BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2980.p (385 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312316|ref|YP_003776408.1| pyridoxal phosphate dependent a... 754 0.0 gi|300312878|ref|YP_003776970.1| aspartate/tyrosine/aromatic ami... 115 7e-27 gi|300311875|ref|YP_003775967.1| aspartate/tyrosine/aromatic ami... 106 3e-24 gi|300311409|ref|YP_003775501.1| aspartate aminotransferase [Her... 87 2e-18 gi|300311478|ref|YP_003775570.1| succinyldiaminopimelate aminotr... 80 2e-16 >gi|300312316|ref|YP_003776408.1| pyridoxal phosphate dependent aspartate/tyrosine/aromatic aminotransferase [Herbaspirillum seropedicae SmR1] Length = 385 Score = 754 bits (1948), Expect = 0.0, Method: Compositional matrix adjust. Identities = 364/385 (94%), Positives = 371/385 (96%) Query: 1 MSTPNIVTKLPKVGTTIFTVMSALASEKGAVNLGQGFPDFHCDPALVAAVTQAMQDGLNQ 60 MSTPNIVTKLPKVGTTIFTVMSALASEKGAVNLGQGFPDFHCDPALVAAVTQAMQ+GLNQ Sbjct: 1 MSTPNIVTKLPKVGTTIFTVMSALASEKGAVNLGQGFPDFHCDPALVAAVTQAMQEGLNQ 60 Query: 61 YPPMAGVLPLRQAIAAKVERLYGHRYDPVSDITVTAGATQGILTSVLCAVHPGDEVIVIE 120 YPPMAGV LR+AIA KVE+LYGHRYDPVS+ITVTAGATQGILTSVLCAVHPGDEVIVIE Sbjct: 61 YPPMAGVPVLREAIAEKVEKLYGHRYDPVSEITVTAGATQGILTSVLCAVHPGDEVIVIE 120 Query: 121 PVYDCYVPAIELAGGVPVCVQMEVGEHGYSIPWHKVQAAVSAKTRMIMVNTPHNPTGSVM 180 PVYDCYVPAIELAGGVPV VQMEVG GYSIPW KV+AAV+ KTRMIMVNTPHNPTGSVM Sbjct: 121 PVYDCYVPAIELAGGVPVFVQMEVGAQGYSIPWDKVKAAVTPKTRMIMVNTPHNPTGSVM 180 Query: 181 RATDVAALADIVRGTDILILSDEVYEHMVYDGQPHESLARHPELAERSFINSSFGKTYHV 240 RA DVAALADIVRGTDILILSDEVYEHMVYDGQPHESLARHPELAERSFINSSFGKTYHV Sbjct: 181 RAADVAALADIVRGTDILILSDEVYEHMVYDGQPHESLARHPELAERSFINSSFGKTYHV 240 Query: 241 TGWKVGYVAAPAALTAEFRKVHQFNVFTVNTPVQYGLATYMKDPSPYLDLPAFYQRKRDL 300 TGWKVGYVAAPAALTAEFRKVHQFNVFTVNTPVQYGLA YMKDP+PYLDLPAFYQ KRDL Sbjct: 241 TGWKVGYVAAPAALTAEFRKVHQFNVFTVNTPVQYGLAAYMKDPAPYLDLPAFYQHKRDL 300 Query: 301 FRNGLANTRFELLPSQGTYFQCVKYSAISDLPEAEFCKWLTSEIGVAAIPVSAFYNTPRE 360 FR GLANTRFELLPSQGTYFQCVKY AIS LPEAEFCKWLTSEIGVAAIPVSAFYNTPRE Sbjct: 301 FRTGLANTRFELLPSQGTYFQCVKYGAISALPEAEFCKWLTSEIGVAAIPVSAFYNTPRE 360 Query: 361 SGIVRFCFAKKDETLQLALERLAKL 385 SGIVRFCFAKKDETLQLALERLAKL Sbjct: 361 SGIVRFCFAKKDETLQLALERLAKL 385 >gi|300312878|ref|YP_003776970.1| aspartate/tyrosine/aromatic aminotransferase [Herbaspirillum seropedicae SmR1] Length = 393 Score = 115 bits (287), Expect = 7e-27, Method: Compositional matrix adjust. Identities = 101/366 (27%), Positives = 176/366 (48%), Gaps = 33/366 (9%) Query: 31 VNLGQGFPDFHCDPALVAAVTQAMQDGLNQYPPMAGVLPLRQAIAAKVERLYGHRYDPVS 90 +++G G PDF PA+V A T+AM +G QY G+ LR AI+ +YG P Sbjct: 36 IHMGIGEPDFTAPPAVVEAATRAMTEGKMQYTSATGLPQLRAAISDHYRSVYGLEIAP-E 94 Query: 91 DITVTAGATQGILTSVLCAVHPGDEVIVIEPVYDC---YVPAIE-----LAGGVPVCVQM 142 I VTAGA+ +L + V EV++ +P Y C +V A E +A G Sbjct: 95 RIVVTAGASAALLLACAALVERDSEVLMPDPSYPCNRHFVAAFEGRAKLIASG------- 147 Query: 143 EVGEHGYSIPWHKVQAAVSAKTRMIMVNTPHNPTGSVMRATDVAALADIVRGTDILILSD 202 EH + + V+ + TR +++ +P NPTG+ + ++ A+ VR + D Sbjct: 148 --PEHRFQLSAQMVREHWGSATRGVLLASPSNPTGTSILPDELRAIVGEVRQRGGFTIVD 205 Query: 203 EVYEHMVYDGQPHESLARHPELAERSFINSSFGKTYHVTGWKVGYVAAPAALTAEFRKVH 262 E+Y+ + Y+G P +L+ L + + +SF K +++TGW++G++ P AL + K+ Sbjct: 206 EIYQGLSYEGAPFSALS----LGDDVVVINSFSKYFNMTGWRLGWLVLPPALVPQVEKLA 261 Query: 263 QFNVFTVNTPVQYGLATYM--KDPSPYLDLPAFYQRKRDLFRNGLANTRFE--LLPSQGT 318 Q + ++ Q+ + + Y + A ++R+RD L + F ++P G Sbjct: 262 QNLLICASSIAQHAAVACFTPQTLALYEERKAEFKRRRDYIVPALESLGFTVPVMPD-GA 320 Query: 319 YFQCVKYSAISDLPEAEFCKWLTSEIGVAAIPVSAF--YNTPRESGIVRFCFAKKDETLQ 376 ++ SA++D + + + +E GV +P F + R +R +A E LQ Sbjct: 321 FYVYADCSALADDAD-QLSLDMLNEAGVVLVPGLDFGPFTARR---YIRLSYATSMENLQ 376 Query: 377 LALERL 382 A+ RL Sbjct: 377 EAVARL 382 >gi|300311875|ref|YP_003775967.1| aspartate/tyrosine/aromatic aminotransferase [Herbaspirillum seropedicae SmR1] Length = 431 Score = 106 bits (264), Expect = 3e-24, Method: Compositional matrix adjust. Identities = 89/379 (23%), Positives = 162/379 (42%), Gaps = 8/379 (2%) Query: 7 VTKLPKVGTTIFTVMSALASEKGA--VNLGQGFPDFHCDPALVAAVTQ-AMQDGLNQYPP 63 + +LP I + A +G +++ G PD P +V + + A + + Y Sbjct: 44 IERLPPYVFNITAELKMAARRRGEDIIDMSMGNPDGATPPHIVEKLVEVAQRPDTHGYSS 103 Query: 64 MAGVLPLRQAIAAKVERLYGHRYDPVSDITVTAGATQGILTSVLCAVHPGDEVIVIEPVY 123 G+ LR+AIA Y +DP S+ VT G+ +G+ +L + GD V+V P Y Sbjct: 104 SKGIPRLRRAIAHWYRSRYEVDFDPDSEAIVTIGSKEGLAHLMLATLDKGDTVLVPNPSY 163 Query: 124 DCYVPAIELAGGVPVCVQMEVGEHGYSIPWHKVQAAVSAKTRMIMVNTPHNPTGSVMRAT 183 ++ +AG V+M G + V+ + K +M+++ P NPT + Sbjct: 164 PIHIYGAVIAGANIRSVRMSPGVDFFDELERAVRESYP-KPKMMILGFPSNPTAQCVELE 222 Query: 184 DVAALADIVRGTDILILSDEVYEHMVYDGQPHESLARHPELAERSFINSSFGKTYHVTGW 243 + + R IL++ D Y + +DG S+ + P E + + K+Y++ GW Sbjct: 223 FFERVVKLAREHQILVVHDLAYADITFDGWKAPSIMQVPGAREVAVEFFTLSKSYNMAGW 282 Query: 244 KVGYVAAPAALTAEFRKVHQFNVFTVNTPVQY-GLATYMKDPSPYLDLPAFYQRKRDLFR 302 ++G++ A L A ++ ++ + TPVQ +A D S ++ A Y+R+R++ Sbjct: 283 RIGFMVGNARLVAALARIKSYHDYGSFTPVQVAAIAALEGDQSCVEEIRANYERRRNVLV 342 Query: 303 NGLANTRFEL-LPSQGTYFQCVKYSAISDLPEAEFCKWLTSEIGVAAIPVSAFYNTPRES 361 GL + + +P Y EF + L + V P F E Sbjct: 343 KGLHEAGWMVDVPKASMYIWARIPEPYRQFGSLEFARILLEQAKVCVSPGIGFGEYGDE- 401 Query: 362 GIVRFCFAKKDETLQLALE 380 VRF + + ++ A+ Sbjct: 402 -YVRFALIENESRIRQAIR 419 >gi|300311409|ref|YP_003775501.1| aspartate aminotransferase [Herbaspirillum seropedicae SmR1] Length = 409 Score = 87.0 bits (214), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 56/203 (27%), Positives = 101/203 (49%), Gaps = 17/203 (8%) Query: 89 VSDITVTAGATQGILTSVLCAVHPGDEVIVIEPVYDCYVPAIELAGGVPV---CVQMEVG 145 + DI + GA++ I+ SV ++ GDEV+V P Y + A+ L+GG PV C + Sbjct: 95 IDDIYLGNGASELIVMSVNALLNTGDEVLVPSPDYPLWTAAVSLSGGTPVHYVCDE---- 150 Query: 146 EHGYSIPWHKVQAAVSAKTRMIMVNTPHNPTGSVMRATDVAALADIVRGTDILILSDEVY 205 + G+ ++ ++ T+ I+V P+NPTG++ + + ++ R ++IL+DE+Y Sbjct: 151 QQGWQPDIADIKKKITPNTKAIVVINPNNPTGALYSVEVLKEIIELARQHQLIILADEIY 210 Query: 206 EHMVYDGQPHESLARHPELAERS-FIN-SSFGKTYHVTGWKVGYVAAPAALTAEFRKVHQ 263 + ++YDG H SLA LA+ FI + K Y G++ G++ + Sbjct: 211 DKVLYDGNTHTSLA---SLADDVLFITFNGLSKNYRSCGYRAGWMVVSGEKKHARDYIEG 267 Query: 264 FNVFT-----VNTPVQYGLATYM 281 N+ N P Q+ + T + Sbjct: 268 LNMLASMRLCANAPGQFAIQTAL 290 >gi|300311478|ref|YP_003775570.1| succinyldiaminopimelate aminotransferase [Herbaspirillum seropedicae SmR1] Length = 420 Score = 80.1 bits (196), Expect = 2e-16, Method: Compositional matrix adjust. Identities = 85/333 (25%), Positives = 141/333 (42%), Gaps = 17/333 (5%) Query: 31 VNLGQGFPDFHCDPALVAAVTQAMQD---GLNQYPPMAGVLPLRQAIAAKVERLYG-HRY 86 ++LG G P H PA + QA+ D GL YP G LR AIA +++ Y R Sbjct: 49 ISLGIGEPK-HPTPAFI---QQALIDNLAGLASYPATLGSDALRGAIADWLQKRYDIPRP 104 Query: 87 DPVSDITVTAGATQGILT---SVLCAVHPGDEVIVIEPVYDCYVPAIELAGGVPVCVQME 143 DP ++I G+ + + + +V+ PG V+ P Y Y + LAG P V + Sbjct: 105 DPATEILPVNGSREALFSLTQTVVDPTRPGALVMCPNPFYQIYEGSAYLAGAQPYFVNSD 164 Query: 144 VGEHGYSIPWHKVQAAVSAKTRMIMVNTPHNPTGSVMRATDVAALADIVRGTDILILSDE 203 + ++ + +V V + +++ V +P NPTG+V+ D L + +I SDE Sbjct: 165 -PKRNFAPDFSQVPQDVWERVQLLFVCSPGNPTGAVLTLEDWKELFALSDRYGFVIASDE 223 Query: 204 VYEHMVYDGQPHESLARHPELAERSFIN----SSFGKTYHVTGWKVGYVAAPAALTAEFR 259 Y + ++ P + L R + SS K +V G + G+VA AA+ +F Sbjct: 224 CYSEIYFNEDPPLGGMQAARLLGRDYRRLISFSSLSKRSNVPGMRSGFVAGDAAILKKFL 283 Query: 260 KVHQFNVFTVNTPVQYGLATYMKDPSPYLDLPAFYQRKRDLFRNGLANTRFELLPSQGTY 319 ++ ++ +Q +D + A Y K L LP Y Sbjct: 284 LYRTYHGAAMSPVIQAASVLAWQDETHVQGNIAKYVTKFSQVTPLLQEVLEVALPDAAFY 343 Query: 320 FQCVKYSAISDLPEAEFCKWLTSEIGVAAIPVS 352 K + ++ + EF + L +E V +P S Sbjct: 344 L-WAKVDKLVNISDTEFAQRLYAEYNVTVLPGS 375 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.135 0.408 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,243,078 Number of extensions: 53751 Number of successful extensions: 162 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 157 Number of HSP's successfully gapped: 6 Length of query: 385 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 298 Effective length of database: 1,207,925 Effective search space: 359961650 Effective search space used: 359961650 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 131 (55.1 bits)