BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2999.p (536 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312336|ref|YP_003776428.1| methyl-accepting chemotaxis tra... 809 0.0 gi|300310594|ref|YP_003774686.1| methyl-accepting chemotaxis pro... 544 e-156 gi|300313938|ref|YP_003778030.1| methyl-accepting chemotaxis tra... 510 e-145 gi|300310668|ref|YP_003774760.1| methyl-accepting chemotaxis tra... 486 e-138 gi|300312038|ref|YP_003776130.1| methyl-accepting chemotaxis tra... 446 e-126 >gi|300312336|ref|YP_003776428.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 536 Score = 809 bits (2089), Expect = 0.0, Method: Compositional matrix adjust. Identities = 434/532 (81%), Positives = 476/532 (89%) Query: 1 MNILGNMNIGKRLTLGFAVILAFSAVVAAISLWRLEQVASSTREMMNAPLKTERLVADWY 60 MNI+GNMNIG+RLTLGFA+IL FS ++A I LWRL+ VA +TREMM PLKTERL+ADWY Sbjct: 1 MNIIGNMNIGRRLTLGFALILTFSIIIAGIGLWRLDHVAQATREMMAEPLKTERLMADWY 60 Query: 61 TNVNAGIARTLAIAKSADASLGPYFSKDVTASSKMSSEYQKQIQTLLSTPEEKALFDKIG 120 TN+ AGI RT+AIAKS+D +LGPYF+ +V ASSK S EYQKQ++ LLSTPEEKALF KIG Sbjct: 61 TNLVAGIRRTIAIAKSSDPALGPYFAAEVAASSKSSGEYQKQVEALLSTPEEKALFQKIG 120 Query: 121 EQRKIYLSSRDEIIKLKAAGNVEEAMRVLDKVFVPNAAVFQQLMRQLVDIQRKEMDESAM 180 E RK+YLSSRDEI K KAAGN EEAMR+LD+VFVPNA +Q MR+LVD QRKEMD++A Sbjct: 121 ELRKLYLSSRDEINKAKAAGNTEEAMRILDQVFVPNATAYQDTMRKLVDFQRKEMDDTAR 180 Query: 181 QIDAISAQSHIIILVLEGLILLLGIVLARILTLGITKPLQNAVVVSRKVAAGDLSASVQV 240 +IDAI+A+S +ILVLEGLILLLGIV AR LTLGITKPL AV VSR VA GDLS+ + V Sbjct: 181 EIDAIAAKSRTLILVLEGLILLLGIVFARYLTLGITKPLHRAVDVSRCVAEGDLSSEIAV 240 Query: 241 HSQDETGQLLQALKDMNDSLRGIVSNVRSGTDTISTASSEIATGNLDLSSRTEEQAGSLA 300 S+DETGQLLQ+LKDMN SLRGIVSNVRSGTDTISTASSEIA GNLDLSSRTE+QA SLA Sbjct: 241 TSRDETGQLLQSLKDMNQSLRGIVSNVRSGTDTISTASSEIAAGNLDLSSRTEQQASSLA 300 Query: 301 ETASAMEQLISTVRQNADNARQASQLAQSASSVAEQGGGVVSRVVDTMGAINASSRKIVD 360 ETASAME+LISTVRQNADNARQASQLA SAS+VAEQGGGVVS+VVDTMGAINASSRKIVD Sbjct: 301 ETASAMEELISTVRQNADNARQASQLANSASAVAEQGGGVVSQVVDTMGAINASSRKIVD 360 Query: 361 IIGVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSASAAKEIKELIHD 420 II VIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVR+LAQRSASAAKEIKELI+D Sbjct: 361 IISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRTLAQRSASAAKEIKELIND 420 Query: 421 SVAKVGDGSRLVEQAGSTMQEVVSSVRHVTDIVAEISAASAEQTNGIEQINLAITQMDQV 480 SV KV DGSRLVEQAG+TMQEVVSSVR VTDIVAEISAAS EQT+GIEQINLAITQMDQV Sbjct: 421 SVGKVDDGSRLVEQAGNTMQEVVSSVRRVTDIVAEISAASTEQTHGIEQINLAITQMDQV 480 Query: 481 TQQNAALVEQAAAAAASMQDQAGRLAQMVSIFRLHEGEALAQREIDVTPVMP 532 TQQNAALVE+AAAAAASMQ QAGRL QMVS+FRL E E L +REIDVTP P Sbjct: 481 TQQNAALVEEAAAAAASMQTQAGRLMQMVSVFRLSEREPLVRREIDVTPSSP 532 >gi|300310594|ref|YP_003774686.1| methyl-accepting chemotaxis protein I [Herbaspirillum seropedicae SmR1] Length = 541 Score = 544 bits (1401), Expect = e-156, Method: Compositional matrix adjust. Identities = 328/540 (60%), Positives = 407/540 (75%), Gaps = 6/540 (1%) Query: 1 MNILGNMNIGKRLTLGFAVILAFSAVVAAISLWRLEQVASSTREMMNAPLKTERLVADWY 60 M NM IG+RL LGFAVILA ++VV A+S+WRL+ +A++T EMM+ PL ERLV+DWY Sbjct: 1 MRYFANMKIGRRLALGFAVILAAASVVVALSIWRLQGIAAATDEMMHEPLTKERLVSDWY 60 Query: 61 TNVNAGIARTLAIAKSADASLGPYFSKDVTASSKMSSEYQKQIQTLLSTPEEKALFDKIG 120 ++ + RT AIAKS+D SL +F+ D A+S+MS+E QK I+ L+ + +EKA+F K+G Sbjct: 61 RTIHTSVRRTTAIAKSSDPSLAAFFADDAAAASRMSTEQQKAIEALIESDQEKAVFTKLG 120 Query: 121 EQRKIYLSSRDEIIKLKAAGNVEEAMRVLDKVFVPNAAVFQQLMRQLVDIQRKEMDESAM 180 E RK Y+ RD I K KA G EEA ++L F + L++QL+ +QR +D+ A Sbjct: 121 EVRKDYIKYRDAISKAKAEGRTEEAAQILAGPFDVAGKGYLDLLQQLLTLQRANIDQVAA 180 Query: 181 QIDAISAQSHIIILVLEGLILLLGIVLARILTLGITKPLQNAVVVSRKVAAGDLSASVQ- 239 I + +S ++L L L ++LG + AR L LGIT+PL+ AV V+ VAAGDL+ + Sbjct: 181 HIQTVYRESRNLMLALAFLQVVLGWLFARTLALGITRPLEQAVEVAEAVAAGDLTHRIDE 240 Query: 240 --VHSQDETGQLLQALKDMNDSLRGIVSNVRSGTDTISTASSEIATGNLDLSSRTEEQAG 297 DETG+L+ AL M +L IV VR GTD I+TAS EIATGNLDLS+RTE QAG Sbjct: 241 RLAARGDETGKLMHALHAMTGNLARIVGQVRGGTDAIATASKEIATGNLDLSARTERQAG 300 Query: 298 SLAETASAMEQLISTVRQNADNARQASQLAQSASSVAEQGGGVVSRVVDTMGAINASSRK 357 +L ETASAME+L STV QNADNARQA+QLA SASSVA+QGG VVS+V+ TMG+IN SSRK Sbjct: 301 ALEETASAMEELTSTVSQNADNARQANQLAASASSVAQQGGEVVSQVISTMGSINESSRK 360 Query: 358 IVDIIGVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSASAAKEIKEL 417 IVDII VIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSASAAKEIK L Sbjct: 361 IVDIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSASAAKEIKAL 420 Query: 418 IHDSVAKVGDGSRLVEQAGSTMQEVVSSVRHVTDIVAEISAASAEQTNGIEQINLAITQM 477 I DSVAKVG GS LV +AGSTM EVV+SVR V DIV+EISAAS+EQ++GI Q+NLAIT M Sbjct: 421 IDDSVAKVGSGSELVTRAGSTMDEVVASVRQVADIVSEISAASSEQSDGISQVNLAITDM 480 Query: 478 DQVTQQNAALVEQAAAAAASMQDQAGRLAQMVSIFRL--HEGEALAQRE-IDVTPVMPGI 534 DQ TQQNAALVE+AAAAA +MQ+QA RL+++VS F+L + A A R +D+TP +P + Sbjct: 481 DQTTQQNAALVEEAAAAAEAMQEQAARLSEVVSQFKLLGQDQIAYAPRSAVDITPPLPAL 540 >gi|300313938|ref|YP_003778030.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 533 Score = 510 bits (1314), Expect = e-145, Method: Compositional matrix adjust. Identities = 289/513 (56%), Positives = 362/513 (70%) Query: 4 LGNMNIGKRLTLGFAVILAFSAVVAAISLWRLEQVASSTREMMNAPLKTERLVADWYTNV 63 + N+ IG RL +GF +L AV+ A+ +WRL + +T +M LK ER W+ + Sbjct: 1 MKNLKIGVRLAIGFIALLMLMAVMVALGVWRLGSIGDATDDMTRIALKKERDAVQWHAAI 60 Query: 64 NAGIARTLAIAKSADASLGPYFSKDVTASSKMSSEYQKQIQTLLSTPEEKALFDKIGEQR 123 RT A+ KS D + YF K + A S S+ QK+++ ++ EEK LFD++G+ R Sbjct: 61 KENGVRTFAVMKSDDDAFQAYFQKQIDAQSARISQLQKEVEAAITDAEEKRLFDEVGKLR 120 Query: 124 KIYLSSRDEIIKLKAAGNVEEAMRVLDKVFVPNAAVFQQLMRQLVDIQRKEMDESAMQID 183 Y ++R I +K AG+ A + D V + + + D Q++ +D++A I+ Sbjct: 121 AAYRATRQSIFDVKTAGDDARAREMTDTTLVKMMDDYADGVLRYADFQKQVIDQTAAAIN 180 Query: 184 AISAQSHIIILVLEGLILLLGIVLARILTLGITKPLQNAVVVSRKVAAGDLSASVQVHSQ 243 ++L L + ++LG LA +LT IT+PL AV ++ VAAGDL++ + S+ Sbjct: 181 RDYRSGRSMLLALGAIEIVLGAALAWLLTRSITRPLNQAVTIAETVAAGDLTSRIDSSSR 240 Query: 244 DETGQLLQALKDMNDSLRGIVSNVRSGTDTISTASSEIATGNLDLSSRTEEQAGSLAETA 303 DETG+LL ALK MN +L IV VR GT+TI+TAS EIATGNLDLS+RTE QAGSL ETA Sbjct: 241 DETGKLLAALKTMNGNLLDIVGRVRMGTETINTASREIATGNLDLSARTEAQAGSLEETA 300 Query: 304 SAMEQLISTVRQNADNARQASQLAQSASSVAEQGGGVVSRVVDTMGAINASSRKIVDIIG 363 SAMEQL STV+QNADNARQA++LA SAS VA QGG VV +VV TMGAI+ASS+KI DIIG Sbjct: 301 SAMEQLTSTVKQNADNARQANELAASASQVATQGGAVVDQVVATMGAIHASSQKIADIIG 360 Query: 364 VIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSASAAKEIKELIHDSVA 423 VIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSASAAKEIK+LI DSV Sbjct: 361 VIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSASAAKEIKQLIDDSVE 420 Query: 424 KVGDGSRLVEQAGSTMQEVVSSVRHVTDIVAEISAASAEQTNGIEQINLAITQMDQVTQQ 483 KV DGSRLV +AG TMQEVV SV VT +V EI+ AS EQ++GIEQ+NLAIT +D+ TQQ Sbjct: 421 KVDDGSRLVARAGDTMQEVVQSVARVTAVVREITLASQEQSDGIEQVNLAITHIDETTQQ 480 Query: 484 NAALVEQAAAAAASMQDQAGRLAQMVSIFRLHE 516 NAALVEQAAAAA SMQDQA L VS+FRL + Sbjct: 481 NAALVEQAAAAARSMQDQAASLTDAVSVFRLED 513 >gi|300310668|ref|YP_003774760.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 515 Score = 486 bits (1252), Expect = e-138, Method: Compositional matrix adjust. Identities = 281/513 (54%), Positives = 370/513 (72%) Query: 3 ILGNMNIGKRLTLGFAVILAFSAVVAAISLWRLEQVASSTREMMNAPLKTERLVADWYTN 62 ++ ++ IG RL GFAV+L FS VVAA+ +WRL+ VA T++MM+ PLKTERLV+ W T Sbjct: 1 MIKDIRIGSRLGAGFAVVLVFSMVVAAVGIWRLKTVAVQTQQMMDVPLKTERLVSQWNTL 60 Query: 63 VNAGIARTLAIAKSADASLGPYFSKDVTASSKMSSEYQKQIQTLLSTPEEKALFDKIGEQ 122 + I RT + KS D L + +K+ ASSK S++ K ++ LL++ EEK +F I Sbjct: 61 LLIAIQRTTTVVKSHDPELEAFLAKEAAASSKESAQTLKDVEQLLASEEEKKMFAAINVA 120 Query: 123 RKIYLSSRDEIIKLKAAGNVEEAMRVLDKVFVPNAAVFQQLMRQLVDIQRKEMDESAMQI 182 RK +L+ RD+I K G+ + + K +V A Q MR L+D +R +D+ + QI Sbjct: 121 RKQFLTIRDQIYAAKKQGDAAQVEELYQKQYVGIAKSTQDAMRALLDFERARIDQISAQI 180 Query: 183 DAISAQSHIIILVLEGLILLLGIVLARILTLGITKPLQNAVVVSRKVAAGDLSASVQVHS 242 ++ S I++LE +ILL GI A +LT IT+P+ A+ +S++VAAGDL++ Q Sbjct: 181 QQDASSSQHQIMLLEVVILLCGIAFAVLLTRSITRPVHVALAISQRVAAGDLTSRAQAMG 240 Query: 243 QDETGQLLQALKDMNDSLRGIVSNVRSGTDTISTASSEIATGNLDLSSRTEEQAGSLAET 302 +DE GQL+ +L+ M++ L G+VS VR G D+I+ AS EIA GNLDLS+RTEEQAG+L +T Sbjct: 241 RDELGQLVASLQGMSERLHGVVSTVRHGADSIAMASGEIANGNLDLSARTEEQAGALEQT 300 Query: 303 ASAMEQLISTVRQNADNARQASQLAQSASSVAEQGGGVVSRVVDTMGAINASSRKIVDII 362 A+++E+L S+VR N+DNA QA++L +A+SVA QGG V+ +++ TM IN SSRKIVDII Sbjct: 301 AASIEELTSSVRTNSDNAHQANELVSTAASVAGQGGAVMDQLISTMATINDSSRKIVDII 360 Query: 363 GVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSASAAKEIKELIHDSV 422 GVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVR+LAQRSA+AAKEIK+LI DSV Sbjct: 361 GVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRALAQRSAAAAKEIKQLIDDSV 420 Query: 423 AKVGDGSRLVEQAGSTMQEVVSSVRHVTDIVAEISAASAEQTNGIEQINLAITQMDQVTQ 482 KV GS LVEQAG TMQEVV SV+ +V EIS AS +Q GI Q+N A+ QMD VTQ Sbjct: 421 GKVSAGSTLVEQAGKTMQEVVGSVQGAHTLVGEISTASRQQAEGIHQVNQAVGQMDGVTQ 480 Query: 483 QNAALVEQAAAAAASMQDQAGRLAQMVSIFRLH 515 QNAALVEQAAAAA S++DQA +L + V+ F L Sbjct: 481 QNAALVEQAAAAAKSLEDQAAQLKRAVAFFSLQ 513 >gi|300312038|ref|YP_003776130.1| methyl-accepting chemotaxis transmembrane I protein [Herbaspirillum seropedicae SmR1] Length = 579 Score = 446 bits (1146), Expect = e-126, Method: Compositional matrix adjust. Identities = 282/530 (53%), Positives = 356/530 (67%), Gaps = 28/530 (5%) Query: 1 MNILGNMNIGKRLTLGFAVILAFSAVVAAISLWRLEQVASSTREMMNAPLKTERLVADWY 60 M ++ I ++L F V++A A + S+ +L+QV S++ E+ +W Sbjct: 1 MKWFYDLRISRKLITTFLVVVAMVAALGVFSIRQLDQVNSASTEIST----------NWL 50 Query: 61 TNVNAGIARTLAIAKSADASLGPYFSKDVTASSKMSSEYQKQIQTL----------LSTP 110 ++ LA+++ L +KD ++ Q Q+ L +S P Sbjct: 51 PSIRTLARLQLAMSRLRSFELQHILAKDAKEYEEVERGMQVQMNNLKTAQAEYVTQISEP 110 Query: 111 EEKALFDKIGEQRKIYLSSRDEIIKLKAAGNVEEAMRVLDKVFVPNAAVFQQLMRQLVDI 170 EEKA++ ++ + ++S D+II L G EEA + Q + +L + Sbjct: 111 EEKAIYPEVEKLIATFVSEHDKIIALSRQGKDEEARNLQRGESTKAYRTSLQQIDKLAAV 170 Query: 171 QRKEMDESAMQIDAISAQSHIIILV----LEGLILLLGIVLARILTLGITKPLQNAVVVS 226 K D S Q DAI AQ+ + I+V GL +LL + ++R+ I +PL+ AV V+ Sbjct: 171 NDKGADISNKQADAIYAQARLWIIVTVAACIGLAMLLAVWVSRL----IARPLEEAVSVA 226 Query: 227 RKVAAGDLSASVQVHSQDETGQLLQALKDMNDSLRGIVSNVRSGTDTISTASSEIATGNL 286 ++VA GDL+ ++ +DETGQLL ALK MNDSL GIV VR GT TI+TAS+EIA+GNL Sbjct: 227 QRVAGGDLTTRIEPAGKDETGQLLVALKSMNDSLVGIVGQVRMGTQTITTASTEIASGNL 286 Query: 287 DLSSRTEEQAGSLAETASAMEQLISTVRQNADNARQASQLAQSASSVAEQGGGVVSRVVD 346 DLSSRTEEQA SL ETASAME+L STV+QNADNARQA+QLA SAS VA QGG VV++VVD Sbjct: 287 DLSSRTEEQASSLEETASAMEELTSTVKQNADNARQANQLAVSASEVASQGGAVVNQVVD 346 Query: 347 TMGAINASSRKIVDIIGVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQR 406 TMG+INASSRKI DIIGVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQR Sbjct: 347 TMGSINASSRKIADIIGVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQR 406 Query: 407 SASAAKEIKELIHDSVAKVGDGSRLVEQAGSTMQEVVSSVRHVTDIVAEISAASAEQTNG 466 SA AAKEIKELI SVA+V GS+LVE+AG TM EVV SVR VTDIV EIS+AS EQ+ G Sbjct: 407 SAGAAKEIKELIEGSVAQVDMGSKLVERAGITMSEVVQSVRRVTDIVGEISSASQEQSEG 466 Query: 467 IEQINLAITQMDQVTQQNAALVEQAAAAAASMQDQAGRLAQMVSIFRLHE 516 IEQ+NLAI QMD+VTQQNAALVE+AAAAA S+Q+QA L Q V F+L + Sbjct: 467 IEQVNLAIAQMDEVTQQNAALVEEAAAAAQSLQEQAETLTQTVGFFKLEQ 516 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.314 0.126 0.322 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,073,850 Number of extensions: 32686 Number of successful extensions: 502 Number of sequences better than 1.0e-08: 70 Number of HSP's gapped: 261 Number of HSP's successfully gapped: 71 Length of query: 536 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 446 Effective length of database: 1,193,720 Effective search space: 532399120 Effective search space used: 532399120 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 133 (55.8 bits)