BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3223.p (432 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312562|ref|YP_003776654.1| guanine deaminase [Herbaspirill... 841 0.0 gi|300312557|ref|YP_003776649.1| hydroxy-atrazine ethyl amino hy... 99 7e-22 gi|300309804|ref|YP_003773896.1| hydroxydechloroatrazine ethylam... 56 5e-09 >gi|300312562|ref|YP_003776654.1| guanine deaminase [Herbaspirillum seropedicae SmR1] Length = 432 Score = 841 bits (2172), Expect = 0.0, Method: Compositional matrix adjust. Identities = 404/432 (93%), Positives = 422/432 (97%) Query: 1 MSTPLQAYRASLLYFTNDPAFDEHATQWHSDGLLLVQDGKVQAAGDYAQLSARLPAGTAI 60 M+TPL AYRASLLYFTNDPAFDEHAT+WHSDGLLLVQDGK++AAGDYA+LSA LP Sbjct: 1 MNTPLHAYRASLLYFTNDPAFDEHATRWHSDGLLLVQDGKIRAAGDYAELSASLPPEAVT 60 Query: 61 DDLRGKIICPGFIDTHVHYPQTDIIGSPAPGLLPWLDNYTFPAERRFQQPQHAAEVADFF 120 +DLRGKI+CPGFIDTHVHYPQTDIIGSPAPGLLPWLDNYTFPAERRFQQP+HAAEVADFF Sbjct: 61 EDLRGKILCPGFIDTHVHYPQTDIIGSPAPGLLPWLDNYTFPAERRFQQPEHAAEVADFF 120 Query: 121 LAELLRNGTTSAAVYCTVHPQSVDAFFHASHARNLRMVAGKCLMDRNAPDFLCDTAESGL 180 LAELLRNGTTSAAVYCTVHPQSVDAFF ASHARNLRMVAGKCLMDR+APDFL DTAESG+ Sbjct: 121 LAELLRNGTTSAAVYCTVHPQSVDAFFAASHARNLRMVAGKCLMDRHAPDFLRDTAESGV 180 Query: 181 RESEALIGRWHNKGRQHYAITVRFAPTSTPEQMKLAGELAAQYPDTYIQTHVAENTDEVQ 240 RESEALIGRWHNKGRQHYAITVRFAPTST EQMKLAGELAAQYPDTYIQTHVAENTDEV+ Sbjct: 181 RESEALIGRWHNKGRQHYAITVRFAPTSTHEQMKLAGELAAQYPDTYIQTHVAENTDEVK 240 Query: 241 WVKELYPDARSYLDVYDGYGLMRQRALYAHCIWLDDQDRARMAATQSAATLCPTSNLFLG 300 WVKELYPDARSYLDVYDGYGLMRQRALYAHCIWLDDQDRARMAATQSAATLCPTSNLFLG Sbjct: 241 WVKELYPDARSYLDVYDGYGLMRQRALYAHCIWLDDQDRARMAATQSAATLCPTSNLFLG 300 Query: 301 SGLFDFARADAARVPLSLATDVGGGTSFSMLQTMNELYKVARMGGTYLPAQRMFYLATLG 360 SGLFDFARADAAR+PLS+ATDVGGGTSFSMLQTMNELYKVARMGGTYLPAQRMFYLATLG Sbjct: 301 SGLFDFARADAARLPLSVATDVGGGTSFSMLQTMNELYKVARMGGTYLPAQRMFYLATLG 360 Query: 361 GARALQLEGTIGSFTPGCEADFIVLDPQATPLLARRSATTDSLEEQLFALAILGDDRAVA 420 GARALQLEGTIGSFTPGC+ADFIVLDPQATPLLARRSATT++LEEQLFALAILGDDRAVA Sbjct: 361 GARALQLEGTIGSFTPGCDADFIVLDPQATPLLARRSATTENLEEQLFALAILGDDRAVA 420 Query: 421 ATYAAGVKVHQR 432 ATY+AGV+VHQR Sbjct: 421 ATYSAGVRVHQR 432 >gi|300312557|ref|YP_003776649.1| hydroxy-atrazine ethyl amino hydrolase [Herbaspirillum seropedicae SmR1] Length = 458 Score = 99.0 bits (245), Expect = 7e-22, Method: Compositional matrix adjust. Identities = 102/375 (27%), Positives = 167/375 (44%), Gaps = 30/375 (8%) Query: 31 DGLLLVQDGKVQAAGDYAQLSARLPAGTAIDDLRGKIICPGFIDTHVHYPQ--TDIIGSP 88 DG L +++ ++ G A L I D R I+ PG I+TH H Q T I S Sbjct: 30 DGALYIRNNVIEQVGQTADLPQD---ADEIIDARHHIVLPGLINTHHHMYQSLTRAIPSA 86 Query: 89 APG-LLPWLDN-YTFPAERRFQQPQHAAEVADFFLAELLRNGTTSAAVYCTVHPQS--VD 144 G L WL Y A + Q + A +AEL+ +G T+++ + ++P +D Sbjct: 87 QNGELFNWLTKLYPIWAGLTPEMIQVSTLTA---MAELILSGCTTSSDHLYIYPNQTRLD 143 Query: 145 AFFHASHARNLRMVAGKCLMDRNA------PDFLCDTAESGLRESEALIGRWHNKGRQHY 198 A+ +R + M PD + + L++++ LI +H+ R H Sbjct: 144 DSLEAAARIGMRFHGARGAMSVGQSKGGLPPDRVVQDEAAILKDTQRLIETYHDHQR-HA 202 Query: 199 AITVRFAPTS----TPEQMKLAGELAAQYPDTYIQTHVAENTDEVQWVKELYPDARSYLD 254 + AP S + E M+ A +LA Y + TH+AEN +++ + +E + S + Sbjct: 203 MQRIVVAPCSPFSVSRELMREAADLARHY-GVSLHTHLAENVNDIAYSREKF--NMSPAE 259 Query: 255 VYDGYGLMRQRALYAHCIWLDDQDRARMAATQSAATLCPTSNLFLGSGLFDFARADAARV 314 + G + +AHC+ LD A T + CP SN+ L SG+ + A V Sbjct: 260 YAEDCGWVGHDVWHAHCVQLDAHGIDLFARTGTGVAHCPCSNMRLASGIAPIRKMLDAGV 319 Query: 315 PLSLATD-VGGGTSFSMLQTMNELYKVARM--GGTYLPAQRMFYLATLGGARALQLEGTI 371 P+ L D S ML + + + R+ G + A++ +ATLGGA+ L + I Sbjct: 320 PVGLGVDGSASNDSAHMLGEVRQAMLLQRVGFGPDAMTARQALEVATLGGAKVLNRD-DI 378 Query: 372 GSFTPGCEADFIVLD 386 G+ PG AD ++ D Sbjct: 379 GALKPGMSADVVMFD 393 >gi|300309804|ref|YP_003773896.1| hydroxydechloroatrazine ethylaminohydrolase [Herbaspirillum seropedicae SmR1] Length = 471 Score = 56.2 bits (134), Expect = 5e-09, Method: Compositional matrix adjust. Identities = 93/384 (24%), Positives = 154/384 (40%), Gaps = 59/384 (15%) Query: 29 HSDGLLLVQDGKVQAAGDYAQLSARLPAGTAIDDLRGKIICPGFIDTHVHYPQTDIIGSP 88 H+ + V DG + A G QL A LP G + D + P +++TH H Q+ + G P Sbjct: 23 HAGPDIRVSDGVIAAIG---QLDA-LP-GEELIDASHCVAYPAWVNTHHHLFQSLLKGDP 77 Query: 89 ---APGLLPWLDNYTFPAERRFQQPQHAAEVADFF-------LAELLRNGTTSAA----V 134 L PWL + F AD F + EL+R+G + A + Sbjct: 78 LGINATLTPWLAATPYRYRASFD--------ADLFRLAARIGMVELMRSGCGTIADHNYL 129 Query: 135 YCTVHP-QSVDAFFHASHARNLRMVAGKCLMDRN-------APDFLCDTAESGLRESEAL 186 Y P + + F + LR V + R + +T + L + + L Sbjct: 130 YYPGMPFDTSEILFEEAQQLGLRFVLCRGTATRTRQLEAELSSALRPETLDGFLADMQGL 189 Query: 187 IGRWHNKGRQHYAITVRFAPTS-----TPEQMKLAGELAAQYPDTYIQTHVAENT---DE 238 GR+H+ V APT+ P++++ +AA+ + +H++E D Sbjct: 190 AGRFHDPAPASMRRVV-MAPTTPLYSAAPDELREMA-VAAREMGLRLHSHLSETVGYQDS 247 Query: 239 VQWVKELYPDARSYLDVYDGYGLMRQRALYAHCIWLDDQDRARMAATQSAATLCPTSNLF 298 + P ++ + G + + +AH + LD ++ + T + CP SN Sbjct: 248 AHAMHGCRP-----VEFCERVGWLGRDVWFAHLVKLDAEEIQLLGGTGTGIAHCPQSNGR 302 Query: 299 LGSGLFDFARADAARVPLSLATDVGGGTSFSMLQTMNE-----LYKVARMGGTYLPAQRM 353 LGSG+ +AA VP+S+ D G S ++E L + AR G P+ R Sbjct: 303 LGSGIAPIRALEAAGVPVSIGVD--GAASNEAADMISETHAAWLMQRARRGQEATPSFRG 360 Query: 354 FYLATLGGARALQLEGTIGSFTPG 377 + GGA A +E + T G Sbjct: 361 GHFE--GGADAATVEDVVRWGTTG 382 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.134 0.409 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,460,745 Number of extensions: 64003 Number of successful extensions: 188 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 185 Number of HSP's successfully gapped: 3 Length of query: 432 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 344 Effective length of database: 1,203,190 Effective search space: 413897360 Effective search space used: 413897360 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 132 (55.5 bits)