 DAFHALSLAFVLPVLVGLWQLSAAQIGLLIASGYLGQVIGALFFGWMAERYGRVPSASVT 115 Query: 84 VLWFALFTFLSGFTNSFGQLFFTRAMQGFGMGGEWAVGSVLIAETISARHRGKASGLVQS 143 V ++ + + T SF LF R +QG G+GGE V + I E A RGK L + Sbjct: 116 VGLMSVLSIVCALTGSFHMLFLCRFLQGIGVGGEVPVAATYINELSQAHGRGKFFVLYEL 175 Query: 144 SWAVGWAASALAFWGIYAVLPPEMAWKVLFWIGILPALLTLYIRRTLHEPEIYLATQARL 203 + +G A+A + A L P W+ +F +G +P +L + L E +L + RL Sbjct: 176 IFPLGLLAAA----QVGAFLVPRFGWEYMFLVGGIPGILVALLITRLPESPRWLIARGRL 231 Query: 204 RQSG--------------------------------------QRGNFLRIFHRDLLRTTL 225 + QRG++ +F T+ Sbjct: 232 DDAEKLIRTLEASTPKRNLDAQTDKQAIEQRINALAAGAGKRQRGSWKELFSPVYRGRTI 291 Query: 226 LASLLATGMQGAYYSVTTWLPTFLKTERHLSVLGTSTYLLVLIGGSFCGYLTSAWLSDTI 285 + LL + WLP+ KT HL + + V S C L A L D + Sbjct: 292 IVWLLWASSYFVANGINNWLPSLYKTVYHLPLQESLRMASVSNVLSACAVLGCALLIDRV 351 Query: 286 GRRRCFMFFAVMGIVLVLSYTQMVITDAWMLALGFVLGFFLSGIFAGMGA-------YLS 338 GRRR + ++ L+ + AW + F S +A MG Y Sbjct: 352 GRRRWAIASFILSGALLALLGVLKAESAWSVM------FLASSAYAVMGTTTVLLYLYTP 405 Query: 339 ELFPSDVRGSGQGFCYNVGRALGAICPALVGYL--GNSLPLGQTIGWVAAACYALVILAC 396 E++P+ +R G G + RA A PA+VG + G+ + T+ + A L +LA Sbjct: 406 EIYPTRMRAIGTGLATSWLRAASAAAPAMVGVVLAGHGI---ATVFLMFAGVTVLGLLAA 462 Query: 397 LALPETRGKELE 408 + ET + LE Sbjct: 463 WRMIETTNRSLE 474 >gi|300310449|ref|YP_003774541.1| 4-hydroxybenzoate transporter transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 447 Score = 87.8 bits (216), Expect = 1e-18, Method: Compositional matrix adjust. Identities = 100/386 (25%), Positives = 154/386 (39%), Gaps = 54/386 (13%) Query: 24 DGFDYMIYTFLIPTLLAAWSMSKAEAGYIATGALLTSAVGGWAAGVLADRYGRVRVLQWT 83 DGFD ++ P ++A W +SKA G + L +G + DRYGR VL + Sbjct: 41 DGFDVQAMGYVAPAIIADWGVSKASLGPVFGAGLFGMLLGSLVLTPVGDRYGRRPVLILS 100 Query: 84 VLWFALFTFLSGFTNSFGQLFFTRAMQGFGMGGEWAVGSVLIAETISARHRGKASGLVQS 143 L+FAL + + QL R + GFG+G L+ E + R LV Sbjct: 101 TLFFALCMLATPMVTTLDQLLVLRFITGFGLGSIMPNAMALVGEFSPSSSRVTRMMLVSC 160 Query: 144 SWAVGWAASALAFWGIYAVLPPEMAWKVLFWI-GILPALLTLYIRRTLHEPEIYLATQAR 202 + VG AA + A L P W +FW+ G +P +L L + L E +L R Sbjct: 161 GFTVGAAAGGF----VSAALIPAYGWHAVFWVGGAVPLVLGLAMLVWLPESIQFLVLHRR 216 Query: 203 LRQSGQRGNFLRIFHRDLL---RTTL-----------LASLLATGMQGA----------- 237 R Q +LR +++ +T + +A+L G G Sbjct: 217 PRT--QVARWLRKLDPNIVIDDKTEVVVKETKAEGMPVAALFRDGRAGVTVLLWLISFMN 274 Query: 238 ---YYSVTTWLPTFLKTERHLSVLGTSTYLLVLIG-----GSFCGYLTSAWLSDTIGRRR 289 Y ++ WLPT ++ G ST + VLIG G G L+ G R Sbjct: 275 LINLYFLSNWLPTLIRDA------GYSTSMAVLIGTSLQVGGVIGTLSLGRFIQRFGFTR 328 Query: 290 ----CFMFFAVMGIVLVLSYTQMVITDAWMLALGFVLGFFLSGIFAGMGAYLSELFPSDV 345 CF+ A + I L+ ++ A + V GF + G + A +P+ + Sbjct: 329 VLGSCFL-LACLSIALI---GKLATVPALLFVAVIVAGFCIVGGQPAVNALAGTFYPTTL 384 Query: 346 RGSGQGFCYNVGRALGAICPALVGYL 371 R +G G+ +GR + P + G L Sbjct: 385 RSTGIGWALGIGRIGSVVGPVIGGQL 410 >gi|300310666|ref|YP_003774758.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 438 Score = 87.4 bits (215), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 108/412 (26%), Positives = 183/412 (44%), Gaps = 35/412 (8%) Query: 24 DGFDYMIYTFLIPTLLAAWSMSKAEAGYIATGALLTSAVGGWAAGVLADRYGRVRVLQWT 83 D + F PT+ + ++ +A T + +G + G LADR GR VL Sbjct: 35 DAMQVLSIGFSAPTIAKTFGLTVPQALQTGTMFFVGMLLGAFVFGRLADRIGRRPVLMGA 94 Query: 84 VLWFALFTFLSGFTNSFGQLFFTRAMQGFGMGGEWAVGSVLIAETISARHRGKASGLVQS 143 V+ A F S F F L R + G G+GG V ++AE + + RG+ +++S Sbjct: 95 VVIDACFGVASAFAPDFTWLLALRLLTGIGVGGTLPVDYTMMAEFLPSARRGRWLVMLES 154 Query: 144 SWAVGWAASALAFWGIYAVLPPEMAWKVLFWIGILPALLTLYIRRTLHEPEIYL------ 197 WA+G LA + AV AW+V+F++ LPAL+ + +R + E +YL Sbjct: 155 FWAIG--TICLAVLALVAVSWGNDAWRVIFFVTGLPALVGVVLRFYVPESPMYLNRSGKS 212 Query: 198 --ATQARLRQSGQRGNFLRI--FHRDLLRTTLLASLLATGMQG--------------AYY 239 A Q R + G+ I +++ +++L + ++ AYY Sbjct: 213 DAARQVLERVAKVNGSSTPIPPLQPEVVERKPMSALFSAALRRRSLSLYLAWGLISIAYY 272 Query: 240 SVTTWLPTFLKTERHLSVLGTSTYLLVLIGGSFCGYLTSAWLSDTIGRRRCFMFFAVMGI 299 V +LP L +E + +L+ L GY SA+ + GR+ + F V+ Sbjct: 273 GVFVYLPVKLGSE-GFGFMRGQIFLIFLALVQLPGYALSAYGVERWGRKPTLVGFLVLSA 331 Query: 300 VLVLSYTQMVITDAWMLALGFVLGFFLSGIFAGMGAYLSELFPSDVRGSGQGFCYNVGRA 359 V + Y+ + ++ +L F L G +A + A+ E++P+D+R SG G V R Sbjct: 332 VGCMLYS-LGKDPMLVVGSTLLLSFSLLGTWAALYAFTPEVYPTDLRASGMGTAGAVARF 390 Query: 360 LGAICPALVGYLGNS---LPLGQTIGWVAAACYALVILACLALPETRGKELE 408 G PA+V + S L L ++ A A++ C+ + E+R + LE Sbjct: 391 GGLFAPAIVAPVMASHFTLALAMLSAFLVAGAVAIL---CVDV-ESRNRALE 438 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.327 0.139 0.445 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,363,396 Number of extensions: 61742 Number of successful extensions: 412 Number of sequences better than 1.0e-08: 25 Number of HSP's gapped: 360 Number of HSP's successfully gapped: 26 Length of query: 414 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 326 Effective length of database: 1,203,190 Effective search space: 392239940 Effective search space used: 392239940 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)