BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2880.p (347 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312410|ref|YP_003776502.1| dienelactone hydrolase [Herbasp... 72 8e-14 >gi|300312410|ref|YP_003776502.1| dienelactone hydrolase [Herbaspirillum seropedicae SmR1] Length = 345 Score = 71.6 bits (174), Expect = 8e-14, Method: Compositional matrix adjust. Identities = 62/219 (28%), Positives = 95/219 (43%), Gaps = 47/219 (21%) Query: 91 GRLPAVVIMHSSAG-------------------------VSAAEEDWSNFFNEMGLATLV 125 G PAVV++H AG +S + W+ ++ E G L+ Sbjct: 95 GPHPAVVMLHGRAGPYSSRINGDCTRIGPALPSPCDASTLSQRHQAWAAYWIERGYLALL 154 Query: 126 VDSFTPR--------HIQRSVEDQNVLNVAASAMDALKALELLATHPAIDPARIAVIGFS 177 VDS+ PR H + E V + +DA AL LA ++PARI + G+S Sbjct: 155 VDSYGPRGVAHGFGRHTHGAPERAEVNELTVRPLDAEGALAWLAPRSDVNPARILLQGWS 214 Query: 178 RGGGAGLHASFERVRQAAIADDT--RFAIHLLFYPSCSEFARTTGA-----PLHLFMGSE 230 G L+ RQA+ T FA ++FYP C A + + P+ + +G+ Sbjct: 215 NGASTALNV---MQRQASRLGGTAPSFAGAMVFYPGCGPQALLSSSPELDRPVQVLLGAA 271 Query: 231 DAYNSVEKCRFNVEQ---LRRVGAKVELTVYDGAKHGFD 266 D S ++C + Q +RR + V +T+Y GA H FD Sbjct: 272 DQEVSPQRCLRVLRQAIPMRRNSSPV-VTLYPGATHDFD 309 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.135 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,078,359 Number of extensions: 44478 Number of successful extensions: 133 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 130 Number of HSP's successfully gapped: 1 Length of query: 347 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 261 Effective length of database: 1,212,660 Effective search space: 316504260 Effective search space used: 316504260 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 131 (55.1 bits)