BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3145.p (488 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312485|ref|YP_003776577.1| outer membrane protein [Herbasp... 813 0.0 gi|300310691|ref|YP_003774783.1| outer membrane drug efflux lipo... 166 3e-42 gi|300313898|ref|YP_003777990.1| outer membrane drug efflux lipo... 154 2e-38 gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbas... 153 4e-38 gi|300309656|ref|YP_003773748.1| outer membrane drug efflux prot... 152 5e-38 >gi|300312485|ref|YP_003776577.1| outer membrane protein [Herbaspirillum seropedicae SmR1] Length = 491 Score = 813 bits (2099), Expect = 0.0, Method: Compositional matrix adjust. Identities = 438/487 (89%), Positives = 461/487 (94%) Query: 1 MYKTDSFFPRARLIALAAAALLAGCALGPEYAGPPEAAPQAAHRGHFVRAGDSATTAMPV 60 M KT S FP ARLIALAAAALLAGCALGP+YAGPPEAAPQAAH G FVR GDSATT P+ Sbjct: 4 MDKTSSSFPAARLIALAAAALLAGCALGPDYAGPPEAAPQAAHGGQFVRGGDSATTTAPI 63 Query: 61 NRWWQGLNDAELDSLIERALQANPNLGIARARLQQSRAGLELEQANSAPSVGASALAGHA 120 N+WWQGL DA+L SLIERALQANPNLGIARARLQQSRAGL LEQAN+APSVGASALAGHA Sbjct: 64 NQWWQGLGDAQLTSLIERALQANPNLGIARARLQQSRAGLALEQANAAPSVGASALAGHA 123 Query: 121 RLPPANLGALTGGSSSGNGSATSANLYSAGFDATWEADLFGAHRRAIQAAGATAQAAEAS 180 RLPPANLGALTGG+SSGNGS TSANLYS GFDA+WE DLFG RRA+QAA ATA AA+AS Sbjct: 124 RLPPANLGALTGGASSGNGSPTSANLYSLGFDASWEIDLFGGRRRAVQAATATADAAQAS 183 Query: 181 LADVQVSLAAEVANAYISLRDRQQRLALGEQAVQAQEQMLKLTSQRVARGTASSLEQIRI 240 LADVQVSLAAEVANAYISLRDRQQRLALG+QAVQAQEQML+LT QRV RGTASSL+Q+RI Sbjct: 184 LADVQVSLAAEVANAYISLRDRQQRLALGQQAVQAQEQMLQLTGQRVERGTASSLDQLRI 243 Query: 241 QNQLDASRADLIPLQADREAYLNQLATLLGQEPGTLDNELTATAPVPLPPQQVAVGDPVT 300 QNQLDASRADL+PLQADR+AYLNQLATLLGQEPGTLD EL AT PVPLPPQQVAVGDPV+ Sbjct: 244 QNQLDASRADLVPLQADRDAYLNQLATLLGQEPGTLDGELAATVPVPLPPQQVAVGDPVS 303 Query: 301 LLRRRPDVRAAERTLAADTAKIGQAEAARYPSLKLFGVIGLGGTHPSDLTRLDDFAAIGA 360 LLRRRPDVRAAERTLAADTAKIGQAEAARYPSLKLFGVIGLGGTHPSDLTRLDDFAA+GA Sbjct: 304 LLRRRPDVRAAERTLAADTAKIGQAEAARYPSLKLFGVIGLGGTHPSDLTRLDDFAALGA 363 Query: 361 PMLSWNFLDFGRAKARVTQAERVRDEAEMKYRQAVLEALRDAEDSLSRFRYGRVTVATLA 420 PMLSWNFLDFGRAKARVTQAERVRDEAEMKYRQ+VLEALRDAED+LSRFRYGRV+VAT+A Sbjct: 364 PMLSWNFLDFGRAKARVTQAERVRDEAEMKYRQSVLEALRDAEDALSRFRYGRVSVATIA 423 Query: 421 RTKASADRALELARQRYQAGTGTLIELLDTTRQQITAQQNLLQAEANLTRSFVGIQKALG 480 RTK SADRAL LARQRYQAGTGTLIE+LDTTRQQITAQQNLLQAEANLTRSFVGIQKALG Sbjct: 424 RTKLSADRALALARQRYQAGTGTLIEVLDTTRQQITAQQNLLQAEANLTRSFVGIQKALG 483 Query: 481 LGWSDQQ 487 LGWSD+Q Sbjct: 484 LGWSDEQ 490 >gi|300310691|ref|YP_003774783.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 498 Score = 166 bits (421), Expect = 3e-42, Method: Compositional matrix adjust. Identities = 152/492 (30%), Positives = 241/492 (48%), Gaps = 40/492 (8%) Query: 13 LIALAAAALLAGCALGPEYAGPPEAAPQAAHRGHFVRAGDSATTAMPVNR---WWQGLND 69 ++ + A LL GC L P+Y P AP AAH + ++ PV W D Sbjct: 15 MLPVMLAMLLGGCTLMPDYHRPD--APVAAHFAGADSKNAAGASSAPVVADIGWRDVFTD 72 Query: 70 AELDSLIERALQANPNLGIARARLQQSRAGLELEQANSAPSVGASALAGHARLPPANLGA 129 L +I AL+ N +L +A ++++RA +++A PS+ A+ +R P Sbjct: 73 PSLQQVIALALENNRDLRVAVLNIEKARAQYGVQRAALFPSIKAATSETASRTP------ 126 Query: 130 LTGGSSSGNGSATSANLYSA--GFDATWEADLFGAHR----RAIQAAGATAQAAEASLAD 183 G S GSA ++ Y+A GF A +E DLFG R +A+Q+ +TA+A ++ Sbjct: 127 ---GDLSSTGSALTSRSYTATLGFSA-YELDLFGRVRSLKEQALQSFLSTAEARRST--- 179 Query: 184 VQVSLAAEVANAYISLRDRQQRLALGEQAVQAQEQMLKLTSQRVARGTASSLEQIRIQNQ 243 Q+SL AEVA A+++L Q RL L + +++Q LT + G++S+L + Q Sbjct: 180 -QISLIAEVATAWLTLASDQDRLRLAQDTLESQSSSYALTQRSFELGSSSALTLRQAQTS 238 Query: 244 LDASRADLIPLQADREAYLNQLATLLGQE-------PGTLDNELTATAPV-PLPPQQVAV 295 ++++R D+ A + N L L+G++ G D L A +P+ +PP Sbjct: 239 VESARVDVESYTAQVDQDRNALVLLVGKDLPTALLPQGLPDTRLAAASPLATIPP----- 293 Query: 296 GDPVTLLRRRPDVRAAERTLAADTAKIGQAEAARYPSLKLFGVIGLGGTHPSDLTRLDDF 355 G P LL+RR D+ AER L A A IG A AA YPS+ L G + L + Sbjct: 294 GLPSDLLQRRADIVQAERDLRAANANIGAARAAFYPSITLTVNAGTASARLAGLFKGGSG 353 Query: 356 AAIGAPMLSWNFLDFGRAKARVTQAERVRDEAEMKYRQAVLEALRDAEDSLS-RFRYGRV 414 + P +S D G +A + A RD + +Y +A+ A R+ D+L+ R GR Sbjct: 354 SWSFVPQISLPIFDGGANQANLDIATVSRDISVAQYEKAIQTAFREVSDALAQRNTLGRQ 413 Query: 415 TVATLARTKASADRALELARQRYQAGTGTLIELLDTTRQQITAQQNLLQAEANLTRSFVG 474 A A +AS D A L++ RY +G + + +LD+ R +AQ+NL+ + + + V Sbjct: 414 LQAQEALVQAS-DEAFRLSQARYHSGVDSYLTVLDSQRTLYSAQKNLIGTQLSRWTNLVT 472 Query: 475 IQKALGLGWSDQ 486 K LG GW ++ Sbjct: 473 FYKTLGGGWVER 484 >gi|300313898|ref|YP_003777990.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 486 Score = 154 bits (388), Expect = 2e-38, Method: Compositional matrix adjust. Identities = 155/475 (32%), Positives = 228/475 (48%), Gaps = 38/475 (8%) Query: 24 GCALGPEYAGPPEAAPQAAHRGHFVRAGDSATTAMPVNRWWQGLNDAELDSLIERALQAN 83 GCA GP+Y P P A R+ A A P WW+ DA+L++L ++AL N Sbjct: 18 GCASGPDYTRPQVDMPAAWTSEAPWRSMQPAD-ATPRGPWWERFGDAQLNALQQQALAGN 76 Query: 84 PNLGIARARLQQSRAGLELEQANSAPSVGASALAGHARLPPANLGALTGGSSSGNGSATS 143 L IA ARL Q+RA L + A P V +A A +R+ AN LT +S +T Sbjct: 77 QTLAIATARLAQARAQLNVTGAAQTPQVNLNARAARSRIS-ANR-PLTNYNSPN--FSTV 132 Query: 144 ANLYSAGFDATWEADLFGAHRRAIQAAGATAQAAEASLADVQVSLAAEVANAYISLRDRQ 203 N ++ G ++E D FG +R+++AAGA A+ + A L + ++ L AE+A Y +LR+ Sbjct: 133 QNDFALGASVSYEVDFFGRVQRSVEAAGAAAEQSAADLENTRLLLTAELATNYFNLRELD 192 Query: 204 QRLALGEQAVQAQEQMLKLTSQRVARGTASSLEQIRIQNQLDASRADLIPLQADREAYLN 263 L + +A+ Q Q L+L + R G S L+ + Q LD++ + L R Y + Sbjct: 193 VELDVVRRAIALQRQALELATARHDLGATSGLDMAQQQALLDSTLTQIDLLGRQRAQYEH 252 Query: 264 QLATLLGQEPGTLDNELTATAPVPLPPQQVAVGDPVTLLRRRPDVRAAERTLAADTAKIG 323 +ATL G + T P+ P + VG P +L RRPD+ AAER +AA A+IG Sbjct: 253 AIATLTGTPAPAFSITPSLT---PITPPAIPVGVPSDVLERRPDIAAAERAMAAANAQIG 309 Query: 324 QAEAARYPSLKLFGVIGLGGTHPSDLTRLDDFAAIGAPMLSW--------NFLDFGRAKA 375 A AA YPS L G+ + + L AP L W + D GR +A Sbjct: 310 VASAAYYPSFMLQPSYGVDSRNWATL--------FNAPSLLWSLGVSASQSLFDGGRLRA 361 Query: 376 RVTQAERVRDEAEMKYRQAVLEALRDAEDSLSRFRYGRVTVATLARTKASADRALELARQ 435 V ++ + YR+ VL A+++ ED ++ A ASA R LE+A Sbjct: 362 GVDFSQAGYEATVASYRRTVLTAMQEVEDGITGLAALERAYAQSQLAIASARRVLEIASS 421 Query: 436 RYQAGTGTLIELLDTTRQQITAQQNLLQAEANLTRSF-------VGIQKALGLGW 483 RY+ G +++ ITAQQ+LL +E + V + KALG W Sbjct: 422 RYEGGATPYLDV-------ITAQQSLLNSERQAAQLMGQRLLVSVFLIKALGGDW 469 >gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 483 Score = 153 bits (386), Expect = 4e-38, Method: Compositional matrix adjust. Identities = 142/483 (29%), Positives = 228/483 (47%), Gaps = 38/483 (7%) Query: 15 ALAAAALLAGCALGPEYAGPPEAAPQAAHRGHFVRAGDSATTAMPVNRWWQGLNDAELDS 74 AL A +L GC+L P Y P +A ++++ AG + A+ + W Q D L Sbjct: 18 ALVMAGVLGGCSLAPTYERP-QAPVESSYPAD--AAGRAELQAVNLG-WRQFFPDQRLQQ 73 Query: 75 LIERALQANPNLGIARARLQQSRAGLELEQANSAPSVGASALAGHARLPPANLGALTGGS 134 LI AL+ N +L A ++++RA ++ +A+ P++ S A R T S Sbjct: 74 LIAAALENNRDLRTAVLNIEEARAQYQIARADLLPNLNLSGNAARTR---------TAAS 124 Query: 135 SSGNGSATSANLYSAGFDA-TWEADLFGAHRRAIQAAGATAQAAEASLADVQVSLAAEVA 193 S G T +N Y G +E D FG R AA A+ + E + Q+SL A+VA Sbjct: 125 QSLTGQPTISNSYQVGLAVPAYELDFFGRVRNLKDAALASYLSTEEAQKSAQISLVAQVA 184 Query: 194 NAYISLRDRQQRLALGEQAVQAQEQMLKLTSQRVARGTASSLEQIRIQNQLDASRADLIP 253 AY++ R ++L L ++ +++++ L L +R+ G +++L+ Q ++++R Sbjct: 185 QAYLAERSYAEQLKLADETLKSRQSNLDLAQKRLDVGASTALDFRLTQTLVESARVAKTQ 244 Query: 254 LQADREAYLNQLATLLGQEPGTLDNELTATAPVPLPPQQ----------VAVGDPVTLLR 303 L+ R N L L+G++ G L PP Q + G P LL Sbjct: 245 LERQRAQAENALTLLVGKKIGEL------------PPAQLLSDEKIVTDIPAGLPSDLLT 292 Query: 304 RRPDVRAAERTLAADTAKIGQAEAARYPSLKLFGVIGLGGTHPSDLTRLDDFAAIGAPML 363 RPD+R+AE++L A A IG A AA +P + L G G + L A P L Sbjct: 293 NRPDIRSAEQSLLAANANIGAARAAFFPRISLTGNFGSASSTLGGLFESGSGAWSFGPSL 352 Query: 364 SWNFLDFGRAKARVTQAERVRDEAEMKYRQAVLEALRDAEDSL-SRFRYGRVTVATLART 422 S DFGR A + AE +++A + Y +A+ A R+ D+L +R A A Sbjct: 353 SLPIFDFGRNSANLDLAEVRKNKAVVTYEKAIQTAFREVADALVARGSLDEQVKAQEAFL 412 Query: 423 KASADRALELARQRYQAGTGTLIELLDTTRQQITAQQNLLQAEANLTRSFVGIQKALGLG 482 A +R L+L R++ G + ++ LD R +AQQ L+QA + + + KALG G Sbjct: 413 AAQQER-LKLTDLRFKNGIASSLDQLDAQRDLFSAQQALIQARQLRLNNAIDLYKALGGG 471 Query: 483 WSD 485 ++ Sbjct: 472 LNE 474 >gi|300309656|ref|YP_003773748.1| outer membrane drug efflux protein [Herbaspirillum seropedicae SmR1] Length = 504 Score = 152 bits (385), Expect = 5e-38, Method: Compositional matrix adjust. Identities = 148/480 (30%), Positives = 220/480 (45%), Gaps = 36/480 (7%) Query: 24 GCALGPEYAGP----PEAAPQAAHRGHFVRAGDSATTAMPVN-----RWWQGLNDAELDS 74 GC++ P Y P P A +AA G TA P WW+ D +L++ Sbjct: 30 GCSVAPTYERPSVDTPAAFKEAAPATQTGENGSQWKTAQPAEDIARGEWWKIFGDEKLNA 89 Query: 75 LIERALQANPNLGIARARLQQSRAGLELEQANSAPSVGASALAGHARLPPANLGALTGGS 134 L + A QAN +L A ARL Q+RA +A P V A A R PA+ G Sbjct: 90 LEDTAQQANQDLKAAAARLGQARALTRDARAGYFPQVDAGVGATRQRPSPASQGLPADAD 149 Query: 135 SSGNGSATSANLYSAGFDATWEADLFGAHRRAIQAAGATAQAAEASLADVQVSLAAEVAN 194 + N LY A + ++E DLFG + AA A AQ +EA V ++L A+VA Sbjct: 150 TRPN------TLYRAQVNVSYEVDLFGRVASTVDAATADAQRSEALFHSVLLALQADVAQ 203 Query: 195 AYISLRDRQQRLALGEQAVQAQEQMLKLTSQRVARGTASSLEQIRIQNQLDASRADLIPL 254 Y +R+ AL V +++ L+L +R G S ++ R + +L +++++ + + Sbjct: 204 QYFLVRELDAAAALYRGTVDLRQESLRLVQRRFDEGDISEVDVARARAELASAQSEALGI 263 Query: 255 QADREAYLNQLATLLGQEPGTLDNELTATAPVPLPPQ-------QVAVGDPVTLLRRRPD 307 R + LA LLG+ P A LPPQ V G P LL RRPD Sbjct: 264 ARQRATAEHALAILLGRTP----------AEFSLPPQPLTRLAVTVPPGLPSALLERRPD 313 Query: 308 VRAAERTLAADTAKIGQAEAARYPSLKLFGVIGLGGTHPSDLTRLDDFAAIGAPM----L 363 + AAER +AA A++G A++A +PSL L G +G DL + P+ L Sbjct: 314 IAAAERAMAAANARVGVAKSAFFPSLNLTGGLGYESAQLGDLFNWSSRTFLLGPLVGTAL 373 Query: 364 SWNFLDFGRAKARVTQAERVRDEAEMKYRQAVLEALRDAEDSLSRFRYGRVTVATLARTK 423 S D GR +A V +A +E YR VL A R+ ED+L+ R Sbjct: 374 SMPLFDGGRRQAGVDRARASYEEQVASYRGTVLNAFREVEDNLANLRILDQQNRAQDEAV 433 Query: 424 ASADRALELARQRYQAGTGTLIELLDTTRQQITAQQNLLQAEANLTRSFVGIQKALGLGW 483 A++ RA +L+ +Y+ G + ++D R + Q+ + + RS V + +ALG GW Sbjct: 434 AASARAAKLSHTQYREGAVSYFSVIDADRTVLQQQRASVTLDGERARSTVNLIRALGGGW 493 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.128 0.358 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,145,041 Number of extensions: 41120 Number of successful extensions: 307 Number of sequences better than 1.0e-08: 19 Number of HSP's gapped: 244 Number of HSP's successfully gapped: 19 Length of query: 488 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 399 Effective length of database: 1,198,455 Effective search space: 478183545 Effective search space used: 478183545 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 132 (55.5 bits)