BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2941.p (475 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312271|ref|YP_003776363.1| 2-oxoglutarate dehydrogenase E3... 952 0.0 gi|300310787|ref|YP_003774879.1| 2-oxoglutarate dehydrogenase E3... 308 4e-85 gi|300312608|ref|YP_003776700.1| glutathione reductase [Herbaspi... 167 2e-42 >gi|300312271|ref|YP_003776363.1| 2-oxoglutarate dehydrogenase E3 component lipoamide dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 475 Score = 952 bits (2461), Expect = 0.0, Method: Compositional matrix adjust. Identities = 469/475 (98%), Positives = 473/475 (99%) Query: 1 MSKNFDVVVIGGGPGGYIAAIRAAQLGFNTACIDEWKNEKGGPAPGGTCTNVGCIPSKAL 60 MSKNFDVVVIGGGPGGYIAAIRAAQLGFNTACIDEWKNEKGGPAPGGTCTNVGCIPSKAL Sbjct: 1 MSKNFDVVVIGGGPGGYIAAIRAAQLGFNTACIDEWKNEKGGPAPGGTCTNVGCIPSKAL 60 Query: 61 LQSSEHYEHASHGFAEHGIEVKGLGLNLEKMLGRKNTVVKQNNDGILYLFKKNKVAFFHG 120 LQSSEHYEHASHGFAEHGIEVKGLGLNLEKMLGRKNTVVKQNNDGILYLFKKNKV+FFHG Sbjct: 61 LQSSEHYEHASHGFAEHGIEVKGLGLNLEKMLGRKNTVVKQNNDGILYLFKKNKVSFFHG 120 Query: 121 RGSFVKGDANGYEIKVAGAAEETITAKHVIVATGSNARALPGAEFDEKLILSNTGALSIS 180 RGSFVKGDANGYEIKVAGAAEETITAKHVIVATGSNARALPGAEFDEKLILSNTGALSI+ Sbjct: 121 RGSFVKGDANGYEIKVAGAAEETITAKHVIVATGSNARALPGAEFDEKLILSNTGALSIT 180 Query: 181 EVPKKLGVIGAGVIGLEMGSVWRRLGAEVTVLEALPTFLGAVDEQIAKEAHKLLTKQGLA 240 EVPKKLGVIGAGVIGLEMGSVWRRLGAEVTVLEALPTFLGAVDEQIAKEA KLLTKQGLA Sbjct: 181 EVPKKLGVIGAGVIGLEMGSVWRRLGAEVTVLEALPTFLGAVDEQIAKEAQKLLTKQGLA 240 Query: 241 VNLGVKIGAIKTGKNSVSVEYTDAKGEAHTGEFDKLIISIGRTPNTIGLNAEGVGLKLDE 300 VNLGVKIGAIK GKNSVSVEYTDAKG+AHTGEFDKLI+SIGRTPNTIGLNAEGVGLKLDE Sbjct: 241 VNLGVKIGAIKAGKNSVSVEYTDAKGDAHTGEFDKLIVSIGRTPNTIGLNAEGVGLKLDE 300 Query: 301 RGFIAVDGDCKTNLPNVWAVGDVVRGPMLAHKAEEEGVAVAERIAGQHGHVNFNTIPWVI 360 RGFIAVDGDCKTNLPNVWAVGDVVRGPMLAHKAEEEGVAVAERIAGQHGHVNFNTIPWVI Sbjct: 301 RGFIAVDGDCKTNLPNVWAVGDVVRGPMLAHKAEEEGVAVAERIAGQHGHVNFNTIPWVI 360 Query: 361 YTSPEIAWVGKTEQQLKAEGVQYKAGTFPFLANGRARALGDTSGMVKFLADAKTDEILGV 420 YTSPEIAWVGKTEQQLKAEGVQYKAGTFPFLANGRARALGDTSGMVKFLADAKTDEILGV Sbjct: 361 YTSPEIAWVGKTEQQLKAEGVQYKAGTFPFLANGRARALGDTSGMVKFLADAKTDEILGV 420 Query: 421 HIVGPMASELISEAVVAMEFRASSEDIARICHAHPSLSEATKEAALAVDKRALNF 475 HIVGPMASELISEAVVAMEFRASSEDIARICHAHPSLSEATKEAALAVDKRALNF Sbjct: 421 HIVGPMASELISEAVVAMEFRASSEDIARICHAHPSLSEATKEAALAVDKRALNF 475 >gi|300310787|ref|YP_003774879.1| 2-oxoglutarate dehydrogenase E3 component lipoamide dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 596 Score = 308 bits (790), Expect = 4e-85, Method: Compositional matrix adjust. Identities = 179/463 (38%), Positives = 267/463 (57%), Gaps = 13/463 (2%) Query: 6 DVVVIGGGPGGYIAAIRAAQLGFNTACIDEWKNEKGGPAPGGTCTNVGCIPSKALLQSSE 65 DV+V+GGGPGGY AA RAA LG NT ++ GG C NVGCIPSKALL + Sbjct: 128 DVMVLGGGPGGYSAAFRAADLGLNTVIVEREATL------GGVCLNVGCIPSKALLHVAA 181 Query: 66 HYEHASHGFAEHGIEVKGLGLNLEKMLGRKNTVVKQNNDGILYLFKKNKVAFFHGRGSFV 125 + + A HG+ ++++K+ K+ V+ G+ + K KV +G G F Sbjct: 182 VIDETA-AMASHGVTFGKPQIDIDKLRAYKDKVIGTMTGGLAGMAKARKVQVVNGDGQF- 239 Query: 126 KGDANGYEIKVAGAAEETITAKHVIVATGSNARALPGAEFDEKLILSNTGALSISEVPKK 185 N E+ A +++ + KH I+A GS+ LP D + I+ +TGAL + +VPK+ Sbjct: 240 -AGPNHIEVTAADGSKKVVQFKHAIIAAGSSVVNLPFVPQDPR-IVDSTGALELRQVPKR 297 Query: 186 LGVIGAGVIGLEMGSVWRRLGAEVTVLEALPTFLGAVDEQIAKEAHKLLTKQGLAVNLGV 245 + VIG G+IGLEM +V+ LGA + V+E + + D + K K K+ V + Sbjct: 298 MLVIGGGIIGLEMATVYSTLGARIDVVEMMDGLMQGADRDMVKVWQKFNEKRFDNVMVKT 357 Query: 246 KIGAIKTGKNSVSVEYTDAKGEAHTGE---FDKLIISIGRTPNTIGLNAEGVGLKLDERG 302 K A++ + V + A+ A E +D +++++GR+PN L+A+ G+ + +RG Sbjct: 358 KTVAVEALPEGIKVTFEAAEAGATAPEPQLYDLVLVAVGRSPNGKKLSADKAGVIVSDRG 417 Query: 303 FIAVDGDCKTNLPNVWAVGDVVRGPMLAHKAEEEGVAVAERIAGQHGHVNFNTIPWVIYT 362 FIAVD +TN+P+++A+GD+V PMLAHKA EG AE IAG+ + + IP V YT Sbjct: 418 FIAVDKQMRTNVPHIFAIGDLVGQPMLAHKAVHEGHVAAEAIAGEKSFFDASVIPSVAYT 477 Query: 363 SPEIAWVGKTEQQLKAEGVQYKAGTFPFLANGRARALGDTSGMVKFLADAKTDEILGVHI 422 PE+AWVG TE + KA+G++ + G FP+ A+GRA A G + G K L DA+T I+G I Sbjct: 478 DPEVAWVGVTEDEAKAKGIKIEKGHFPWAASGRAVANGRSEGFTKLLFDAETHRIIGGGI 537 Query: 423 VGPMASELISEAVVAMEFRASSEDIARICHAHPSLSEATKEAA 465 VG A ++I E +A+E A + DI + H HP+L E+ AA Sbjct: 538 VGTHAGDMIGEVALAIEMGADAVDIGKTIHPHPTLGESLGMAA 580 >gi|300312608|ref|YP_003776700.1| glutathione reductase [Herbaspirillum seropedicae SmR1] Length = 458 Score = 167 bits (423), Expect = 2e-42, Method: Compositional matrix adjust. Identities = 141/471 (29%), Positives = 233/471 (49%), Gaps = 35/471 (7%) Query: 1 MSK-NFDVVVIGGGPGGYIAAIRAAQLGFNTACIDEWKNEKGGPAPGGTCTNVGCIPSKA 59 MS+ ++D+ IGGG GG AA A+Q G EKG GGTC N+GCIP K Sbjct: 1 MSQYDYDLFTIGGGSGGVRAARFASQAGARVGLA-----EKGDL--GGTCVNLGCIPKK- 52 Query: 60 LLQSSEHYEHASHGFAEHGIEVKGL-GLNLEKMLGRKNTVVKQNNDGILYLFKKNKVAFF 118 L+ S HY A +G + G + ++ K+ + ND L L KV+ Sbjct: 53 LMSYSAHYHEEFADAAGYGWTLNGQPSFDWSALMANKDREIAHLNDIYLRLLDNAKVSLH 112 Query: 119 HGRGSFVKGDANGYEIKVAGAAEETITAKHVIVATG--SNARALPGAEFDEKLILSNTGA 176 G F K + + + + V G + TA+H++VATG + A+PGAE L +++ Sbjct: 113 RG---FAKVE-DAHTVNVDG---QRFTARHILVATGGRPDKPAIPGAE----LGITSDDF 161 Query: 177 LSISEVPKKLGVIGAGVIGLEMGSVWRRLGAEVTVLEALPTFLGAVDEQIAKEAHKLLTK 236 + +P++ V+G G I +E+ S+ LG EVT++ L +D + + K Sbjct: 162 FHLKALPQRAVVLGGGYIAVELASILNGLGCEVTLVYRRERLLRNMDADLGIHLADEMAK 221 Query: 237 QGLAVNLGVKIGAIKTGK----NSVSVEYTD-AKGEAHTGEFDKLIISIGRTPNTIGLNA 291 +G+ + I AI+ + + SV+ GEA + + ++ + GRT NT GL Sbjct: 222 KGIRIVFNASIEAIEAEQAGEASEASVKTVRLTNGEALSAQC--VLFATGRTANTAGLGL 279 Query: 292 EGVGLKLDERGFIAVDGDCKTNLPNVWAVGDVVRGPMLAHKAEEEGVAVAERIAGQHGH- 350 + G+KL G I V+ D ++++P++ AVGDV+ L A E +AV R+ G+ Sbjct: 280 QEAGVKLKANGAIEVNQDFESSVPSILAVGDVIDRVALTPVALAEAMAVVSRLFGKGERG 339 Query: 351 VNFNTIPWVIYTSPEIAWVGKTEQQLKAEGVQYK--AGTFPFLANGRARALGDTSGMVKF 408 +++ IP +++ P + VG +E++ + + + + F L N +R T +K Sbjct: 340 MSYANIPTAVFSHPNVGTVGLSEEEARQQFGELRIFKTDFKPLKNTLSRNTERT--FMKL 397 Query: 409 LADAKTDEILGVHIVGPMASELISEAVVAMEFRASSEDIARICHAHPSLSE 459 + DA+TD +LGVH+VG A E+I VA++ A+ HP+ +E Sbjct: 398 VVDARTDRVLGVHMVGGDAGEVIQGFAVALQCGATKAQFDTTIGIHPTSAE 448 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.134 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,473,247 Number of extensions: 64236 Number of successful extensions: 255 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 248 Number of HSP's successfully gapped: 4 Length of query: 475 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 386 Effective length of database: 1,198,455 Effective search space: 462603630 Effective search space used: 462603630 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 132 (55.5 bits)