BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3144.p (1261 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312484|ref|YP_003776576.1| serine endopeptidase [Herbaspir... 1687 0.0 >gi|300312484|ref|YP_003776576.1| serine endopeptidase [Herbaspirillum seropedicae SmR1] Length = 1371 Score = 1687 bits (4368), Expect = 0.0, Method: Compositional matrix adjust. Identities = 920/1378 (66%), Positives = 1038/1378 (75%), Gaps = 124/1378 (8%) Query: 1 MKNKKKPSRQATMTSTAGLQKKRLCGAVLLTLG----LPASAQSYVNYDGSSSTDVAAAV 56 MK +K + A +T++A +K+ L AVLLTLG PASAQSYVNYDG+S+ D +AA+ Sbjct: 1 MKKSRKSPQNAALTTSAVFRKQGLYAAVLLTLGPALGTPASAQSYVNYDGASTADASAAL 60 Query: 57 ASWAANSEFKTDWGLGAMNAQYAYAAGFSGLGIKLGSVDSGLLLTHAEFVSRNVKALAVI 116 A+W ++EFK DWGLGAMNAQYAY AG++G GIKLG+VDSGLLLTH EF RNVKALAV Sbjct: 61 ATWTGHAEFKADWGLGAMNAQYAYVAGYTGQGIKLGAVDSGLLLTHNEFARRNVKALAVT 120 Query: 117 GTYANDGSQYQNGSQPWKAGDAFSTTGAVSTLNDKHGSHVSGSIAAAKNGAGIMGVAFAS 176 G YANDG QYQ+GSQ WKAGDAFS GA+S LNDKHGSHVSG+IAAAKNG GIMGVAFAS Sbjct: 121 GAYANDGYQYQDGSQAWKAGDAFSVPGALSPLNDKHGSHVSGTIAAAKNGDGIMGVAFAS 180 Query: 177 DYYITNTNGTDSSVYGVNMDYNYFKAAYGNLASAGVRVINSSWGSPDSRDDFGSIGGVAA 236 DYYITNTNGTDSSVYGVNMDYNYFKAAYGNLASAGVR INSSWG+PDSRDDFGSI GVAA Sbjct: 181 DYYITNTNGTDSSVYGVNMDYNYFKAAYGNLASAGVRAINSSWGNPDSRDDFGSISGVAA 240 Query: 237 AYQRLQGGAKKSWLDAAADVAQASNTLMVWAAGNSSKSNVNIRSALPYFRPELEQNWIAV 296 AYQRLQGG KKSWLDAAADVAQASNTL+VWAAGN+SK+NVNIRSALPYFRPELEQNWIAV Sbjct: 241 AYQRLQGGGKKSWLDAAADVAQASNTLLVWAAGNASKANVNIRSALPYFRPELEQNWIAV 300 Query: 297 AAVNASSVLPSFSNQCGLAKYWCLSAPGSAIPSLNITSDTALVNASGTSMAAPHVTGALG 356 AA+N SS+LPSFSNQCGLAKYWC+SAPGS I SLNITSDTALVNASGTSMAAPHVTGALG Sbjct: 301 AALNTSSMLPSFSNQCGLAKYWCVSAPGSGITSLNITSDTALVNASGTSMAAPHVTGALG 360 Query: 357 VLMERYPDLDNQAIRTILLTTSTHLGGGAVDVPNAMFGWGLPDLKKALNGPAQLLGVFNA 416 VLM+RYPDLDNQAIRTILL+T+ HLG GA D PN+MFGWG+PDL KAL GP QLLGVF A Sbjct: 361 VLMQRYPDLDNQAIRTILLSTAKHLGTGAADAPNSMFGWGVPDLNKALYGPGQLLGVFKA 420 Query: 417 NIGSGRSDTWSNDISEAALVQRKSEEKAQLAAWQALSVSTLQAAVDSATAQVTSRVNSGY 476 NI +G +DTWSNDISEAALVQRKSE+KAQLAAWQALS STLQ+AVDSA+AQV ++V+SGY Sbjct: 421 NIAAGSTDTWSNDISEAALVQRKSEDKAQLAAWQALSASTLQSAVDSASAQVKAQVDSGY 480 Query: 477 DAALALVQTRAQRNATYQASKKKEDNTAFQNADKAVAANPLAAAILKQSGTGALLSKEQL 536 DAALALVQTRAQ NA YQ+S K+ED TAFQNADKAVAAN LAAAILKQSG+GALLSK+QL Sbjct: 481 DAALALVQTRAQLNAAYQSSNKREDKTAFQNADKAVAANALAAAILKQSGSGALLSKDQL 540 Query: 537 LTYVLSTDTDMVAANLALGNYKGQTAYLDAVQKKTDADYVGTLVKSGAGVLTLSGNNSYS 596 L Y+L+ D+ + AANLALGNY GQ YL A QKKTDADYVG+LVK+G G LTLSGNNSY+ Sbjct: 541 LAYMLTVDSGVAAANLALGNYNGQAVYLAAAQKKTDADYVGSLVKTGDGSLTLSGNNSYT 600 Query: 597 GGTRLEGGTLAVASATALGSGALGMNDGTTLRAAADGLALGNAVSLHGEGRIDTQAFTLT 656 GGTRLEGGTL V S+TALG+G L M+DG TLRAAAD LAL NAVS++G GRIDTQAF LT Sbjct: 601 GGTRLEGGTLGVGSSTALGTGGLAMSDGVTLRAAADNLALNNAVSINGAGRIDTQAFNLT 660 Query: 657 LNAGIADG--------SGKGSLVKQGE-----------GTLRVLGPVRYSGNTTIAA--- 694 LN GIADG G+G+L G GTL + V+ +G Sbjct: 661 LNNGIADGRVPGSLVKQGEGTLRVLGTVSYSGNTTIAAGTLSLPTYVQTAGQILTVGVAS 720 Query: 695 ----GTLSVP---SFAQTSEQILTVGVASATQYG---KLAVTGTASFASGARLAV----- 739 G L+V SFA S+ L V VA A G +LA TA S RLAV Sbjct: 721 ATQYGKLAVTGAASFA--SDAALAVDVAKAATLGNGQRLAGVITAGSLSAPRLAVSDNSV 778 Query: 740 ---------------DVSKAA--------TLARGQTLGGVITAGSLSAPSLNVSDNSLLF 776 D+ A TLA+ Q G + S P + +S Sbjct: 779 LFDFQPVVASNAVDLDIVSAVSINKVVLDTLAQAQ---GPANTATTSTPVASALASSTPV 835 Query: 777 DFRPVVANNAVDLNIVDVVSIQQVVKDTLAPAT-VAAAPAAVITPTASTPTASTPTASTP 835 PVV+ A + + +T A +T V +APA+ TP ASTP S P ++TP Sbjct: 836 ASTPVVSAPASNTPAASTPVVSVPASNTPAASTPVVSAPASN-TPAASTPVVSAPASNTP 894 Query: 836 AASTP----------AASTPAASTPVASTPVASTPVASKPAASTPVASTLVAPPPAFRTS 885 AASTP AASTP S PV++TP ASTPV S P ++TPVAST V PA T Sbjct: 895 AASTPVVSVPASNTSAASTPLVSVPVSNTPAASTPVVSVPVSNTPVASTPVVSVPASNTP 954 Query: 886 LAIASAVAATPVA------------------------------------------PVLDR 903 A ++ VA+ P + P+LDR Sbjct: 955 -AASTPVASAPASGTPASSTAAPSASAAPAVVPAVAPAARIPSVVAAATAAATVAPILDR 1013 Query: 904 QIQHPSSGDMAQVVTALGRLPDAASLMRAASQTLPRNDSASAIRGSQASLNRVIATRVET 963 QIQ PSS DMAQVVTALGRLPDAAS++RAASQTLPRN S +AIRG ASL+RVIATR++ Sbjct: 1014 QIQQPSSADMAQVVTALGRLPDAASVLRAASQTLPRNVSDNAIRGVLASLDRVIATRLDL 1073 Query: 964 GSSGIDGVGAGSGLAYGEAGADRQVWIMPFESRTNQGDRGGASGFSASTWGMAAGAEMEL 1023 G+ G GSGL YGEAG DRQVW+MPFESRTNQGDR G SGFSASTWGMA GAEM Sbjct: 1074 GNLAGSATGTGSGLGYGEAGGDRQVWLMPFESRTNQGDRDGDSGFSASTWGMATGAEMAW 1133 Query: 1024 ERGRVGISYAYAGTRTSGNTAITGTGSRGRIESNMLALYGSVALGELALGWQADLGWNGN 1083 + GRVG+SYAYA T SGNTAITG GSR RIESN++ALYGSV LG+LAL WQAD GWN N Sbjct: 1134 DSGRVGLSYAYASTSASGNTAITGAGSRSRIESNLVALYGSVPLGKLALSWQADAGWNSN 1193 Query: 1084 RLSRDLQFGGLNRIASSRYHSWSAHVGANVSHTLPLSEVLGLVPALQLDYTRLQSPAYAE 1143 RL+R++QFGGL+RIASSRYH+WSAH+GANVSHTLPLSE L +VPALQLDYTR+QSP+YAE Sbjct: 1194 RLAREMQFGGLHRIASSRYHTWSAHLGANVSHTLPLSEALSVVPALQLDYTRMQSPSYAE 1253 Query: 1144 SGAGDLSLSVQGKRSQALLLGVSTRLNYAVTAASQISSYLGASYDAINDRDDLVATYAGA 1203 SGAGDLSLSVQGKR+QALLLGV TRLNYA++ SQIS+++GASYD INDR DLVATYAG Sbjct: 1254 SGAGDLSLSVQGKRTQALLLGVGTRLNYALSTTSQISTHVGASYDVINDRGDLVATYAGT 1313 Query: 1204 PGQAFTAPGTARSAWLFKAGVSYRYQLMATADITLRLDAEGRSGFINQSAALKAGWRF 1261 PGQAFTAPG+ARSAWLFKAGVSYR+QL ADI+LRLDAEGRSGFINQSAALKA WRF Sbjct: 1314 PGQAFTAPGSARSAWLFKAGVSYRFQLTTEADISLRLDAEGRSGFINQSAALKASWRF 1371 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.312 0.125 0.354 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 3,647,378 Number of extensions: 157491 Number of successful extensions: 632 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 613 Number of HSP's successfully gapped: 4 Length of query: 1261 Length of database: 1,619,870 Length adjustment: 96 Effective length of query: 1165 Effective length of database: 1,165,310 Effective search space: 1357586150 Effective search space used: 1357586150 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 136 (57.0 bits)