BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2965.p (401 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312294|ref|YP_003776386.1| cystathionine beta-lyase [Herba... 749 0.0 gi|300312963|ref|YP_003777055.1| o-acetylhomoserine sulfhydrylas... 128 1e-30 gi|300312582|ref|YP_003776674.1| O-acetylhomoserine sulfhydrylas... 103 2e-23 >gi|300312294|ref|YP_003776386.1| cystathionine beta-lyase [Herbaspirillum seropedicae SmR1] Length = 392 Score = 749 bits (1933), Expect = 0.0, Method: Compositional matrix adjust. Identities = 358/384 (93%), Positives = 375/384 (97%) Query: 18 LDPQTALIHSSYRAPEGFEAFPVGIHHASTVLFPNVAAMRSRDWREKLGYTYGLHGTPTS 77 L PQT+L+HSSYRAP+GFEAFPVGIHHASTVLFP+VAAMRSRDWREKLGYTYGLHGTPTS Sbjct: 9 LTPQTSLVHSSYRAPDGFEAFPVGIHHASTVLFPDVAAMRSRDWREKLGYTYGLHGTPTS 68 Query: 78 FVLEARLAEIEGGSHCRVTPSGLAAIAMVNFAFLKSGDDVLLPDNVYGPSKDLGQWLERD 137 FVLEARLAEIEGG +CRVTPSGLAAIAMVNFAFLKSGDDVLLPDNVYGPSKDLGQWLERD Sbjct: 69 FVLEARLAEIEGGKYCRVTPSGLAAIAMVNFAFLKSGDDVLLPDNVYGPSKDLGQWLERD 128 Query: 138 FQITARFYDPMIGAGLAELIQPNTRLIWTEAPGSVSMEVPDIPALCRAAHARGVLVAIDN 197 FQITARFYDPMIG GLAELI+P TRL+WTEAPGSVSMEVPDIPALCRAAHA+GVLVAIDN Sbjct: 129 FQITARFYDPMIGEGLAELIRPTTRLVWTEAPGSVSMEVPDIPALCRAAHAKGVLVAIDN 188 Query: 198 TWAAGLAFKAFDHGVDIVMQAVTKYQSGGSDVLMGAVIVRDQALNEKLELAHMRLGFGVG 257 TW+AG+AFKAF+HGVDIVMQAVTKYQSGGSDVLMGAVIVRDQALNEKLELAHMRLGFGVG Sbjct: 189 TWSAGIAFKAFEHGVDIVMQAVTKYQSGGSDVLMGAVIVRDQALNEKLELAHMRLGFGVG 248 Query: 258 MDDVYLVLRSLSSMRLRFQAHDAAARKVAAWLKQRPEIVAVLHPAFEDCPGHENWKRDFT 317 MDDVY+VLRSLSSMRLRF+AHDAAARKVAAWLKQRPEIVAVLHPAFEDCPGH +WKRDF+ Sbjct: 249 MDDVYMVLRSLSSMRLRFEAHDAAARKVAAWLKQRPEIVAVLHPAFEDCPGHAHWKRDFS 308 Query: 318 GAGGLFSVIFDPRYGEQQTDRFVDSLRLFKIGYSWGGAHSLCVPYRMRGMRRQWQREGQL 377 GAGGLFSVIFDPRY E QTDRFVDSLRLFKIGYSWGGAHSLCVPYRM+GMRR W REGQL Sbjct: 309 GAGGLFSVIFDPRYVEAQTDRFVDSLRLFKIGYSWGGAHSLCVPYRMKGMRRAWMREGQL 368 Query: 378 VRFNIGLESPEDLIADIEQALAQL 401 VRFNIGLESPEDLIADIEQALA+L Sbjct: 369 VRFNIGLESPEDLIADIEQALARL 392 >gi|300312963|ref|YP_003777055.1| o-acetylhomoserine sulfhydrylase [Herbaspirillum seropedicae SmR1] Length = 429 Score = 128 bits (321), Expect = 1e-30, Method: Compositional matrix adjust. Identities = 108/374 (28%), Positives = 167/374 (44%), Gaps = 43/374 (11%) Query: 66 GYTYGLHGTPTSFVLEARLAEIEGGSHCRVTPSGLAAIAMVNFAFLKSGDDVLLPDNVYG 125 G Y PT VLE RLA +EGG SG AA+ ++GD+++ +YG Sbjct: 55 GNIYSRIMNPTQDVLEKRLAALEGGIGALALASGQAAVTYAIQTIAEAGDNIVSASTLYG 114 Query: 126 PSKDLGQWLERDFQITARFYDPMIGAGLAELIQPNTRLIWTEAPGSVSMEVPDIPALCRA 185 + +L + + RF DP A LI T+ I+ E+ G+ + DI A+ Sbjct: 115 GTYNLFAHTLPQYGVQTRFADPRQPASFEPLIDDRTKAIYIESIGNPLGNITDIAAVADI 174 Query: 186 AHARGVLVAIDNTWAAGLAFKAFDHGVDIVMQAVTKYQSGGSDVLMGAV----------- 234 AH GV + +DNT A + F+HG DIV+ ++TKY G L GA+ Sbjct: 175 AHRHGVPLIVDNTVATPYLLRPFEHGADIVVHSLTKYLGGHGTTLGGAIVDSGKFPWAKH 234 Query: 235 ----------------IVRDQALNEKLELAHMR------LGFGVGMDDVYLVLRSLSSMR 272 +V +AL E + R +G + + + +L+ + ++ Sbjct: 235 KQRFKRLNEPDVSYHGVVYTEALGEAAYIGRARVVPLRNMGAALSPFNAFQILQGIETLG 294 Query: 273 LRFQAHDAAARKVAAWLKQRPEIVAVLHPAFEDCPGHENWKRDFTG-AGGLFSVIFDPRY 331 LR A VA +LK+ P++ V + ED P H + F G A G+ + F Sbjct: 295 LRLDRITANTLAVAQYLKRHPKVRWVNYAGLEDHPDHALAQTYFKGRASGVLT--FGVAN 352 Query: 332 GEQQTDRFVDSLRLFKIGYSWGGAHSLCVPYRMRGMRR----QWQREG---QLVRFNIGL 384 G + RF D+L+LF + G A SL R+ + ++ G VR +IG+ Sbjct: 353 GREGGARFQDALQLFTRLVNIGDAKSLATHPASTTHRQLSPTELEKAGVTEDTVRLSIGI 412 Query: 385 ESPEDLIADIEQAL 398 E +DL+AD+EQAL Sbjct: 413 EHIDDLLADLEQAL 426 >gi|300312582|ref|YP_003776674.1| O-acetylhomoserine sulfhydrylase [Herbaspirillum seropedicae SmR1] Length = 413 Score = 103 bits (258), Expect = 2e-23, Method: Compositional matrix adjust. Identities = 103/409 (25%), Positives = 168/409 (41%), Gaps = 43/409 (10%) Query: 19 DPQTALIHSSYRAPEGFEAFPVGIHHASTVLFPNVAAMRSRDWREKLGYTYGLHGTPTSF 78 D Q + H S P IH + T + + + ++ GY YG G PT Sbjct: 17 DRQKGIEHGSLHKP---------IHTSVTFGYEDARQLAEVFQGKQPGYRYGRQGNPTVA 67 Query: 79 VLEARLAEIEGGSHCRVTPSGLAAIAMVNFAFLKSGDDVLLPDNVYGPSKDLGQWLERDF 138 LE ++ ++E G +G+AAI + L+ GD V+ ++G + L W+ Sbjct: 68 ALEDKITKMEDGKSTICFATGMAAIGAIVQGLLREGDHVVSSAFLFGNTNSL--WMTVGA 125 Query: 139 Q-ITARFYDPMIGAGLAELIQPNTRLIWTEAPGSVSMEVPDIPALCRAAHARGVLVAIDN 197 Q D + I NTRL++ E + +V D+ + RG+L +DN Sbjct: 126 QGAKVSMVDATDVKNVEAAITANTRLVFVETIANPRTQVADLKRIGELCRERGILYVVDN 185 Query: 198 TWAAGLAFKAFDHGVDIVMQAVTKYQSGGSDVLMGAVIVRDQ---------ALNEKLE-- 246 T + F+ G +V+ ++TK G + L GA+ + A N K Sbjct: 186 TMTSPYLFRPKTVGAGLVVNSLTKSIGGHGNALGGALTDTGEFDWTRYPHIAENYKKNPA 245 Query: 247 ----LAHMR------LGFGVGMDDVYLVLRSLSSMRLRFQAHDAAARKVAAWLKQRPEIV 296 +A +R G +G + + + ++ LR + A +A L+ + Sbjct: 246 PQWGMAQIRAKALRDFGGSLGPEAAHHIAVGAETIALRQERECKNALALAQMLQADERVA 305 Query: 297 AVLHPAFEDCPGHENWKRDFTGAGGLFSVIFDPRYGEQQTDRFVDSLRLFKIGYSWGGAH 356 AV +P E P H K F G L S F+ + G D +++ LRL + G Sbjct: 306 AVYYPGLESHPQHALSKALFRSFGSLMS--FELKDGIDCFD-YLNRLRLAIPTSNLGDTR 362 Query: 357 SLCVP------YRMRGMRRQWQREGQ-LVRFNIGLESPEDLIADIEQAL 398 +L +P Y M RR + L+R ++GLE +DL+AD QAL Sbjct: 363 TLVIPVAHTIFYEMGAERRASMGIAESLIRVSVGLEDTDDLVADFRQAL 411 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.138 0.426 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,346,483 Number of extensions: 58938 Number of successful extensions: 161 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 157 Number of HSP's successfully gapped: 3 Length of query: 401 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 314 Effective length of database: 1,207,925 Effective search space: 379288450 Effective search space used: 379288450 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 132 (55.5 bits)