BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2990.p (541 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312326|ref|YP_003776418.1| methyl-accepting chemotaxis pro... 930 0.0 gi|300313024|ref|YP_003777116.1| methyl-accepting chemotaxis pro... 140 3e-34 gi|300312336|ref|YP_003776428.1| methyl-accepting chemotaxis tra... 137 2e-33 gi|300313049|ref|YP_003777141.1| methyl-accepting chemotaxis I p... 132 8e-32 gi|300310055|ref|YP_003774147.1| methyl-accepting chemotaxis tra... 131 2e-31 >gi|300312326|ref|YP_003776418.1| methyl-accepting chemotaxis protein [Herbaspirillum seropedicae SmR1] Length = 541 Score = 930 bits (2403), Expect = 0.0, Method: Compositional matrix adjust. Identities = 494/541 (91%), Positives = 523/541 (96%) Query: 1 MKNWTIKQRLLGSFGLILLLMALMAGISALNLGRIDKSATALRRDSTPGLYHSTQINAAW 60 MKNWTIKQR+LGSF LILLLMALMAGISA+NLGRI+ ATALR+DSTPGLY+ST INAAW Sbjct: 1 MKNWTIKQRILGSFALILLLMALMAGISAVNLGRIENGATALRQDSTPGLYYSTMINAAW 60 Query: 61 YENLLLTQQIAQIDTNDAERQANLALLRRNAERLDKLQEDYDQTIFSDTDRNLFEQFKQV 120 YENLL++QQIAQIDTN+AERQA+LALLRRN ERLD+LQEDYD+TIFSD+DR LF+QFK+ Sbjct: 61 YENLLMSQQIAQIDTNEAERQADLALLRRNGERLDRLQEDYDKTIFSDSDRKLFDQFKET 120 Query: 121 RQRYRALVGEVLKMDFSQRPPIDKLFNEQIRPQWRAGYQLTQRMVDTNKAIADKAASRIN 180 RQ+YRA++ EVLKMDFSQR PIDK+FNEQIRPQWR GYQLTQRMVDTNK+IAD AASRIN Sbjct: 121 RQKYRAMISEVLKMDFSQRAPIDKMFNEQIRPQWRQGYQLTQRMVDTNKSIADDAASRIN 180 Query: 181 EAVTAAQFAMMTALMAALLAAMVCGFFLLRAINEPLQKLMGILDVMRSGDFSRRVALDRN 240 +AVT+AQFAM+++LMAALLAA+VCGFFL+RAINEPLQKLMGILD MRSGDFSRRVALDRN Sbjct: 181 DAVTSAQFAMLSSLMAALLAALVCGFFLMRAINEPLQKLMGILDYMRSGDFSRRVALDRN 240 Query: 241 DEFGVLGSGFDRMTDELAALVSQAQKSSARVATSVTEIAATARQQQATAAETAATTTEIG 300 DEFG LGSGFDRMTDEL ALVSQAQKSSARVATSVTEIAATARQQQATAAETAATTTEIG Sbjct: 241 DEFGHLGSGFDRMTDELTALVSQAQKSSARVATSVTEIAATARQQQATAAETAATTTEIG 300 Query: 301 ATSREIHATSRDLVRTMNEVSVVSEQTSALAGNGQAGLTRMGETMHNVMDAAGSVSAKLA 360 ATSREIH+TSRDLVRTMNEVSVVSEQTSALAGNGQAGLTRM +TM NVMDAAGSVSAKLA Sbjct: 301 ATSREIHSTSRDLVRTMNEVSVVSEQTSALAGNGQAGLTRMEDTMQNVMDAAGSVSAKLA 360 Query: 361 ILNEKAGNINQVVTTITKVADQTNLLSLNAAIEAEKAGEHGRGFSVVATEIRRLADQTAV 420 ILNEKAGNINQVVTTITKVADQTNLLSLNAAIEAEKAGEHGRGFSVVATEIRRLADQTAV Sbjct: 361 ILNEKAGNINQVVTTITKVADQTNLLSLNAAIEAEKAGEHGRGFSVVATEIRRLADQTAV 420 Query: 421 ATYDIEVMVKEIQSAVAAGVMGMDKFSDQVRRAMQDVQTAGSQLSQIIAQVQALAPRFQI 480 ATYDIEVMVKEIQSAVAAGVMGMDKFSDQVRR MQDVQTAGSQLSQIIAQVQALAPRFQI Sbjct: 421 ATYDIEVMVKEIQSAVAAGVMGMDKFSDQVRRGMQDVQTAGSQLSQIIAQVQALAPRFQI 480 Query: 481 VNEGMQAQANSAEQINQALVQLSEAAQQTVESLQQSSQAIEELNVVSNELRNGVSRFKVS 540 VNEGMQAQANSAEQINQALVQLSEAAQQTVESLQQSSQAIEEL+VVSNELRNGVSRFKV Sbjct: 481 VNEGMQAQANSAEQINQALVQLSEAAQQTVESLQQSSQAIEELHVVSNELRNGVSRFKVQ 540 Query: 541 D 541 D Sbjct: 541 D 541 >gi|300313024|ref|YP_003777116.1| methyl-accepting chemotaxis protein [Herbaspirillum seropedicae SmR1] Length = 571 Score = 140 bits (352), Expect = 3e-34, Method: Compositional matrix adjust. Identities = 135/529 (25%), Positives = 251/529 (47%), Gaps = 70/529 (13%) Query: 3 NW----TIKQRLLGSFGLILLLMALMAGISALNLGRIDKSATALRRDSTPGLYHSTQINA 58 NW I +L +F ++L+ + + G S+L L ++KS+T + R+ P + ++++ Sbjct: 2 NWFYNLKIAHKLNAAFLVVLVFIIGLGGFSSLELAAVNKSSTDIARNWLPSIDALSKMSL 61 Query: 59 AWYENLLL-TQQIAQIDTND-------AERQANLALLRRNAERLDKL-QEDYDQTIFSDT 109 A + QQ+ D + A++Q +A L + R KL E ++ ++ + Sbjct: 62 ALARSRSFDMQQLLTADKQEEKASRERADKQ--MATLLKEVARYGKLVSEPREKELYPEI 119 Query: 110 DRNLFEQFKQVRQRYRALVGEVLKMDFSQRPPIDKLFNEQIRPQWRAGYQLTQRMVDTNK 169 +RN+ +K+ +R AL + Q+ L N P +R + + + N Sbjct: 120 ERNI-ALYKEAHERMSAL------FEQDQKQAAHALMNSTSTPVYRKLQEQIGELSEVNS 172 Query: 170 ---AIADKAASRINEAVTAAQFAMMTALMAALLAAMVCGFFLLRAINEPLQKLMGILDVM 226 A++D A I A A++ + ++ AM +++ R + PL + I + Sbjct: 173 KGAALSDADADHI---YGQANMAILALVTTCVVLAMALSWWIARLVARPLANAVDIARQV 229 Query: 227 RSGDFSRRVALDRNDEFGVLGSGFDRMTDELAALVSQAQK-------SSARVATSVTEIA 279 +GD + + DE G L +M L +V + ++ +SA +AT +++ Sbjct: 230 AAGDLTADIPTAHRDETGQLLEALRQMNGNLLNIVREVRQGTDTITTASAEIATGNMDLS 289 Query: 280 ATARQQQATAAETAATTTEIGATSREIHATSRDLVRTMNEVSVVSEQTSALAGNGQAGLT 339 + QQ ETA+ E+ +T R+ + D R N+++V + + + QAG T Sbjct: 290 SRTEQQAGALEETASAMEELTSTVRQ----NSDNARQANQLAVNASEVA------QAGGT 339 Query: 340 RMGETMHNVMDAAGSVSAKLAILNEKAGNINQVVTTITKVADQTNLLSLNAAIEAEKAGE 399 +G+ V A ++ +N+ + I ++ I +A QTN+L+LNAA+EA +AGE Sbjct: 340 VVGK-----------VVATMSAINDSSRKIVDIIGVIDGIAFQTNILALNAAVEAARAGE 388 Query: 400 HGRGFSVVATEIRRLADQTAVATYDIEVMVKEIQSAVAAGVMGMDKFSDQVRRAMQDVQT 459 GRGF+VVA+E+R LA ++A A ++I+ ++ + + V G K D+ + M +V Sbjct: 389 QGRGFAVVASEVRTLAQRSAAAAHEIKALIDDSVAKVDTG----SKLVDEAGQTMNEVVA 444 Query: 460 AGSQLSQIIAQVQALAPRFQIVNEGMQAQANSAEQINQALVQLSEAAQQ 508 + ++S I+A++ A Q EQIN A+VQ+ E QQ Sbjct: 445 SVKRVSDIVAEITAAGAE----------QTQGIEQINNAVVQMDENTQQ 483 >gi|300312336|ref|YP_003776428.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 536 Score = 137 bits (345), Expect = 2e-33, Method: Compositional matrix adjust. Identities = 146/562 (25%), Positives = 259/562 (46%), Gaps = 74/562 (13%) Query: 3 NWTIKQRLLGSFGLILLLMALMAGISALNLGRIDKSATALRRDSTPGLYHSTQINAAWYE 62 N I +RL F LIL ++AGI L R+D A A R L + ++ A WY Sbjct: 6 NMNIGRRLTLGFALILTFSIIIAGI---GLWRLDHVAQATREMMAEPL-KTERLMADWYT 61 Query: 63 NLL--LTQQIAQIDTNDAERQANLAL-LRRNAERLDKLQEDYDQTIFSDTDRNLFEQFKQ 119 NL+ + + IA ++D A + +++ + Q+ + + + ++ LF++ Sbjct: 62 NLVAGIRRTIAIAKSSDPALGPYFAAEVAASSKSSGEYQKQVEALLSTPEEKALFQK--- 118 Query: 120 VRQRYRALVGEVLKMDFSQRPPIDK------------LFNEQIRPQWRAGYQLTQR-MVD 166 +GE+ K+ S R I+K + ++ P A YQ T R +VD Sbjct: 119 --------IGELRKLYLSSRDEINKAKAAGNTEEAMRILDQVFVPNATA-YQDTMRKLVD 169 Query: 167 TNKAIADKAASRINEAVTAAQFAMMTALMAALLAAMVCGFFLLRAINEPLQKLMGILDVM 226 + D A I+ ++ ++ LL +V +L I +PL + + + + Sbjct: 170 FQRKEMDDTAREIDAIAAKSRTLILVLEGLILLLGIVFARYLTLGITKPLHRAVDVSRCV 229 Query: 227 RSGDFSRRVALDRNDEFGVLGSGFDRMTDELAALVSQAQKSSARVATSVTEIAAT----- 281 GD S +A+ DE G L M L +VS + + ++T+ +EIAA Sbjct: 230 AEGDLSSEIAVTSRDETGQLLQSLKDMNQSLRGIVSNVRSGTDTISTASSEIAAGNLDLS 289 Query: 282 --ARQQQATAAETAATTTEIGATSREIHATSRDLVRTMNEVSVVSEQTSALAGNGQAGLT 339 QQ ++ AETA+ E+ +T R+ +R + N S V+EQ Sbjct: 290 SRTEQQASSLAETASAMEELISTVRQNADNARQASQLANSASAVAEQG------------ 337 Query: 340 RMGETMHNVMDAAGSVSAKLAILNEKAGNINQVVTTITKVADQTNLLSLNAAIEAEKAGE 399 G + V+D G++ N + I +++ I +A QTN+L+LNAA+EA +AGE Sbjct: 338 --GGVVSQVVDTMGAI-------NASSRKIVDIISVIDGIAFQTNILALNAAVEAARAGE 388 Query: 400 HGRGFSVVATEIRRLADQTAVATYDIEVMVKEIQSAVAAGVMGMDKFSDQVRRAMQDVQT 459 GRGF+VVA+E+R LA ++A A +I+ ++ + V G + +Q MQ+V + Sbjct: 389 QGRGFAVVASEVRTLAQRSASAAKEIKELINDSVGKVDDG----SRLVEQAGNTMQEVVS 444 Query: 460 AGSQLSQIIAQVQALAPRFQIVNEGMQAQANSAEQINQALVQLSEAAQQTVESLQQSSQA 519 + +++ I+A++ A + Q + EQIN A+ Q+ + QQ +++++ A Sbjct: 445 SVRRVTDIVAEISAASTE----------QTHGIEQINLAITQMDQVTQQNAALVEEAAAA 494 Query: 520 IEELNVVSNELRNGVSRFKVSD 541 + + L VS F++S+ Sbjct: 495 AASMQTQAGRLMQMVSVFRLSE 516 >gi|300313049|ref|YP_003777141.1| methyl-accepting chemotaxis I protein [Herbaspirillum seropedicae SmR1] Length = 516 Score = 132 bits (332), Expect = 8e-32, Method: Compositional matrix adjust. Identities = 133/522 (25%), Positives = 252/522 (48%), Gaps = 55/522 (10%) Query: 1 MKNWTIKQRLLGSFGLILLLMALMAGISALNLGRIDKSATALRRDSTPGLYHSTQINAAW 60 KN I ++L +F +L+LMA++ S L ++ +++ + D P + + Sbjct: 4 FKNLKIARKLQLAFLAVLVLMAILGIFSISQLAKVKNASSEISTDWMPAQKKLGDLKL-Y 62 Query: 61 YENLLLTQQIAQIDTNDAERQANLALLRRNA---ERLDKLQEDYDQTIFSDTDRNLFE-- 115 + L T+ +Q+ D + +A L+ R+ LDK +DY+ ++ + +R L+ Sbjct: 63 FVRLRGTE--SQLAIYDGDVEAMNGLVSRSQAILTGLDKTMKDYEASLDNVPERELYTKV 120 Query: 116 --QFKQVRQRYRALVGEVLKMDFSQRPPIDKLFNEQIRPQWRAGYQLTQRMVDTNKAIAD 173 Q + ++ +V L D Q + ++ ++ PQ + +++ N+ + Sbjct: 121 AGQIGEFLAEHKKIVAAFLARDNPQGRDLFLGYSNKLYPQILGDLE---KLIKINQDGGN 177 Query: 174 KAASRINEAVTAAQFAMMTALMAALLAAMVCGFFLLRAINEPLQKLMGILDVMRSGDFSR 233 A + ++ + ++ ++ L AAL M ++ R I PLQ + I + GD Sbjct: 178 AATADADDTFSKSRVGVLVLLAAALAIGMGLALYVARIIARPLQDAVDIARRVAEGDLGA 237 Query: 234 RVALDRNDEFGVLGSGFDRMTDELAALVSQAQKSSARVATSVTEIA------ATARQQQA 287 ++ + DE G L D M + L +V Q + + +AT+ TEIA + +QQA Sbjct: 238 QIQVQSKDETGQLMQALDTMNEGLRKVVGQVRSGTDSIATASTEIATGNLDLSRRTEQQA 297 Query: 288 TA-AETAATTTEIGATSREIHATSRDLVRTMNEVSVVSEQTSALAGNGQAGLTRMGETMH 346 TA ETA+ E+ +T ++ + D R N++++ + S +A G + ++ ETM Sbjct: 298 TALEETASALEELTSTVKQ----NTDNARQANQLAMAA---SGVAARGGGVVGQVVETME 350 Query: 347 NVMDAAGSVSAKLAILNEKAGNINQVVTTITKVADQTNLLSLNAAIEAEKAGEHGRGFSV 406 + NE + I ++++ I +A QTN+L+LNAA+EA +AGE GRGF+V Sbjct: 351 AI--------------NEASNRIVEIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAV 396 Query: 407 VATEIRRLADQTAVATYDIEVMVKEIQSAVAAGVMGMDKFSDQVRRAMQDVQTAGSQLSQ 466 VA+E+R LA ++A A +I+ ++ + S V AG V+ AGS +S+ Sbjct: 397 VASEVRSLAQRSASAAKEIKQLIDDSVSKVGAGT--------------SLVRQAGSTMSE 442 Query: 467 IIAQVQALAPRFQIVNEGMQAQANSAEQINQALVQLSEAAQQ 508 ++ V+ + ++ Q+ ++IN+A+ Q+ E QQ Sbjct: 443 VVESVKRVTDVVSEISAASTEQSVGIDEINRAVTQMDEVTQQ 484 >gi|300310055|ref|YP_003774147.1| methyl-accepting chemotaxis transducer transmembrane protein I [Herbaspirillum seropedicae SmR1] Length = 547 Score = 131 bits (329), Expect = 2e-31, Method: Compositional matrix adjust. Identities = 144/561 (25%), Positives = 267/561 (47%), Gaps = 66/561 (11%) Query: 1 MKNWTIKQRLLGSFGLILLLMALMAGISALNLGRIDKSATAL------RRDSTPGLYHST 54 MKN I RL F +L+L+ +A + R++ L + ++ + + Sbjct: 1 MKNLKIGHRLGLGFAAVLILLVAVAAFGLQQVSRLNDRIVFLTSVDEGKLEALSKVQFAV 60 Query: 55 QINAAWYENLLLTQQIAQIDTNDAERQANLALLRRNAERLD----KLQEDYDQTIFSDTD 110 + A NL L Q + A+++ +L L+ + + +D +L S + Sbjct: 61 GLRAIAARNLTLVQ-------SAADQKNDLDLVGSSQQDIDAGMAELGRLMQHPASSPQE 113 Query: 111 RNLFEQFKQVRQRYRALVGEVLKMDFSQRPPIDK--LFNEQIRPQWRAGYQLTQ--RMVD 166 R + E+ +Q+ +Y + ++K+ SQ+ + L + + R Q+ Q +++ Sbjct: 114 REMLEKLRQLEAQYLPVAVNIVKLATSQQTEAARASLVKDCMPILNRVIAQVEQFNKLLR 173 Query: 167 TNKAIADKAASRINEAVTAAQFAMMTALMAALLAAMVCGFFLLRAINEPLQKLMGILDVM 226 N ++ A T A++ M+ A +AAL + + L R+I+ PL + + + + Sbjct: 174 KN---SEANVEEAEAAYTLAKWTMLAASLAALTLGGMVAWLLTRSISRPLAQAVSVAQSV 230 Query: 227 RSGDFSRRVALDRNDEFGVLGSGFDRMTDELAALVSQAQKSSARVATSVTEI-------A 279 +SGD R+ + DE G L S M D L +V + ++ + +A + EI + Sbjct: 231 QSGDLGSRIEVTSKDELGQLMSALKGMNDSLVNIVGEVRQGTDTIAVASDEIKQGNMDLS 290 Query: 280 ATARQQQATAAETAATTTEIGATSREIHATSRDLVRTMNEVSVVSEQTSALAGNGQAGLT 339 QQ + ETA+ ++ AT ++ +R+ R +++ S +A G A + Sbjct: 291 NRTEQQAGSLQETASAMEQLTATVKQNADNAREANR-------LADNASQVAARGGAAVG 343 Query: 340 RMGETMHNVMDAAGSVSAKLAILNEKAGNINQVVTTITKVADQTNLLSLNAAIEAEKAGE 399 R+ +TM+ S+SA + I +++ I +A QTN+L+LNAA+EA +AGE Sbjct: 344 RVVDTMN-------SISA-------SSHKIVDIISVIDGIAFQTNILALNAAVEAARAGE 389 Query: 400 HGRGFSVVATEIRRLADQTAVATYDIEVMVKEIQSAVAAGVMGMDKFSDQVRRAMQDVQT 459 GRGF+VVA+E+R LA ++AVA +IE ++ E +VA V G + S+ Sbjct: 390 QGRGFAVVASEVRTLAQRSAVAAKEIEALINE---SVAKVVTGEKQVSE----------- 435 Query: 460 AGSQLSQIIAQVQALAPRFQIVNEGMQAQANSAEQINQALVQLSEAAQQTVESLQQSSQA 519 AGS + +++ ++++ ++ Q + EQIN+A+ Q+ EA QQ ++Q++ A Sbjct: 436 AGSTMHEVVESIKSVTHIMHDISAASAEQTSGLEQINRAVAQIDEATQQNAALVEQAAAA 495 Query: 520 IEELNVVSNELRNGVSRFKVS 540 + S L VS FK+S Sbjct: 496 AASMQQQSVALTEVVSIFKLS 516 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.127 0.334 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,153,457 Number of extensions: 37945 Number of successful extensions: 556 Number of sequences better than 1.0e-08: 68 Number of HSP's gapped: 296 Number of HSP's successfully gapped: 69 Length of query: 541 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 451 Effective length of database: 1,193,720 Effective search space: 538367720 Effective search space used: 538367720 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)