BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2988.p (419 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312324|ref|YP_003776416.1| methyltransferase of chemotaxis... 529 e-151 gi|300311336|ref|YP_003775428.1| chemotaxis protein methyltransf... 85 1e-17 gi|300311031|ref|YP_003775123.1| chemotaxis protein methyltransf... 80 4e-16 gi|300309960|ref|YP_003774052.1| chemotaxis protein methyltransf... 79 6e-16 gi|300312676|ref|YP_003776768.1| protein-glutamate methyltransfe... 65 8e-12 >gi|300312324|ref|YP_003776416.1| methyltransferase of chemotaxis proteins protein [Herbaspirillum seropedicae SmR1] Length = 433 Score = 529 bits (1362), Expect = e-151, Method: Compositional matrix adjust. Identities = 302/434 (69%), Positives = 340/434 (78%), Gaps = 16/434 (3%) Query: 1 MKTLPHIAELLKAAMGLDMATVGSSLIERVVRERMAALEITDDSLYLAVLQTSEAQLQAL 60 MK+L IA+ LKAAMGLDMATVG+SLIERVVRERMA LE+ DD+LYL++L +S A+LQAL Sbjct: 1 MKSLQAIADKLKAAMGLDMATVGASLIERVVRERMAVLEMEDDALYLSLLLSSAAELQAL 60 Query: 61 IESAVVPETWFFRDREAIFATARLARQRLAEQPAVPVRILSLPCSTGEEPYSLAMALQEE 120 IE AVVPETWFFRDREAI A ARLA +LA QPA +RILSLPCSTGEEPYSLAMAL + Sbjct: 61 IELAVVPETWFFRDREAILAVARLASDKLAAQPAAVIRILSLPCSTGEEPYSLAMALLDA 120 Query: 121 GVAPTQFVIDAYDIRTRSLEIAAAGVYGRNSFRGQDLGFRDRHFTPHEQGWQLQQEIRQQ 180 G+ P QFVIDAYDIRTRSLEIAAAG+YGRNSFRGQ+L FRDRHF + W+L + IR Q Sbjct: 121 GLTPGQFVIDAYDIRTRSLEIAAAGLYGRNSFRGQELAFRDRHFDLEQGQWRLHERIRSQ 180 Query: 181 VRFAPGNLLAPDFLRQAAPYDFIFCRNVLIYFEREVQQQVVDLLESLMKPDALVFVGPAE 240 VRFAPGNLLAPDFLR + PYDFIFCRNVLIYFER+VQ+QVV LL S+MKPDAL+FVGPAE Sbjct: 181 VRFAPGNLLAPDFLRHSQPYDFIFCRNVLIYFERDVQRQVVQLLASVMKPDALIFVGPAE 240 Query: 241 GGILLSPRMESAGIALAFGFRKRSSPLRPSRLPAWIT-----PPQVISAAPSTVCGAPRT 295 GGILL P MESAGIALAFGFRKR + P PAW + PP P AP Sbjct: 241 GGILLGPTMESAGIALAFGFRKRVAAAVPPLPPAWASLVQAAPPAPTPVRPDAAHRAPAR 300 Query: 296 GL----------TAVTTRPAMPQPTATAPTLLQQARVLADQGQFTQAEALCEQVMVEQGP 345 + TA RPA P TA LL++AR LADQG+F QA LCEQ + +QGP Sbjct: 301 RIVASAALGAVGTATAARPASP-ATALPAGLLERARALADQGRFAQAGELCEQAVQQQGP 359 Query: 346 SAEAFYLQGLIHDASGQDELAQRSYRKALYLQPQHQAALLQLAALLQAQGDAAGAERMRQ 405 SAEAFYLQGLIHDA GQ ELAQR YRKALYL+P+HQAALLQLAALLQAQGDA+GA+RMRQ Sbjct: 360 SAEAFYLQGLIHDAGGQQELAQRCYRKALYLEPEHQAALLQLAALLQAQGDASGAQRMRQ 419 Query: 406 RAARVSPVPEGSHG 419 RA R++ EG+HG Sbjct: 420 RAERMAKPGEGAHG 433 >gi|300311336|ref|YP_003775428.1| chemotaxis protein methyltransferase [Herbaspirillum seropedicae SmR1] Length = 290 Score = 84.7 bits (208), Expect = 1e-17, Method: Compositional matrix adjust. Identities = 75/247 (30%), Positives = 113/247 (45%), Gaps = 17/247 (6%) Query: 16 GLDMATVGSSLIERVVRERMAALEITDDSLYLAVLQTSE--AQLQALIESAVVPETWFFR 73 G+ +A ++ + R+ A IT YL +L+ + + +A + T FFR Sbjct: 35 GIALADSKQEMVYSRLARRLRATGITSFQQYLDMLERTHDSEEWEAFTNALTTNLTSFFR 94 Query: 74 DREAIFATARLARQRLAEQPAVPVRILSLPCSTGEEPYSLAM-ALQEEG--VAPTQFVID 130 + A + L E P I STGEEPY++AM A + G P Q V Sbjct: 95 EAHHFPILADFLKT-LKE----PATIWCSAASTGEEPYTIAMTACEAYGSLTPPVQIV-- 147 Query: 131 AYDIRTRSLEIAAAGVYGRNSFRGQD----LGFRDRHFTPHEQGWQLQQEIRQQVRFAPG 186 A DI T L + GVY F R HE +++ E+R+ + F Sbjct: 148 ATDIDTNVLATGSNGVYPMERIEKMSPERVKKFFLRGKGSHEGYVRVRPELRKLITFKQL 207 Query: 187 NLLAPDFLRQAAPYDFIFCRNVLIYFEREVQQQVVDLLESLMKPDALVFVGPAEGGILLS 246 NLLA D + +D IFCRNV+IYF++ Q +++ LMKP AL+F G +E + +S Sbjct: 208 NLLA-DGWPLSGQFDVIFCRNVMIYFDKPTQSKILSRFVPLMKPHALLFAGHSENFLYVS 266 Query: 247 PRMESAG 253 ++ G Sbjct: 267 DALKLKG 273 >gi|300311031|ref|YP_003775123.1| chemotaxis protein methyltransferase [Herbaspirillum seropedicae SmR1] Length = 280 Score = 79.7 bits (195), Expect = 4e-16, Method: Compositional matrix adjust. Identities = 60/239 (25%), Positives = 100/239 (41%), Gaps = 6/239 (2%) Query: 9 ELLKAAMGLDMATVGSSLIERVVRERMAALEITDDSLYLAVL--QTSEAQLQALIESAVV 66 E G+ +++ + ER+ A Y ++L + +LQ L V Sbjct: 16 EYFYRKTGISFEATKRYFVDKRLIERIEATGSGSFRHYFSLLRFEADGKELQLLTNLMTV 75 Query: 67 PETWFFRDREAI--FATARLARQRLAEQPAVPVRILSLPCSTGEEPYSLAMALQEE--GV 122 ET+F R+ + L + P+RI +PCS+GEE Y+LAM L E G+ Sbjct: 76 NETYFLREEYQFQCLVNSMLPEITRNRRSTAPIRIWCIPCSSGEEAYTLAMYLMERWPGI 135 Query: 123 APTQFVIDAYDIRTRSLEIAAAGVYGRNSFRGQDLGFRDRHFTPHEQGWQLQQEIRQQVR 182 I + DI T L A G Y S + + ++F +Q+ +R V Sbjct: 136 DQWDVEIISSDIDTGVLRKAQLGRYSARSVQHVPADWMSKYFLRQGDEYQICDALRGAVE 195 Query: 183 FAPGNLLAPDFLRQAAPYDFIFCRNVLIYFEREVQQQVVDLLESLMKPDALVFVGPAEG 241 F NL + +D IFCRN+LIYF+ +++ + + + + +G +E Sbjct: 196 FTRVNLAKSQDTKAYRNFDVIFCRNLLIYFDDVSRKEAAETFYEALNLNGFICLGHSES 254 >gi|300309960|ref|YP_003774052.1| chemotaxis protein methyltransferase [Herbaspirillum seropedicae SmR1] Length = 277 Score = 79.3 bits (194), Expect = 6e-16, Method: Compositional matrix adjust. Identities = 81/257 (31%), Positives = 126/257 (49%), Gaps = 20/257 (7%) Query: 10 LLKAAMGLDMATVGSSLIERVVRERMAALEITDDSLYLAVLQTSEA---QLQALIESAVV 66 L K +GL + +L++ + R+ L + Y A++ +SEA + Q I+ Sbjct: 24 LFKQQIGLHLPLSKKALLQSRLAGRLNELGLRHLGQYHALI-SSEAGAQERQRAIDLITT 82 Query: 67 PETWFFRDREAI-FATARLARQRLAEQPAVPVRILSLPCSTGEEPYSLAMALQEEGVAPT 125 ET+FFR+ F + Q LA+QP + RI S +TGEE Y+LAM L + Sbjct: 83 NETYFFREPGHFDFVVGQWLPQ-LAQQPQL--RIWSAASATGEEAYTLAMLLDHHRPGAS 139 Query: 126 QFVIDAYDIRTRSLEIAAAGVYGRNSFRGQDL--GFRDRH----FTPHEQGWQLQQEIRQ 179 V A DI TR L A +Y RGQ++ + R+ +E + +Q ++R Sbjct: 140 WSVF-ASDISTRVLRFARRALY--PMVRGQNIPRAYLRRYCLMGVGEYEGHFLVQADLRA 196 Query: 180 QVRFAPGNLLAPDFLRQAAPYDFIFCRNVLIYFEREVQQQVVDLLESLMKPDALVFVGPA 239 +V+FA NL D YD IF RNV+IYF+ E + QV+ + + ++P + VG + Sbjct: 197 KVQFAQRNLTRLD-EHDDGQYDLIFLRNVIIYFDCETKLQVLRDVIARLRPGGYLVVGHS 255 Query: 240 EG--GILLSPRMESAGI 254 E G+ L M+S I Sbjct: 256 ESLHGMDLGLDMQSPSI 272 >gi|300312676|ref|YP_003776768.1| protein-glutamate methyltransferase chemotaxis response regulator protein [Herbaspirillum seropedicae SmR1] Length = 283 Score = 65.5 bits (158), Expect = 8e-12, Method: Compositional matrix adjust. Identities = 59/205 (28%), Positives = 89/205 (43%), Gaps = 19/205 (9%) Query: 60 LIESAVVPETWFFRDREAIFATARLARQRLAEQPAVPVRILSLPCSTGEEPYSLAMALQE 119 L+E V + FRD A L P++ + + CS+GEE YSLA+ L+E Sbjct: 74 LMEYLTVQVSEMFRDPSYFLALREKVVPLLRTYPSLKIWVAG--CSSGEEVYSLAILLRE 131 Query: 120 EGVAPTQFVIDAYDIRTRSLEIAAAGVYGRNSFRG-----QDLGFR---DRHFTPHEQGW 171 EG+ +I A DI +L+ A AGVY G Q G R ++T Sbjct: 132 EGLLERS-IIYATDINQSALKKAEAGVYDLERIAGFTNNHQKSGARTSLSDYYTAAYGAV 190 Query: 172 QLQQEIRQQVRFAPGNLLAPDFLRQAAPYDFIFCRNVLIYFEREVQQQVVDLLESLMKPD 231 + +R+ V F+ +L A + CRNVLIYF R++Q++ + L + Sbjct: 191 VFDKSLRKNVVFSDHSLATDSVF---AEMHLVSCRNVLIYFNRQLQERALGLFRESLVRK 247 Query: 232 ALVFVGPAEGGILLSPRMESAGIAL 256 + +G E S R S +A Sbjct: 248 GFLGLGSKE-----SLRFSSHAVAF 267 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.133 0.385 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,109,548 Number of extensions: 41124 Number of successful extensions: 161 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 156 Number of HSP's successfully gapped: 5 Length of query: 419 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 331 Effective length of database: 1,203,190 Effective search space: 398255890 Effective search space used: 398255890 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 132 (55.5 bits)