BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2986.p (763 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312322|ref|YP_003776414.1| two component hybrid sensor his... 1249 0.0 gi|300312286|ref|YP_003776378.1| signal-transducing histidine ki... 200 4e-52 gi|300312396|ref|YP_003776488.1| chemotaxis CheA protein [Herbas... 194 2e-50 gi|300311033|ref|YP_003775125.1| chemotaxis histidine kinase [He... 192 6e-50 gi|300309957|ref|YP_003774049.1| chemotaxis histidine kinase [He... 188 1e-48 >gi|300312322|ref|YP_003776414.1| two component hybrid sensor histidine kinase/response regulator protein [Herbaspirillum seropedicae SmR1] Length = 775 Score = 1249 bits (3232), Expect = 0.0, Method: Compositional matrix adjust. Identities = 647/775 (83%), Positives = 696/775 (89%), Gaps = 13/775 (1%) Query: 1 MSTPDLSQMSMLDLFRFEAESQIAQLNTSLLALEQKPDAAEHLEACMRAGHSLKGAARIV 60 MSTPDLSQMSMLDLFRFEAESQIAQLN SLLALEQ+P AA+HLEACMRAGHSLKGAARIV Sbjct: 1 MSTPDLSQMSMLDLFRFEAESQIAQLNASLLALEQQPTAADHLEACMRAGHSLKGAARIV 60 Query: 61 GLDTAVKVAHVLEDCFVLAQRGQLRLEKKHIDVLLRGADLLGRIAAPPDGDEAWADHAGS 120 GL+ AVKVAHVLEDCFVLAQ+G+L L+KKHIDVLLRGADLL RIAAPPDGDEAWADHAGS Sbjct: 61 GLEPAVKVAHVLEDCFVLAQQGRLLLQKKHIDVLLRGADLLARIAAPPDGDEAWADHAGS 120 Query: 121 AEVQAFLLALPAVMSGGEAAPWPVPAAVGPATPELNAEVT--------PAP-----TSSP 167 AEVQAFL+ALP+VMSGG+AAPWP + + P A P P P Sbjct: 121 AEVQAFLVALPSVMSGGDAAPWPAATSAATSAPAPAAAEPVVAAAAQLPEPELAEPAPVP 180 Query: 168 ASPLSGEAVALAAASRAVRVSADSLDRLLGLSGEALVESRRLKPFSANMLRMKRGQREAM 227 A+P++G +A A+ +RAVRVSADSLDRLLGL+GE+LVESR+LKPFSA+MLRMKR QREAM Sbjct: 181 AAPVAGAHLANASGARAVRVSADSLDRLLGLTGESLVESRQLKPFSASMLRMKRTQREAM 240 Query: 228 QALDLLQHQLSSNQFDELTHATLNELRSLMQQNQQLLGEQLNALDAFDRRSLNLSQRLYD 287 QALDLLQ +L++ Q DE ELR+LMQQNQQLLGEQL+ALDAFDRRS+NLSQRLYD Sbjct: 241 QALDLLQQKLATTQLDEFAQTAFAELRTLMQQNQQLLGEQLSALDAFDRRSVNLSQRLYD 300 Query: 288 EALACRMRPFADGTGGFARMVRDVAHMLGKSVRLDISGNDTQIDRDILEKLEAPIGHLLR 347 EALACRMRPFADGT G ARMVRDV + LGKSVRLDI GN TQIDRDILEKLEAPIGHLLR Sbjct: 301 EALACRMRPFADGTAGLARMVRDVGNTLGKSVRLDIVGNTTQIDRDILEKLEAPIGHLLR 360 Query: 348 NALDHGIEEAATRRAAGKPEQGVIRLEARHSAGMLLIEVADDGAGIDLARLRQAVVARGL 407 NA+DHGIE +A RRAAGKPEQGVIRLEARHSAGMLLIEVADDGAGID+ ++R AVVARGL Sbjct: 361 NAVDHGIESSAQRRAAGKPEQGVIRLEARHSAGMLLIEVADDGAGIDIEQVRSAVVARGL 420 Query: 408 SNQETVERLSESELLDFLLLPSFSLRDTVTEISGRGVGLDVVADMLKQVRGTIRISTRAG 467 SNQET ERLSE+ELL+FLLLPSFSLRDTVTEISGRGVGLDVVADMLKQVRGTIRI+T+AG Sbjct: 421 SNQETAERLSEAELLEFLLLPSFSLRDTVTEISGRGVGLDVVADMLKQVRGTIRITTQAG 480 Query: 468 QGSRFLLQLPLTLSVIRSLLVDIGGEPYAFPLAYVNRTLRLPAEALQTLEGYQHFHHEGR 527 QGSRFL+QLPLTLSVIRSLLVDI GEPYAFPLAYVNRTLRLPAEALQTLEGYQHF H GR Sbjct: 481 QGSRFLMQLPLTLSVIRSLLVDIAGEPYAFPLAYVNRTLRLPAEALQTLEGYQHFSHGGR 540 Query: 528 QVGLISAHQVLHKGQWRVRDGSVCVVVIGEQERSYGVAVDAFLGERTLVVQPLDARLGKI 587 QVGL+SAHQVL KGQWR RDGSVCVVVIGEQE +YG+AVDAFLGER LVVQPLD RLGKI Sbjct: 541 QVGLVSAHQVLQKGQWRSRDGSVCVVVIGEQEHTYGLAVDAFLGERMLVVQPLDPRLGKI 600 Query: 588 PDILAGALMEDGAPLLILDVADLLRTVEKLTSSGRLQTVDQEGSSPATVVARKKVLVVDD 647 D+LAGALMEDG PLLILDVAD+LRTVEKLTSSGRLQTVDQ+G++P T ARKKVLVVDD Sbjct: 601 ADVLAGALMEDGTPLLILDVADMLRTVEKLTSSGRLQTVDQDGAAPTTTAARKKVLVVDD 660 Query: 648 SLTVRELERKLLTHRGYQVTVAVDGMDGWNAVRAEHFDLVITDIDMPRMDGIELVSLIRG 707 SLTVRELERKLLT+RGYQVTVAVDGMDGWNAVRA+ FDLVITDIDMPRMDGIELVSLIRG Sbjct: 661 SLTVRELERKLLTNRGYQVTVAVDGMDGWNAVRADSFDLVITDIDMPRMDGIELVSLIRG 720 Query: 708 APQWQSLPVMIVSYKDREEDRQRGLAAGADHYFTKSSFHDESLLRAVTDLIGEAT 762 A QSLPVMIVSYKDREEDRQRGL AGADHYFTKSSFHDESLL+AVTDLIGEAT Sbjct: 721 AANLQSLPVMIVSYKDREEDRQRGLEAGADHYFTKSSFHDESLLQAVTDLIGEAT 775 >gi|300312286|ref|YP_003776378.1| signal-transducing histidine kinase CheA protein [Herbaspirillum seropedicae SmR1] Length = 697 Score = 200 bits (508), Expect = 4e-52, Method: Compositional matrix adjust. Identities = 134/439 (30%), Positives = 225/439 (51%), Gaps = 53/439 (12%) Query: 182 SRAVRVSADSLDRLLGLSGEALVESRRLKPFSANMLRMKRGQREAMQALDLLQHQLSSNQ 241 S ++RVS + +D+L+ L GE ++ + M+ + G+ Sbjct: 304 SSSIRVSVEKVDQLINLVGELVI--------TQAMIEQRVGK------------------ 337 Query: 242 FDELTHATLNELRSLMQQNQQLLGEQLNALDAFDRRSLNLSQRLYDEALACRMRPFADGT 301 D +TH L LN++ R + +L + + ++ RM P Sbjct: 338 LDPMTHEHL-----------------LNSVSQLSRNTRDLQESV----MSIRMMPMDYVF 376 Query: 302 GGFARMVRDVAHMLGKSVRLDISGNDTQIDRDILEKLEAPIGHLLRNALDHGIEEAATRR 361 F RMVRD+AH LGK V G T++D+ ++E++ P+ HL+RN++DHGIE RR Sbjct: 377 SRFPRMVRDLAHKLGKQVEFVTHGAATELDKGLIERIVDPLTHLVRNSIDHGIELPDARR 436 Query: 362 AAGKPEQGVIRLEARHSAGMLLIEVADDGAGIDLARLRQAVVARGLSNQETVERLSESEL 421 A+GK E G + L A H G ++IEV DDG G++ ++ +G+ + LS++E+ Sbjct: 437 ASGKSEAGTLSLSAEHQGGNIIIEVTDDGGGLNREKILAKAKQQGMP---VTDNLSDAEV 493 Query: 422 LDFLLLPSFSLRDTVTEISGRGVGLDVVADMLKQVRGTIRISTRAGQGSRFLLQLPLTLS 481 + P FS + VT++SGRGVG+DVV + + G + I + G G+ + LPLTL+ Sbjct: 494 WQLIFEPGFSTAEQVTDVSGRGVGMDVVKRNILAMGGAVDIRSSKGFGTTISISLPLTLA 553 Query: 482 VIRSLLVDIGGEPYAFPLAYVNRTLRLPAEALQTLEGY-QHFHHEGRQVGLISAHQVLHK 540 ++ + + IG E Y PL YV +L+ A+ ++ + G Q G + LI +Q+ + Sbjct: 554 ILDGMSIRIGEEVYILPLGYVVESLQPLAQDIKEISGQGQVLRVRGEYLPLIPLYQIFNI 613 Query: 541 GQWRVRDGSVCVVVIGEQE-RSYGVAVDAFLGERTLVVQPLDARLGKIPDILAGALMEDG 599 + D S +VVI E + + + VD +G++ +VV+ L++ K+ I ++ DG Sbjct: 614 -EPSFTDPSQGLVVILESDGKKAALFVDDLIGQQQIVVKNLESNYRKVAGISGATILGDG 672 Query: 600 APLLILDVADLLRTVEKLT 618 LI+DVA LLR+ +L Sbjct: 673 GVALIVDVAALLRSSRQLN 691 >gi|300312396|ref|YP_003776488.1| chemotaxis CheA protein [Herbaspirillum seropedicae SmR1] Length = 638 Score = 194 bits (493), Expect = 2e-50, Method: Compositional matrix adjust. Identities = 138/403 (34%), Positives = 207/403 (51%), Gaps = 49/403 (12%) Query: 180 AASRAVRVSADSLDRLLGLSGEALVESRRLKPFSANMLRMKRGQREAMQALDLLQHQLSS 239 A ++R+ A LD LL ++GE++ + A QA LL+ +L Sbjct: 246 AKEESIRIDAGKLDALLEVAGESV--------------------QAANQAAVLLE-KLQQ 284 Query: 240 NQFDELTHATLNELRSLMQQNQQLLGEQLNALDAFDRRSLNLSQRLYDEALACRMRPFAD 299 +F+ A LM + LG R+ S L L+ RM+P Sbjct: 285 FKFEGTAAA-------LMSTLAETLG-----------RASRYSSELQRATLSTRMQPVGR 326 Query: 300 GTGGFARMVRDVAHMLGKSVRLDISGNDTQIDRDILEKLEAPIGHLLRNALDHGIEEAAT 359 F R+VR++A LGK V L I+G +T++DR +++ L P+ H+LRN+LDHGIE Sbjct: 327 LFQKFPRLVRELAKDLGKDVDLVITGAETEVDRVVVDSLYDPLVHMLRNSLDHGIE--VD 384 Query: 360 RRAAGKPEQGVIRLEARHSAGMLLIEVADDGAGIDLARLRQAVVARGLSNQETVERLSES 419 R AAGKP + I L A + ++IEV DDG G+D LR +A+GL N+ + L+ Sbjct: 385 RVAAGKPTKATISLNAWQAGSSVMIEVFDDGKGMDPDMLRSKALAKGLINEH--QALTAD 442 Query: 420 ELLDFLLLPSFSLRDTVTEISGRGVGLDVVADMLKQVRGTIRISTRAGQGSRFLLQLPLT 479 E L + LP FS ++ + +SGRGVG+DVV +++ RGTIRI + G+G++F ++LP+ Sbjct: 443 EALQLVFLPGFSTKEVASSVSGRGVGMDVVKTAVEKHRGTIRIESTIGKGTKFSIRLPIE 502 Query: 480 LSVIRSLLVDIGGEPYAFPLAYVNRTLRLPAEALQTLEGYQHFHHEGRQVGLISAHQVLH 539 LS+I ++LV G P A P+A V R + LP E + G +GR + + S VL Sbjct: 503 LSIIPTMLVRCAGAPLALPMAVVERVVELP-ENFDEVGGAPVLRDQGRPLPVNSLAGVLG 561 Query: 540 KGQWRVRDGSVCVVVIGEQERSYGVAVDAFLGERTLVVQPLDA 582 R G V V Y + V++ G LV++PL A Sbjct: 562 YEPGNERVGIVMAV-----PHPYILGVESVEGTADLVIKPLTA 599 >gi|300311033|ref|YP_003775125.1| chemotaxis histidine kinase [Herbaspirillum seropedicae SmR1] Length = 837 Score = 192 bits (489), Expect = 6e-50, Method: Compositional matrix adjust. Identities = 124/434 (28%), Positives = 227/434 (52%), Gaps = 44/434 (10%) Query: 180 AASRAVRVSADSLDRLLGLSGEALVESRRLKPFSANMLRMKRGQREAMQALDLLQHQLSS 239 + +++V+V +DRL+ L GE +V S+ P+ A + G RE + + Sbjct: 446 SGNKSVKVEQVKIDRLMNLIGEMVV-SKNALPYLAQRAEDQFGVRELAREI--------- 495 Query: 240 NQFDELTHATLNELRSLMQQNQQLLGEQLNALDAFDRRSLNLSQRLYDEALACRMRPFAD 299 + +A +N + MQ D + RM P + Sbjct: 496 ----KAQYAVINRIAEEMQ----------------------------DAIMQVRMMPVSV 523 Query: 300 GTGGFARMVRDVAHMLGKSVRLDISGNDTQIDRDILEKLEAPIGHLLRNALDHGIEEAAT 359 F R+VRD++ LGK V+L + G +T+ D++I+E L P+ H++RN+LDHG+E Sbjct: 524 VFQRFPRLVRDISRKLGKEVQLVLVGEETEADKNIIEALADPLIHIVRNSLDHGLEGPEQ 583 Query: 360 RRAAGKPEQGVIRLEARHSAGMLLIEVADDGAGIDLARLRQAVVARGLSNQETVERLSES 419 RRA+GKP G + + A H ++I + DDG GID A +++ +GL ++ +ER+S+ Sbjct: 584 RRASGKPALGSLTISAAHEGDQVVIVIEDDGKGIDPALIKRKAYEKGLIDEAQLERISDQ 643 Query: 420 ELLDFLLLPSFSLRDTVTEISGRGVGLDVVADMLKQVRGTIRISTRAGQGSRFLLQLPLT 479 + ++ + FS + V+++SGRGVG+DVV + +++V G++ + + +G+G+R + LPL+ Sbjct: 644 DAVNLVFSSGFSTAEVVSDLSGRGVGMDVVRNAVEKVNGSLALHSVSGKGTRLQISLPLS 703 Query: 480 LSVIRSLLVDIGGEPYAFPLAYVNRTLRLPAEALQTLEGYQHFHHEGRQVGLISAHQVLH 539 ++V + ++++ G+ + P+ +V T+R+P +L G +GL +Q+L Sbjct: 704 MAVTKVMIIESDGQLFGVPMEHVMETVRVPRSSLHFANSSAKMVLRGHILGLKGINQILG 763 Query: 540 KG--QWRVRDGSVCVVVIGEQERSYGVAVDAFLGERTLVVQPLDARLGKIPDILAGALME 597 Q DG V V+V+ +S G+ +D F ++ +PL LG + ALM Sbjct: 764 IAAEQKTNADGEVAVLVVRVGGQSVGLVIDDFRETVDVIQKPLLGILGGLSLYSGSALMG 823 Query: 598 DGAPLLILDVADLL 611 DG+ L++L+ ++ Sbjct: 824 DGSVLMVLNTREMF 837 >gi|300309957|ref|YP_003774049.1| chemotaxis histidine kinase [Herbaspirillum seropedicae SmR1] Length = 725 Score = 188 bits (478), Expect = 1e-48, Method: Compositional matrix adjust. Identities = 120/355 (33%), Positives = 192/355 (54%), Gaps = 9/355 (2%) Query: 280 NLSQRLYDEALACRMRPFADGTGGFARMVRDVAHMLGKSVRLDISGNDTQIDRDILEKLE 339 L +++ D AL RM + F R+VRD+A LGK + L ++G+DT++D+ ++E+L Sbjct: 379 TLMEQIRDAALTLRMVQINEVFQRFPRIVRDIARDLGKDIDLVMTGSDTELDKSMVERLA 438 Query: 340 APIGHLLRNALDHGIEEAATRRAAGKPEQGVIRLEARHSAGMLLIEVADDGAGIDLARLR 399 P+ H++RNA+DHGIE AA R AAGKP + ++L A H +G ++IEV DDG G+D + Sbjct: 439 DPLMHIVRNAIDHGIEPAAERLAAGKPAKASLQLNAIHESGSVVIEVMDDGRGMDREAIL 498 Query: 400 QAVVARGLSNQETVERLSESELLDFLLLPSFSLRDTVTEISGRGVGLDVVADMLKQVRGT 459 VA+GL++ + LS++E+ F+ P FS VTE+SGRGVG+DVV ++ + G Sbjct: 499 AKAVAQGLASPDA--ELSDAEIYRFVFEPGFSTARQVTELSGRGVGMDVVRRNIEALHGE 556 Query: 460 IRISTRAGQGSRFLLQLPLTLSVIRSLLVDIGGEPYAFPLAYVNRTLRLPAEALQTLEGY 519 + I T G+G+ L+LPLTL++I V +G + PL V + +P + G Sbjct: 557 VSIDTGRGRGTVVRLRLPLTLAIISGFQVVVGNAVFVIPLDMVVECIDMPPQQ----PGS 612 Query: 520 QHFHHEGRQVGLISAHQVLHKGQWRVRDGSVCVVVIGEQERSYGVAVDAFLGERTLVVQP 579 + + + ++ S+ +V G G+ VD LGE V++P Sbjct: 613 HIVNVRDEPLPFVPLRELFDLPARERGRNSLVIVQYGPLR--AGLLVDGLLGECQAVIKP 670 Query: 580 LDARLGKIPDILAGALMEDGAPLLILDVADLLRTVEKLTSSGRLQTVDQEGSSPA 634 L GK+ + ++ DG LILD+A L+ ++ G Q + G + A Sbjct: 671 LGRLFGKVKGLSGSTILGDGRVALILDIAHLVHHTQQ-QEQGSAQAYAERGVASA 724 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.135 0.378 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,144,402 Number of extensions: 89676 Number of successful extensions: 530 Number of sequences better than 1.0e-08: 35 Number of HSP's gapped: 517 Number of HSP's successfully gapped: 36 Length of query: 763 Length of database: 1,619,870 Length adjustment: 93 Effective length of query: 670 Effective length of database: 1,179,515 Effective search space: 790275050 Effective search space used: 790275050 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 134 (56.2 bits)