BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3116.p (522 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312450|ref|YP_003776542.1| D-galactarate dehydratase [Herb... 1053 0.0 gi|300313170|ref|YP_003777262.1| altronate hydrolase [Herbaspiri... 279 4e-76 >gi|300312450|ref|YP_003776542.1| D-galactarate dehydratase [Herbaspirillum seropedicae SmR1] Length = 522 Score = 1053 bits (2723), Expect = 0.0, Method: Compositional matrix adjust. Identities = 507/522 (97%), Positives = 517/522 (99%) Query: 1 MSNDTTASQAFTPRYIMMNDTDNVAIVVNDGGLPAGTVFPDGLTLIDRVPQGHKIALRDL 60 MS DT+ASQA PRYIMMNDTDNVAIVVNDGGLPAGTVFPDGLTL+DRVPQGHKIALRDL Sbjct: 1 MSTDTSASQASAPRYIMMNDTDNVAIVVNDGGLPAGTVFPDGLTLVDRVPQGHKIALRDL 60 Query: 61 KQGDAIVRYDVAIGYAVRDIPKGSWIEESLVQMPPARELDNLPIATKKPAPQPPLEGYTF 120 KQG+AIVRYDVAIGYAVRDIPKG WIEESLVQMPPARELDNLPIATKKPAPQPPLEGYTF Sbjct: 61 KQGEAIVRYDVAIGYAVRDIPKGGWIEESLVQMPPARELDNLPIATKKPAPQPPLEGYTF 120 Query: 121 EGYRNADGSVGTRNLLAITTTVQCVAGVVEHAVKRIRAELLPKYPNVEDVVALEHTYGCG 180 EGYRNADGSVGTRNLLAITTTVQCVAGVVEHAVKRIRAELLPKYPNVEDVVALEHTYGCG Sbjct: 121 EGYRNADGSVGTRNLLAITTTVQCVAGVVEHAVKRIRAELLPKYPNVEDVVALEHTYGCG 180 Query: 181 VAIDAPNAGIPIRTLRNISLNPNFGGQAMVVSLGCEKLQPNRLLPENMIPIHKEGEPYVV 240 VAIDAPNAGIPIRTLRNISLNPNFGGQAMVVSLGCEKLQPNRLLPENMIPIHK+GEPYVV Sbjct: 181 VAIDAPNAGIPIRTLRNISLNPNFGGQAMVVSLGCEKLQPNRLLPENMIPIHKQGEPYVV 240 Query: 241 CLQDAQHVGFNSMIDSIMNMAEARLTELNKRRRETCPASDLVVGVQCGGSDAFSGVTANP 300 CLQDA+HVGFNSMIDSIMNMAEARLTELNKRRRETCPASDLVVGVQCGGSDAFSGVTANP Sbjct: 241 CLQDAEHVGFNSMIDSIMNMAEARLTELNKRRRETCPASDLVVGVQCGGSDAFSGVTANP 300 Query: 301 AVGYATDLLVRAGASVMFSEVTEVRDGIDQLTSRAINEEVAQAMIREMDWYDNYLKQGGV 360 AVG+ATDLLVRAGASVMFSEVTEVRDGIDQLTSRA+NEEVAQAMIREMDWYDNYLKQGGV Sbjct: 301 AVGFATDLLVRAGASVMFSEVTEVRDGIDQLTSRAVNEEVAQAMIREMDWYDNYLKQGGV 360 Query: 361 DRSANTTPGNKKGGLANIVEKAMGSIVKSGSSPISGVLAPGDKLQQKGLIYAATPASDFI 420 DRSANTTPGNKKGGLANIVEKAMGSIVKSGSSPISGVL+PGDKLQQKGLIYAATPASDFI Sbjct: 361 DRSANTTPGNKKGGLANIVEKAMGSIVKSGSSPISGVLSPGDKLQQKGLIYAATPASDFI 420 Query: 421 CGTLQLAAGMNLHIFTTGRGTPYGLAAVPVIKVATRNDLARRWHDLMDVNAGRIASGEAT 480 CGTLQLAAGMNLHIFTTGRGTPYGLAAVPVIKVATRNDLARRWHDLMDVNAGRIASGEA+ Sbjct: 421 CGTLQLAAGMNLHIFTTGRGTPYGLAAVPVIKVATRNDLARRWHDLMDVNAGRIASGEAS 480 Query: 481 IEQVGWELFQLMLDVASGRKRTWAEQWKLHNALTLFNPAPVT 522 IE VGWELFQLMLDVASG+KRTWAEQWKLHNALTLFNPAPVT Sbjct: 481 IEDVGWELFQLMLDVASGKKRTWAEQWKLHNALTLFNPAPVT 522 >gi|300313170|ref|YP_003777262.1| altronate hydrolase [Herbaspirillum seropedicae SmR1] Length = 511 Score = 279 bits (713), Expect = 4e-76, Method: Compositional matrix adjust. Identities = 182/509 (35%), Positives = 269/509 (52%), Gaps = 38/509 (7%) Query: 9 QAFTPRYIMMNDTDNVAIVVNDGGLPAGTVFPD--GLTLIDRVPQGHKIALRDLKQGDAI 66 +A +P I +N D+V I L +GTV D GL + +P GHK+A R + G+ + Sbjct: 2 KAHSP-VIRLNPVDDVVIARQQ--LISGTVLQDEGGLKVQGLIPAGHKMATRAITAGEPV 58 Query: 67 VRYDVAIGYAVRDIPKGSWIE-ESLVQMPPARE----LDNLPI-ATKKPAPQPPLEGYTF 120 RY IG A +DI G + +L +RE D P+ PA TF Sbjct: 59 KRYGQIIGTASQDIAPGQHVHTHNLAMAEFSREHHFGADVKPVDFVATPA--------TF 110 Query: 121 EGYRNADGSVGTRNLLAITTTVQCVA----GVVEHAVKRIRAELLPKYPNVEDVVALEHT 176 G DG V TRN + + T+V C A + ++ + I E+L YPN++ VVAL H Sbjct: 111 MGIVRPDGRVATRNYIGVLTSVNCSATAARAIADYFRRDIHPEVLADYPNIDGVVALTHG 170 Query: 177 YGCGVAIDAPNAGIPIRTLRNISLNPNFGGQAMVVSLGCEKLQPNRLLPENMIPIHKEGE 236 GC + I RTL + +PNF +VV LGCE Q + L+ + + KEGE Sbjct: 171 QGCALDSQGEPLQILRRTLAGYATHPNFAA-VLVVGLGCETNQISGLMESHNL---KEGE 226 Query: 237 PY-VVCLQ----DAQHVGFNSMIDSIMNMAEARLTELNKRRRETCPASDLVVGVQCGGSD 291 + +Q A+ V I+ I M L + N +RE A L +G+QCGGSD Sbjct: 227 YFHTFTIQGTGGTAKTVALG--IEKIKKM----LPKANDIKREPVSAKHLTLGLQCGGSD 280 Query: 292 AFSGVTANPAVGYATDLLVRAGASVMFSEVTEVRDGIDQLTSRAINEEVAQAMIREMDWY 351 +SG+TANPA+G A DLLVR G + + SE E+ LT RA++ EV + ++ + W+ Sbjct: 281 GYSGITANPALGAAVDLLVRHGGTAILSETPEIYGAEHLLTRRAVSPEVGEKLLARIAWW 340 Query: 352 DNYLKQGGVDRSANTTPGNKKGGLANIVEKAMGSIVKSGSSPISGVLAPGDKLQQKGLIY 411 + Y + + + N + GNK GGL I+EK++G++ K G++ + V + + +G ++ Sbjct: 341 EEYCAKNDAEMNNNPSAGNKAGGLTTILEKSLGAVAKGGTTNLVDVYKYAEAVTARGFVF 400 Query: 412 AATPASDFICGTLQLAAGMNLHIFTTGRGTPYGLAAVPVIKVATRNDLARRWHDLMDVNA 471 TP D I T Q+A G N+ FTTGRG+ YG A P +K+AT L +R + MD+N Sbjct: 401 MDTPGYDPISATGQVAGGANMICFTTGRGSAYGCAPAPSLKLATNTALWQRQEEDMDINC 460 Query: 472 GRIASGEATIEQVGWELFQLMLDVASGRK 500 G IA G T +++G FQ++LD ASG+K Sbjct: 461 GEIADGNVTPQEIGERFFQMILDTASGKK 489 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.135 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,753,568 Number of extensions: 78623 Number of successful extensions: 215 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 211 Number of HSP's successfully gapped: 2 Length of query: 522 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 432 Effective length of database: 1,193,720 Effective search space: 515687040 Effective search space used: 515687040 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)