BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2985.p (340 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312321|ref|YP_003776413.1| protein-glutamate methylesteras... 544 e-156 gi|300311338|ref|YP_003775430.1| chemotaxis-specific methylester... 150 1e-37 gi|300311029|ref|YP_003775121.1| protein-glutamate methylesteras... 144 7e-36 gi|300309961|ref|YP_003774053.1| chemotaxis-specific methylester... 129 4e-31 gi|300312675|ref|YP_003776767.1| protein-glutamate methylesteras... 65 6e-12 >gi|300312321|ref|YP_003776413.1| protein-glutamate methylesterase [Herbaspirillum seropedicae SmR1] Length = 342 Score = 544 bits (1402), Expect = e-156, Method: Compositional matrix adjust. Identities = 275/341 (80%), Positives = 297/341 (87%), Gaps = 4/341 (1%) Query: 1 VKIGIVNDTPMMVEVLRRVIAESGRHELIWIARDGEQAVQMCAWQLPDVVLMDLLMPKMD 60 +KIGIVNDTPMMVEVLRRVIAESGRHELIWIARDGEQAVQMCAWQLPDVVLMDLLMPK+D Sbjct: 1 MKIGIVNDTPMMVEVLRRVIAESGRHELIWIARDGEQAVQMCAWQLPDVVLMDLLMPKVD 60 Query: 61 GVEATRIIMQETPCAILVVTSDMDTSVAKIYEAMGHGALDATQTPALIGAAGKRDAAALI 120 G EATR IM+ TPC ILVVTSDMDTS KIYEAMG+GALDATQTPALIGAAGKRDAAALI Sbjct: 61 GAEATRRIMEATPCPILVVTSDMDTSADKIYEAMGYGALDATQTPALIGAAGKRDAAALI 120 Query: 121 EKIDNLGRLSPEEMSAPVR----PCLAALIPAGQEDLAPLVAIGASAGGPAALATLLKQV 176 EKIDNLGRLSPE +S PVR LA PA + L PLVA+GASAGGPAALATLLK + Sbjct: 121 EKIDNLGRLSPEMVSPPVRGGRAETLAPAQPARADSLPPLVAVGASAGGPAALATLLKAI 180 Query: 177 PREFSGSIVIVQHIDASFAAGMAQWLQQQSTLRVRLAIEGDLPRRGEVLLAGSNRHLVFK 236 P+ F+G++VIVQHIDA+FA GMAQWLQQQS L+V LA EG P+RG VLLA SNRHLV K Sbjct: 181 PQGFAGAVVIVQHIDAAFAPGMAQWLQQQSALKVGLAQEGQAPQRGAVLLAASNRHLVVK 240 Query: 237 EGSRLGYRDGAPADVYRPSVDAFFHSIVRHWQGHATGVLLTGMGRDGAAGLKALRDNGYH 296 EG RLGY +GAP DVYRPSVD FF SIVRHW G G+LLTGMGRDGAAGLKALRD+G H Sbjct: 241 EGGRLGYVEGAPNDVYRPSVDVFFQSIVRHWPGKVAGLLLTGMGRDGAAGLKALRDSGAH 300 Query: 297 TLAQDQKSCAVYGMPKAAVALGAAVEVLPVSAMAARLQALL 337 T+AQDQKSCAVYGMPKAAVAL AAVEVLPV+ MAARLQA++ Sbjct: 301 TMAQDQKSCAVYGMPKAAVALNAAVEVLPVTGMAARLQAII 341 >gi|300311338|ref|YP_003775430.1| chemotaxis-specific methylesterase [Herbaspirillum seropedicae SmR1] Length = 359 Score = 150 bits (380), Expect = 1e-37, Method: Compositional matrix adjust. Identities = 108/351 (30%), Positives = 176/351 (50%), Gaps = 16/351 (4%) Query: 1 VKIGIVNDTPMMVEVLRRVIAESGRHELIWIARDGEQAVQMCAWQLPDVVLMDLLMPKMD 60 +K+ IV+D+ ++ V+R +I E++ +A D A ++ PDV+ +D+ MP+MD Sbjct: 3 IKVLIVDDSALIRSVMREIIGSQPDMEVVGVAPDPIIARELIKQTNPDVLTLDVEMPRMD 62 Query: 61 GVEATRIIMQETPCAILVVTSDMDTSVAKIYEAMGHGALDATQTPALIGAAGKRDAAALI 120 G++ +M+ P +++V+S + A+ GA+D P + +G ++ A +I Sbjct: 63 GLDFLEKLMRLRPMPVVMVSSLTERGSEITLRALELGAIDFVTKPKISIQSGMQEYADMI 122 Query: 121 EKIDNLGRLSPEEMSAPVRPCLAALIPAGQEDL----------APLVAIGASAGGPAALA 170 D + + + A G E L L+ IGAS GG A+ Sbjct: 123 A--DKIRAAAKARVRARPARSAEQAAGGGSEALPQIRNPLTSSEKLIIIGASTGGTEAIK 180 Query: 171 TLLKQVPREFSGSIVIVQHIDASFAAGMAQWLQQQSTLRVRLAIEGDLPRRGEVLLAGSN 230 L ++P + G I+I QH+ F AQ L + V + + G +A + Sbjct: 181 EFLIRMPSDCPG-ILITQHMPEGFTRSFAQRLNNLCKISVVESAGNERVLPGHAYIAPGH 239 Query: 231 RHL-VFKEGSRLGYR--DGAPADVYRPSVDAFFHSIVRHWQGHATGVLLTGMGRDGAAGL 287 HL + + G+ + G P + +RPSVD F S +A GV+LTGMG+DGA G+ Sbjct: 240 SHLKLVRSGANYMTQLDQGPPVNRHRPSVDVLFQSAATCAGKNAVGVILTGMGKDGAQGM 299 Query: 288 KALRDNGYHTLAQDQKSCAVYGMPKAAVALGAAVEVLPVSAMAARLQALLA 338 +R+ G + AQD+ SC V+GMP+ A+A+GAA EVL + AM R+ A LA Sbjct: 300 LEMRNAGAYNFAQDEASCVVFGMPREAIAVGAAHEVLALQAMPGRVLAWLA 350 >gi|300311029|ref|YP_003775121.1| protein-glutamate methylesterase [Herbaspirillum seropedicae SmR1] Length = 353 Score = 144 bits (364), Expect = 7e-36, Method: Compositional matrix adjust. Identities = 109/351 (31%), Positives = 176/351 (50%), Gaps = 15/351 (4%) Query: 1 VKIGIVNDTPMMVEVLRRVIAESGRHELIWIARDGEQAVQMCAWQLPDVVLMDLLMPKMD 60 +K+ I++D+ +M L +V ++G + I + R+G +AV++ PDVV +D+ MP+MD Sbjct: 2 IKLLIIDDSALMRRQLMQVFEQAGGFD-ICLGRNGREAVELNRSFAPDVVTLDINMPEMD 60 Query: 61 GVEATRIIMQETPCAILVVTSDMDTSVAKIYEAMGHGALDATQTPA-LIGAAGKRDAAAL 119 G+ A ++M + P +++V+S + +EAM GA+D P+ I + L Sbjct: 61 GLSALSLMMLDRPVPVVMVSSLTEQGALATFEAMNLGAVDYVAKPSGTISLSLHEIEQDL 120 Query: 120 IEKID-----NLGRLSPEEMSAPVRPCLAALIPAGQEDLAP----LVAIGASAGGPAALA 170 + K+ + +P A R LAA + P LV IG S GGP+ L Sbjct: 121 LAKVRVAAKARIRSRAPAPELARAREPLAAPVAEAARRGRPASDGLVLIGVSTGGPSTLE 180 Query: 171 TLLKQVPREFSGSIVIVQHIDASFAAGMAQWLQQQSTLRVRLAIEGDLPRRGEVLLA-GS 229 +L +P F I++ QH+ A+F A+ + + L V + + G V +A G Sbjct: 181 EILPALPASFPWPILVAQHMPAAFTRSFAERMDKLCALNVLEVAAPTVVQGGHVYIARGG 240 Query: 230 NRHLVFKEGSRLGY--RDGAPADVYRPSVDAFFHSIVRHWQG-HATGVLLTGMGRDGAAG 286 LV K GS L + +P ++ PSV+ S ++ GV+LTGMG DGAA Sbjct: 241 ADMLVAKRGSHLSVLPKPESPTHLWHPSVELLARSALQSMAARQLIGVMLTGMGNDGAAA 300 Query: 287 LKALRDNGYHTLAQDQKSCAVYGMPKAAVALGAAVEVLPVSAMAARLQALL 337 + +G T+A+ + SC V+GMPK + G A VLP S +A +L+ L Sbjct: 301 FAEICGDGGRTIAESESSCVVFGMPKDLIDRGGASVVLPASKIATQLRVWL 351 >gi|300309961|ref|YP_003774053.1| chemotaxis-specific methylesterase [Herbaspirillum seropedicae SmR1] Length = 381 Score = 129 bits (323), Expect = 4e-31, Method: Compositional matrix adjust. Identities = 114/364 (31%), Positives = 174/364 (47%), Gaps = 50/364 (13%) Query: 14 EVLRRVIAESGRHELIWIARDGEQAVQMCAWQLPDVVLMDLLMPKMDGVEATRIIMQETP 73 +++++V+A++ +I A D A++ Q PDV+++D+ MP+MDGV R IM P Sbjct: 20 QLMQQVLAQTPDIRVIGTAPDPVFALRKMQQQWPDVIVLDIKMPRMDGVSFLRQIMATRP 79 Query: 74 CAILVVTSDMDTSVAKIYEAMGHGALDATQTPALIGAAGKRDAAALIEKIDNLG------ 127 +++ ++ + A EA+ GA+ P L G RD L + ++ L Sbjct: 80 TPVIMCSAHVGPDNALAAEALAAGAVSVIARPQL----GVRDF--LQQAVEELASAIRAA 133 Query: 128 --------------RLSPEEMSAPVRPCL---------------AALIPAGQED-----L 153 RL + A R L A L P G ED Sbjct: 134 MQAREGQRAPKAPARLGLSPVKAGAREMLHDAAWGAVLQRYTADAVLQPPGPEDGLRPAT 193 Query: 154 APLVAIGASAGGPAALATLLKQVPREFSGSIVIVQHIDASFAAGMAQWLQQQSTLRVRLA 213 A +VAIGAS GGP AL +L ++ + G IV+VQH+ F A+ L + S V+ A Sbjct: 194 ARIVAIGASTGGPQALERVLGKLSQRCPG-IVVVQHMPQRFTRSFAERLHRISGAEVKEA 252 Query: 214 IEGDLPRRGEVLLAGSNRHLVFKEGSRLGY---RDGAPADVYRPSVDAFFHSIVRHWQGH 270 D G VL+A RHLV + Y DG ++PSVD F S+ + + Sbjct: 253 EHLDRILPGRVLIAPGGRHLVVRREGLQYYVETLDGPLVSRHKPSVDVLFRSLAKAAGSN 312 Query: 271 ATGVLLTGMGRDGAAGLKALRDNGYHTLAQDQKSCAVYGMPKAAVALGAAVEVLPVSAMA 330 A G+++TGMG DGA G++ L D G T AQD+ S V+GMPK A+ G +V+ + ++ Sbjct: 313 AVGIIMTGMGDDGARGMRELADCGGATYAQDEASSVVFGMPKEAIQQGGVGDVITLEHIS 372 Query: 331 ARLQ 334 A ++ Sbjct: 373 AVIE 376 >gi|300312675|ref|YP_003776767.1| protein-glutamate methylesterase [Herbaspirillum seropedicae SmR1] Length = 211 Score = 65.5 bits (158), Expect = 6e-12, Method: Compositional matrix adjust. Identities = 53/183 (28%), Positives = 89/183 (48%), Gaps = 3/183 (1%) Query: 157 VAIGASAGGPAALATLLKQVPREFSGSIVIVQHIDASFAAGMAQWLQQQSTLRVRLAIEG 216 + IGASAGG AL+ +L + R + + ++V H+ + +AQ + V A + Sbjct: 17 IVIGASAGGVEALSEILPALRRGAAVATMVVLHLPRERPSLLAQIFADRCRQPVVEAQDK 76 Query: 217 DLPRRGEVLLAGSNRHLVF---KEGSRLGYRDGAPADVYRPSVDAFFHSIVRHWQGHATG 273 + + G + A + HL+ ++G L + + RP++D F S + G Sbjct: 77 EPAQAGVIHFAPPDYHLLVDRSEDGPILAMSNDELVNYSRPAIDVLFESAADVYGPRLLG 136 Query: 274 VLLTGMGRDGAAGLKALRDNGYHTLAQDQKSCAVYGMPKAAVALGAAVEVLPVSAMAARL 333 +LLTG DGAAG++A+ G T+ Q + V MP A+A+G VL + +A L Sbjct: 137 ILLTGGNHDGAAGMQAIARAGGVTVVQSPQQAQVPYMPAQALAMGNIDYVLTLQQIADLL 196 Query: 334 QAL 336 + L Sbjct: 197 RTL 199 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.134 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,093,734 Number of extensions: 51155 Number of successful extensions: 295 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 296 Number of HSP's successfully gapped: 6 Length of query: 340 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 254 Effective length of database: 1,212,660 Effective search space: 308015640 Effective search space used: 308015640 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 131 (55.1 bits)