BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3081.p (628 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312417|ref|YP_003776509.1| propionyl-CoA ligase [Herbaspir... 1182 0.0 gi|300310875|ref|YP_003774967.1| acetyl-coenzyme A synthetase [H... 421 e-119 gi|300309365|ref|YP_003773457.1| acetyl-coenzyme A synthetase [H... 170 3e-43 gi|300310257|ref|YP_003774349.1| acetyl-coenzyme A synthetase [H... 164 2e-41 gi|300313115|ref|YP_003777207.1| long-chain-fatty-acid-CoA ligas... 137 3e-33 >gi|300312417|ref|YP_003776509.1| propionyl-CoA ligase [Herbaspirillum seropedicae SmR1] Length = 628 Score = 1182 bits (3057), Expect = 0.0, Method: Compositional matrix adjust. Identities = 573/628 (91%), Positives = 593/628 (94%) Query: 1 MQYEDFYRRSIEHPDTYWAEEAQRIHWETPFTRTLDYSRAPFARWFIGGTTNLCYNAVDR 60 MQYE FYR+S+E P+ +WA+EAQRIHW+ PF+RTLDYSR PFARWFI G TNLC+NAVDR Sbjct: 1 MQYEQFYRQSVEQPEAFWAQEAQRIHWQQPFSRTLDYSRPPFARWFIDGQTNLCHNAVDR 60 Query: 61 HLAERPTQAALIAISTETNSERVYDFAELHREVQAMAATMLALGVQRGDRVLIYMPMIAE 120 HL ER QAALIAISTETNSERVY +AEL +EV AMAA+M ALGVQRGDRVLIYMPMIAE Sbjct: 61 HLPERAQQAALIAISTETNSERVYTYAELQQEVMAMAASMQALGVQRGDRVLIYMPMIAE 120 Query: 121 AVFAMLACARIGAVHSVVFGGFASNSLASRIDDAQPCLIVSADAGSRGGKVIPYKGLLDE 180 AVFAMLACARIGAVHSVVFGGFASNSLASRIDDAQP LIVSADAGSRGGKVIPYKGLLDE Sbjct: 121 AVFAMLACARIGAVHSVVFGGFASNSLASRIDDAQPRLIVSADAGSRGGKVIPYKGLLDE 180 Query: 181 AINLAQHKPQRVLLVDRGLAEMARTPQRDVDYAQLRQQHLDQPVPVTWLESNDTSYVLYT 240 AI +AQH+PQ VLLVDRGLA MART QRDVDYA LR QHL Q VPVTWLESN +SY+LYT Sbjct: 181 AIAMAQHRPQHVLLVDRGLAPMARTAQRDVDYAPLRTQHLGQQVPVTWLESNASSYILYT 240 Query: 241 SGTTGKPKGVQRDVGGYAVALASSMQHIFCGQPGQTYFCTSDIGWVVGHSYIVYGPLIAG 300 SGTTGKPKGVQRDVGGYAVALASSMQHIFCGQPG+TYFCTSDIGWVVGHSYIVYGPLIAG Sbjct: 241 SGTTGKPKGVQRDVGGYAVALASSMQHIFCGQPGETYFCTSDIGWVVGHSYIVYGPLIAG 300 Query: 301 MATVLYEGLPIRPDAGIWWSIVEKYKVSRMFSAPTAIRVLKKQPPECMERYDTSSLKALY 360 MATVLYEGLPIRPDAGIWWSIVEKYKV+RMFSAPTAIRVLKKQPPECMERYDTSSLKALY Sbjct: 301 MATVLYEGLPIRPDAGIWWSIVEKYKVTRMFSAPTAIRVLKKQPPECMERYDTSSLKALY 360 Query: 361 LAGEPLDETTSSWISGALKVPVIDNYWQTESGWPIISIAKGISDKPTRLGSPGVPMPGYR 420 LAGEPLDETTSSWISGALKVPVIDNYWQTESGWPIISIAKGI DKPTRLGSPGVPMPGYR Sbjct: 361 LAGEPLDETTSSWISGALKVPVIDNYWQTESGWPIISIAKGIDDKPTRLGSPGVPMPGYR 420 Query: 421 LAILDEATGEPCAAGEKGVVAIEGPLPPGCMQTVYGDDARFVNTYWRSLPREVYSTFDWG 480 LAILDEATGEPC +KGVVAIEGPLPPGCMQTVYGDD RFVNTYWRSLPREVYSTFDWG Sbjct: 421 LAILDEATGEPCGPDQKGVVAIEGPLPPGCMQTVYGDDERFVNTYWRSLPREVYSTFDWG 480 Query: 481 IRDADGYYFILGRTDDVINVAGHRLGTREIEESISSHPNVSEVAVVGVEDKLKGQVAFAF 540 IRD DGYYFILGRTDDVINVAGHRLGTREIEESISSHPNVSEVAVVGVEDKLKGQVAFAF Sbjct: 481 IRDKDGYYFILGRTDDVINVAGHRLGTREIEESISSHPNVSEVAVVGVEDKLKGQVAFAF 540 Query: 541 AIPKQADAIATPEQRKALEAEIMGVVDKQIGAVGRPARVFFVSGLPKTRSGKLLRRAIQS 600 AIPKQA IATPEQRKALEAEIM VVDKQIGAVGRPARVFFVSGLPKTRSGKLLRRAIQS Sbjct: 541 AIPKQAYTIATPEQRKALEAEIMAVVDKQIGAVGRPARVFFVSGLPKTRSGKLLRRAIQS 600 Query: 601 ICEGRDAGDLPTIEDPAALEQVRAALRD 628 ICEGRD GDLPTIEDPAALEQVRA ++D Sbjct: 601 ICEGRDPGDLPTIEDPAALEQVRAVIKD 628 >gi|300310875|ref|YP_003774967.1| acetyl-coenzyme A synthetase [Herbaspirillum seropedicae SmR1] Length = 660 Score = 421 bits (1082), Expect = e-119, Method: Compositional matrix adjust. Identities = 241/621 (38%), Positives = 367/621 (59%), Gaps = 21/621 (3%) Query: 18 WAEEAQ-RIHWETPFTRTLDYSRAPFARWFIGGTTNLCYNAVDRHLAE-RPTQAALIAIS 75 WA A+ + W PFT+TLD S APF +WF G N+ YN +D +L + A+I S Sbjct: 47 WARLAKDNLKWHKPFTQTLDESNAPFYKWFADGQVNVSYNCLDVNLENGNADKTAVIFES 106 Query: 76 TETNSERVYDFAELHREVQAMAATMLALGVQRGDRVLIYMPMIAEAVFAMLACARIGAVH 135 + RV + ELH++V A + +LG+++GDRV+IYMPM E V AM ACARIGA H Sbjct: 107 DDGKVTRV-SYRELHQKVCQFANGLKSLGIKKGDRVVIYMPMSVEGVAAMQACARIGATH 165 Query: 136 SVVFGGFASNSLASRIDDAQPCLIVSADAGSRGGKVIPYKGLLDEAINLAQHKP-QRVLL 194 SVVFGGF++ SL R+ DA +++AD RGGK +P K ++DEA+++ + V++ Sbjct: 166 SVVFGGFSAKSLHERVIDAGAVAVLTADYQVRGGKQLPLKSIVDEALDMGGCDTLKHVVV 225 Query: 195 VDRGLAEMARTPQRDVDYAQLRQQHLDQPVPVTWLESNDTSYVLYTSGTTGKPKGVQRDV 254 R AE+ RD+ + + D P W+++ ++LYTSG+TGKPKGVQ Sbjct: 226 YKRAGAEINWVEGRDLWLSDVVANQSDTCEP-EWVDAEHPLFILYTSGSTGKPKGVQHAS 284 Query: 255 GGYAVALASSMQHIFCGQPGQTYFCTSDIGWVVGHSYIVYGPLIAGMATVLYEGLPIRPD 314 GY + +M+ F +P Y+CT+DIGW+ GH+YI YGPL G +++EG+P P+ Sbjct: 285 AGYLLWAVLTMKWTFDIKPDDVYWCTADIGWITGHTYIAYGPLAVGATQIVFEGVPTYPN 344 Query: 315 AGIWWSIVEKYKVSRMFSAPTAIRVLKKQPPECM----ERYDTSSLKALYLAGEPLDETT 370 AG +W +V ++K + ++APTAIR L K ++YD SSL+ L GEP++ Sbjct: 345 AGRFWDMVARHKATIFYTAPTAIRSLIKAADADEKVHPKQYDLSSLRILGSVGEPINPEA 404 Query: 371 SSWI---SGALKVPVIDNYWQTESGWPIISIAKGISDKPTRLGSPGVPMPGYRLAILDEA 427 W G P++D +WQTE+G +IS G + P GS +P+PG AI+DE Sbjct: 405 WMWYYKNVGHENCPIVDTFWQTETGGHMISPLPGAT--PQVPGSCTLPLPGITAAIVDE- 461 Query: 428 TGEPCAAGEKGVVAIEGPLPPGCMQTVYGDDARFVNTYW-RSLPREVYSTFDWGIRDAD- 485 TG G G++ ++ P P ++T++ D RF +Y+ L ++Y D +R+ + Sbjct: 462 TGNDIPNGNGGILVVKRPW-PSMIRTIWNDPERFKKSYFPEELGGKIYLAGDGAVRNKET 520 Query: 486 GYYFILGRTDDVINVAGHRLGTREIEESISSHPNVSEVAVVGVEDKLKGQVAFAFAIPKQ 545 GY+ I GR DDV+NV+GHR+GT EIE ++ ++ V+E AVVG D+ G+ AF + K+ Sbjct: 521 GYFTITGRIDDVLNVSGHRMGTMEIESALVANSIVAEAAVVGKPDETTGESICAFVVLKR 580 Query: 546 ADAIATPEQRKALEAEIMGVVDKQIGAVGRPARVFFVSGLPKTRSGKLLRRAIQSICEGR 605 T ++ K + E+ V K+IG + +P + F LPKTRSGK++RR ++ + +G Sbjct: 581 PR--PTGDEAKQIAKELRDWVAKEIGPIAKPKEIRFGDNLPKTRSGKIMRRLLRVLAKGE 638 Query: 606 D-AGDLPTIEDPAALEQVRAA 625 D+ T+E+PA L+Q++ A Sbjct: 639 AITQDVSTLENPAILDQLKEA 659 >gi|300309365|ref|YP_003773457.1| acetyl-coenzyme A synthetase [Herbaspirillum seropedicae SmR1] Length = 549 Score = 170 bits (430), Expect = 3e-43, Method: Compositional matrix adjust. Identities = 164/568 (28%), Positives = 263/568 (46%), Gaps = 57/568 (10%) Query: 45 WFIGGTTNLCYNAVDRHLAERPTQAALIAISTETNSERVYDFAELHREVQAMAATMLALG 104 W I N+ A DR A+ + A+I + S Y + +L A + A G Sbjct: 26 WQIPTHFNIAQAACDR-WADGSGRTAIICEDAD-GSVTSYSYDQLKILSDRFANALRAAG 83 Query: 105 VQRGDRVLIYMPMIAEAVFAMLACARIGAVHSVVFGGFASNSLASRIDDAQPCLIVSADA 164 V RGDR+ IY+ E V LA ++GA+ +F F +++A R+D++ +V+ + Sbjct: 84 VGRGDRIGIYLSQRIETVITHLAAYKLGAITVPLFYLFGPDAIAYRLDNSGAVALVTDAS 143 Query: 165 GSRGGKVIPYKGLLDEAINLAQHKPQRVLLVDRGLAEMARTPQRDV-DYAQLRQQHLDQP 223 G E LA P L+ +++ D D LD Sbjct: 144 GM-------------EKATLAGELPALRLVFCVESSDLVLPQTTDFWDRLHAAPAELD-- 188 Query: 224 VPVTWLESNDTSYVLYTSGTTGKPKGV---QRDVGGYAVALASSMQHIFCGQPGQTYFCT 280 PV L ++D + ++YTSGTTGK KG R + G+ + S H Q G ++ Sbjct: 189 -PVLTL-ADDPAMIIYTSGTTGKAKGALHAHRVLLGHLPGVEVS--HDSFPQEGDRFWTP 244 Query: 281 SDIGWVVGHSYIVYGPLIAGMATVLYEGLPIRPDAGIWWSIVEKYKVSRMFSAPTAIRVL 340 +D W+ G ++ L G+A V + DA + ++ ++++ +F PTA+++L Sbjct: 245 ADWAWIGGLLDVLLPSLYHGVAVVARR--LEKFDAAEVFGLLARHQIRNVFFPPTALKML 302 Query: 341 KKQPPECMERYDTSSLKALYLAGEPLDETTSSWISGALKVPVIDNYWQTESGWPIISIAK 400 + + SL+++ GE L + +W AL V + + Y QTE + ++ Sbjct: 303 RGA--ATVRAQADFSLRSVASGGETLGDDLIAWGREALGVTINEFYGQTECN---LVVSS 357 Query: 401 GISDKPTRLGSPGVPMPGYRLAILDEATGEPCAAGEKGVVAIEGPLPPGCMQTVYGDDAR 460 P+ GS G +PG+ + I+DE G+ G G +AI P P ++ Sbjct: 358 SSHCYPSVSGSMGRAVPGHVVQIVDE-QGQVLPHGTVGNIAIRAPDPVMFLR-------- 408 Query: 461 FVNTYWRS--LPREVYS-----TFDWGIRDADGYYFILGRTDDVINVAGHRLGTREIEES 513 YWR+ RE ++ T D G D GY LGR DDVI AG+R+G IEE Sbjct: 409 ----YWRNEEATREKFAGDFLLTGDLGSMDEQGYIRYLGRNDDVITSAGYRIGPAAIEEC 464 Query: 514 ISSHPNVSEVAVVGVEDKLKGQVAFAFAIPKQADAIATPEQRKALEAEIMGVVDKQIGAV 573 + HP V AVVGV+D L+ +V AF + K TP++ AL+AE+ V Q+ A Sbjct: 465 LMRHPAVRIAAVVGVKDALRTEVVKAFVVLKDG---VTPDE--ALKAELQQHVRAQLAAH 519 Query: 574 GRPARVFFVSGLPKTRSGKLLRRAIQSI 601 P + FV+ LP T +GK++R+ ++ + Sbjct: 520 EYPRLISFVAALPTTATGKIMRKTLKEM 547 >gi|300310257|ref|YP_003774349.1| acetyl-coenzyme A synthetase [Herbaspirillum seropedicae SmR1] Length = 556 Score = 164 bits (416), Expect = 2e-41, Method: Compositional matrix adjust. Identities = 153/563 (27%), Positives = 246/563 (43%), Gaps = 52/563 (9%) Query: 52 NLCYNAVDRHLAERPTQAALIAISTETNSERVYDFAELHREVQAMAATMLALGVQRGDRV 111 N C DRH+ +++ E + Y FA++ +A + G+Q G V Sbjct: 38 NACVECCDRHVKGDNIALEFVSLHGE---HQQYSFAQVRSMAARVANLLRQQGIQPGQIV 94 Query: 112 LIYMPMIAEAVFAMLACARIGAVHSVVFGGFASNSLASRIDDAQPCLIVSADAGSRGGKV 171 +P E + +L R GAV+ +F F ++ R+ + LIV+ A Sbjct: 95 AGMLPRTPELLATVLGTLRAGAVYQPLFTAFGPKAIEHRLALSGASLIVTNVAN------ 148 Query: 172 IPYKGLLDEAINLAQHKPQRVLLVDRGLAEMARTPQR--DVDYAQLRQQHLDQPVPVTWL 229 + LDE N Q R + P R D+D+ D PV Sbjct: 149 ---RDKLDEIANCPQVCTVR----------ESNDPLRAGDIDFRAAVDAQSDDFAPV-LR 194 Query: 230 ESNDTSYVLYTSGTTGKPKGVQRDVGGYAVALASSMQHIFCGQPGQTYFCTSDIGWVVGH 289 D ++ TSGTTG KGV + A + M+ P ++ +D GW G Sbjct: 195 SGADLMLMMSTSGTTGAAKGVPVPLSALQ-AFSVYMREAVGLLPQDKFWNMADPGWAYGL 253 Query: 290 SYIVYGPLIAGMATVLYEGLPIRPDAGIWWSIVEKYKVSRMFSAPTAIRVLKKQPPECME 349 Y + GPL G E + + + ++ + APTA R++ E Sbjct: 254 YYAIIGPLAIGHGITFNEAAFTVSGT---YETIRRLGITSLAGAPTAYRMMMAAGEEAAA 310 Query: 350 RYDTSSLKALYLAGEPLDETTSSWISGALKVPVIDNYWQTESGWPIISIAKGISDKPTRL 409 L+A+ AGEPL+ W + AL VP+ D+Y QTE G +++ + + Sbjct: 311 SV-KGRLRAVSSAGEPLNAEVVRWFASALDVPIHDHYGQTELGM-VVNNHHALRHEVVP- 367 Query: 410 GSPGVPMPGYRLAILDEATGEPCAAGEKGVVAIEGPLPPGCMQTVYGDDARFVNTYWR-- 467 GS G MPGYR+ +LDE E A + G +AI+ P +Y + + YW+ Sbjct: 368 GSAGFAMPGYRVVVLDENHQE-LGANQPGELAIDIARSP-----LY-----WFSGYWKQD 416 Query: 468 --SLPREVYSTFDWGIRDADGYYFILGRTDDVINVAGHRLGTREIEESISSHPNVSEVAV 525 ++ YST D + +G +GR+DDVI AG+R+G ++E ++ HP V++VAV Sbjct: 417 TPAIAHGYYSTGDNVELEPNGSISFIGRSDDVITSAGYRIGPFDVESALLEHPAVADVAV 476 Query: 526 VGVEDKLKGQVAFAFAIPKQADAIATPEQRKALEAEIMGVVDKQIGAVGRPARVFFVSGL 585 +G+ D + ++ AF + +D + AL ++ V +++ A P + F+ L Sbjct: 477 IGLPDPERTEIVKAFVV--LSDQFKASD---ALREQLAQHVKRRLSAHAYPRAIEFLDAL 531 Query: 586 PKTRSGKLLRRAIQSICEGRDAG 608 PKT SGKL R ++ + R AG Sbjct: 532 PKTPSGKLQRFVLRKMEADRRAG 554 >gi|300313115|ref|YP_003777207.1| long-chain-fatty-acid-CoA ligase [Herbaspirillum seropedicae SmR1] Length = 556 Score = 137 bits (344), Expect = 3e-33, Method: Compositional matrix adjust. Identities = 139/570 (24%), Positives = 244/570 (42%), Gaps = 83/570 (14%) Query: 58 VDRHLAERPTQAALIAISTETNSERVYDFAELHREVQAMAATMLALGVQRGDRVLIYMPM 117 V R + P ++A S+R+ + E++ V A+A + GV++GD++L++ Sbjct: 39 VARRFPDEP--GLILAQGAADGSDRLIPWREINARVDALAHALTRRGVKKGDKMLVHSRN 96 Query: 118 IAEAVFAMLACARIGAVHSVVFGGFASNSLASRIDDAQPCLIVSADAGSRGGKVIPYKGL 177 + + A ++G V V F ++ P Sbjct: 97 NQQIFESAWAAFKMGVVW--VPTNF---------------------------RITP---- 123 Query: 178 LDEAINLAQHKPQRVLLVDRGLAE-----------------MARTPQRDVDYAQLRQQHL 220 EA L Q V+L DRG A+ +A ++DY L ++ Sbjct: 124 -PEAAYLGQSSGACVMLYDRGFAQYVDAVKAASPALEHVVALADARAGELDYEALVREGS 182 Query: 221 DQPVPVTWLESNDTSYVLYTSGTTGKPKGVQRDVGGYAVALASSMQHIFCGQPGQTYFCT 280 ++ +D + YTSGTTG PK G A + + + + PG T+ Sbjct: 183 GAAFREAEVDYDDPLWFFYTSGTTGHPKAGMLSHGQMAFVVTNHLADLM---PGLTHQSR 239 Query: 281 SDIGWVVGHSYIVYGPLIA--GMATVLYEGLPIRPDAGIWWSIVEKYKVSRMFSAPTAIR 338 S + + H V+ + G A++L + + W +VEKY+V +F+ PT ++ Sbjct: 240 SLVVAPLSHGAGVHAVINTARGAASILLSSERLVVEEA--WQLVEKYRVDNLFTVPTIVK 297 Query: 339 VLKKQPPECMERYDTSSLKALYLAGEPLDETTSSWISGALKVPVIDNYWQTESGWPIISI 398 +L + E ++RYD SSLK + AG P+ + L ++ Y E I + Sbjct: 298 ILVED--ESVDRYDHSSLKHVIYAGAPMYRADQVYALKKLGKVLVQYYGLGEVTGNITFL 355 Query: 399 AKGI-----SDKPTRLGSPGVPMPGYRLAILDEATGEPCAAGEKGVVAIEGPLPPGCMQT 453 + + R+G+ G+P G ++AIL++ G+ A E G + + GP Sbjct: 356 PPYLHFEDDAHPQARVGTCGMPRTGMQIAILND-DGQELAPFETGEICVRGP-------A 407 Query: 454 VYGDDARFVNTYWRSLPREVYSTFDWGIRDADGYYFILGRTDDVINVAGHRLGTREIEES 513 V+ ++ + + T D G D DG+ +I GR+ D+ G + REIEE+ Sbjct: 408 VFMGYHNNPEANAKAFKHDWFHTGDLGHVDQDGFLYITGRSSDMYISGGSNVYPREIEEA 467 Query: 514 ISSHPNVSEVAVVGVEDKLKGQVAFAFAIPKQADAIATPEQRKALEAEIMGVVDKQIGAV 573 + +HP VSEVAV+GV D G+ A + K + +AL A + + +IG Sbjct: 468 LLTHPAVSEVAVLGVPDPKWGESGLAVIVCKAGQQV----DGEALLAHL----ESRIGKY 519 Query: 574 GRPARVFFVSGLPKTRSGKLLRRAIQSICE 603 P R + +PK+ GK++++ I+++ E Sbjct: 520 KWPRRFIYWDSMPKSGYGKIVKKQIKALLE 549 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.136 0.414 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,158,499 Number of extensions: 98848 Number of successful extensions: 320 Number of sequences better than 1.0e-08: 9 Number of HSP's gapped: 283 Number of HSP's successfully gapped: 14 Length of query: 628 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 537 Effective length of database: 1,188,985 Effective search space: 638484945 Effective search space used: 638484945 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)