BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3244.p (413 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312582|ref|YP_003776674.1| O-acetylhomoserine sulfhydrylas... 817 0.0 gi|300312963|ref|YP_003777055.1| o-acetylhomoserine sulfhydrylas... 214 7e-57 gi|300312294|ref|YP_003776386.1| cystathionine beta-lyase [Herba... 117 2e-27 >gi|300312582|ref|YP_003776674.1| O-acetylhomoserine sulfhydrylase [Herbaspirillum seropedicae SmR1] Length = 413 Score = 817 bits (2111), Expect = 0.0, Method: Compositional matrix adjust. Identities = 392/413 (94%), Positives = 400/413 (96%) Query: 1 MNDKKTYGFTTTILHSDRQKGIEHGSLHKPIHTSVTFGYEDARQLAEVFQGKQPGYRYGR 60 MNDKKTYGFTTTILHSDRQKGIEHGSLHKPIHTSVTFGYEDARQLAEVFQGKQPGYRYGR Sbjct: 1 MNDKKTYGFTTTILHSDRQKGIEHGSLHKPIHTSVTFGYEDARQLAEVFQGKQPGYRYGR 60 Query: 61 QGNPTVSALEDKITKMEDGKSTICFATGMAAIGAIVQGLLREGDHVVSSAFLFGNTNSLW 120 QGNPTV+ALEDKITKMEDGKSTICFATGMAAIGAIVQGLLREGDHVVSSAFLFGNTNSLW Sbjct: 61 QGNPTVAALEDKITKMEDGKSTICFATGMAAIGAIVQGLLREGDHVVSSAFLFGNTNSLW 120 Query: 121 MTVGAQGAKVSMVDATDVKNVEAAITPNTRLVFVETIANPRTQVADLKRIGELCRQRGIL 180 MTVGAQGAKVSMVDATDVKNVEAAIT NTRLVFVETIANPRTQVADLKRIGELCR+RGIL Sbjct: 121 MTVGAQGAKVSMVDATDVKNVEAAITANTRLVFVETIANPRTQVADLKRIGELCRERGIL 180 Query: 181 YVVDNTMTSPYLFRPKTVGAGLVVNSLTKSIGGHGNALGGALTDTGEYDWTRYPHIADNY 240 YVVDNTMTSPYLFRPKTVGAGLVVNSLTKSIGGHGNALGGALTDTGE+DWTRYPHIA+NY Sbjct: 181 YVVDNTMTSPYLFRPKTVGAGLVVNSLTKSIGGHGNALGGALTDTGEFDWTRYPHIAENY 240 Query: 241 KKNPPAQWAMAQIRAKALRDFGGSLGPEAAHHIAVGAETIALRLDRECQNALALAQMLQA 300 KKNP QW MAQIRAKALRDFGGSLGPEAAHHIAVGAETIALR +REC+NALALAQMLQA Sbjct: 241 KKNPAPQWGMAQIRAKALRDFGGSLGPEAAHHIAVGAETIALRQERECKNALALAQMLQA 300 Query: 301 DPRVAAVHYPGLESHPQHALCKQLFRSFGSLMSFELKDSIDCFDYLNRLNLAVAASNLGD 360 D RVAAV+YPGLESHPQHAL K LFRSFGSLMSFELKD IDCFDYLNRL LA+ SNLGD Sbjct: 301 DERVAAVYYPGLESHPQHALSKALFRSFGSLMSFELKDGIDCFDYLNRLRLAIPTSNLGD 360 Query: 361 TRTLVIPVAHTIFYEMGAERRASMGIAESLIRVSVGLEDTDDLVQDFRQALDA 413 TRTLVIPVAHTIFYEMGAERRASMGIAESLIRVSVGLEDTDDLV DFRQALDA Sbjct: 361 TRTLVIPVAHTIFYEMGAERRASMGIAESLIRVSVGLEDTDDLVADFRQALDA 413 >gi|300312963|ref|YP_003777055.1| o-acetylhomoserine sulfhydrylase [Herbaspirillum seropedicae SmR1] Length = 429 Score = 214 bits (546), Expect = 7e-57, Method: Compositional matrix adjust. Identities = 136/428 (31%), Positives = 224/428 (52%), Gaps = 15/428 (3%) Query: 1 MNDKKTYGFTTTILHSDRQKGIEHGSLHKPIHTSVTFGYEDARQLAEVFQGKQPGYRYGR 60 M D + F T +H ++ PI+ +V F ++D + A++F K G Y R Sbjct: 1 MPDNPQWKFETISVHGGYDPDPTTRAVAVPIYQTVAFAFDDTQHGADLFDLKVQGNIYSR 60 Query: 61 QGNPTVSALEDKITKMEDGKSTICFATGMAAIGAIVQGLLREGDHVVSSAFLFGNTNSLW 120 NPT LE ++ +E G + A+G AA+ +Q + GD++VS++ L+G T +L+ Sbjct: 61 IMNPTQDVLEKRLAALEGGIGALALASGQAAVTYAIQTIAEAGDNIVSASTLYGGTYNLF 120 Query: 121 M-TVGAQGAKVSMVDATDVKNVEAAITPNTRLVFVETIANPRTQVADLKRIGELCRQRGI 179 T+ G + D + E I T+ +++E+I NP + D+ + ++ + G+ Sbjct: 121 AHTLPQYGVQTRFADPRQPASFEPLIDDRTKAIYIESIGNPLGNITDIAAVADIAHRHGV 180 Query: 180 LYVVDNTMTSPYLFRPKTVGAGLVVNSLTKSIGGHGNALGGALTDTGEYDWTRY------ 233 +VDNT+ +PYL RP GA +VV+SLTK +GGHG LGGA+ D+G++ W ++ Sbjct: 181 PLIVDNTVATPYLLRPFEHGADIVVHSLTKYLGGHGTTLGGAIVDSGKFPWAKHKQRFKR 240 Query: 234 ---PHIADN---YKKNPPAQWAMAQIRAKALRDFGGSLGPEAAHHIAVGAETIALRLDRE 287 P ++ + Y + + + R LR+ G +L P A I G ET+ LRLDR Sbjct: 241 LNEPDVSYHGVVYTEALGEAAYIGRARVVPLRNMGAALSPFNAFQILQGIETLGLRLDRI 300 Query: 288 CQNALALAQMLQADPRVAAVHYPGLESHPQHALCKQLFRSFGS-LMSFELKDSID-CFDY 345 N LA+AQ L+ P+V V+Y GLE HP HAL + F+ S +++F + + + + Sbjct: 301 TANTLAVAQYLKRHPKVRWVNYAGLEDHPDHALAQTYFKGRASGVLTFGVANGREGGARF 360 Query: 346 LNRLNLAVAASNLGDTRTLVIPVAHTIFYEMGAERRASMGIAESLIRVSVGLEDTDDLVQ 405 + L L N+GD ++L A T ++ G+ E +R+S+G+E DDL+ Sbjct: 361 QDALQLFTRLVNIGDAKSLATHPASTTHRQLSPTELEKAGVTEDTVRLSIGIEHIDDLLA 420 Query: 406 DFRQALDA 413 D QAL + Sbjct: 421 DLEQALQS 428 >gi|300312294|ref|YP_003776386.1| cystathionine beta-lyase [Herbaspirillum seropedicae SmR1] Length = 392 Score = 117 bits (293), Expect = 2e-27, Method: Compositional matrix adjust. Identities = 107/418 (25%), Positives = 180/418 (43%), Gaps = 36/418 (8%) Query: 1 MNDKKTYGFT--TTILHSDRQKGIEHGSLHKPIHTSVTFGYEDARQLAEVFQGKQPGYRY 58 M+D K T T+++HS + + IH + T + D + ++ GY Y Sbjct: 1 MSDPKQTSLTPQTSLVHSSYRAPDGFEAFPVGIHHASTVLFPDVAAMRSRDWREKLGYTY 60 Query: 59 GRQGNPTVSALEDKITKMEDGKSTICFATGMAAIGAIVQGLLREGDHVVSSAFLFGNTNS 118 G G PT LE ++ ++E GK +G+AAI + L+ GD V+ ++G + Sbjct: 61 GLHGTPTSFVLEARLAEIEGGKYCRVTPSGLAAIAMVNFAFLKSGDDVLLPDNVYGPSKD 120 Query: 119 L--WMTVGAQGAKVSMVDATDVKNVEAAITPNTRLVFVETIANPRTQVADLKRIGELCRQ 176 L W+ Q D + + I P TRLV+ E + +V D+ + Sbjct: 121 LGQWLERDFQ-ITARFYDPMIGEGLAELIRPTTRLVWTEAPGSVSMEVPDIPALCRAAHA 179 Query: 177 RGILYVVDNTMTSPYLFRPKTVGAGLVVNSLTKSIGGHGNALGGALTDTGEYDWTRYPHI 236 +G+L +DNT ++ F+ G +V+ ++TK G + L GA+ I Sbjct: 180 KGVLVAIDNTWSAGIAFKAFEHGVDIVMQAVTKYQSGGSDVLMGAV-------------I 226 Query: 237 ADNYKKNPPAQWAMAQIRAKALRDFGGSLGPEAAHHIAVGAETIALRLDRECQNALALAQ 296 + N + +A +R G +G + + + ++ LR + A +A Sbjct: 227 VRDQALNEKLE--LAHMR------LGFGVGMDDVYMVLRSLSSMRLRFEAHDAAARKVAA 278 Query: 297 MLQADPRVAAVHYPGLESHPQHALCKQLFRSFGSLMS--FELKDSIDCFD-YLNRLNLAV 353 L+ P + AV +P E P HA K+ F G L S F+ + D +++ L L Sbjct: 279 WLKQRPEIVAVLHPAFEDCPGHAHWKRDFSGAGGLFSVIFDPRYVEAQTDRFVDSLRLFK 338 Query: 354 AASNLGDTRTLVIPVAHTIFYEMGAERRASMGIAESLIRVSVGLEDTDDLVQDFRQAL 411 + G +L +P Y M RRA M + L+R ++GLE +DL+ D QAL Sbjct: 339 IGYSWGGAHSLCVP------YRMKGMRRAWMREGQ-LVRFNIGLESPEDLIADIEQAL 389 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.134 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,277,291 Number of extensions: 52684 Number of successful extensions: 140 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 136 Number of HSP's successfully gapped: 3 Length of query: 413 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 325 Effective length of database: 1,203,190 Effective search space: 391036750 Effective search space used: 391036750 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)