BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3135.p (399 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312475|ref|YP_003776567.1| metal-dependent amidase/aminoac... 757 0.0 gi|300310161|ref|YP_003774253.1| aminohydrolase [Herbaspirillum ... 362 e-101 gi|300310472|ref|YP_003774564.1| hippurate hydrolase [Herbaspiri... 343 2e-95 >gi|300312475|ref|YP_003776567.1| metal-dependent amidase/aminoacylase/carboxypeptidase [Herbaspirillum seropedicae SmR1] Length = 399 Score = 757 bits (1955), Expect = 0.0, Method: Compositional matrix adjust. Identities = 358/399 (89%), Positives = 386/399 (96%) Query: 1 MNHPHEHQLCEVADLHDLKEEITGLRRHIHQHPELSFEEVDTAALVAARLEEWGYAVTRH 60 M+H H LCEV DLHD+++EIT +RRH+HQHPELSFEEVDTAALVA RLE+WGYAVTRH Sbjct: 1 MSHAHPGHLCEVGDLHDIQDEITAIRRHLHQHPELSFEEVDTAALVAQRLEQWGYAVTRH 60 Query: 61 IGGNGLVATLRVGSSTRSIGLRADMDALPIQEETGLDWASIKPGAMHACGHDGHTAMLLG 120 IGGNGLVATLRVGSS RSIGLRADMDALPIQEETGLDWAS+KPGAMHACGHDGHTAMLLG Sbjct: 61 IGGNGLVATLRVGSSARSIGLRADMDALPIQEETGLDWASVKPGAMHACGHDGHTAMLLG 120 Query: 121 AAKHLARTRRFDGTLNLIFQPAEEAGFDSGAQKMLADGLFERFPCDAVFGIHNHPGVEEG 180 AA+HLARTRRFDGTLNLIFQPAEEAGFDSGAQKMLADGLFERFPC+AVFGIHNHPGVE G Sbjct: 121 AARHLARTRRFDGTLNLIFQPAEEAGFDSGAQKMLADGLFERFPCEAVFGIHNHPGVEAG 180 Query: 181 TFMFRSGPFMAACDTVKIRITGRGSHAARPHLSVDPVVAAASVVMALQTVVARNVDPMET 240 TFMFRSGPFMAACDTVKIRITGRGSHAARPHLSVDPVV AAS+VMALQTVV+RN+DPM++ Sbjct: 181 TFMFRSGPFMAACDTVKIRITGRGSHAARPHLSVDPVVVAASLVMALQTVVSRNIDPMDS 240 Query: 241 AVVTVGSLHAGRASNVIPEFATLELSVRSFKPEVRELLEQRIRALVSSHAESYGAQAQIE 300 AVVTVGSLHAG+ASNVIPEFAT+ELSVRSFKPEVRELLEQRIRALVS+HA+SYGAQA+I+ Sbjct: 241 AVVTVGSLHAGKASNVIPEFATMELSVRSFKPEVRELLEQRIRALVSTHAQSYGAQAEID 300 Query: 301 YLRGYPVLVNSQAETDFARSVAEELVGPDKVIAPFGPIAGSEDFAYFLQQRPGCFLRVGN 360 YLRGYPVLVNS AETDFARSVAEELVGP+KVIAPFGPIAGSEDFAYFL+QRPGCFLRVGN Sbjct: 301 YLRGYPVLVNSDAETDFARSVAEELVGPEKVIAPFGPIAGSEDFAYFLRQRPGCFLRVGN 360 Query: 361 GRGQPMLHNAGYDFNDANIPVGAAYWTRLVERWMPAKAD 399 G+G+PMLHNAGYDFNDANIP+GAAYWTRLVERWMP + + Sbjct: 361 GQGKPMLHNAGYDFNDANIPIGAAYWTRLVERWMPLQGE 399 >gi|300310161|ref|YP_003774253.1| aminohydrolase [Herbaspirillum seropedicae SmR1] Length = 402 Score = 362 bits (928), Expect = e-101, Method: Compositional matrix adjust. Identities = 185/389 (47%), Positives = 246/389 (63%), Gaps = 13/389 (3%) Query: 21 EITGLRRHIHQHPELSFEEVDTAALVAARLEEWGYAVTRHIGGNGLVATLRVGSSTRSIG 80 ++ +RRH+H HPEL FEE TA+ VA L +G V +IGG G+VATLR G STR+I Sbjct: 16 DLVSIRRHLHAHPELRFEEKRTASFVADMLRSYGLTVAENIGGYGVVATLRKGKSTRAIA 75 Query: 81 LRADMDALPIQEETGLDWASIKPGAMHACGHDGHTAMLLGAAKHLARTRRFDGTLNLIFQ 140 LRADMDALP+ E+ S G MHACGHDGHT MLLGAA+ L+R FDGT++ +FQ Sbjct: 76 LRADMDALPMSEQNDFSHISTCAGKMHACGHDGHTTMLLGAARRLSREVEFDGTVHFVFQ 135 Query: 141 PAEEAGFDSGAQKMLADGLFERFPCDAVFGIHNHPGVEEGTFMFRSGPFMAACDTVKIRI 200 PAEE G +GA+ M+ DGLFERFP DA+FG+HN PG+ G+F R GP MA+ +T + Sbjct: 136 PAEEGG--AGARLMIEDGLFERFPADAIFGVHNWPGLPAGSFGLRPGPLMASSNTFSATL 193 Query: 201 TGRGSHAARPHLSVDPVVAAASVVMALQTVVARNVDPMETAVVTVGSLHAGRASNVIPEF 260 GRG+H A+PH S+DPV+AAA + +A Q++V RN++P AV++V LH G A NVIPE Sbjct: 194 FGRGAHGAQPHRSIDPVLAAAQLTLAWQSIVTRNINPNHRAVISVTQLHTGTADNVIPEQ 253 Query: 261 ATLELSVRSFKPEVRELLEQRIRALVSSHAESYGAQAQIEYLRGYPVLVNSQAETDFARS 320 ATL +VRSF E +L+E R+ A+ + + + + Q + R YP +VN T A Sbjct: 254 ATLSGTVRSFDAETLDLIEHRMAAIAEAIGQMFDLRVQFHFERLYPAVVNHPEATQQAMQ 313 Query: 321 VAEELVGPDKVIAPFGPIAGSEDFAYFLQQRPGCFLRVGNG---RGQPM--------LHN 369 VG ++V P SEDFA++LQ +PGC+ +GNG G+ LH+ Sbjct: 314 AMMAAVGAEQVDGDTEPAFASEDFAFYLQHKPGCYAFLGNGVIADGKQAGSQATSRELHS 373 Query: 370 AGYDFNDANIPVGAAYWTRLVERWMPAKA 398 YDFND I G AYW LV +++ A Sbjct: 374 PFYDFNDDIIDAGVAYWVSLVRQYLGVAA 402 >gi|300310472|ref|YP_003774564.1| hippurate hydrolase [Herbaspirillum seropedicae SmR1] Length = 403 Score = 343 bits (879), Expect = 2e-95, Method: Compositional matrix adjust. Identities = 176/392 (44%), Positives = 243/392 (61%), Gaps = 5/392 (1%) Query: 6 EHQLCEVADLHDLKEEITGLRRHIHQHPELSFEEVDTAALVAARLEEWGYAVTRHIGGNG 65 EH + LH + GLRR +H HPEL FEE TA +V L+ GY VTR +GG G Sbjct: 2 EHTGHQHDTLHQHRAHWAGLRRDLHAHPELRFEEHRTADVVVRELQSLGYTVTRGLGGTG 61 Query: 66 LVATLRVGSSTRSIGLRADMDALPIQEETGLDWASIKPGAMHACGHDGHTAMLLGAAKHL 125 +VA+L R I LRAD+DALPI E AS G MHACGHDGHT MLLGAA+ L Sbjct: 62 VVASLAGADPQRGIVLRADLDALPIVEANDFAHASCTHGVMHACGHDGHTVMLLGAARLL 121 Query: 126 ARTRRFDGTLNLIFQPAEEAGFDSGAQKMLADGLFERFPCDAVFGIHNHPGVEEGTFMFR 185 + G+++ +FQP EE G +GA+KM+ DGLFE+FP +AVFG+HN PG+ G F R Sbjct: 122 KQGPMLPGSVHFVFQPGEEGG--AGARKMIDDGLFEQFPTEAVFGMHNWPGLPAGQFGLR 179 Query: 186 SGPFMAACDTVKIRITGRGSHAARPHLSVDPVVAAASVVMALQTVVARNVDPMETAVVTV 245 GP MAA +KI ITG+G+HAA+PHL +DP+ A S+V+ QT+ AR+ DP++ AV++V Sbjct: 180 PGPIMAAGSRLKITITGKGAHAAQPHLGLDPIPLACSMVLQCQTIAARHKDPVDPAVISV 239 Query: 246 GSLHAGRASNVIPEFATLELSVRSFKPEVRELLEQRIRALVSSHAESYGAQAQIEYLRGY 305 HAG NVIP+ A L ++R+ +++ L++ IR + + AE+YGA+ ++E+ + Y Sbjct: 240 CMFHAGDTDNVIPDRAELRGTIRTLSSTLQQKLQEDIRRMCHALAEAYGAKVEVEFFQYY 299 Query: 306 PVLVNSQAETDFARSVAEELVGPDKVIAPFGPIAGSEDFAYFLQQRPGCFLRVGNG---R 362 P +N+ AETDF V + G D++ SEDF + L++RPG ++ +GN R Sbjct: 300 PATINTPAETDFCERVIRQTFGDDRIRTGIPANMTSEDFGFMLEERPGAYVLIGNAPQDR 359 Query: 363 GQPMLHNAGYDFNDANIPVGAAYWTRLVERWM 394 LH+ YDFND I G YW L + + Sbjct: 360 ASHSLHHPHYDFNDDIIEAGVRYWVALAQGYF 391 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.135 0.409 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,317,753 Number of extensions: 57968 Number of successful extensions: 165 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 161 Number of HSP's successfully gapped: 3 Length of query: 399 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 312 Effective length of database: 1,207,925 Effective search space: 376872600 Effective search space used: 376872600 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)