BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3218.p (462 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312557|ref|YP_003776649.1| hydroxy-atrazine ethyl amino hy... 866 0.0 gi|300309804|ref|YP_003773896.1| hydroxydechloroatrazine ethylam... 257 1e-69 gi|300312562|ref|YP_003776654.1| guanine deaminase [Herbaspirill... 96 7e-21 gi|300311604|ref|YP_003775696.1| formiminoglutamate deiminase [H... 56 7e-09 >gi|300312557|ref|YP_003776649.1| hydroxy-atrazine ethyl amino hydrolase [Herbaspirillum seropedicae SmR1] Length = 458 Score = 866 bits (2238), Expect = 0.0, Method: Compositional matrix adjust. Identities = 414/457 (90%), Positives = 441/457 (96%) Query: 1 MQHPAHGKTLLIKNARVLVTMNETREEIANGALFIRDNVIEQVGQTQELPQEADEVIDAT 60 MQ A GKTLLI+NAR+LVTMNE+REEI +GAL+IR+NVIEQVGQT +LPQ+ADE+IDA Sbjct: 1 MQAQATGKTLLIRNARILVTMNESREEIPDGALYIRNNVIEQVGQTADLPQDADEIIDAR 60 Query: 61 HHVVIPGLINTHHHMYQSLTRAIPSAQNGELFHWLTNLYPIWAGLTPEMIQVSTLTAMAE 120 HH+V+PGLINTHHHMYQSLTRAIPSAQNGELF+WLT LYPIWAGLTPEMIQVSTLTAMAE Sbjct: 61 HHIVLPGLINTHHHMYQSLTRAIPSAQNGELFNWLTKLYPIWAGLTPEMIQVSTLTAMAE 120 Query: 121 LILSGCTTSSDHLYIYPNQTRLDDSLEAAARIGMRFHGARGAMSVGQSKGGLPPDRVVED 180 LILSGCTTSSDHLYIYPNQTRLDDSLEAAARIGMRFHGARGAMSVGQSKGGLPPDRVV+D Sbjct: 121 LILSGCTTSSDHLYIYPNQTRLDDSLEAAARIGMRFHGARGAMSVGQSKGGLPPDRVVQD 180 Query: 181 EEAILRDSRRLIETYHDPKRHAMQRIVVAPCSPFSVSRDLMREAADLARHYGVSLHTHLA 240 E AIL+D++RLIETYHD +RHAMQRIVVAPCSPFSVSR+LMREAADLARHYGVSLHTHLA Sbjct: 181 EAAILKDTQRLIETYHDHQRHAMQRIVVAPCSPFSVSRELMREAADLARHYGVSLHTHLA 240 Query: 241 ENLNDIAYSREKFNMTPAEYAEDCGWVGHDVWHAHCVQLDKHGIDLFARTGTGVAHCPCS 300 EN+NDIAYSREKFNM+PAEYAEDCGWVGHDVWHAHCVQLD HGIDLFARTGTGVAHCPCS Sbjct: 241 ENVNDIAYSREKFNMSPAEYAEDCGWVGHDVWHAHCVQLDAHGIDLFARTGTGVAHCPCS 300 Query: 301 NMRLASGIAPIRKMLDAGVAVGLGVDGSASNDSAHMLGEVRQAMLLQRVGFGPDAMTARQ 360 NMRLASGIAPIRKMLDAGV VGLGVDGSASNDSAHMLGEVRQAMLLQRVGFGPDAMTARQ Sbjct: 301 NMRLASGIAPIRKMLDAGVPVGLGVDGSASNDSAHMLGEVRQAMLLQRVGFGPDAMTARQ 360 Query: 361 ALEMATLGGARVLNRDDIGALKPGMSADVVLFDTRQIAFAGALHDPVAALVFCTPANVDY 420 ALE+ATLGGA+VLNRDDIGALKPGMSADVV+FDTRQIAFAGALHDPVAALVFCTPANVD Sbjct: 361 ALEVATLGGAKVLNRDDIGALKPGMSADVVMFDTRQIAFAGALHDPVAALVFCTPANVDC 420 Query: 421 SIINGRVIVRQGQCTTIELGAVLEQHNALALQLAQAV 457 SIINGRVIVR+GQ T+IELGAVLE+HN L+L LAQ V Sbjct: 421 SIINGRVIVREGQLTSIELGAVLERHNQLSLALAQGV 457 >gi|300309804|ref|YP_003773896.1| hydroxydechloroatrazine ethylaminohydrolase [Herbaspirillum seropedicae SmR1] Length = 471 Score = 257 bits (657), Expect = 1e-69, Method: Compositional matrix adjust. Identities = 170/464 (36%), Positives = 236/464 (50%), Gaps = 29/464 (6%) Query: 8 KTLLIKNARVLVT-MNETREEIANGALFIRDNVIEQVGQTQELPQEADEVIDATHHVVIP 66 T LI+N + ++T + A + + D VI +GQ LP E E+IDA+H V P Sbjct: 2 STFLIRNLQAIMTGLPGAAARHAGPDIRVSDGVIAAIGQLDALPGE--ELIDASHCVAYP 59 Query: 67 GLINTHHHMYQSLTRAIPSAQNGELFHWLT-NLYPIWAGLTPEMIQVSTLTAMAELILSG 125 +NTHHH++QSL + P N L WL Y A ++ +++ M EL+ SG Sbjct: 60 AWVNTHHHLFQSLLKGDPLGINATLTPWLAATPYRYRASFDADLFRLAARIGMVELMRSG 119 Query: 126 CTTSSDHLYIYPNQTRLDDS---LEAAARIGMRFHGARG-AMSVGQSKGGLPPDRVVEDE 181 C T +DH Y+Y D S E A ++G+RF RG A Q + L E Sbjct: 120 CGTIADHNYLYYPGMPFDTSEILFEEAQQLGLRFVLCRGTATRTRQLEAELSSALRPETL 179 Query: 182 EAILRDSRRLIETYHDPKRHAMQRIVVAPCSP-FSVSRDLMREAADLARHYGVSLHTHLA 240 + L D + L +HDP +M+R+V+AP +P +S + D +RE A AR G+ LH+HL+ Sbjct: 180 DGFLADMQGLAGRFHDPAPASMRRVVMAPTTPLYSAAPDELREMAVAAREMGLRLHSHLS 239 Query: 241 ENLNDIAYSREKFNMTPAEYAEDCGWVGHDVWHAHCVQLDKHGIDLFARTGTGVAHCPCS 300 E + + P E+ E GW+G DVW AH V+LD I L TGTG+AHCP S Sbjct: 240 ETVGYQDSAHAMHGCRPVEFCERVGWLGRDVWFAHLVKLDAEEIQLLGGTGTGIAHCPQS 299 Query: 301 NMRLASGIAPIRKMLDAGVAVGLGVDGSASNDSAHMLGEVRQAMLLQRV--------GF- 351 N RL SGIAPIR + AGV V +GVDG+ASN++A M+ E A L+QR F Sbjct: 300 NGRLGSGIAPIRALEAAGVPVSIGVDGAASNEAADMISETHAAWLMQRARRGQEATPSFR 359 Query: 352 ------GPDAMTARQALEMATLGGARVLNRDDIGALKPGMSADVVLFDTRQIAFAGALHD 405 G DA T + T GGARVL D + L+ G AD+ L+ + G LHD Sbjct: 360 GGHFEGGADAATVEDVVRWGTTGGARVLGLDALDGLQVGQQADIALYRLDDPRYFG-LHD 418 Query: 406 P-VAALVFCTPANVDYSIINGRVIVRQGQCTTI---ELGAVLEQ 445 P + + + + GR +VR+ + ELGA Q Sbjct: 419 PAIGPVASGGRPFLKAMWVGGRAVVREDAIPGVDLAELGAQARQ 462 >gi|300312562|ref|YP_003776654.1| guanine deaminase [Herbaspirillum seropedicae SmR1] Length = 432 Score = 95.9 bits (237), Expect = 7e-21, Method: Compositional matrix adjust. Identities = 101/376 (26%), Positives = 167/376 (44%), Gaps = 30/376 (7%) Query: 29 ANGALFIRDNVIEQVGQTQEL----PQEADEVIDATHHVVIPGLINTHHHMYQSLTRAIP 84 ++G L ++D I G EL P EA D ++ PG I+TH H Q T I Sbjct: 30 SDGLLLVQDGKIRAAGDYAELSASLPPEA-VTEDLRGKILCPGFIDTHVHYPQ--TDIIG 86 Query: 85 SAQNGELFHWLTNL-YPIWAGL-TPEMIQVSTLTAMAELILSGCTTSSDHLYIYPNQTRL 142 S G L WL N +P PE +AEL+ +G T+++ + ++P + Sbjct: 87 SPAPG-LLPWLDNYTFPAERRFQQPEHAAEVADFFLAELLRNGTTSAAVYCTVHPQS--V 143 Query: 143 DDSLEAAARIGMRFHGARGAMSVGQSKGGLPPDRVVEDEEAILRDSRRLIETYHDPKR-H 201 D A+ +R + M PD + + E+ +R+S LI +H+ R H Sbjct: 144 DAFFAASHARNLRMVAGKCLMDRHA------PDFLRDTAESGVRESEALIGRWHNKGRQH 197 Query: 202 AMQRIVVAPCSPFSVSRDLMREAADLARHY-GVSLHTHLAENLNDIAYSREKF--NMTPA 258 + AP S + + M+ A +LA Y + TH+AEN +++ + +E + + Sbjct: 198 YAITVRFAPTS----THEQMKLAGELAAQYPDTYIQTHVAENTDEVKWVKELYPDARSYL 253 Query: 259 EYAEDCGWVGHDVWHAHCVQLDKHGIDLFARTGTGVAHCPCSNMRLASGIAPIRKMLDAG 318 + + G + +AHC+ LD A T + CP SN+ L SG+ + A Sbjct: 254 DVYDGYGLMRQRALYAHCIWLDDQDRARMAATQSAATLCPTSNLFLGSGLFDFARADAAR 313 Query: 319 VAVGLGVDGSASNDSAHMLGEVRQAMLLQRVGFGPDAMTARQALEMATLGGARVLNRD-D 377 + + + D S ML + + + R+ G + A++ +ATLGGAR L + Sbjct: 314 LPLSVATD-VGGGTSFSMLQTMNELYKVARM--GGTYLPAQRMFYLATLGGARALQLEGT 370 Query: 378 IGALKPGMSADVVLFD 393 IG+ PG AD ++ D Sbjct: 371 IGSFTPGCDADFIVLD 386 >gi|300311604|ref|YP_003775696.1| formiminoglutamate deiminase [Herbaspirillum seropedicae SmR1] Length = 482 Score = 55.8 bits (133), Expect = 7e-09, Method: Compositional matrix adjust. Identities = 103/456 (22%), Positives = 173/456 (37%), Gaps = 66/456 (14%) Query: 29 ANGALFIRDNVIEQVGQTQEL-------------PQEADEVIDATHHVVIPGLINTHHHM 75 ANGALF ++ Q ++ L P A V+PG+ N H H Sbjct: 21 ANGALFCPLALLPQGWRSDVLLRWDGAGRLTHVTPDSARGSAALASGPVLPGMPNLHSHA 80 Query: 76 YQSLTRAIPSAQNG---ELFHWLTNLYPIWAGLTPEMIQVSTLTAMAELILSGCTTSSDH 132 +Q + + W +Y L P ++ E++ +G T+ + Sbjct: 81 FQRAMAGLAETMGSPADSFWSWRELMYRFAQRLQPRQLEAIARQTYIEMLKAGYTSVCEF 140 Query: 133 LYIY--PNQTRLDDSLEAAARIGMRFHGARGA----MSVGQSKGGL-------PPDRVVE 179 Y++ P+ D E A + ++ G G + V +GGL R V Sbjct: 141 HYLHHAPDGAPYADPAEMAWPL-LQAAGQSGIGMTLLPVLYQQGGLGGRPVSAEQRRFVA 199 Query: 180 DEEAILRDSRRLIETYHDPKRHAMQRIVVAPCSPFSVSRDLMRE-AADLARHYGVS---L 235 +L RL++ H P+ + M+R VAP S +V+ + E + LA G S + Sbjct: 200 APSWLLGLRERLLQ--HMPE-NDMRRYGVAPHSLRAVAPTGLEELLSGLAGQPGGSDAPI 256 Query: 236 HTHLAENLNDIAYSREKFNMTPAEYAEDCGWVGHDVWHAHCVQLDKHGIDLFARTGTGVA 295 H H+AE L ++ P ++ V H +D A +G V Sbjct: 257 HIHVAEQLREVEDCLAWSGQRPVQWLLSSQPVDARWCLVHATHMDDEEYRAVAGSGAVVG 316 Query: 296 HCPCSNMRLASGIAPIRKMLDAGVAVGLGVDGSASNDSAHMLGEVRQAMLLQRVGFGPDA 355 CP + L G+ +++D+ G+G D SN + + E+R QR Sbjct: 317 LCPTTEANLGDGVIDAPRLIDSAARWGIGSD---SNIAISLRAELRLLEYAQR------- 366 Query: 356 MTARQALEMATLGGARVLNR--------------DDIGALKPGMSADVVLFDTRQIAFAG 401 + R+ +A + V +R + L PG AD+V+ D + A Sbjct: 367 LHHRRRNVLAVIDAPAVADRLFCQALAGGAQAAGRAVAGLMPGQRADLVVLDPDHVNLAQ 426 Query: 402 -ALHDPVAALVFCT---PANVDYSIINGRVIVRQGQ 433 + ++ALVFC P D + GR +V++G+ Sbjct: 427 RSSTQLLSALVFCEHDGPMIGDV-YVGGRQVVKEGR 461 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.134 0.400 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,383,687 Number of extensions: 56000 Number of successful extensions: 179 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 173 Number of HSP's successfully gapped: 4 Length of query: 462 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 373 Effective length of database: 1,198,455 Effective search space: 447023715 Effective search space used: 447023715 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 132 (55.5 bits)