BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.3012.p (392 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312349|ref|YP_003776441.1| hemolysin-type secretion transm... 716 0.0 gi|300311130|ref|YP_003775222.1| Type I antifreeze protein:HlyD ... 187 9e-49 gi|300312188|ref|YP_003776280.1| hemolysin secretion-related tra... 144 1e-35 >gi|300312349|ref|YP_003776441.1| hemolysin-type secretion transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 386 Score = 716 bits (1847), Expect = 0.0, Method: Compositional matrix adjust. Identities = 351/386 (90%), Positives = 369/386 (95%) Query: 7 MKKNNSHNPLVSARWMLRLSMLSVVLLLVWAAVSPIDQVTRAQGQVIAVSRTQSVQAPDG 66 M+ NN NPL+SA+W+LRLSM SV++LL+WAAVS IDQVTRAQGQVIAVSRTQSVQAPDG Sbjct: 1 MEMNNEKNPLISAQWVLRLSMASVLVLLLWAAVSQIDQVTRAQGQVIAVSRTQSVQAPDG 60 Query: 67 GPVKQILVKEGEAVKQGQLLMVLEQDRVQTSLNDSRAKVAALRISLARLRAEVYGQPLVF 126 GPVKQILVKEGEAVKQGQLLMVLEQDRVQTSLNDSRAKVAALRISLARLRAEVYG PL F Sbjct: 61 GPVKQILVKEGEAVKQGQLLMVLEQDRVQTSLNDSRAKVAALRISLARLRAEVYGHPLNF 120 Query: 127 DPDLLDYKEYISNQKDLYVKRKTLIDQDLQALNDMLGLASQELEMNRNLEKTGDVGRADV 186 +PDLLDYKEYI+NQKDLYVKRK LIDQDL +L DMLGLA+QELEMN++LE+TGDVGRADV Sbjct: 121 EPDLLDYKEYIANQKDLYVKRKILIDQDLASLQDMLGLANQELEMNKSLERTGDVGRADV 180 Query: 187 LRLQRNVADIRAQINNKRNKYFQEALVDMTKVQEDLNTQREQLEDRSQVLEHTELKAPAD 246 LRLQRNVADIRAQINNKRNKYFQEALVDMTKVQEDLNTQREQLEDR+QVLEHTELKAPAD Sbjct: 181 LRLQRNVADIRAQINNKRNKYFQEALVDMTKVQEDLNTQREQLEDRTQVLEHTELKAPAD 240 Query: 247 GVVKNIRVTTIGGVVRPGDVVMEILPVDNLIVEVKVSTADSAYVKVGQQANVKLDAYDYS 306 GVVKNI+VTTIGGVVR GDVVMEILPVDNLIVE KVSTADSAYVKVGQ ANVKLDAYDYS Sbjct: 241 GVVKNIKVTTIGGVVRSGDVVMEILPVDNLIVEAKVSTADSAYVKVGQAANVKLDAYDYS 300 Query: 307 IYGTLNGHVVYVSPDTLLDETRQGAVPYYRVHIALEEAVFKGEKSSAIHVKPGMTATVEI 366 IYGTLNGHVVYVSPDTLLDETRQGA+PYYRVHIALEEAVFKG K+ AI VKPGMTATVEI Sbjct: 301 IYGTLNGHVVYVSPDTLLDETRQGAMPYYRVHIALEEAVFKGRKADAIQVKPGMTATVEI 360 Query: 367 KANTRTVLSFLVKPIAKTLQESMGER 392 KANTRTVLSFL KPIAKTLQESMGER Sbjct: 361 KANTRTVLSFLTKPIAKTLQESMGER 386 >gi|300311130|ref|YP_003775222.1| Type I antifreeze protein:HlyD family secretion protein [Herbaspirillum seropedicae SmR1] Length = 457 Score = 187 bits (476), Expect = 9e-49, Method: Compositional matrix adjust. Identities = 123/400 (30%), Positives = 206/400 (51%), Gaps = 46/400 (11%) Query: 36 WAAVSPIDQVTRAQGQVIAVSRTQSVQAPDGGPVKQILVKEGEAVKQGQLLMVLEQDRVQ 95 W+ +PID V R G+++A Q +Q +GG V ILV+EG AVK+GQ L+ + R Sbjct: 58 WSVFAPIDMVVRGSGRIVASEHNQVIQHLEGGIVSAILVREGAAVKKGQTLISISDVRAN 117 Query: 96 TSLNDSRAKVAALRISLARLRAEVYGQPLVFDPDLLDYKE-YISNQKDLYVKRKTLIDQD 154 L + R K+ LR +ARL AE G + PD L+ + + +++ + R + + Q+ Sbjct: 118 ADLGEGRVKILGLRAKIARLSAEASGASSLQMPDDLERGDPAVQSEQAAFAARSSKLMQE 177 Query: 155 LQALNDM-------LGLA-------SQELEMN------------RN----LEKTGDVGR- 183 + L + LG A + ELE+ RN LE R Sbjct: 178 MSVLREQSAQRQAELGEAISRQKSQASELELAQRQYKLIHDMLVRNAASQLEDIQAASRV 237 Query: 184 -----------ADVLRLQRNVADIRAQINNKRNKYFQEALVDMTKVQEDLNTQREQLEDR 232 A + RL +A+ +A+ +++ +A ++T + +L +E+++ R Sbjct: 238 QDAQSKLAATNAMIPRLNAAIAEAQAKQGEAASRFRSDARTELTAAETELARLQEEIKSR 297 Query: 233 SQVLEHTELKAPADGVVKNIRVTTIGGVVRPGDVVMEILPVDN-LIVEVKVSTADSAYVK 291 + + +E+KAP DGVV + + TIGGVVRPGD ++E+ P D+ L++E ++S D A + Sbjct: 298 NDRVSRSEVKAPMDGVVNRVMINTIGGVVRPGDPIIEMTPSDDKLVIEARISPTDRAELV 357 Query: 292 VGQQANVKLDAYDYSIYGTLNGHVVYVSPDTLLDETRQGAVPYYRVHIALEEAVFKGEKS 351 G +A VK+ AYDY +YG ++G V +S DT+ DE +YR+ I ++ E Sbjct: 358 TGARARVKVSAYDYGVYGAMDGVVTEISADTVPDEGSPRGERFYRLKIEVKRG--HDELQ 415 Query: 352 SAIHVKPGMTATVEIKANTRTVLSFLVKPIAKTLQESMGE 391 + + PGMTA+ +I RTV +L+ P+++ Q ++ E Sbjct: 416 QKLALMPGMTASADIIVGRRTVWQYLMSPLSRFGQGALRE 455 >gi|300312188|ref|YP_003776280.1| hemolysin secretion-related transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 447 Score = 144 bits (364), Expect = 1e-35, Method: Compositional matrix adjust. Identities = 119/401 (29%), Positives = 199/401 (49%), Gaps = 37/401 (9%) Query: 18 SARWMLRLSMLSVVLLLVWAAVSPIDQVTRAQGQVIAVSRTQSVQAPDGGPVKQILVKEG 77 S RW RL + V+ L+W+ + ID V A G+VI +RT+++ + D V+ ILV EG Sbjct: 58 SLRWTARLLVGMVIFALLWSLLGRIDIVVSANGKVIPSARTKTIASVDVSAVRAILVSEG 117 Query: 78 EAVKQGQLLMVLEQDRVQTSLNDSRAKVA--------------ALRISLARLRAEVYGQ- 122 + VK G++L+ L+ +S + R K A A+ ++ LR Q Sbjct: 118 QLVKAGEVLIELD----SSSSDAERDKAADAVAQARLQAARAQAMMEAIQNLRTPELAQV 173 Query: 123 PLVFDPDLLDYKEYISNQKDLYVKRKTLIDQDLQALNDMLGLASQELEMNRNLEKTGDVG 182 P V + + Q + R +D ++ L LA+Q + L V Sbjct: 174 PGVAQAQWEAVRSQLVAQYQDFRARLGRLDDEISRYGAALKLATQRASDYQTLVADHTVS 233 Query: 183 RADVLRLQRNVADIRAQINNKRNKYFQEALVDMT-------KVQEDLNTQREQLEDRSQV 235 R L ++ D++ Q+++ RN+ + LV T +++ D + Q +D+ + Sbjct: 234 RHAWLEKEQARVDLQGQLDDARNQ--RATLVAQTRKDAHDSRIEADKTIEASQ-QDQRRA 290 Query: 236 LEHT---ELKAPADGVVKNIRVTTIGGVVRPGDVVMEILPVDNLI-VEVKVSTADSAYVK 291 EH+ +L AP G V+ + V TIGGVV +M+I+P D+ I VE + D +V+ Sbjct: 291 DEHSKLLKLVAPVTGTVQQLTVHTIGGVVPAAQPLMQIVPQDSQIEVEAMLENKDVGFVE 350 Query: 292 VGQQANVKLDAYDYSIYGTLNGHVVYVSPDTLLDETRQGAVPYYRVHIALEEAVFKGEKS 351 +GQ A VK+DA+DY+ YGTL VV++S D + DE ++G + R+ + + G K Sbjct: 351 IGQAAEVKIDAFDYTKYGTLPARVVHISRDAIQDE-KKGLLYSARIVLDQSSLIVDGRK- 408 Query: 352 SAIHVKPGMTATVEIKANTRTVLSFLVKPIAKTLQESMGER 392 + + GM A VEIK R V+ + + P+ + +E++ ER Sbjct: 409 --VQLSAGMAANVEIKTGNRRVIEYALSPLIRHQKEALHER 447 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.132 0.356 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 965,889 Number of extensions: 34595 Number of successful extensions: 173 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 170 Number of HSP's successfully gapped: 6 Length of query: 392 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 305 Effective length of database: 1,207,925 Effective search space: 368417125 Effective search space used: 368417125 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 131 (55.1 bits)