BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3227.p (523 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312566|ref|YP_003776658.1| multidrug resistance protein pr... 962 0.0 gi|300312017|ref|YP_003776109.1| multidrug resistance B (translo... 417 e-117 gi|300312486|ref|YP_003776578.1| major facilitator superfamily p... 205 9e-54 gi|300312683|ref|YP_003776775.1| major facilitator superfamily p... 121 1e-28 gi|300310856|ref|YP_003774948.1| major facilitator superfamily p... 120 2e-28 >gi|300312566|ref|YP_003776658.1| multidrug resistance protein protein [Herbaspirillum seropedicae SmR1] Length = 525 Score = 962 bits (2486), Expect = 0.0, Method: Compositional matrix adjust. Identities = 474/500 (94%), Positives = 490/500 (98%) Query: 24 LSGGQLVGAGILLAAANFIAVLDTTIANVSVSTISGALGASTSQGTYVITSYAVAEAITV 83 LSGGQLVGAGILLAAANFIAVLDTTIANVSVSTI+GALGASTSQGTYVITSYAVAEAITV Sbjct: 26 LSGGQLVGAGILLAAANFIAVLDTTIANVSVSTIAGALGASTSQGTYVITSYAVAEAITV 85 Query: 84 PLTGWLANRFGTVRVFILSMIMFGLFSALCGLANSLGMLIAFRVMQGLAGGPLMPLSQTL 143 PLTGWLANRFGTVRVFILSM+MFGLFSALCG+ANSLGMLIAFRV+QGLAGGPLMPLSQTL Sbjct: 86 PLTGWLANRFGTVRVFILSMVMFGLFSALCGMANSLGMLIAFRVLQGLAGGPLMPLSQTL 145 Query: 144 LLRIFPKEKAPAAVGLWAMTTLVAPIAGPILGGVLCDQYSWPYIFWINVPVALICGYLGW 203 LLRIFPKEKAPAAVGLWAMTTLVAPIAGPILGGVLCDQYSWPYIFWINVPVALICGY+GW Sbjct: 146 LLRIFPKEKAPAAVGLWAMTTLVAPIAGPILGGVLCDQYSWPYIFWINVPVALICGYIGW 205 Query: 204 RMLKRFETKTSKLPIDRIGLILMVVWVAALQLMLDEGKEKDWFASTEIVLLAITAVVFFI 263 RMLKR+ETK SKLPIDR+GL+LMVVWVAALQLMLDEGKEKDWFASTEIVLLA+TAVVFFI Sbjct: 206 RMLKRYETKMSKLPIDRVGLVLMVVWVAALQLMLDEGKEKDWFASTEIVLLAVTAVVFFI 265 Query: 264 AFLIWELTEHNPIVDLRVFRHRGFTASVTTICLAFGAFFGSVVLTPLWLQGYMGYTATWS 323 AFLIWELTE NPIVDLRVFRHRGFTASVTTICLAFGAFFGSVVLTPLWLQGYMGYTATWS Sbjct: 266 AFLIWELTERNPIVDLRVFRHRGFTASVTTICLAFGAFFGSVVLTPLWLQGYMGYTATWS 325 Query: 324 GCISALTGILAVMTAPLVAKMSATIDARKLVCFGVTWLGLITFVRAFNTTDMSFFQIGWP 383 GCISALTGILAVMTAP+VAKMS TIDARKLVC GVTWLG ITF+RAFNTTDMSFFQIGWP Sbjct: 326 GCISALTGILAVMTAPMVAKMSTTIDARKLVCIGVTWLGFITFLRAFNTTDMSFFQIGWP 385 Query: 384 LFLQGIGLPLFFVPLTSLALSSVNEEETASAAGLMSFCRTMSGAIATSIVNTSWENDATH 443 LFLQGIGLPLFFVPLT LALSSVNEEETASAAGLMSFCRTM+GAIATSIVNTSWENDAT+ Sbjct: 386 LFLQGIGLPLFFVPLTGLALSSVNEEETASAAGLMSFCRTMAGAIATSIVNTSWENDATY 445 Query: 444 FHAELAGLVDRSGAAIQSMMASGMSLEQATASLAQTVQNQAVMLSTNHIFMMAAVSFSLA 503 FHAEL+GLVDR G AI +MMASGMS EQATASLAQTVQNQAVMLSTNHIFMMAAVSFSLA Sbjct: 446 FHAELSGLVDRGGDAIHTMMASGMSAEQATASLAQTVQNQAVMLSTNHIFMMAAVSFSLA 505 Query: 504 ALAVWLAPKPTRVADTTAAH 523 ALAVWLAPKPTRVADT+AAH Sbjct: 506 ALAVWLAPKPTRVADTSAAH 525 >gi|300312017|ref|YP_003776109.1| multidrug resistance B (translocase) transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 524 Score = 417 bits (1072), Expect = e-117, Method: Compositional matrix adjust. Identities = 223/497 (44%), Positives = 311/497 (62%), Gaps = 10/497 (2%) Query: 14 APQPGPLEA-----PLSGGQLVGAGILLAAANFIAVLDTTIANVSVSTISGALGASTSQG 68 A P P +A PL+G LV + ++ A F+ VLD++IANV++ TISG LG S +G Sbjct: 2 ATSPTPSQAYTPPPPLTGANLVLGTLSVSLAVFMNVLDSSIANVAIPTISGNLGVSVDEG 61 Query: 69 TYVITSYAVAEAITVPLTGWLANRFGTVRVFILSMIMFGLFSALCGLANSLGMLIAFRVM 128 T+VITS+A A AI++PLTGWL R G VR+F+ S+++F L S LCGLA +L +L+A RV+ Sbjct: 62 TWVITSFAAANAISIPLTGWLTQRLGAVRLFVTSVLLFVLASWLCGLAPTLPILLAARVL 121 Query: 129 QGLAGGPLMPLSQTLLLRIFPKEKAPAAVGLWAMTTLVAPIAGPILGGVLCDQYSWPYIF 188 QGL GP++PLSQ+LLL +PK K+ A+ LW MT +VAP+AGP LGG + D Y+WP+IF Sbjct: 122 QGLVAGPMVPLSQSLLLGAYPKSKSSFALALWGMTAVVAPVAGPALGGWITDSYTWPWIF 181 Query: 189 WINVPVALICGYLGWRMLKRFETKTSKLPIDRIGLILMVVWVAALQLMLDEGKEKDWFAS 248 +IN+PV LI + + + ET T KLPID+IGL L+V WVAA Q+MLD+GK+ DWFAS Sbjct: 182 YINIPVGLIAAGVTLAIYRSRETPTHKLPIDKIGLALLVTWVAAFQIMLDKGKDLDWFAS 241 Query: 249 TEIVLLAITAVVFFIAFLIWELTEHNPIVDLRVFRHRGFTASVTTICLAFGAFFGSVVLT 308 IV L I A++ F F++WEL +P+VDLR+F R F I + +G FFG++V+ Sbjct: 242 PTIVTLGIIALIAFAYFVVWELNNDHPVVDLRLFGRRNFAGGTIAISIGYGVFFGNLVIL 301 Query: 309 PLWLQGYMGYTATWSGCISALTGILAVMTAPLVAKMSATIDARKLVCFGVTWLGLITFVR 368 P WLQ Y+GY + SG +A GI AV+ PL+ + +DARK+ ++ ++R Sbjct: 302 PQWLQQYLGYRSIDSGLSTAPIGIFAVILMPLIGRFLPLVDARKVATASFIGFAIVFWLR 361 Query: 369 AFNTTDMSFFQIGWPLFLQGIGLPLFFVPLTSLALSSVNEEETASAAGLMSFCRTMSGAI 428 + + + P LQGI +FFVPLT L LS + E +AAGL SF R GA+ Sbjct: 362 SRYNLQVDQMTVILPTLLQGIPTAMFFVPLTVLLLSGLPPERIPAAAGLSSFVRVFCGAV 421 Query: 429 ATSIVNTSWENDATHFHAELAGLVDRSGAAIQSMMAS-----GMSLEQATASLAQTVQNQ 483 TSI T+W N + HA+L +Q + + G++ EQ L +T+ Q Sbjct: 422 GTSISTTAWNNRSIMHHAQLTEHATPYSPWLQETVNNLSSTLGLNAEQTAGMLERTITFQ 481 Query: 484 AVMLSTNHIFMMAAVSF 500 A ML N IF ++AV F Sbjct: 482 AAMLGINDIFYVSAVIF 498 >gi|300312486|ref|YP_003776578.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 514 Score = 205 bits (521), Expect = 9e-54, Method: Compositional matrix adjust. Identities = 148/493 (30%), Positives = 250/493 (50%), Gaps = 15/493 (3%) Query: 41 FIAVLDTTIANVSVSTISGALGASTSQGTYVITSYAVAEAITVPLTGWLANRFGTVRVFI 100 +A LD +I N S+ TI G +GAS ++GT++ T+Y VAE + +P++ WL G + + Sbjct: 27 LMATLDISIVNSSLPTIQGEIGASGTEGTWIATAYLVAEIVIIPMSAWLERLLGLRVLLL 86 Query: 101 LSMIMFGLFSALCGLANSLGMLIAFRVMQGLAGGPLMPLSQTLLLRIFPKEKAPAAVGLW 160 + +F LFS LCGL+ +L M+I RV QG GG L+P + T++ P+ + P ++ Sbjct: 87 TAASLFTLFSVLCGLSTTLPMMIVGRVGQGFTGGALIPTAMTIIATRLPRSQQPVGNAMF 146 Query: 161 AMTTLVAPIAGPILGGVLCDQYSWPYIFWINVPVALICGYLGWRMLKRFETKTSKL-PID 219 T ++ P+ GP++GG L + SW Y F+IN+PV L + K +L D Sbjct: 147 GATAILGPVLGPVMGGWLTENVSWHYAFFINLPVGAALITLLLVAIPYERPKFEQLAEAD 206 Query: 220 RIGLILMVVWVAALQLMLDEGKEKDWFASTEIVLLAITAVVFFIAFLIWELTEHNPIVDL 279 G+ + + + L ++L+EG+ + WF+S +I LA T+V+ FI I ++ +P++ L Sbjct: 207 WFGIAGLALGLGGLTVVLEEGEREQWFSSPDIRWLAATSVLGFILLAIGQVRARHPVIQL 266 Query: 280 RVFRHRGFTASVTTICLAFGAFFGSVVLTPLWLQGYMGYTATWSGCISALTGILAVMTAP 339 R+ R R F + V + A +G+ + P +L G GY + SG I L+G+ ++ P Sbjct: 267 RLLRDRQFGSVVMMAMIVGVALYGTAYVIPQFLSGIAGYNSLQSGWIVLLSGVPMILMMP 326 Query: 340 LVAKMSATIDARKLVCFGVTWLGLITFVRAFNTTDMSFFQIGWPL----FLQGIGLPLFF 395 + +D R V G L+ AF TD+S G ++G+G L Sbjct: 327 FTPLLMRLVDIRIAVGTGF----LLLAASAFIETDLSPLSYGGSFVASQLMRGVGTVLAM 382 Query: 396 VPLTSLALSSVNEEETASAAGLMSFCRTMSGAIATSIVNTSWENDATHFHAELAGLVDRS 455 + L A+ SV + A+GL S R + G++A + + + L ++ + Sbjct: 383 LFLNQAAIRSVPPSQAGDASGLYSAARNLGGSLALAAIAVIQDQRLWLHSRRLEESLNAN 442 Query: 456 GAAIQ-SMMASGMSL---EQATASLAQTVQNQAVMLSTNHIFMMAAVS-FSLAALAVWLA 510 +Q SM A G L + A + T+Q+QA+ ++ +F M V+ + L ++L Sbjct: 443 SDLVQSSMTAQGHLLGGQDVALQLMGATIQSQALTMTYADLFWMLGVAILCVTPLVLFLR 502 Query: 511 PKPTRVADTTAAH 523 P PT A A+H Sbjct: 503 PLPTHAAP-VASH 514 >gi|300312683|ref|YP_003776775.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 498 Score = 121 bits (304), Expect = 1e-28, Method: Compositional matrix adjust. Identities = 110/414 (26%), Positives = 188/414 (45%), Gaps = 29/414 (7%) Query: 35 LLAAANFIAVLDTTIANVSVSTISGALGASTSQGTYVITSYAVAEAITVPLTGWLANRFG 94 ++A+ F+ LDTTI N ++ ++ +LG V+ +Y + A P +GWLA+RFG Sbjct: 57 IVASGFFMQTLDTTIVNTALPAMARSLGEPVLNMHPVVVAYTLTMASLTPASGWLADRFG 116 Query: 95 TVRVFILSMIMFGLFSALCGLANSLGMLIAFRVMQGLAGGPLMPLSQTLLLRIFPKEKAP 154 T R++ ++++F L S LC +A+SL ML+A RV+QGL G L+P+ + +LR P + Sbjct: 117 TRRMYFAAILLFSLGSLLCAMASSLPMLLAARVVQGLGGSMLLPVGRLAVLRAVPGAQYI 176 Query: 155 AAVGLWAMTTLVAPIAGPILGGVLCDQYSWPYIFWINVPVALICGYLGWRMLKRF--ETK 212 AA+ ++ + P+ GP+LGG L + SW +IF IN+PV G LG + F + Sbjct: 177 AALASISIAGQLGPVVGPVLGGWLVESLSWHWIFLINLPV----GVLGMGAVLYFLPDDS 232 Query: 213 TSKLP-IDRIGLILMVVWVAALQLMLD---EGKEKDWFASTEIVLLAITAVVFFIAFLIW 268 + P D +G +L+ + A L LD + W I L + A+ + + Sbjct: 233 LAGTPRFDLLGCVLLSACMVAFSLALDVPFDSHRTGW----SIALFVVAALT-VLGYWRH 287 Query: 269 ELTEHNPIVDLRVFRHRGFTASVTTICLAFGAFFGSVVLTPLWLQGYMGYTATWSGCISA 328 P+ L +F F+ + + L PL LQ +GY+ +G + Sbjct: 288 ASVHPRPLFRLGLFGEPNFSIGLAGNLVCRIGSSAVPFLLPLLLQVRLGYSPLHAGMVLL 347 Query: 329 LTGILAVMTAPLVAKMSATIDARKLVCFGVTWLGLITFVRAFNTTDMSFFQIGWPLFLQG 388 I + P +A + + + +G A T D WPL Sbjct: 348 PAAIAGTIAKPWIAPLVKRFGYDRFLLVNTAIVGAAIMSFALITPD-------WPLTAAI 400 Query: 389 IGLPLF-------FVPLTSLALSSVNEEETASAAGLMSFCRTMSGAIATSIVNT 435 + L +F F + S+ L +++ + S L S + ++ + +I T Sbjct: 401 VQLAIFGASNSMQFAAMNSVTLKGLDKRDAGSGNSLFSMVQMLAIGLGVTIGGT 454 >gi|300310856|ref|YP_003774948.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 446 Score = 120 bits (302), Expect = 2e-28, Method: Compositional matrix adjust. Identities = 84/317 (26%), Positives = 158/317 (49%), Gaps = 16/317 (5%) Query: 45 LDTTIANVSVSTISGALGASTSQGTYVITSYAVAEAITVPLTGWLANRFGTVRVFILSMI 104 +D+T+ + S+ ++ + +TSY V+ A+ +P++GW+A+RFG++ +F ++ Sbjct: 1 MDSTVISTSLPALALDMNVDPISLKLALTSYLVSLAVFIPISGWMADRFGSLTIFRSAIC 60 Query: 105 MFGLFSALCGLANSLGMLIAFRVMQGLAGGPLMPLSQTLLLRIFPKEKAPAAVGLWAMTT 164 +F L S LCG++++L +A R +QG+ G ++P+ + ++LR K A+ + Sbjct: 61 VFMLGSILCGVSSTLPQFVAARFLQGIGGAMMVPVGRLVILRSVEKVNLVKALSYLTIPA 120 Query: 165 LVAPIAGPILGGVLCDQYSWPYIFWINVPVALICGYLGWRMLKRFETKTSKLPIDRIGLI 224 L P+ GP LGG + + W +IF+IN+P++++ YL R + + + P+D G + Sbjct: 121 LFGPVLGPPLGGFITTYFHWRWIFFINIPISILGLYLATRFIPDLKAEQVA-PLDLKGFL 179 Query: 225 LMVVWVAALQLMLDEGKEKDWFASTEIVLLAITAVVFFIAFLIWELTEHN-PIVDLRVFR 283 L + + L + L S E+ L + A + F+A +W + P++DL + + Sbjct: 180 LSALGCSLLMMGLATAGRH--LVSGEVALTCVLAGLVFLAAYVWHARRASAPLIDLALLK 237 Query: 284 HRGFTASVTTICLAFGAFFGSV------VLTPLWLQGYMGYTATWSGCISALTGILAVMT 337 A V FG F V L PL LQ GYT SG ++ + A+ Sbjct: 238 IPTLRAGV------FGGSFYRVGIGAIPFLLPLMLQLGFGYTPFHSGMLTCASAAGAMFM 291 Query: 338 APLVAKMSATIDARKLV 354 + A+ R+++ Sbjct: 292 KTIAARFLRRYGFRRVL 308 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.325 0.135 0.414 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,591,941 Number of extensions: 65859 Number of successful extensions: 430 Number of sequences better than 1.0e-08: 21 Number of HSP's gapped: 410 Number of HSP's successfully gapped: 21 Length of query: 523 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 433 Effective length of database: 1,193,720 Effective search space: 516880760 Effective search space used: 516880760 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 133 (55.8 bits)