BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2966.p (144 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312295|ref|YP_003776387.1| signal peptide protein [Herbasp... 233 4e-63 >gi|300312295|ref|YP_003776387.1| signal peptide protein [Herbaspirillum seropedicae SmR1] Length = 143 Score = 233 bits (595), Expect = 4e-63, Method: Compositional matrix adjust. Identities = 121/143 (84%), Positives = 130/143 (90%) Query: 2 MAGPMMRRGLLAAALVLCTAVAWSPAQAASSGPAANAPPGSLQQTLMTHTLVLRGQIDGR 61 M M+RRGLLAAAL L +A A +P++AASSGPAANA GSLQQTLMTH LVLRGQIDGR Sbjct: 1 MGSLMLRRGLLAAALALVSATAPAPSEAASSGPAANAAEGSLQQTLMTHALVLRGQIDGR 60 Query: 62 DIQLSLAPKKNEDGLEGRYFFFGGSPEILVAGEVDGDDLIMEESINGKDVSGQWEGHRQG 121 +IQLSL PKKNEDGLEGRYFFFGGSPEILVAGEVDGDDLIMEES+NGKDVSGQWEG RQG Sbjct: 61 EIQLSLQPKKNEDGLEGRYFFFGGSPEILVAGEVDGDDLIMEESVNGKDVSGQWEGRRQG 120 Query: 122 QSIVGTWSSADGAISKPFALQLP 144 QSI GTWSSADGA++KPFALQLP Sbjct: 121 QSITGTWSSADGAVTKPFALQLP 143 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.134 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 388,803 Number of extensions: 15354 Number of successful extensions: 50 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 50 Number of HSP's successfully gapped: 1 Length of query: 144 Length of database: 1,619,870 Length adjustment: 77 Effective length of query: 67 Effective length of database: 1,255,275 Effective search space: 84103425 Effective search space used: 84103425 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 126 (53.1 bits)