BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3021.p (481 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312357|ref|YP_003776449.1| cobalt/zinc/cadmium resistance ... 776 0.0 gi|300309362|ref|YP_003773454.1| transmembrane sensory transduct... 290 2e-79 gi|300311800|ref|YP_003775892.1| two component heavy metal senso... 189 3e-49 gi|300313246|ref|YP_003777338.1| two component transmembrane sen... 102 8e-23 gi|300312679|ref|YP_003776771.1| periplasmic sensor signal trans... 99 7e-22 >gi|300312357|ref|YP_003776449.1| cobalt/zinc/cadmium resistance two component transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 475 Score = 776 bits (2004), Expect = 0.0, Method: Compositional matrix adjust. Identities = 406/474 (85%), Positives = 428/474 (90%) Query: 1 MSQATVHARGWTLTTRVTALFALMTCLVVTGLGLYLDRAAGDALAQRADQALIGRVEHFR 60 MS T GWTLTTRVTALFALMT LVVTGLG YLDR+AG AL+QRADQALIGRVEHFR Sbjct: 1 MSPPTPAVGGWTLTTRVTALFALMTSLVVTGLGFYLDRSAGQALSQRADQALIGRVEHFR 60 Query: 61 NLMHDLYNVEQMEQRPALFESMMGNEQDVRIFRRQGEAPFIQSNPDHLVPPAMQPAPVDQ 120 NLMHDLYNVEQMEQRPALFESMMGNEQDVRIFRRQGEAPFIQSNPDHLVPPAM P PV Sbjct: 61 NLMHDLYNVEQMEQRPALFESMMGNEQDVRIFRRQGEAPFIQSNPDHLVPPAMTPLPVGS 120 Query: 121 PVSAARLLTSERADGVRVRWVSALADVGKGRDTVEIIAAYVMVQEDRMMRSYRWRIAGAA 180 PVS+A L TS+RADGVRVRWVSALADVGKG DTVEI+AAYVMVQE RMMRSYRWRIAGAA Sbjct: 121 PVSSASLRTSQRADGVRVRWVSALADVGKGGDTVEIVAAYVMVQEARMMRSYRWRIAGAA 180 Query: 181 AAAILLTSLLGFVLLRRGLQPLEAMSQRAAQITPDRLSTRLEQEGMPAELRRVALSFNAM 240 AAA++LT+LLGF+LLRRGLQPLEAMSQRAAQITPD LS RL+QEGMPAELRRVALSFNAM Sbjct: 181 AAAVVLTALLGFLLLRRGLQPLEAMSQRAAQITPDHLSARLDQEGMPAELRRVALSFNAM 240 Query: 241 LDRLEAGYDHLAQFSGDLAHEIRTPINVLMGQSQVALGQRRSPEDYEQLLESNVEELQRL 300 LDRLEAGY HLAQFSGDLAHEIRTPINVLMGQSQVALGQRRSPE+YEQ+LESNVEELQRL Sbjct: 241 LDRLEAGYAHLAQFSGDLAHEIRTPINVLMGQSQVALGQRRSPEEYEQVLESNVEELQRL 300 Query: 301 ARIVENILFLAQADHSTLAVECSALDLQEELAKITDYFEGIADERGMHFALQAQGICQAN 360 +RIVENILFLAQADH+ LAVE +ALDL EEL +ITDYFEGIADERGM F +QA G CQAN Sbjct: 301 SRIVENILFLAQADHAALAVERNALDLAEELGRITDYFEGIADERGMRFDVQAAGQCQAN 360 Query: 361 LDMWRRAVSNLIINAVRYGEPDSIIRIQAEGDASGSRILVDNRSGNVTPQVMARMFDRFY 420 L MWRR VSNL+INAVRYG +S IRI AE D GSRI+V+NRS +VT Q MARMFDRFY Sbjct: 361 LIMWRRVVSNLVINAVRYGRAESTIRILAESDGEGSRIMVENRSEDVTAQAMARMFDRFY 420 Query: 421 RGDRSRSALTESNGLGLAIVKAIMALHRGTATVECPQPGWIRFSLRFPSVAAAP 474 RGDRSRSA TESNGLGLAIVKAIMALH GTA VECP PGWIRFSLRFPS AA P Sbjct: 421 RGDRSRSAFTESNGLGLAIVKAIMALHGGTAAVECPAPGWIRFSLRFPSAAAVP 474 >gi|300309362|ref|YP_003773454.1| transmembrane sensory transduction histidine kinase for cobalt/zinc/ cadmium resistance transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 467 Score = 290 bits (741), Expect = 2e-79, Method: Compositional matrix adjust. Identities = 177/465 (38%), Positives = 275/465 (59%), Gaps = 10/465 (2%) Query: 12 TLTTRVTALFALMTCLVVTGLGLYLDRAAGDALAQRADQALIGRVEHFRNLMHDLYNVEQ 71 +L+ R+ ALF L+ +++ LG YL + L +R D+ L G+ +L+ ++E Sbjct: 8 SLSLRLVALFGLLAMVILAALGTYLSHSLHVQLERRDDEELAGKCRSINHLVEHAASLED 67 Query: 72 -MEQRPALFESMMGNEQD-VRIFRRQGEAPFIQSNPDHLVPPAMQPAPVDQPVSAARLLT 129 + +R + +++ G++Q VRI R G + ++S+ PP + + VD + A + Sbjct: 68 ILARRHLVLDTLTGHDQLLVRIARADG-STLLESHDPTRTPPGIDLSQVD--IRAWDSIQ 124 Query: 130 SERADGVRVRWVSALADVGKGRDTVEIIAAYVMVQEDRMM--RSYRWRIAGAAAAAILLT 187 S + +R R+V++ G V ++ V DRM+ YR + AA LL Sbjct: 125 SWKLGDLRARYVASKPVTRSGEQAVVLV---VRTASDRMLLLAEYRSDVWWAAGVGALLA 181 Query: 188 SLLGFVLLRRGLQPLEAMSQRAAQITPDRLSTRLEQEGMPAELRRVALSFNAMLDRLEAG 247 L+ + L+R GL+PL M+ + ++ +RL TRL+ + P EL + SFN MLDRL Sbjct: 182 ILMSYFLVRSGLRPLRTMAAQTRAVSANRLDTRLDLQAAPRELHEIVESFNEMLDRLHRS 241 Query: 248 YDHLAQFSGDLAHEIRTPINVLMGQSQVALGQRRSPEDYEQLLESNVEELQRLARIVENI 307 + L+QFS DLAH++RTP+N LM Q+QVALG+ R ++Y+ LL SN+EE +RL+R++E++ Sbjct: 242 FQQLSQFSADLAHDVRTPLNNLMVQTQVALGKPRGIDEYQALLSSNIEEYERLSRMMESM 301 Query: 308 LFLAQADHSTLAVECSALDLQEELAKITDYFEGIADERGMHFALQAQGICQANLDMWRRA 367 LFLA+A+++ + ++ LD+ +EL +I DYFEGIADE + +Q G QA+ + RRA Sbjct: 302 LFLARAENAHVVLQWQELDVAQELTRIADYFEGIADEAQVRLVVQGAGRLQADATLLRRA 361 Query: 368 VSNLIINAVRYGEPDSIIRIQAEGDASGSRILVDNRSGNVTPQVMARMFDRFYRGDRSRS 427 V NL+ NA+RY D +IR+Q + G I V N + + + R+FDRFYR D SRS Sbjct: 362 VGNLVANAIRYTPADGVIRLQVDAAGDGMEISVSNPGAGIPAEQLERLFDRFYRADPSRS 421 Query: 428 ALTESNGLGLAIVKAIMALHRGTATVECPQPGWIRFSLRFPSVAA 472 + S+GLGLAIV++IM LH G A+V FSLRFP +A Sbjct: 422 SAGGSSGLGLAIVRSIMMLHGGQASVTSSPGQLTVFSLRFPGRSA 466 >gi|300311800|ref|YP_003775892.1| two component heavy metal sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 467 Score = 189 bits (481), Expect = 3e-49, Method: Compositional matrix adjust. Identities = 104/273 (38%), Positives = 162/273 (59%), Gaps = 4/273 (1%) Query: 183 AILLTSLLGFVLLRRGLQPLEAMSQRAAQITPDRLSTRLEQEGMPAELRRVALSFNAMLD 242 I+L + + + R GL PL MS RA +P +LS R+ E MP EL +AL+FN LD Sbjct: 177 GIVLAVISSYWIARAGLAPLRQMSDRAQSFSPRKLSARMPVEAMPQELSVLALAFNGALD 236 Query: 243 RLEAGYDHLAQFSGDLAHEIRTPINVLMGQSQVALGQRRSPEDYEQLLESNVEELQRLAR 302 R+E Y L F+ D+AHE+RTP+ L+G++QVAL + RS +++ +L+SN+EEL R+ Sbjct: 237 RIEDAYTQLEAFNADVAHELRTPLANLIGETQVALARERSAGEFKDVLQSNLEELDRIRS 296 Query: 303 IVENILFLAQADHSTLAVECSALDLQEELAKITDYFEGIADERGMHFALQAQGICQANLD 362 IV ++LFLA++D A + +E+ K D+FE + D+ GM + A ++ Sbjct: 297 IVNDMLFLARSDQGEAATARVPARVADEVQKTVDFFEFVLDDSGMRIEVAGDTEATAMME 356 Query: 363 --MWRRAVSNLIINAVRYGEPDSIIRIQAEGDASGSRILVDNRSGNVTPQVMARMFDRFY 420 +++RA++NLI NA+++ +++RI R+ V N + + R+FDRFY Sbjct: 357 TALFKRALTNLIQNAIQHSPEGALLRIDIACQEETVRVAVSNPGKPIDEHHLPRLFDRFY 416 Query: 421 RGDRSRSALTES--NGLGLAIVKAIMALHRGTA 451 R D +R + S +GLGLAIVKA+ +H G A Sbjct: 417 RVDSARQDINGSHGHGLGLAIVKAVARMHGGRA 449 >gi|300313246|ref|YP_003777338.1| two component transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 361 Score = 102 bits (253), Expect = 8e-23, Method: Compositional matrix adjust. Identities = 83/296 (28%), Positives = 142/296 (47%), Gaps = 15/296 (5%) Query: 184 ILLTSLLGFV--------LLRRGLQPLEAMSQRAAQITPDRLSTRLEQEGMPA-ELRRVA 234 IL+T+L+G L RR L PL +++ ++ LS R E P E ++ Sbjct: 69 ILVTTLIGLSIALWVAVRLSRRILTPLNSVATSLRRVAQGDLSARAAAERQPLDEAWQLI 128 Query: 235 LSFNAMLDRLEAGYDHLAQFSGDLAHEIRTPINVLMGQSQVALGQRRSPEDYEQLLESNV 294 FNAM +RLE ++ +AHE+RTP+ +L G+ Q PE L S + Sbjct: 129 NDFNAMAERLERVQQERIFWNAAIAHELRTPVTILRGRLQGLSDGVFQPE--PALFSSLL 186 Query: 295 EELQRLARIVENILFLAQADHSTLAVECSALDLQEELAKITDYFEGIADERGMHFALQAQ 354 +++ L R++E++ + A+ L V+ DLQ ++ + E E+G L+ Sbjct: 187 AQVEGLGRLIEDLRIVGLAESGHLRVQLQETDLQADIMTVLKACEPALQEKGFRLLLET- 245 Query: 355 GICQANLD--MWRRAVSNLIINAVRYGEPDSIIRIQAEGDASGSRILVDNRSGNVTPQVM 412 G+ ++ D R+A+ L+ N +++ P + RI A + V++ + Sbjct: 246 GVQRSRCDPVRIRQALLALLENVLQHASPGPL-RISTRSLAGMHSLCVEDSGPGIAASDA 304 Query: 413 ARMFDRFYRGDRSRSALTESNGLGLAIVKAIMALHRGTATVECPQPGWIRFSLRFP 468 +FD F+RG R+ A + +GLGLA+V+ I + H GTA + G F +R+P Sbjct: 305 QMIFDAFHRGARAGDAQQKGSGLGLAVVRTIASAHHGTADCRASELGGACFEVRWP 360 >gi|300312679|ref|YP_003776771.1| periplasmic sensor signal transduction histidine kinase [Herbaspirillum seropedicae SmR1] Length = 459 Score = 99.0 bits (245), Expect = 7e-22, Method: Compositional matrix adjust. Identities = 74/287 (25%), Positives = 139/287 (48%), Gaps = 11/287 (3%) Query: 192 FVLLRRGLQPLEAMSQRAAQITPDRLSTRLEQEGMPAELRRVALSFNAMLDRLEAGYDHL 251 V +RRGL+PL+++ + Q +L ++ G P EL+ + ++ N ++ R+ G Sbjct: 179 LVAVRRGLRPLKSLQRNLEQRDHGKLGA-IDGRGAPVELQPLIVAMNELMARINKGEQAQ 237 Query: 252 AQFSGDLAHEIRTPINVLMGQSQVALGQRRSPEDYEQLLESNVEELQRLARIVENILFLA 311 F D+AH++RTP+ L Q ++ + + D + L ++R+ R +L LA Sbjct: 238 QNFMADVAHQLRTPLTGLKLQLELLHDKHQDQPDTARSLAMMNSSVERMIRQSRQLLALA 297 Query: 312 QADHSTL-AVECSALDLQEELAKITDYFEGIADERGMHFALQAQ-GICQANLDMWRRAVS 369 +++ + + L L + + + +F AD++ + + + + + + Sbjct: 298 RSEPGLFESRKLEELSLDKLVEESVQHFIEEADKKRIDLGFDLRPARLRGDRFLLSDLID 357 Query: 370 NLIINAVRYGEPDSIIRIQAEGDASGSRILVDNRSGNVTPQVMARMFDRFYRGDRSRSAL 429 NLI NAVRY P + ++ + + + V++ + P+ +FDRFYR + Sbjct: 358 NLIDNAVRYSPPHGTVTVRCLEEQDATVLSVEDSGPGIAPEHRELIFDRFYRANDK---- 413 Query: 430 TESNGLGLAIVKAIMALHRGTATVECPQPGW-IRFSLRFPSVAAAPP 475 +GLGLAIV+ I H G +V+C + G F++RFP APP Sbjct: 414 VAGSGLGLAIVREIAHDHGGVISVDCKEQGQGTIFTVRFP---LAPP 457 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.134 0.388 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,447,802 Number of extensions: 59358 Number of successful extensions: 340 Number of sequences better than 1.0e-08: 26 Number of HSP's gapped: 308 Number of HSP's successfully gapped: 26 Length of query: 481 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 392 Effective length of database: 1,198,455 Effective search space: 469794360 Effective search space used: 469794360 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 132 (55.5 bits)