BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3225.p (515 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312564|ref|YP_003776656.1| outer membrane multidrug resist... 853 0.0 gi|300312015|ref|YP_003776107.1| NodT family protein [Herbaspiri... 275 4e-75 gi|300311423|ref|YP_003775515.1| NodT family protein [Herbaspiri... 245 7e-66 gi|300311155|ref|YP_003775247.1| outer membrane drug efflux lipo... 171 1e-43 gi|300313898|ref|YP_003777990.1| outer membrane drug efflux lipo... 164 2e-41 >gi|300312564|ref|YP_003776656.1| outer membrane multidrug resistance lipoprotein [Herbaspirillum seropedicae SmR1] Length = 515 Score = 853 bits (2205), Expect = 0.0, Method: Compositional matrix adjust. Identities = 445/515 (86%), Positives = 465/515 (90%) Query: 1 MNNTSSPQDPAAPLAQKRGRPWRQTASASAAVLAAALMAGCASFTDLGPRAEPKSIDHYQ 60 MN+ SS D Q+ GR W + S AAVLAA LMAGCASF+DLG RA+PKSID YQ Sbjct: 1 MNSNSSTLDLPIQPPQRSGRAWPRAVSVLAAVLAATLMAGCASFSDLGERAQPKSIDRYQ 60 Query: 61 SSQSLAASALQATWPSDQWWTVYGDAQLNSLIDEALQSAPSMAVAKARLMKAEGAAQQQG 120 S QSLAASA+QA WPSDQWW VYGDAQLN+LIDEALQSAPSMAVAKARLMKAEGAAQQQG Sbjct: 61 SQQSLAASAVQAAWPSDQWWRVYGDAQLNALIDEALQSAPSMAVAKARLMKAEGAAQQQG 120 Query: 121 AALLPEVSGNASLDHMKQSYNNGFPPALVPHGYNNETRTSLDFSYEIDFWGKNRAALAAA 180 AAL P+VS NASLD MKQSYNNG PP VP YNN TR +LDFSYEIDFWGKNRAALAAA Sbjct: 121 AALYPQVSANASLDRMKQSYNNGVPPDFVPKDYNNATRATLDFSYEIDFWGKNRAALAAA 180 Query: 181 TSELEASRADAAQARITLATSIASAYAELAQLYAQRDTNEAALKVRVETLDLFKQRFANG 240 TSELEASRADAAQARITLATSIASAYAELAQLYAQRDTNEAALKVRVE+LDLF QRF NG Sbjct: 181 TSELEASRADAAQARITLATSIASAYAELAQLYAQRDTNEAALKVRVESLDLFNQRFTNG 240 Query: 241 LETRGSVMQMEARRAIAQADLKATDESIALQRNKLAALAGAGPDRGLQLARPQIDLSKPF 300 LETRGSV QMEARRAIAQADLKATDESI LQRNKLAAL GAGPDRGLQL RPQIDLS+PF Sbjct: 241 LETRGSVKQMEARRAIAQADLKATDESIGLQRNKLAALLGAGPDRGLQLTRPQIDLSRPF 300 Query: 301 GLPQQLPVELLGRRPDIVAARLRAEAAGKQIKVARAAFYPNVNLSAYIGFQSLGLSMLTR 360 LP QLPVELLGRRPDIVAARLRAEAAGKQIKVARAAFYPNVNL+AY GFQSLG+ MLTR Sbjct: 301 ALPAQLPVELLGRRPDIVAARLRAEAAGKQIKVARAAFYPNVNLTAYFGFQSLGIDMLTR 360 Query: 361 SGSDIGSVGPAISLPIFNGGRLRGQFRSASASYDEAVANYDQAVTQALQDVADVGVSEKA 420 +GSDIGS+GPAISLPIFNGGRLRGQFRSASASYDEAVANYDQAVTQALQDVADVGVSEKA Sbjct: 361 AGSDIGSIGPAISLPIFNGGRLRGQFRSASASYDEAVANYDQAVTQALQDVADVGVSEKA 420 Query: 421 LTGRLADVKAAADAADEAYRIVSNRYNGGLANYLDVLNAQDTLISNLRQLSDLRSRMFTL 480 L GRLADV+AAADAA+EAYRIVSNRYNGGLA YLDVLNAQDTLISNLRQLSDLRSRMFTL Sbjct: 421 LAGRLADVQAAADAAEEAYRIVSNRYNGGLATYLDVLNAQDTLISNLRQLSDLRSRMFTL 480 Query: 481 DVALVRALGGGYRAADNSKSQDSAHALHTEPQAKG 515 DVALVRALGGGYRAAD+S SQ+SA A + QAKG Sbjct: 481 DVALVRALGGGYRAADDSSSQNSADAPLHDTQAKG 515 >gi|300312015|ref|YP_003776107.1| NodT family protein [Herbaspirillum seropedicae SmR1] Length = 495 Score = 275 bits (704), Expect = 4e-75, Method: Compositional matrix adjust. Identities = 181/462 (39%), Positives = 265/462 (57%), Gaps = 10/462 (2%) Query: 39 AGCASFTDLGPRAEPKSIDHYQSSQSLA---ASALQATWPSDQWWTVYGDAQLNSLIDEA 95 +GCASF +G + + + +SL+ A WP W +GDAQL +L+++A Sbjct: 27 SGCASFAGIGSDRQVAQAGDFATQRSLSDPNPGAPNGQWPGSDWVRQFGDAQLVALVEQA 86 Query: 96 LQSAPSMAVAKARLMKAEGAAQQQGAALLPEVSGNASLDHMKQSYNNGFPPALVPHGYN- 154 L S+PS+ A+AR+ A A+ +GA LLP V+ AS+ + S +PP P+G N Sbjct: 87 LTSSPSLQQARARIAAASALAESRGAPLLPSVNAEASVTRNQFSSTTIYPP---PYGGNW 143 Query: 155 -NETRTSLDFSYEIDFWGKNRAALAAATSELEASRADAAQARITLATSIASAYAELAQLY 213 NE + L+ YE+D W KN+AALA A S +A++A +AR+ L SI + Y++LA Y Sbjct: 144 YNEKKAGLNVGYELDLWNKNQAALAQAISSEKAAQASEQEARLALTASIVTVYSQLAAQY 203 Query: 214 AQRDTNEAALKVRVETLDLFKQRFANGLETRGSVMQMEARRAIAQADLKATDESIALQRN 273 A D ++ + R + +R GL+++ Q A A+A L ++ I L R Sbjct: 204 ALHDILQSTVDQRTSLEKITAERLRTGLDSQIERDQSRTSSADARAQLAQSEGQIVLLRQ 263 Query: 274 KLAALAGAGPDRGLQLARPQID-LSKPFGLPQQLPVELLGRRPDIVAARLRAEAAGKQIK 332 +L ALAG GPD GLQLA P + L+ P GLP +LP+ L+GRRPDIVAAR + EAA + + Sbjct: 264 QLGALAGKGPDYGLQLAPPALQGLATP-GLPAELPLNLMGRRPDIVAARWQVEAASRGVD 322 Query: 333 VARAAFYPNVNLSAYIGFQSLGLSMLTRSGSDIGSVGPAISLPIFNGGRLRGQFRSASAS 392 VA+A FYP++NLSA IGF +L S + S + GPAI+LPIF GG LR + AS Sbjct: 323 VAKARFYPDINLSAMIGFDTLLDSNPFTAASKSIAFGPAITLPIFEGGALRAGLKGEYAS 382 Query: 393 YDEAVANYDQAVTQALQDVADVGVSEKALTGRLADVKAAADAADEAYRIVSNRYNGGLAN 452 Y+ AVA Y++ + A DVA + A +L A AA+ AY + RY GL + Sbjct: 383 YELAVATYNKTLNDAYADVARQIAAIHATERQLPIRSEALQAAERAYALARERYRLGLVS 442 Query: 453 YLDVLNAQDTLISNLRQLSDLRSRMFTLDVALVRALGGGYRA 494 L +L+AQ +++ + + L+++ VAL +ALGGG+ A Sbjct: 443 QLTLLSAQTGVLAQRQAMVALQAQRRDQQVALYKALGGGFDA 484 >gi|300311423|ref|YP_003775515.1| NodT family protein [Herbaspirillum seropedicae SmR1] Length = 487 Score = 245 bits (625), Expect = 7e-66, Method: Compositional matrix adjust. Identities = 183/471 (38%), Positives = 262/471 (55%), Gaps = 4/471 (0%) Query: 32 VLAAAL-MAGCASFTDLGPRAEPKSIDHYQSSQSLAASALQATWPSDQWWTVYGDAQLNS 90 V+A +L MAGCA + P+A+ + + + Q++ +A +A WP WW YGDAQL+ Sbjct: 14 VIALSLTMAGCADQGGIQPQAQVRDVAVLDAGQAIRQAASEAGWPQRAWWQAYGDAQLDQ 73 Query: 91 LIDEALQSAPSMAVAKARLMKAEGAAQQQGAALLPEVSGNASLDHMKQSYNNGFPPALVP 150 L+ A+ PSMAVA AR+ +A+ A A LP + S+ S N + A Sbjct: 74 LVTRAVAGNPSMAVAAARVRQAQSMAVVAHAGELPSAQLDVSVGRKDWSDNVYYGAAFRN 133 Query: 151 HGYNNETRTSLDFSYEIDFWGKNRAALAAATSELEASRADAAQARITLATSIASAYAELA 210 N T T L SY +D W +N+ A A E+ A ADA A++ L ++ Y +LA Sbjct: 134 KLTWNNTAT-LGLSYNVDLWDRNKNATLRAQDEMHAVAADARAAQLDLEGNVVRTYVQLA 192 Query: 211 QLYAQRDTNEAALKVRVETLDLFKQRFANGLETRGSVMQMEARRAIAQADLKATDESIAL 270 Y D EA LK + LDL +RF GL T+ + Q E+ + ++A E+IAL Sbjct: 193 LQYGLLDNTEALLKEEQKILDLAHRRFKGGLGTQLEITQAESPLPETRRQIEALHENIAL 252 Query: 271 QRNKLAALAGAGPDRGLQLARPQIDLSKPFGLPQQLPVELLGRRPDIVAARLRAEAAGKQ 330 RN+LAAL GAGP + RP + L GLP LP +L+GRRPDI AAR R EAA K Sbjct: 253 IRNQLAALLGAGPGAADGVTRPGMQLQTSLGLPSALPADLVGRRPDISAARWRVEAAAKG 312 Query: 331 IKVARAAFYPNVNLSAYIGFQSLGLSMLT-RSGSDIGSV-GPAISLPIFNGGRLRGQFRS 388 I VA+AAFYPN+NL A IG + G + + S ++ + GPAISLPIF GGRLRGQ + Sbjct: 313 ISVAKAAFYPNINLLAGIGPAAAGGGLFSFLSAPNLQTTYGPAISLPIFEGGRLRGQLGA 372 Query: 389 ASASYDEAVANYDQAVTQALQDVADVGVSEKALTGRLADVKAAADAADEAYRIVSNRYNG 448 ASA YD V +Y+ ++QAL+ ++D V+ +++ + + + AA + I + Y Sbjct: 373 ASAGYDIEVEHYNALLSQALKGISDQVVTLQSVARQQEQAQQSVQAAQKNVDIATRAYQR 432 Query: 449 GLANYLDVLNAQDTLISNLRQLSDLRSRMFTLDVALVRALGGGYRAADNSK 499 GL +YL+VL+AQ + + + L+++ AL ALGGG A +K Sbjct: 433 GLTDYLNVLHAQTQWLRQQQIVQQLQAQRLAAYAALQTALGGGIEEAAPAK 483 >gi|300311155|ref|YP_003775247.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 491 Score = 171 bits (434), Expect = 1e-43, Method: Compositional matrix adjust. Identities = 140/422 (33%), Positives = 210/422 (49%), Gaps = 7/422 (1%) Query: 71 QATWPSDQWWTVYGDAQLNSLIDEALQSAPSMAVAKARLMKAEGAAQQQGAALLPEVSGN 130 +A P W + D L +LI +AL+++ + A R+ +A+ A L P + Sbjct: 58 EAASPPLPWRDYFTDPPLQALITQALENSRDLRTAALRVQEAQALYGIGHADLFPTIGAQ 117 Query: 131 ASLDHMKQSYN-NGFPPALVPHGYNNETRTSLDFSYEIDFWGKNRAALAAATSELEASRA 189 A LD + + N LV Y S S+EID WG+ R+ AA AS+A Sbjct: 118 AGLDRSRTPADLNLTRRPLVGSAYQAGLGLS---SWEIDLWGRLRSLDDAALESARASQA 174 Query: 190 DAAQARITLATSIASAYAELAQLYAQRDTNEAALKVRVETLDLFKQRFANGLETRGSVMQ 249 + ++L +A A+ ELA+L + + + R E+ +F +R G +R ++ Q Sbjct: 175 TRSAVALSLMAQVADAWLELAELDERLEIANQTIASRQESFRIFSRRVEVGATSRLNLTQ 234 Query: 250 MEARRAIAQADLKATDESIALQRNKLAALAGAGPDRGLQLARPQID-LSKPFGLPQQLPV 308 +E AQA + A + N L L G+ + AR +D + P L LP Sbjct: 235 IETLLTQAQALGAQLQQQRAQRLNALTLLVGSPVVLPVGQARAGLDAVFAP--LQPGLPA 292 Query: 309 ELLGRRPDIVAARLRAEAAGKQIKVARAAFYPNVNLSAYIGFQSLGLSMLTRSGSDIGSV 368 LL RRPD+ AA R AA I ARAAF+P+++L+ +G S LS L R GS + Sbjct: 293 LLLTRRPDVAAAEHRLMAANANIGAARAAFFPSISLTGNVGTASAELSGLFRDGSHAWTF 352 Query: 369 GPAISLPIFNGGRLRGQFRSASASYDEAVANYDQAVTQALQDVADVGVSEKALTGRLADV 428 P+I+LPIF GRLR + D AVA Y++ V A ++VAD + + LT +L Sbjct: 353 SPSITLPIFTAGRLRNNLALSEVRRDLAVAAYEKTVQTAFREVADALAARQWLTQQLEIA 412 Query: 429 KAAADAADEAYRIVSNRYNGGLANYLDVLNAQDTLISNLRQLSDLRSRMFTLDVALVRAL 488 ++A E R+ + RY+ G A +LDVL+AQ L+S +QL +R + + VAL AL Sbjct: 413 QSAVRVQRERARLSALRYDNGAAPFLDVLDAQRDLLSAQQQLVQIRRALLSSSVALYAAL 472 Query: 489 GG 490 GG Sbjct: 473 GG 474 >gi|300313898|ref|YP_003777990.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 486 Score = 164 bits (414), Expect = 2e-41, Method: Compositional matrix adjust. Identities = 138/429 (32%), Positives = 211/429 (49%), Gaps = 18/429 (4%) Query: 75 PSDQWWTVYGDAQLNSLIDEALQSAPSMAVAKARLMKAEGAAQQQGAALLPEVSGNASLD 134 P WW +GDAQLN+L +AL ++A+A ARL +A GAA P+V+ NA Sbjct: 52 PRGPWWERFGDAQLNALQQQALAGNQTLAIATARLAQARAQLNVTGAAQTPQVNLNARAA 111 Query: 135 HMKQSYNNGFPPALVPHGYNNETRTSLDF------SYEIDFWGKNRAALAAATSELEASR 188 + S N L + N + DF SYE+DF+G+ + ++ AA + E S Sbjct: 112 RSRISANR----PLTNYNSPNFSTVQNDFALGASVSYEVDFFGRVQRSVEAAGAAAEQSA 167 Query: 189 ADAAQARITLATSIASAYAELAQLYAQRDTNEAALKVRVETLDLFKQRFANGLETRGSVM 248 AD R+ L +A+ Y L +L + D A+ ++ + L+L R G S + Sbjct: 168 ADLENTRLLLTAELATNYFNLRELDVELDVVRRAIALQRQALELATARHDLGAT---SGL 224 Query: 249 QMEARRAIAQADLKATD---ESIALQRNKLAALAGAGPDRGLQLARPQIDLSKPFGLPQQ 305 M ++A+ + L D A + +A L G P + P + P +P Sbjct: 225 DMAQQQALLDSTLTQIDLLGRQRAQYEHAIATLTGT-PAPAFSIT-PSLTPITPPAIPVG 282 Query: 306 LPVELLGRRPDIVAARLRAEAAGKQIKVARAAFYPNVNLSAYIGFQSLGLSMLTRSGSDI 365 +P ++L RRPDI AA AA QI VA AA+YP+ L G S + L + S + Sbjct: 283 VPSDVLERRPDIAAAERAMAAANAQIGVASAAYYPSFMLQPSYGVDSRNWATLFNAPSLL 342 Query: 366 GSVGPAISLPIFNGGRLRGQFRSASASYDEAVANYDQAVTQALQDVADVGVSEKALTGRL 425 S+G + S +F+GGRLR + A Y+ VA+Y + V A+Q+V D AL Sbjct: 343 WSLGVSASQSLFDGGRLRAGVDFSQAGYEATVASYRRTVLTAMQEVEDGITGLAALERAY 402 Query: 426 ADVKAAADAADEAYRIVSNRYNGGLANYLDVLNAQDTLISNLRQLSDLRSRMFTLDVALV 485 A + A +A I S+RY GG YLDV+ AQ +L+++ RQ + L + + V L+ Sbjct: 403 AQSQLAIASARRVLEIASSRYEGGATPYLDVITAQQSLLNSERQAAQLMGQRLLVSVFLI 462 Query: 486 RALGGGYRA 494 +ALGG +++ Sbjct: 463 KALGGDWQS 471 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.314 0.127 0.353 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,332,685 Number of extensions: 48341 Number of successful extensions: 287 Number of sequences better than 1.0e-08: 20 Number of HSP's gapped: 234 Number of HSP's successfully gapped: 20 Length of query: 515 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 426 Effective length of database: 1,198,455 Effective search space: 510541830 Effective search space used: 510541830 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 133 (55.8 bits)