BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3043.p (482 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312381|ref|YP_003776473.1| glutamyl-tRNA synthetase [Herba... 978 0.0 gi|300312871|ref|YP_003776963.1| glutamyl/glutaminyl-tRNA synthe... 124 1e-29 gi|300312914|ref|YP_003777006.1| glutaminyl-tRNA synthetase [Her... 66 5e-12 >gi|300312381|ref|YP_003776473.1| glutamyl-tRNA synthetase [Herbaspirillum seropedicae SmR1] Length = 482 Score = 978 bits (2529), Expect = 0.0, Method: Compositional matrix adjust. Identities = 473/482 (98%), Positives = 477/482 (98%) Query: 1 MAGPGNPAPAWFLLAIMTATQVRTRFAPSPTGYLHLGGARTALFSWAYARRFGGTFVLRI 60 MAGPGNP PAWFLLAIMTATQVRTRFAPSPTGYLHLGGARTALFSWAYARRFGGTFVLRI Sbjct: 1 MAGPGNPGPAWFLLAIMTATQVRTRFAPSPTGYLHLGGARTALFSWAYARRFGGTFVLRI 60 Query: 61 EDTDVERSTPAAVQAIIEGMQWLGLEHDEGPFYQMKRMDRYREVIAQMLEQGTAYYCYCT 120 EDTDVERSTP AVQAIIEGMQWLGLEHDEGPFYQMKRMDRYREVIAQMLEQGTAYYCYCT Sbjct: 61 EDTDVERSTPEAVQAIIEGMQWLGLEHDEGPFYQMKRMDRYREVIAQMLEQGTAYYCYCT 120 Query: 121 PEEVEAMRERQRAAGEKPRYDGTWRPEPGKTLPAIPEGRKPVVRFKNPQDGEVSWNDVVK 180 PEEVEAMRERQRAAGEKPRYDGTWRPEPGKTLPAIPEGRKPVVRFKNPQDGEVSWNDVVK Sbjct: 121 PEEVEAMRERQRAAGEKPRYDGTWRPEPGKTLPAIPEGRKPVVRFKNPQDGEVSWNDVVK 180 Query: 181 GPITISNRELDDLVIARPDGTPTYNFCVVVDDWDMKITHVIRGDDHVNNTPRQINILKAL 240 GPITISNRELDDLVIARPDGTPTYNFCVVVDDWDMKITHVIRGDDHVNNTPRQINILKAL Sbjct: 181 GPITISNRELDDLVIARPDGTPTYNFCVVVDDWDMKITHVIRGDDHVNNTPRQINILKAL 240 Query: 241 GGTLPEYGHVPMILGADGEKLSKRHGAVSVMDYPAQGYLPEAMLNYLARLGWSHGDDEIF 300 GGTLPEYGHVPMILGADGEKLSKRHGAVSVMDYPAQGYLPEAMLNYLARLGWSHGDDEIF Sbjct: 241 GGTLPEYGHVPMILGADGEKLSKRHGAVSVMDYPAQGYLPEAMLNYLARLGWSHGDDEIF 300 Query: 301 SMQQFCEWFDLDHLTKAPAQFNPEKLNWLNNHYIKLADNARLAGLVRPLLEAQGAVFDGA 360 SMQQFC WFDLDHLTKAPAQFNPEKLNWLNNHYIKLADNARLAGLVRPLLEAQGAVF+GA Sbjct: 301 SMQQFCAWFDLDHLTKAPAQFNPEKLNWLNNHYIKLADNARLAGLVRPLLEAQGAVFEGA 360 Query: 361 PDLAAVIGLLKDRANTVNEIAAAALMFYRQPAPDAALVAQHITDAIKPALADFAQRVATV 420 PDLAAVI LLKDRANTVNEIAAAALMFYRQPAPDAALVAQHIT+AIKPALADFAQRVATV Sbjct: 361 PDLAAVIALLKDRANTVNEIAAAALMFYRQPAPDAALVAQHITEAIKPALADFAQRVATV 420 Query: 421 EWTKEAIAPMIKEVLAAHGIKMPQLAMPLRLIVAGQLQTPAIDAVLQLFGREAVKARLAA 480 EWTKEAIAPMIKEVLAAHGIKMPQLAMPLRLIVAGQLQTPAIDAVLQLFGREAV+ARL+ Sbjct: 421 EWTKEAIAPMIKEVLAAHGIKMPQLAMPLRLIVAGQLQTPAIDAVLQLFGREAVQARLSG 480 Query: 481 YL 482 YL Sbjct: 481 YL 482 >gi|300312871|ref|YP_003776963.1| glutamyl/glutaminyl-tRNA synthetase [Herbaspirillum seropedicae SmR1] Length = 294 Score = 124 bits (312), Expect = 1e-29, Method: Compositional matrix adjust. Identities = 94/258 (36%), Positives = 132/258 (51%), Gaps = 15/258 (5%) Query: 25 RFAPSPTGYLHLGGARTALFSWAYARRFGGTFVLRIEDTDVERSTPAAVQAIIEGMQWLG 84 RFAPSP+G LH G AL S+ AR G ++LRIED D R+ A Q II + L Sbjct: 9 RFAPSPSGPLHAGSLVAALASYLDARVHAGRWLLRIEDIDETRTVQGAAQDIIATLAALD 68 Query: 85 LEHDEGPFYQMKRMDRYREVIAQMLEQGTAYYCYCTPEEVEAMRERQRAAGEKPRYDGTW 144 + D Q +R RY+ +A+ AY C C+ +E+ R + G Y GT Sbjct: 69 MRSDGPVLVQSQRKARYQ--LARDRLGALAYPCGCSRKEIADSRVGTASDGAA-LYPGTC 125 Query: 145 RP--EPGKTLPAIPEGRKPVVRFKNP-QDGE-VSWNDVVKGPITIS-NRELDDLVIARPD 199 R PGK + R +R +P Q GE V + D GP E+ D V+ R D Sbjct: 126 RHGLAPGK------QARTLRLRVPDPGQAGELVQFEDRWLGPQAQHLAAEVGDFVLQRAD 179 Query: 200 GTPTYNFCVVVDDWDMKITHVIRGDDHVNNTPRQINILKALGGTLPEYGHVPMILGADGE 259 G Y VVVDD + +T ++RG D +++T RQI + LG P Y HVP+++ GE Sbjct: 180 GFWAYQLAVVVDDAEQGVTDIVRGADLLDSTARQIYLQGLLGYPTPRYLHVPLLMNETGE 239 Query: 260 KLSKRHGAVSV-MDYPAQ 276 K SK++GA ++ ++ P Q Sbjct: 240 KFSKQNGAQALDLNQPLQ 257 >gi|300312914|ref|YP_003777006.1| glutaminyl-tRNA synthetase [Herbaspirillum seropedicae SmR1] Length = 583 Score = 66.2 bits (160), Expect = 5e-12, Method: Compositional matrix adjust. Identities = 42/135 (31%), Positives = 64/135 (47%), Gaps = 10/135 (7%) Query: 21 QVRTRFAPSPTGYLHLGGARTALFSWAYARRFGGTFVLRIEDTDVERSTPAAVQAIIEGM 80 QV TRF P P GYLH+G A++ ++ A + G +R +DT+ E+ V I++ + Sbjct: 44 QVVTRFPPEPNGYLHIGHAKSICLNFGLAADYAGRCHMRFDDTNPEKEEQEYVDTILDSV 103 Query: 81 QWLGL--EHDEGP---FYQMKRMDRYREVIAQMLEQGTAYYCYCTPEEVEAMRERQRAAG 135 +WLG EH EG +Y D ++ +G A Y + E MRE R Sbjct: 104 KWLGFSWEH-EGKSHLYYASNYFDVMYACAQALIRKGLA---YVDEQNAEQMRE-NRGTL 158 Query: 136 EKPRYDGTWRPEPGK 150 +P + WR P + Sbjct: 159 TEPGKNSPWRDRPAE 173 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.137 0.423 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,748,822 Number of extensions: 80790 Number of successful extensions: 229 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 228 Number of HSP's successfully gapped: 3 Length of query: 482 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 393 Effective length of database: 1,198,455 Effective search space: 470992815 Effective search space used: 470992815 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)