BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3035.p (502 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312371|ref|YP_003776463.1| methylmalonate-semialdehyde deh... 1006 0.0 gi|300313930|ref|YP_003778022.1| methylmalonate-semialdehyde deh... 459 e-130 gi|300314075|ref|YP_003778167.1| methylmalonate-semialdehyde deh... 452 e-128 gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrog... 220 2e-58 gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semial... 199 5e-52 >gi|300312371|ref|YP_003776463.1| methylmalonate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 506 Score = 1006 bits (2601), Expect = 0.0, Method: Compositional matrix adjust. Identities = 486/493 (98%), Positives = 491/493 (99%) Query: 1 MSQANIPHVPLYLNGEKVQSTAKEWRDVLNPATQEVVARVPFATKEEVDRAVANAKDTFK 60 MSQANIP+VPLYLNGEKVQST+KEWRDVLNPATQEVVARVPFATKEEVDRAVANAK+TFK Sbjct: 1 MSQANIPNVPLYLNGEKVQSTSKEWRDVLNPATQEVVARVPFATKEEVDRAVANAKETFK 60 Query: 61 TWRNTSLAQRMRIMLKFQQLLRENIAPLAELITREHGKTLPDAEGEVMRGLEVVEHACSI 120 TWRNTSLAQRMRIMLKFQQLLRENIAPLAELITREHGKTLPDAEGEVMRGLEVVEHACSI Sbjct: 61 TWRNTSLAQRMRIMLKFQQLLRENIAPLAELITREHGKTLPDAEGEVMRGLEVVEHACSI 120 Query: 121 TSLQLGELAENVAGGVDVYTLYQPLGVGAGITAFNFPVMLPCFMFPIAVACGNTFVLKPS 180 TSLQLGELAENVAGGVDVYTLYQPLGVGAGITAFNFPVMLPCFMFPIAVACGNTFVLKPS Sbjct: 121 TSLQLGELAENVAGGVDVYTLYQPLGVGAGITAFNFPVMLPCFMFPIAVACGNTFVLKPS 180 Query: 181 EQDPTSSLFLVELANQAGLPPGVLNVVHGGPDVANMICDHPDIKAVSFIGSTHVGTHIYR 240 EQDPTSSLFLVELANQAGLPPGVLNVVHGGPDVANMICDHPDIKAVSFIGSTHVGTHIYR Sbjct: 181 EQDPTSSLFLVELANQAGLPPGVLNVVHGGPDVANMICDHPDIKAVSFIGSTHVGTHIYR 240 Query: 241 RASEAGKRAQCMMGAKNHCIVLPDAPKDQAINNLLGAAFGAAGQRCMANSVVVLVGQTRE 300 RASEAGKRAQCMMGAKNHCIVLPDAPKDQAINNLLGAAFGAAGQRCMANSVVVLVGQTRE Sbjct: 241 RASEAGKRAQCMMGAKNHCIVLPDAPKDQAINNLLGAAFGAAGQRCMANSVVVLVGQTRE 300 Query: 301 WIPEIVERSKTMKVGPGTDRKADVGPLVSKAAKERVERLIASGVEQGAHLLLDGRNCKVA 360 WIPEIVERSK MKVGPGTDRKADVGPLVSKAAKERVERLIASGVEQGA LLLDGRNCKVA Sbjct: 301 WIPEIVERSKAMKVGPGTDRKADVGPLVSKAAKERVERLIASGVEQGAKLLLDGRNCKVA 360 Query: 361 GSENGNFVGPTVFTGVKPDMDIYTQEIFGPAMCIVELETLDEAIAFINANPNGNGTSIFT 420 GSENGNFVGPTVFTGVKP+MDIYTQEIFGPAMCIVEL+TLDEAIAFINANPNGNGTSIFT Sbjct: 361 GSENGNFVGPTVFTGVKPEMDIYTQEIFGPAMCIVELDTLDEAIAFINANPNGNGTSIFT 420 Query: 421 SSGYAARKFQNEIDVGQVGINVPIPVPVAYFSFTGSRASKLGDLGPNGKQAVTFWTQTKT 480 SSGYAARKFQNEIDVGQVGINVPIPVPVAYFSFTGSRASKLGDLGPNGKQAVTFWTQTKT Sbjct: 421 SSGYAARKFQNEIDVGQVGINVPIPVPVAYFSFTGSRASKLGDLGPNGKQAVTFWTQTKT 480 Query: 481 VTARWYAPDEEAG 493 VTARWYAPDEEAG Sbjct: 481 VTARWYAPDEEAG 493 >gi|300313930|ref|YP_003778022.1| methylmalonate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 497 Score = 459 bits (1180), Expect = e-130, Method: Compositional matrix adjust. Identities = 220/475 (46%), Positives = 310/475 (65%), Gaps = 1/475 (0%) Query: 12 YLNGEKVQSTAKEWRDVLNPATQEVVARVPFATKEEVDRAVANAKDTFKTWRNTSLAQRM 71 Y+ G++V + + + DV NPA VA+V T +EVD AV F W NT R Sbjct: 7 YIGGQRVDTRSGRYADVFNPAQGVPVAQVALGTSDEVDAAVKAGVAAFPAWSNTPPLTRA 66 Query: 72 RIMLKFQQLLRENIAPLAELITREHGKTLPDAEGEVMRGLEVVEHACSITSLQLGELAEN 131 R++ +F L+++ A +I REHGKT DA+GEV RG+E+VE A I + GE + Sbjct: 67 RVLFRFLHLIQQRADDFARIIVREHGKTFSDAQGEVARGIEIVEFAAGIPQMLKGEYTDQ 126 Query: 132 VAGGVDVYTLYQPLGVGAGITAFNFPVMLPCFMFPIAVACGNTFVLKPSEQDPTSSLFLV 191 +A G+D +++ Q LGV AGIT FNFP M+P +MFPIA+ACGN FVLKPSE+DP++SL + Sbjct: 127 IARGIDAWSMRQALGVVAGITPFNFPAMVPMWMFPIAIACGNCFVLKPSERDPSASLLIA 186 Query: 192 ELANQAGLPPGVLNVVHGGPDVANMICDHPDIKAVSFIGSTHVGTHIYRRASEAGKRAQC 251 +L +AGLP GV NVV G + + DHP +KA+SF+GST + +IY R S GKR Q Sbjct: 187 DLLKEAGLPDGVFNVVQGDKVTVDALLDHPQVKAISFVGSTPIAEYIYARGSAKGKRVQA 246 Query: 252 MMGAKNHCIVLPDAPKDQAINNLLGAAFGAAGQRCMANSVVVLVGQT-REWIPEIVERSK 310 + GAKNH +V+PDA A++ L+GAA+G+AG+RCMA SV + VG E + + ER++ Sbjct: 247 LGGAKNHMVVMPDADMQLAVDALMGAAYGSAGERCMAISVALAVGSAGDELVAALAERAR 306 Query: 311 TMKVGPGTDRKADVGPLVSKAAKERVERLIASGVEQGAHLLLDGRNCKVAGSENGNFVGP 370 +K+ G A++GP+V+ AAKER+E LI GVE+GA L++DGR +V G E G FVG Sbjct: 307 KLKINEGMAAGAEMGPVVTAAAKERIEGLIGRGVEEGATLVVDGRGYQVPGFEKGFFVGG 366 Query: 371 TVFTGVKPDMDIYTQEIFGPAMCIVELETLDEAIAFINANPNGNGTSIFTSSGYAARKFQ 430 T+ V P+M +Y +EIFGP +C++ + A+ INA+ GNG +++T G AR+F Sbjct: 367 TLLDHVTPEMTVYKEEIFGPVLCVLRCPDIASAVELINAHEYGNGVAVYTRDGGVAREFV 426 Query: 431 NEIDVGQVGINVPIPVPVAYFSFTGSRASKLGDLGPNGKQAVTFWTQTKTVTARW 485 +I+VG VG+NVP+PVP+A+ SF G + S GD G + V F+T+ K V RW Sbjct: 427 RQIEVGMVGVNVPLPVPMAFSSFGGWKRSLFGDHHMYGPEGVRFYTRAKAVMQRW 481 >gi|300314075|ref|YP_003778167.1| methylmalonate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 503 Score = 452 bits (1163), Expect = e-128, Method: Compositional matrix adjust. Identities = 235/488 (48%), Positives = 325/488 (66%), Gaps = 5/488 (1%) Query: 5 NIPHVPLYLNGEKVQSTAKEWRDVLNPATQEVVARVPFATKEEVDRAVANAKDTFKTWRN 64 ++PH+ ++NG+ +S + +DV NPAT A+V A+ +EV+ AVA A W Sbjct: 2 SLPHIDHFINGQISRSESGRSQDVFNPATGAATAKVALASVQEVNAAVAAASAAAPAWAE 61 Query: 65 TSLAQRMRIMLKFQQLLRENIAPLAELITREHGKTLPDAEGEVMRGLEVVEHACSITSLQ 124 T+ +R R++ KF++L+ +N LA+ IT EHGK DA+GEV RGLE+VE A I L Sbjct: 62 TAPLKRARVLFKFKELIEQNHDALAKAITLEHGKVFSDAKGEVTRGLEIVEFATGIPQLL 121 Query: 125 LGELAENVAGGVDVYTLYQPLGVGAGITAFNFPVMLPCFMFPIAVACGNTFVLKPSEQDP 184 + +N+ GG+D + L QPLGV AGIT FNFPVM+P +M PIA+A GN+FVLKPSE+DP Sbjct: 122 KTQFTDNIGGGIDNWNLRQPLGVTAGITPFNFPVMVPLWMAPIAIATGNSFVLKPSERDP 181 Query: 185 TSSLFLVELANQAGLPPGVLNVVHGGPDVANMICDHPDIKAVSFIGSTHVGTHIYRRASE 244 + SL L EL +AGLP GV NVV G + + +HP+++AVSF+GST + +IYR+A+E Sbjct: 182 SPSLMLAELFKRAGLPDGVFNVVQGDKVAVDALLEHPEVQAVSFVGSTPIAEYIYRKATE 241 Query: 245 -AGK---RAQCMMGAKNHCIVLPDAPKDQAINNLLGAAFGAAGQRCMANSVVVLVGQTRE 300 AG RAQ + GAKNH +V+PDA DQA++ L+GAA+G+AG+RCMA SV V VG + Sbjct: 242 RAGTFPLRAQALGGAKNHLVVMPDANLDQAVDALIGAAYGSAGERCMAISVAVAVGSVAD 301 Query: 301 WIPE-IVERSKTMKVGPGTDRKADVGPLVSKAAKERVERLIASGVEQGAHLLLDGRNCKV 359 + E ++ R K +KV G + A++GPLV+ A K +VE I SGV+ GA LL+DGR KV Sbjct: 302 KLVEALIPRVKALKVKNGMEDDAEMGPLVTAAHKAKVEGYIQSGVDAGAKLLVDGRGLKV 361 Query: 360 AGSENGNFVGPTVFTGVKPDMDIYTQEIFGPAMCIVELETLDEAIAFINANPNGNGTSIF 419 G E G F+G T+F V +M IY +EIFGP +C+V + A+ INA+ GNG S+F Sbjct: 362 PGHEEGFFIGGTLFDHVTTEMKIYQEEIFGPVLCVVRVPDFASAVQLINAHEYGNGVSLF 421 Query: 420 TSSGYAARKFQNEIDVGQVGINVPIPVPVAYFSFTGSRASKLGDLGPNGKQAVTFWTQTK 479 T+ G A +F I VG VGINVPIPVP+A+ SF G + S GD G++ + F+T+ K Sbjct: 422 TADGNTAHEFSRRIQVGMVGINVPIPVPMAWHSFGGWKRSLFGDTHAYGEEGIRFYTRYK 481 Query: 480 TVTARWYA 487 ++ RW A Sbjct: 482 SIMQRWSA 489 >gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 477 Score = 220 bits (561), Expect = 2e-58, Method: Compositional matrix adjust. Identities = 151/476 (31%), Positives = 227/476 (47%), Gaps = 9/476 (1%) Query: 9 VPLYLNGEKVQSTAKEWRDVLNPATQEVVARVPFATKEEVDRAVANAKDTFKTWRNTSLA 68 V L++NGE S + DV+NPAT+EV+ ++ A + ++DRA+ A F+TWRN S Sbjct: 5 VQLFINGEWTASASGRTIDVINPATEEVIGKIAHADRADLDRALEAASKGFETWRNMSAF 64 Query: 69 QRMRIMLKFQQLLRENIAPLAELITREHGKTLPDAEGEVMRGLEVVEHACSITSLQLGEL 128 +R +IM + LLRE +A L+T E GK L +A+ E + + ++ G L Sbjct: 65 ERSKIMRRAADLLRERAEEVARLMTMEQGKPLAEAKMETLGAADTIDWFAEEARRTYGRL 124 Query: 129 AENVAGGVDVYTLYQPLGVGAGITAFNFPVMLPCFMFPIAVACGNTFVLKPSEQDPTSSL 188 G + +P+G A T +NFP+ A+A G + ++K E+ P S Sbjct: 125 VPARVPGAYQMVIKEPVGPVAAFTPWNFPLNQVVRKLSAALAAGCSIIVKAPEETPASPA 184 Query: 189 FLVELANQAGLPPGVLNVVHGGP-DVANMICDHPDIKAVSFIGSTHVGTHIYRRASEAGK 247 L+ AG+P GV+N+V+G P +++ + HP IK +SF GST VG + A + K Sbjct: 185 ELIRAYADAGVPAGVINLVYGVPAEISEYLIPHPVIKKISFTGSTPVGKQLAALAGQHMK 244 Query: 248 RAQCMMGAKNHCIVLPDAPKDQAINNLLGAAFGAAGQRCMA-NSVVVLVGQTREWIPEIV 306 RA +G +V DA D A L A F AGQ C++ +V G ++ + V Sbjct: 245 RATMELGGHAPAMVFDDADVDVAAKMLATAKFRNAGQVCVSPTRFLVQKGVFNAFVDKFV 304 Query: 307 ERSKTMKVGPGTDRKADVGPLVSKAAKERVERLIASGVEQGAHLLLDGRNCKVAGSENGN 366 ++ +KVG G + +GP+ +K +E LI V QGA L G+ G Sbjct: 305 TYAEQLKVGNGLEAGITMGPVANKRRIPALEALIDDAVSQGAQLRTGGKRI----GNKGY 360 Query: 367 FVGPTVFTGVKPDMDIYTQEIFGPAMCIVELETLDEAIAFINANPNGNGTSIFTSSGYAA 426 F PTV T + +E FGP I ETLDE I N P G F S + Sbjct: 361 FFQPTVLTNLPLQARAMNEEPFGPLALINSFETLDEVITEANRLPYGLAAYAFAKSAHTK 420 Query: 427 RKFQNEIDVGQVGINVPIPVPVAYFSFTGSRASKLGDLGPNGKQAVTFWTQTKTVT 482 ++ G + IN + + + F G + S G G G +A+ + TK VT Sbjct: 421 HALAARVESGMLTIN-HLGLALPELPFGGIKDSGYGSEG--GTEAIEAYINTKLVT 473 >gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 199 bits (506), Expect = 5e-52, Method: Compositional matrix adjust. Identities = 135/458 (29%), Positives = 222/458 (48%), Gaps = 7/458 (1%) Query: 12 YLNGEKVQSTAKEWRDVLNPATQEVVARVPFATKEEVDRAVANAKDTFKTWRNTSLAQRM 71 ++NG+ + E V NPA E++ VP E RA+ A + W+ + +R Sbjct: 15 FVNGQWCDADQGERLAVHNPANGELLGHVPLMGAAETRRAIEAANAAWPAWKKKTAKERS 74 Query: 72 RIMLKFQQLLRENIAPLAELITREHGKTLPDAEGEVMRGLEVVEHACSITSLQLGELAEN 131 I+ ++ +L+ N LA ++T E GK L +A GE+ +E G+ + Sbjct: 75 AILRRWYELMLANTDDLALIMTAEQGKPLAEARGEIGYAASFIEWFAEEGKRTYGDTIPS 134 Query: 132 VAGGVDVYTLYQPLGVGAGITAFNFPVMLPCFMFPIAVACGNTFVLKPSEQDPTSSLFLV 191 + + + + +GV A IT +NFP + A+A G VLKP+E P S+L L Sbjct: 135 PSPSNRIVVIKEAIGVCAAITPWNFPAAMITRKAGPALAAGCPMVLKPAEATPFSALALA 194 Query: 192 ELANQAGLPPGVLNVVHGGPD-VANMICDHPDIKAVSFIGSTHVGTHIYRRASEAGKRAQ 250 LA +AG+P GV +VV G P + + +P ++ +SF GST VG + ++++ + K+ Sbjct: 195 VLAERAGIPAGVFSVVTGTPKGIGGEMTSNPIVRKISFTGSTGVGKLLMQQSASSIKKLS 254 Query: 251 CMMGAKNHCIVLPDAPKDQAINNLLGAAFGAAGQRCM-ANSVVVLVGQTREWIPEIVERS 309 +G IV DA D A+ + + + AGQ C+ AN + V G + ++VE Sbjct: 255 LELGGNAPFIVFDDADLDAAVEGAIASKYRNAGQTCVCANRIYVQDGVYDAFAAKLVEAV 314 Query: 310 KTMKVGPGTDRKADVGPLVSKAAKERVERLIASGVEQGAHLLLDGRNCKVAGSENGNFVG 369 K KVG GT+ GPL+++ A ++VE+ +A V +GA +LL G+ + S F Sbjct: 315 KKFKVGQGTEEGVTQGPLINEQAVQKVEQHVADAVAKGARVLLGGKRHALGHS----FFE 370 Query: 370 PTVFTGVKPDMDIYTQEIFGPAMCIVELETLDEAIAFINANPNGNGTSIFTSSGYAARKF 429 PTV V P M + +E FGP + +T +E +A N G + ++ + Sbjct: 371 PTVLADVTPAMQVAREETFGPMAPLFRFKTDEEVLALANDTEFGLASYFYSRDIGRIWRV 430 Query: 430 QNEIDVGQVGINVPIPVPVAYFSFTGSRASKLGDLGPN 467 ++ G VGIN + + F G + S LG G + Sbjct: 431 AEGLESGMVGINTGL-ISNEVAPFGGVKQSGLGREGSH 467 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.134 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,567,645 Number of extensions: 66177 Number of successful extensions: 264 Number of sequences better than 1.0e-08: 18 Number of HSP's gapped: 218 Number of HSP's successfully gapped: 18 Length of query: 502 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 413 Effective length of database: 1,198,455 Effective search space: 494961915 Effective search space used: 494961915 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)