BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2969.p (246 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312302|ref|YP_003776394.1| acetoacetyl-CoA reductase [Herb... 489 e-140 gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein... 189 1e-49 gi|300313009|ref|YP_003777101.1| 3-oxoacyl-(acyl-carrier-protein... 163 1e-41 gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein... 141 5e-35 gi|300310426|ref|YP_003774518.1| gluconate 5-dehydrogenase oxido... 138 5e-34 >gi|300312302|ref|YP_003776394.1| acetoacetyl-CoA reductase [Herbaspirillum seropedicae SmR1] Length = 246 Score = 489 bits (1260), Expect = e-140, Method: Compositional matrix adjust. Identities = 238/246 (96%), Positives = 241/246 (97%) Query: 1 MSKRIAYVTGGMGGIGTAICTRLCKDGYTVVAGCGPNSTRKDTWLAAMRAHGYDIHASEG 60 MSKRIAYVTGGMGGIGTAICTRLCKDGYTVVAGCGPNSTRKD WLA MR G+DIHASEG Sbjct: 1 MSKRIAYVTGGMGGIGTAICTRLCKDGYTVVAGCGPNSTRKDKWLATMREQGFDIHASEG 60 Query: 61 NVSDWESTKAAFEKVRAEIGEVDVLVNNAGITRDGQFRKMSKADWDAVIDTNLNSLFNVT 120 NVS+WESTKAAFEKVRAEIGEVDVLVNNAGITRDGQFRKMSK DWDAVIDTNLNSLFNVT Sbjct: 61 NVSNWESTKAAFEKVRAEIGEVDVLVNNAGITRDGQFRKMSKDDWDAVIDTNLNSLFNVT 120 Query: 121 KQVIEGMCERGFGRIINISSVNGQKGQFGQTNYSTAKAGIHGFTMALAQEVATKGVTVNT 180 KQVIEGMCERGFGRIINISSVNGQKGQFGQTNYSTAKAGIHGFTMALAQEVATKGVTVNT Sbjct: 121 KQVIEGMCERGFGRIINISSVNGQKGQFGQTNYSTAKAGIHGFTMALAQEVATKGVTVNT 180 Query: 181 VSPGYIGTDMVRAIRPEVLEKIVSGIPVKRLGEPEEIASIVAWIASAEGGYATGSDFSLN 240 VSPGYIGTDMVRAIRPEVLEKIVSGIPVKRLGEPEEIASIVAWIAS+EGGYATGSDFSLN Sbjct: 181 VSPGYIGTDMVRAIRPEVLEKIVSGIPVKRLGEPEEIASIVAWIASSEGGYATGSDFSLN 240 Query: 241 GGLYMG 246 GGLYMG Sbjct: 241 GGLYMG 246 >gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein] reductase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 189 bits (481), Expect = 1e-49, Method: Compositional matrix adjust. Identities = 105/251 (41%), Positives = 154/251 (61%), Gaps = 10/251 (3%) Query: 1 MSKRIAYVTGGMGGIGTAICTRLCKDGYTVVAGCGPNS--TRKDTWLAAMRAHGYDIHAS 58 + ++A VTG GIG AI T L + G V+ S +LAA+ A Sbjct: 6 LQGQVALVTGASRGIGRAIATELARQGAKVIGTATSESGAAAITEYLAAL-----GPEAG 60 Query: 59 EG---NVSDWESTKAAFEKVRAEIGEVDVLVNNAGITRDGQFRKMSKADWDAVIDTNLNS 115 G NV+D E++ A E+V+ + G + +LVNNAGIT+D +M +WD+VI TNL + Sbjct: 61 RGAVLNVNDAEASVALVEQVQKDFGSLSILVNNAGITQDQLAMRMKDEEWDSVIATNLTA 120 Query: 116 LFNVTKQVIEGMCERGFGRIINISSVNGQKGQFGQTNYSTAKAGIHGFTMALAQEVATKG 175 + +++ V+ GM + GRIINI+SV G+ G GQ NY+ AKAG+ G + ALA+E+ ++ Sbjct: 121 VGRLSRAVLRGMMKARTGRIINITSVVGEAGNPGQMNYAAAKAGVAGMSRALAREIGSRN 180 Query: 176 VTVNTVSPGYIGTDMVRAIRPEVLEKIVSGIPVKRLGEPEEIASIVAWIASAEGGYATGS 235 +TVN V+PG+I TDM RA+ + I+ IP+ RLG EEIA VA++AS + Y TG+ Sbjct: 181 ITVNCVAPGFIDTDMTRALNEQQSAAILQQIPLGRLGAAEEIAYAVAFLASPQAAYITGT 240 Query: 236 DFSLNGGLYMG 246 ++NGG+YMG Sbjct: 241 TLNVNGGMYMG 251 >gi|300313009|ref|YP_003777101.1| 3-oxoacyl-(acyl-carrier-protein) reductase [Herbaspirillum seropedicae SmR1] Length = 245 Score = 163 bits (413), Expect = 1e-41, Method: Compositional matrix adjust. Identities = 94/238 (39%), Positives = 133/238 (55%), Gaps = 4/238 (1%) Query: 7 YVTGGMGGIGTAICTRLCKDGYTVVAGCGPNSTRKDTWLAAMRAHGYDIHASEGNVSDWE 66 +VTG GIG AI RL ++GY +V C D A + A G + + +V++ E Sbjct: 10 FVTGSSRGIGRAIALRLAREGYDIVLHCHARRDAADQVAAEIAALGRQVRVLQFDVANRE 69 Query: 67 STKAAFEKVRAEIGEVDVLVNNAGITRDGQFRKMSKADWDAVIDTNLNSLFNVTKQVIEG 126 + G +V NAGITRD F M DWD+V+ TNL+ +NV ++ Sbjct: 70 QAAQVLLADVEQHGCYYGVVCNAGITRDNAFPAMPGEDWDSVLRTNLDGFYNVLNPLVMP 129 Query: 127 MCER-GFGRIINISSVNGQKGQFGQTNYSTAKAGIHGFTMALAQEVATKGVTVNTVSPGY 185 M R GRI+ ++SV+G G GQ NYS AKAG+ G T ALA E+A + +TVN V+PG Sbjct: 130 MVRRRAAGRIVTLASVSGLIGNRGQVNYSAAKAGVIGATKALALELAKRDITVNCVAPGL 189 Query: 186 IGTDMVRAIRPEVLEKIVSGIPVKRLGEPEEIASIVAWIASAEGGYATGSDFSLNGGL 243 I TDM +++I+ IP +R G+PEE+A+ VA++ S + Y T S+NGGL Sbjct: 190 IDTDMT---EDAPIDEILKLIPARRAGKPEEVAAAVAFLMSPDAAYITRQVISVNGGL 244 >gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein) reductase [Herbaspirillum seropedicae SmR1] Length = 256 Score = 141 bits (355), Expect = 5e-35, Method: Compositional matrix adjust. Identities = 86/245 (35%), Positives = 133/245 (54%), Gaps = 4/245 (1%) Query: 4 RIAYVTGGMGGIGTAICTRLCKDGYTV-VAGCGPNSTRKDTWLAAMRAHGYDIHASEGNV 62 R+A VTGG GIG +I +L G+TV VA ++ K A+ A G+ + ++ Sbjct: 9 RVALVTGGAMGIGASISQQLAAKGHTVLVADINLDAASKTA--DALVAAGHRAAPLQMDL 66 Query: 63 SDWESTKAAFEKVRAEIGEVDVLVNNAGITRDGQFRKMSKADWDAVIDTNLNSLFNVTKQ 122 ES AAF + + G DVLVNNAG+ R F +W ++ N+ + + Sbjct: 67 GSPESIAAAFAHIEKDYGRCDVLVNNAGVARTYSFLDYPLENWLQTMNVNVTGVLLAGQH 126 Query: 123 VIEGMCERGFGRIINISSVNGQKGQFGQTNYSTAKAGIHGFTMALAQEVATKGVTVNTVS 182 M ++ +GRI+NISS++G + G+T Y T+KA + G T +A E+A G+TVN+V+ Sbjct: 127 AARLMVKQQWGRIVNISSISGIRAGAGRTAYGTSKAAVIGLTRQMAIELAQYGITVNSVA 186 Query: 183 PGYIGTDMVRAIRPE-VLEKIVSGIPVKRLGEPEEIASIVAWIASAEGGYATGSDFSLNG 241 PG + T M +A+ E E +P++R G +EIAS V ++A+ E Y TG ++G Sbjct: 187 PGPVDTPMTQAMHSEQTRESYYRLVPMRRYGTTDEIASAVCYLAAEESAYITGHVIPVDG 246 Query: 242 GLYMG 246 G G Sbjct: 247 GFVAG 251 >gi|300310426|ref|YP_003774518.1| gluconate 5-dehydrogenase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 264 Score = 138 bits (347), Expect = 5e-34, Method: Compositional matrix adjust. Identities = 80/245 (32%), Positives = 130/245 (53%), Gaps = 3/245 (1%) Query: 1 MSKRIAYVTGGMGGIGTAICTRLCKDGYTVVAGCGPNSTRKDTWLAAMRAHGYDIHASEG 60 + ++A +TG GGIG A+ + + G +V G ++ + A +RA G+ IH S Sbjct: 17 LEGKLALITGSSGGIGLALARGMAQAGAAIVLN-GRDAGKLAAVAAQLRAEGHTIHESSF 75 Query: 61 NVSDWESTKAAFEKVRAEIGEVDVLVNNAGITRDGQFRKMSKADWDAVIDTNLNSLFNVT 120 + + E KAA +K+ +EIG + +LVNNAGI R + ++DW +I TNLNS+F Sbjct: 76 DATSSEEVKAAVQKIESEIGPIGILVNNAGIQRRNPLEEFKESDWHDLISTNLNSVFFAA 135 Query: 121 KQVIEGMCERGFGRIINISSVNGQKGQFGQTNYSTAKAGIHGFTMALAQEVATKGVTVNT 180 + V M RG G+IINI SV + G+ G Y+ K + FT +A + G+ VN Sbjct: 136 QAVARHMIPRGAGKIINICSVQSELGRPGIAPYTATKGAVKMFTKGMAIDWGKYGIQVNG 195 Query: 181 VSPGYIGTDMVRAI--RPEVLEKIVSGIPVKRLGEPEEIASIVAWIASAEGGYATGSDFS 238 + PGY T+M A+ + + +V P +R G+ E++ ++AS + G Sbjct: 196 LGPGYFKTEMNMALVNDAKFTDWLVGRTPSRRWGDVEDLVGAAVFLASDASRFVNGHILY 255 Query: 239 LNGGL 243 ++GG+ Sbjct: 256 VDGGV 260 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.133 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 813,430 Number of extensions: 32511 Number of successful extensions: 252 Number of sequences better than 1.0e-08: 48 Number of HSP's gapped: 162 Number of HSP's successfully gapped: 48 Length of query: 246 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 163 Effective length of database: 1,226,865 Effective search space: 199978995 Effective search space used: 199978995 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 129 (54.3 bits)