BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2864.p (479 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312825|ref|YP_003776917.1| NAD-dependent aldehyde dehydrog... 851 0.0 gi|300310355|ref|YP_003774447.1| NAD-dependent aldehyde dehydrog... 246 3e-66 gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrog... 245 5e-66 gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semial... 243 3e-65 gi|300311221|ref|YP_003775313.1| NAD+ dependent acetaldehyde deh... 241 8e-65 >gi|300312825|ref|YP_003776917.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 476 Score = 851 bits (2198), Expect = 0.0, Method: Compositional matrix adjust. Identities = 437/475 (92%), Positives = 452/475 (95%) Query: 5 HIASNIIGGQLHPGSRLLDSHNPANGQVMGRFPQSDASEVAAAVAAARRAQAAWAAQSLQ 64 HIASNIIGGQL GSR DSHNPA G VMGRFPQSDA EVAAAVAAAR+AQ AWAA SL Sbjct: 2 HIASNIIGGQLRAGSRQRDSHNPATGAVMGRFPQSDAIEVAAAVAAARQAQPAWAALSLV 61 Query: 65 ERREHLHRVADAIAANAERLATLVTLEQGRPLGGVGPDQVPGSRFELWGCEAWTRATASL 124 +RRE+L+RVADAIAANAERLATLVT EQGRPLGGVGPDQVPGSRFELWGCEAWTRATASL Sbjct: 62 QRREYLNRVADAIAANAERLATLVTQEQGRPLGGVGPDQVPGSRFELWGCEAWTRATASL 121 Query: 125 ELASEVVFEDETRRDELHRKPYGVIAAIAPWNWPLMISIWQVIPAIYAGNTVVLKPSEYT 184 ELA EVVFEDETRRDELHRKPYGVIAAIAPWNWPLMISIWQVIPA+YAGNTVVLKPSEYT Sbjct: 122 ELAPEVVFEDETRRDELHRKPYGVIAAIAPWNWPLMISIWQVIPALYAGNTVVLKPSEYT 181 Query: 185 SVCGLELARVIAQALPPGVLNTVSGDGSVGAMLVEHSDVNKIMFTGSGATGARIVAAAAR 244 SVCGLELARV+A ALPPGVLNTVSGDGSVGAMLVE DVNKIMFTGSGATGARIVAAAAR Sbjct: 182 SVCGLELARVVADALPPGVLNTVSGDGSVGAMLVEDGDVNKIMFTGSGATGARIVAAAAR 241 Query: 245 NLTPTTMELGGNDAAIVLDDADPKAIAPGLFWGAFLNMGQTCACAKRLYVPDALHDQIVA 304 NLTPTTMELGGNDAAIVLDDADPKAIAPGLFWGAFLNMGQTCACAKRLYVPDALHDQIV Sbjct: 242 NLTPTTMELGGNDAAIVLDDADPKAIAPGLFWGAFLNMGQTCACAKRLYVPDALHDQIVD 301 Query: 305 ELKAIIAATPMGDGLQPGMAMGPVQNPMQLKKVAALVDSARQEGATIVCGGVLPEGPGNF 364 ELKAIIAA PMGDGLQPG+AMGPVQNPMQLKKV ALV+SAR+EG TIVCGGVLP+GPGNF Sbjct: 302 ELKAIIAALPMGDGLQPGIAMGPVQNPMQLKKVVALVESAREEGGTIVCGGVLPDGPGNF 361 Query: 365 YPLTLVTDIRDGARLVDEEQFGPVLPIIRYSTLDEAIANANRLDVGLGASVWSRDAQRAA 424 YPLTLVT +RDGARLVDEEQFGPVLPIIRYS L++AIANANRLDVGLGASVWS D+QRAA Sbjct: 362 YPLTLVTGLRDGARLVDEEQFGPVLPIIRYSDLEQAIANANRLDVGLGASVWSSDSQRAA 421 Query: 425 QVATRLQAGTVWVNQHGMVHPMVPFGGNKRSGWGLEFGNEGLKGVTQPQVISLKK 479 QVATRLQAGTVWVNQHGMVHPMVPFGGNKRSGWG+EFG EGLKGVTQPQVISLKK Sbjct: 422 QVATRLQAGTVWVNQHGMVHPMVPFGGNKRSGWGVEFGQEGLKGVTQPQVISLKK 476 >gi|300310355|ref|YP_003774447.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 793 Score = 246 bits (628), Expect = 3e-66, Method: Compositional matrix adjust. Identities = 168/450 (37%), Positives = 231/450 (51%), Gaps = 20/450 (4%) Query: 9 NIIGGQLHPGSRLLDSHNPANGQVMGRFPQSDASEVAAAVAAARRAQAAWAAQSLQERRE 68 + I G+ LD +PA+G+++ Q A++V AAV AA+ A W A R Sbjct: 35 HFIAGRFTQPKADLDDIDPASGKLLAHLSQGSAADVDAAVQAAQAALPGWQALGGHGRAR 94 Query: 69 HLHRVADAIAANAERLATLVTLEQGRPLGGVGPDQVPGSRFELWGCEAWTRATASLELAS 128 HL+ +A + +A LA L TL+ G+PL VP + W + Sbjct: 95 HLYALARTVQRHARLLAVLETLDNGKPLRESRDVDVPLVARHFYHYAGWAQL-------Q 147 Query: 129 EVVFEDETRRDELHRKPYGVIAAIAPWNWPLMISIWQVIPAIYAGNTVVLKPSEYTSVCG 188 E F D P GV+ I PWN+PL++ W++ PA+ GNTVVLKP+E TS+ Sbjct: 148 ESEFPDHV--------PVGVVGQIVPWNFPLLMLAWKIAPALALGNTVVLKPAENTSLTA 199 Query: 189 LELARVIAQA-LPPGVLNTVSGDGSVGAMLVEHSDVNKIMFTGSGATGARIVAAAARNLT 247 L A + QA LP GVLN V+GDG+ GA +V H + KI FTGS G I A + Sbjct: 200 LLFAELAQQAGLPAGVLNIVTGDGATGAAVVAHPGIQKIAFTGSTEVGRLIREQTAGSGK 259 Query: 248 PTTMELGGNDAAIVLDDADPKAIAPGLFWGAFLNMGQTCACAKRLYVPDALHDQIVAELK 307 T+ELGG IV +DAD A G+ + N GQ C RL V + +HD +A LK Sbjct: 260 SLTLELGGKSPFIVFEDADIDAAIEGVVDAIWFNQGQVCCAGSRLLVQEGIHDLFIARLK 319 Query: 308 AIIAATPMGDGLQPGMAMGPVQNPMQLKKVAALVDSARQEGATIVCGGVLPEGP--GNFY 365 + +G L MG + +P+QL++V +LV+ +EGA C V+ + P G FY Sbjct: 320 TRMQKLKVGAPLDKCSDMGALISPVQLERVRSLVEQGVREGAQ--CHQVVLDTPPGGCFY 377 Query: 366 PLTLVTDIRDGARLVDEEQFGPVLPIIRYSTLDEAIANANRLDVGLGASVWSRDAQRAAQ 425 P TL+T + A + EE FGPVL + + T DEA+ AN GL AS+WS A Sbjct: 378 PPTLLTGVHPAATVASEEIFGPVLVAMSFRTPDEAVQLANNSRYGLAASIWSETIGLALG 437 Query: 426 VATRLQAGTVWVNQHGMVHPMVPFGGNKRS 455 VA +L+AG VW+N V FGG + S Sbjct: 438 VAPQLKAGVVWINSTNQFDAAVGFGGVRES 467 >gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 477 Score = 245 bits (626), Expect = 5e-66, Method: Compositional matrix adjust. Identities = 151/459 (32%), Positives = 236/459 (51%), Gaps = 7/459 (1%) Query: 20 RLLDSHNPANGQVMGRFPQSDASEVAAAVAAARRAQAAWAAQSLQERREHLHRVADAIAA 79 R +D NPA +V+G+ +D +++ A+ AA + W S ER + + R AD + Sbjct: 20 RTIDVINPATEEVIGKIAHADRADLDRALEAASKGFETWRNMSAFERSKIMRRAADLLRE 79 Query: 80 NAERLATLVTLEQGRPLGGVGPDQVPGSRFELWGCEAWTRATASLELASEVVFEDETRRD 139 AE +A L+T+EQG+PL + + + W E R L V Sbjct: 80 RAEEVARLMTMEQGKPLAEAKMETLGAADTIDWFAEEARRTYGRL-----VPARVPGAYQ 134 Query: 140 ELHRKPYGVIAAIAPWNWPLMISIWQVIPAIYAGNTVVLKPSEYTSVCGLELARVIAQA- 198 + ++P G +AA PWN+PL + ++ A+ AG ++++K E T EL R A A Sbjct: 135 MVIKEPVGPVAAFTPWNFPLNQVVRKLSAALAAGCSIIVKAPEETPASPAELIRAYADAG 194 Query: 199 LPPGVLNTVSG-DGSVGAMLVEHSDVNKIMFTGSGATGARIVAAAARNLTPTTMELGGND 257 +P GV+N V G + L+ H + KI FTGS G ++ A A +++ TMELGG+ Sbjct: 195 VPAGVINLVYGVPAEISEYLIPHPVIKKISFTGSTPVGKQLAALAGQHMKRATMELGGHA 254 Query: 258 AAIVLDDADPKAIAPGLFWGAFLNMGQTCACAKRLYVPDALHDQIVAELKAIIAATPMGD 317 A+V DDAD A L F N GQ C R V + + V + +G+ Sbjct: 255 PAMVFDDADVDVAAKMLATAKFRNAGQVCVSPTRFLVQKGVFNAFVDKFVTYAEQLKVGN 314 Query: 318 GLQPGMAMGPVQNPMQLKKVAALVDSARQEGATIVCGGVLPEGPGNFYPLTLVTDIRDGA 377 GL+ G+ MGPV N ++ + AL+D A +GA + GG G F+ T++T++ A Sbjct: 315 GLEAGITMGPVANKRRIPALEALIDDAVSQGAQLRTGGKRIGNKGYFFQPTVLTNLPLQA 374 Query: 378 RLVDEEQFGPVLPIIRYSTLDEAIANANRLDVGLGASVWSRDAQRAAQVATRLQAGTVWV 437 R ++EE FGP+ I + TLDE I ANRL GL A +++ A +A R+++G + + Sbjct: 375 RAMNEEPFGPLALINSFETLDEVITEANRLPYGLAAYAFAKSAHTKHALAARVESGMLTI 434 Query: 438 NQHGMVHPMVPFGGNKRSGWGLEFGNEGLKGVTQPQVIS 476 N G+ P +PFGG K SG+G E G E ++ ++++ Sbjct: 435 NHLGLALPELPFGGIKDSGYGSEGGTEAIEAYINTKLVT 473 >gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 243 bits (619), Expect = 3e-65, Method: Compositional matrix adjust. Identities = 152/447 (34%), Positives = 234/447 (52%), Gaps = 7/447 (1%) Query: 22 LDSHNPANGQVMGRFPQSDASEVAAAVAAARRAQAAWAAQSLQERREHLHRVADAIAANA 81 L HNPANG+++G P A+E A+ AA A AW ++ +ER L R + + AN Sbjct: 29 LAVHNPANGELLGHVPLMGAAETRRAIEAANAAWPAWKKKTAKERSAILRRWYELMLANT 88 Query: 82 ERLATLVTLEQGRPLGGVGPDQVPGSRFELWGCEAWTRATASLELASEVVFEDETRRDEL 141 + LA ++T EQG+PL + + F W E R + + R + Sbjct: 89 DDLALIMTAEQGKPLAEARGEIGYAASFIEWFAEEGKRT-----YGDTIPSPSPSNRIVV 143 Query: 142 HRKPYGVIAAIAPWNWPLMISIWQVIPAIYAGNTVVLKPSEYTSVCGLELARVIAQA-LP 200 ++ GV AAI PWN+P + + PA+ AG +VLKP+E T L LA + +A +P Sbjct: 144 IKEAIGVCAAITPWNFPAAMITRKAGPALAAGCPMVLKPAEATPFSALALAVLAERAGIP 203 Query: 201 PGVLNTVSGD-GSVGAMLVEHSDVNKIMFTGSGATGARIVAAAARNLTPTTMELGGNDAA 259 GV + V+G +G + + V KI FTGS G ++ +A ++ ++ELGGN Sbjct: 204 AGVFSVVTGTPKGIGGEMTSNPIVRKISFTGSTGVGKLLMQQSASSIKKLSLELGGNAPF 263 Query: 260 IVLDDADPKAIAPGLFWGAFLNMGQTCACAKRLYVPDALHDQIVAELKAIIAATPMGDGL 319 IV DDAD A G + N GQTC CA R+YV D ++D A+L + +G G Sbjct: 264 IVFDDADLDAAVEGAIASKYRNAGQTCVCANRIYVQDGVYDAFAAKLVEAVKKFKVGQGT 323 Query: 320 QPGMAMGPVQNPMQLKKVAALVDSARQEGATIVCGGVLPEGPGNFYPLTLVTDIRDGARL 379 + G+ GP+ N ++KV V A +GA ++ GG +F+ T++ D+ ++ Sbjct: 324 EEGVTQGPLINEQAVQKVEQHVADAVAKGARVLLGGKRHALGHSFFEPTVLADVTPAMQV 383 Query: 380 VDEEQFGPVLPIIRYSTLDEAIANANRLDVGLGASVWSRDAQRAAQVATRLQAGTVWVNQ 439 EE FGP+ P+ R+ T +E +A AN + GL + +SRD R +VA L++G V +N Sbjct: 384 AREETFGPMAPLFRFKTDEEVLALANDTEFGLASYFYSRDIGRIWRVAEGLESGMVGINT 443 Query: 440 HGMVHPMVPFGGNKRSGWGLEFGNEGL 466 + + + PFGG K+SG G E + G+ Sbjct: 444 GLISNEVAPFGGVKQSGLGREGSHYGI 470 >gi|300311221|ref|YP_003775313.1| NAD+ dependent acetaldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 506 Score = 241 bits (615), Expect = 8e-65, Method: Compositional matrix adjust. Identities = 154/466 (33%), Positives = 237/466 (50%), Gaps = 21/466 (4%) Query: 9 NIIGGQLHPGSR--LLDSHNPANGQVMGRFPQSDASEVAAAVAAARRAQAAWAAQSLQER 66 N IGG+ P + ++ +P G+ +S A +V A+ AA A+ +W S ER Sbjct: 21 NFIGGKFVPPVKGEYFENISPVIGRAFCEVARSSAEDVELALDAAHAAKKSWGKTSPTER 80 Query: 67 REHLHRVADAIAANAERLATLVTLEQGRPLGGVGPDQVPGS--RFELWGCEAWTRATASL 124 L ++AD + AN E LAT TL+ G+P+ +P + F + T+ Sbjct: 81 ANMLLKIADRMEANLELLATAETLDNGKPIRETMAADIPLAIDHFRYFAAAVRTQE---- 136 Query: 125 ELASEVVFEDETRRDELHRKPYGVIAAIAPWNWPLMISIWQVIPAIYAGNTVVLKPSEYT 184 S +++T H +P GV+ I PWN+P+++++W++ PA+ AGN VVLKP+E T Sbjct: 137 --GSICPIDNDTYAYHFH-EPLGVVGQIIPWNFPILMAVWKLAPALAAGNCVVLKPAEQT 193 Query: 185 SVCGLELARVIAQALPPGVLNTVSGDG-SVGAMLVEHSDVNKIMFTGSGATGARIVAAAA 243 + L +IA +PPGV+N V G G G L + + KI FTG TG I+ A+ Sbjct: 194 PASIMVLIELIADLIPPGVVNIVQGFGVEAGKPLASNKRIAKIAFTGETTTGRLIMQYAS 253 Query: 244 RNLTPTTMELGGNDAAIVLDDA-----DPKAIAPGLFWGAFLNMGQTCACAKRLYVPDAL 298 +NL P T+ELGG I D D A F LN G+ C C R+ V +++ Sbjct: 254 QNLIPVTLELGGKSPNIFFADVLDKDDDFFDKALEGFAMFALNQGEVCTCPSRVLVQESI 313 Query: 299 HDQIVAELKAIIAATPMGDGLQPGMAMGPVQNPMQLKKVAALVDSARQEGATIVCGGVLP 358 +++ + +AA G+ L +G + QL+K+ + +D +QEGA ++ GG Sbjct: 314 YERFIERALKRVAAIKQGNPLDKSTMIGAQASQEQLEKILSYIDIGKQEGAKVLAGGGRE 373 Query: 359 EGPGN----FYPLTLVTDIRDGARLVDEEQFGPVLPIIRYSTLDEAIANANRLDVGLGAS 414 E G+ +Y V + R+ EE FGPV+ + + +EA+A AN GLGA Sbjct: 374 ELGGDLASGYYVKPTVFQGNNKMRIFQEEIFGPVVSVTTFKDEEEALAIANDTLYGLGAG 433 Query: 415 VWSRDAQRAAQVATRLQAGTVWVNQHGMVHPMVPFGGNKRSGWGLE 460 +W+RD RA ++ +QAG VW N + + FGG K+SG G E Sbjct: 434 LWTRDGTRAFRMGREIQAGRVWTNCYHLYPAHAAFGGYKQSGIGRE 479 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.134 0.405 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,568,183 Number of extensions: 64456 Number of successful extensions: 300 Number of sequences better than 1.0e-08: 19 Number of HSP's gapped: 241 Number of HSP's successfully gapped: 20 Length of query: 479 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 390 Effective length of database: 1,198,455 Effective search space: 467397450 Effective search space used: 467397450 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)