BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3082.p (149 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312418|ref|YP_003776510.1| signal peptide protein [Herbasp... 223 4e-60 gi|300312282|ref|YP_003776374.1| hypothetical protein Hsero_2978... 78 4e-16 >gi|300312418|ref|YP_003776510.1| signal peptide protein [Herbaspirillum seropedicae SmR1] Length = 143 Score = 223 bits (569), Expect = 4e-60, Method: Compositional matrix adjust. Identities = 105/114 (92%), Positives = 110/114 (96%) Query: 36 SSTPVPVTETKPVLPQLLQAPAATQPTWATQLKTGAFSCEMGNKVEIRMDGRVTDGITLV 95 S VPVTETKPVLPQLLQ+PAATQPTWATQLKTGAFSCEMGNKVE+RMDGRVTDG+TLV Sbjct: 30 SREEVPVTETKPVLPQLLQSPAATQPTWATQLKTGAFSCEMGNKVELRMDGRVTDGVTLV 89 Query: 96 WKGSSYMMNPVSTSTGAVRLENKGEGLVWIQIPSKSMLLNSKIGQQLANDCRTR 149 WKG +YMMNPVSTSTGAVRLENKGEGLVWIQIPSKSMLLN+KIGQQLANDCRTR Sbjct: 90 WKGKNYMMNPVSTSTGAVRLENKGEGLVWIQIPSKSMLLNAKIGQQLANDCRTR 143 >gi|300312282|ref|YP_003776374.1| hypothetical protein Hsero_2978 [Herbaspirillum seropedicae SmR1] Length = 182 Score = 77.8 bits (190), Expect = 4e-16, Method: Compositional matrix adjust. Identities = 36/77 (46%), Positives = 49/77 (63%) Query: 72 FSCEMGNKVEIRMDGRVTDGITLVWKGSSYMMNPVSTSTGAVRLENKGEGLVWIQIPSKS 131 F CEMG+K+ I + I + W + + V T+TGA R EN+ GLVWI IP+K Sbjct: 81 FQCEMGHKLTIWKNETDDKHIAIRWDKRIHRLTRVETTTGANRFENRKMGLVWIGIPAKG 140 Query: 132 MLLNSKIGQQLANDCRT 148 +LL+SK GQQLAN+C+ Sbjct: 141 LLLDSKKGQQLANECKN 157 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.129 0.383 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 364,061 Number of extensions: 11313 Number of successful extensions: 63 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 63 Number of HSP's successfully gapped: 2 Length of query: 149 Length of database: 1,619,870 Length adjustment: 77 Effective length of query: 72 Effective length of database: 1,255,275 Effective search space: 90379800 Effective search space used: 90379800 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 126 (53.1 bits)