BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3042.p (270 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312380|ref|YP_003776472.1| enoyl-CoA hydratase/carnithine ... 508 e-145 gi|300313068|ref|YP_003777160.1| enoyl-CoA hydratase [Herbaspiri... 123 1e-29 gi|300311864|ref|YP_003775956.1| enoyl-CoA hydratase [Herbaspiri... 110 1e-25 gi|300310158|ref|YP_003774250.1| enoyl-CoA hydratase [Herbaspiri... 100 2e-22 gi|300313417|ref|YP_003777509.1| enoyl-CoA hydratase II protein ... 95 7e-21 >gi|300312380|ref|YP_003776472.1| enoyl-CoA hydratase/carnithine racemase [Herbaspirillum seropedicae SmR1] Length = 269 Score = 508 bits (1308), Expect = e-145, Method: Compositional matrix adjust. Identities = 252/268 (94%), Positives = 259/268 (96%) Query: 3 SAAAHLDIDPRGLATITLNRPDIHNAFDDQLIAHLIDLITQAGDDARTCVLLLASTGKSF 62 S+AAHLDI PRGLATITLNRP+IHNAFDDQLIAHLIDL+TQA DD RTC+LLLASTGKSF Sbjct: 2 SSAAHLDITPRGLATITLNRPEIHNAFDDQLIAHLIDLLTQARDDTRTCLLLLASTGKSF 61 Query: 63 SAGADLAWMRRMADASREDNLRDARKLALLMHTLNSFPAPTLAKVQGAVMGGGVGLVSCC 122 SAGADLAWMRRMADASREDNLRDARKLALLM TLNSFP PTLAKVQGAVMGGGVGLVSCC Sbjct: 62 SAGADLAWMRRMADASREDNLRDARKLALLMETLNSFPTPTLAKVQGAVMGGGVGLVSCC 121 Query: 123 DIVVASEAAFFALSEVKLGLAPAAISPYVIAKMGVSQARRYFVSAERFPAACAAAMGLVH 182 DIVVAS+AAFFALSEVKLGLAPAAISPYVIAKMGVSQARRYFVSAERFPAA AAAMGLVH Sbjct: 122 DIVVASDAAFFALSEVKLGLAPAAISPYVIAKMGVSQARRYFVSAERFPAARAAAMGLVH 181 Query: 183 EVVPPGELDARTAQLIDTLLANGPQAMRAAKQLVQVANPLQVTPELSEYTAGVIADLRAS 242 EV PP ELDARTAQLIDTLLANGPQAMRAAKQLVQVANPLQVTPELSEYTAGVIADLRAS Sbjct: 182 EVAPPDELDARTAQLIDTLLANGPQAMRAAKQLVQVANPLQVTPELSEYTAGVIADLRAS 241 Query: 243 AEGREGLRAFLDKDAPAWTHKQPTAGAA 270 AEGREGLRAFLDKDAPAWT +QPT+GAA Sbjct: 242 AEGREGLRAFLDKDAPAWTRQQPTSGAA 269 >gi|300313068|ref|YP_003777160.1| enoyl-CoA hydratase [Herbaspirillum seropedicae SmR1] Length = 263 Score = 123 bits (309), Expect = 1e-29, Method: Compositional matrix adjust. Identities = 81/258 (31%), Positives = 132/258 (51%), Gaps = 7/258 (2%) Query: 7 HLDI----DPRGLATITLNRPDIHNAFDDQLIAHLIDLITQAGDDARTCVLLLASTGKSF 62 H DI D +G+ T+TLNRP NA ++++ L + D+ ++LA+ GK+F Sbjct: 5 HPDILEREDQQGITTLTLNRPQHFNALSEEMLTALQAQLDDIKSDSTVRCVILAANGKAF 64 Query: 63 SAGADLAWMRRMADASREDNLRDARKLALLMHTLNSFPAPTLAKVQGAVMGGGVGLVSCC 122 AG DL M+ + + L + + LM +L P P +AKVQG G LV+ C Sbjct: 65 CAGHDLRQMQATPQQTYYEAL--FHRCSHLMQSLRELPVPVIAKVQGLATAAGCQLVATC 122 Query: 123 DIVVASEAAFFALSEVKLGLAPAAISPYVIAKMGVSQARRYFVSAERFPAACAAAMGLVH 182 D+ +A+E+A FA+S + +GL + + + + + +A V+ + AA AAA GL++ Sbjct: 123 DLAIAAESARFAVSGINVGLFCSTPAVALTRNLPIKRAFEMLVTGKFIDAATAAAWGLIN 182 Query: 183 EVVPPGELDARTAQLIDTLLANGPQAMRAAKQLVQVANPLQVTPELSEYTAGVIADLRAS 242 + VP ELD+ T L + + ++ K +V + P+ + A V+A Sbjct: 183 QAVPEQELDSATQALAREICSKPAISIARGKAMV-YRQQMMALPDAYAHAAEVMACNIMD 241 Query: 243 AEGREGLRAFLDKDAPAW 260 E +EG+ AFLDK P W Sbjct: 242 PEAQEGITAFLDKRKPNW 259 >gi|300311864|ref|YP_003775956.1| enoyl-CoA hydratase [Herbaspirillum seropedicae SmR1] Length = 260 Score = 110 bits (275), Expect = 1e-25, Method: Compositional matrix adjust. Identities = 86/265 (32%), Positives = 129/265 (48%), Gaps = 18/265 (6%) Query: 7 HLDIDPRG-LATITLNRPDIHNAFDDQLIAHLIDLITQAGDDARTCVLLLASTG-KSFSA 64 +L ++ G A +TL+ P N + +A L L+ + D L++ G K FSA Sbjct: 6 NLKLEKTGHTAVLTLSNPPA-NTWTRAALADLTRLVKELNADRDIYTLVVTGEGEKFFSA 64 Query: 65 GADLAWMRRMADASREDNLRDARKLALLMHTLNSFPAPTLAKVQGAVMGGGVGLVSCCDI 124 GADL + AD + AR+ TL++F ++A + G MGGG+ CDI Sbjct: 65 GADL---KLFADGDKAMAREMARRFGEAFETLSAFRGVSIAAINGYAMGGGLECALACDI 121 Query: 125 VVASEAAFFALSEVKLGLAPAAIS----PYVIAKMGVSQARRYFVSAERFPAACAAAMGL 180 +A E A AL E +GL P A P+++ G A+R + ER A A +GL Sbjct: 122 RIAEEQAQMALPEAAVGLLPCAGGTQNLPWLV---GEGWAKRMILCGERVNAETALRIGL 178 Query: 181 VHEVVPPGELDARTAQLIDTLLANGPQAMRAAKQLVQVA--NPLQVTPELSEYTAGVIAD 238 V EVVP G+ R L P ++ A+KQL+Q A NPL + + D Sbjct: 179 VEEVVPRGQARERALALAQQAEKQSPSSVAASKQLIQGARHNPLGTILQTERE---LFVD 235 Query: 239 LRASAEGREGLRAFLDKDAPAWTHK 263 L + + REG++AFL+K +P W ++ Sbjct: 236 LFDTQDQREGVQAFLEKRSPQWKNQ 260 >gi|300310158|ref|YP_003774250.1| enoyl-CoA hydratase [Herbaspirillum seropedicae SmR1] Length = 281 Score = 99.8 bits (247), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 84/248 (33%), Positives = 127/248 (51%), Gaps = 3/248 (1%) Query: 14 GLAT---ITLNRPDIHNAFDDQLIAHLIDLITQAGDDARTCVLLLASTGKSFSAGADLAW 70 GLA+ I +NRP N D +I + D ++LA+ G+SFS G ++ W Sbjct: 19 GLASVGWIWINRPYDGNTIDRLTARDIIRAVGVFSKDPEVRAIVLAARGESFSHGIEVQW 78 Query: 71 MRRMADASREDNLRDARKLALLMHTLNSFPAPTLAKVQGAVMGGGVGLVSCCDIVVASEA 130 +R ADA L D RKLA L+ L PT+A+VQG G G GL + CDI +A++ Sbjct: 79 LRAQADAPINGALDDTRKLAQLLQVLAECEKPTVARVQGKAAGIGAGLAAACDICIAADH 138 Query: 131 AFFALSEVKLGLAPAAISPYVIAKMGVSQARRYFVSAERFPAACAAAMGLVHEVVPPGEL 190 A F + + +LG+APA I+P+++ +G Q +RYF + E AA A +GL HE VP L Sbjct: 139 AHFTMGDDRLGMAPAVITPHLVRAIGARQCQRYFQTNELLTAARACELGLAHEAVPVELL 198 Query: 191 DARTAQLIDTLLANGPQAMRAAKQLVQVANPLQVTPELSEYTAGVIADLRASAEGREGLR 250 D + L+ +LL +A +L++ T ++S + +RA R L Sbjct: 199 DEKIGALLASLLLGDVHVQHSANKLIRAIAAAPPTVDMSVDAVRIGVRMRACPSVRARLG 258 Query: 251 AFLDKDAP 258 ++ AP Sbjct: 259 LHAERSAP 266 >gi|300313417|ref|YP_003777509.1| enoyl-CoA hydratase II protein [Herbaspirillum seropedicae SmR1] Length = 263 Score = 94.7 bits (234), Expect = 7e-21, Method: Compositional matrix adjust. Identities = 83/266 (31%), Positives = 122/266 (45%), Gaps = 6/266 (2%) Query: 1 MSSAAAHLDIDPRGLATITLNRPDIHNAFDDQLIAHLIDLITQAGDDARTCVLLLASTGK 60 MSS + L+ + G+A ITLNRPD N+F + L D I D VLLL G+ Sbjct: 1 MSSPSILLE-NRDGIAVITLNRPDKLNSFTVAMHLELRDAIATLQADPSVRVLLLTGAGR 59 Query: 61 SFSAGADLAWMRRMADASREDNLRDA--RKLALLMHTLNSFPAPTLAKVQGAVMGGGVGL 118 F AG DL R + +L ++ + L+ +L + P P + V G G G L Sbjct: 60 GFCAGQDLG-DRAVKPGDDGVDLGESIDKYYGPLVKSLRALPFPVICAVNGVAAGAGANL 118 Query: 119 VSCCDIVVASEAAFFALSEVKLGLAPAAISPYVIAKM-GVSQARRYFVSAERFPAACAAA 177 CDIV+A+ +A F KLGL P Y + ++ G ++A + E+ A A Sbjct: 119 PLACDIVLAARSASFVEVFCKLGLIPDTGGTYFLPRLVGTARAMGMALLGEKISAEQAER 178 Query: 178 MGLVHEVVPPGELDARTAQLIDTLLANGPQAMRAAKQLVQVANPLQVTPELSEYTAGVIA 237 GL+ + V +L A + A P AA + A+P PE + + Sbjct: 179 WGLIWKCVDDDQL-MEEAHAMAAHFARAPTKGLAATKATLYASPAHTLPEQLDLERDTMR 237 Query: 238 DLRASAEGREGLRAFLDKDAPAWTHK 263 L S + REG+ AFL+K AP +T + Sbjct: 238 ALGRSRDYREGVTAFLEKRAPQFTGQ 263 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.132 0.381 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 864,280 Number of extensions: 35481 Number of successful extensions: 173 Number of sequences better than 1.0e-08: 10 Number of HSP's gapped: 161 Number of HSP's successfully gapped: 10 Length of query: 270 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 186 Effective length of database: 1,222,130 Effective search space: 227316180 Effective search space used: 227316180 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)